Rh5DG049200

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002149
Physical Location & Seq
Reverse (-)
4092079 .. 4095651
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG049200.1

Sequence Viewer

Length: 1425 bp
ATGAACCAAAAGGCTAACGTTTCTAAAGAGCTTAATGCCAAGCACAGAAAGATATTGGAAGGTCTTCTTAAACTACCAGAGAACAGAGAATGTGCAGACTGCAAAGCCATAGCTCCACGATGGGCCAGTGTCAATCTTGGGATCTTTATATGCATGCAGTGTTCTGGGGCCCATAGAAGTCTTGGGGTACACATATCAAAGGTGAGATCTGCCACTTTGGACACGTGGCTTCCAGAGCAGATTGCATTTATCCAATCTATGGGAAATGAGAAATCGAACAGTTACTGGGAAGCAGAGTTGCCTCCTAATTATGACAGAGTTGGAATCGAGAATTTCATCCGTGCAAAGTATGAATTGAAAAAATGGGTCCCCAGGGATGGCAAACTAAAATCACCTCCCAGGGTGACTGCGCAGAAAACTTCTGTTTATGGAGCAAGGCCTGAAATAAGTAGTAAGCATGGCGATGCCAGAAATATTGAACAGTCTTATGAGCAGAAAGTTGCTCATCCACCTGTTAGAATAAACAGGATACCACAGCAGGTTAGTGATGATTTGAAACCGAAAAACATTGAAAAGAAATCACCAGAAGCTAGTCAAAAACCACGAGAACTTGAGCAGAAAACAGAACCATCAGCTCCAAAACCTGAACCAGCAAGTCATAGAGCTAGTGCTACTCCAATTTCCGCACCAGTTAAAGTGGACTATGCTACTGATTTGTTCAATATGCTCTCCATGGAGGAATCAAGAGGAAGTGACTCCGTGACATTTGCCAATAACATTACATTGGCTACTATGCATTCTGCTGAAACAAAACTGGCAGAAGAGAAAAACAATGCACCAAAATCATCTGAAAGCAAGGTGCAATCAAAATATGGTATTGAGGATTTGTTTATAGATTCTCCATCAATCACTCAAACCTTTTCAGTGAAACCTCAGACTGATGTCAAGAGTGACATTATAAACTTATTTGAGAAGTCAAGTATAGTTTCTCCATTCTCGATGCATCAACAACAACAACAAGCAATGCTCTCCCAACAACATTCATTACTAATGGCTGCTGCGCCAAATTCTAGCGGATCCCATTCCTTTTCTTCAAGTTTACAACCTGCCTCCCAAGCTATTCACTTGCCCACACAAAATGGGCAAAGCGTTGGCCAACAAGTTCCTGGCAACCCAACGCCGCCTAATCTACAATATTATAGTCAGATTGGGAATGGTTATCATGCACGTACAATAGGCAATGCAATTTCTTACTCGACATCGAGCATGTATACGATGAGGCCGGTAGCTCCAATCAACGGTGTCAGAAGCATCGGAGGAAGCAGAGTTTCAACAGCACCGCCAAAACCTTCTGTCACTCCAACTCCTTCGGGAAGGGACTTTGATTTTTCATCGTTAACTCAAGGATTGTTTACTAAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

474

Amino Acids

52.14

Weight (kDa)

9.39

Isoelectric Point (pI)

56.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 123 1.2e-39 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 411
Acc36I ACCTGC 2 cut(s) 529, 1114
AccB7I CCANNNNNTGG 1 cut(s) 259
AccI GTMKAC 1 cut(s) 1271
AciI CCGC 4 cut(s) 684, 1074, 1181, 1340
AclI AACGTT 1 cut(s) 18
AclWI GGATC 3 cut(s) 149, 1071, 1084
AcoI YGGCCR 1 cut(s) 1153
AcsI RAATTY 2 cut(s) 331, 1066
AcvI CACGTG 1 cut(s) 225
AfaI GTAC 2 cut(s) 189, 1231
AfiI CCNNNNNNNGG 3 cut(s) 259, 377, 1298
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 7 cut(s) 358, 479, 556, 572, 721, 1095, 1332
AjnI CCWGG 3 cut(s) 371, 398, 1165
AluBI AGCT 7 cut(s) 31, 113, 590, 635, 665, 1118, 1289
AluI AGCT 7 cut(s) 31, 113, 590, 635, 665, 1118, 1289
AlwI GGATC 3 cut(s) 149, 1071, 1084
AlwNI CAGNNNCTG 1 cut(s) 285
AoxI GGCC 5 cut(s) 123, 168, 437, 1153, 1280
ApaI GGGCCC 1 cut(s) 172
ApeKI GCWGC 2 cut(s) 1055, 1058
ApoI RAATTY 2 cut(s) 331, 1066
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 2 cut(s) 412, 1063
AspS9I GGNCC 4 cut(s) 123, 168, 169, 367
AsuHPI GGTGA 4 cut(s) 214, 384, 415, 573
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 1155
BamHI GGATCC 1 cut(s) 1076
BanII GRGCYC 1 cut(s) 172
BauI CACGAG 1 cut(s) 603
BbrPI CACGTG 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 1042, 1045
BccI CCATC 4 cut(s) 114, 371, 637, 910
BciT130I CCWGG 3 cut(s) 373, 400, 1167
BciVI GTATCC 1 cut(s) 522
BfaI CTAG 3 cut(s) 591, 666, 1071
BfuAI ACCTGC 2 cut(s) 529, 1114
BfuI GTATCC 1 cut(s) 522
BglII AGATCT 1 cut(s) 206
BisI GCNGC 3 cut(s) 1056, 1059, 1181
BlsI GCNGC 3 cut(s) 1057, 1060, 1182
Bme1390I CCNGG 3 cut(s) 373, 400, 1167
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 4 cut(s) 123, 168, 169, 367
BmiI GGNNCC 5 cut(s) 169, 170, 368, 369, 1078
BmrFI CCNGG 3 cut(s) 373, 400, 1167
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 4 cut(s) 454, 990, 1012, 1320
BmuI ACTGGG 1 cut(s) 295
BoxI GACNNNNGTC 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 56
BpuEI CTTGAG 2 cut(s) 632, 1386
BsaAI YACGTR 2 cut(s) 225, 1229
BsaJI CCNNGG 5 cut(s) 371, 372, 398, 399, 732
BsaXI ACNNNNNCTCC 2 cut(s) 1348, 1378
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 377, 1298
Bse118I RCCGGY 1 cut(s) 1282
Bse1I ACTGG 4 cut(s) 126, 290, 689, 819
Bse3DI GCAATG 2 cut(s) 1029, 1246
BseBI CCWGG 3 cut(s) 373, 400, 1167
BseDI CCNNGG 5 cut(s) 371, 372, 398, 399, 732
BseGI GGATG 3 cut(s) 336, 382, 505
BseLI CCNNNNNNNGG 3 cut(s) 259, 377, 1298
BseMI GCAATG 2 cut(s) 1029, 1246
BseMII CTCAG 1 cut(s) 947
BseNI ACTGG 4 cut(s) 126, 290, 689, 819
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 2 cut(s) 1042, 1045
BsgI GTGCAG 1 cut(s) 114
BshFI GGCC 5 cut(s) 125, 170, 439, 1155, 1282
BsiSI CCGG 1 cut(s) 1283
BslFI GGGAC 2 cut(s) 353, 1391
BslI CCNNNNNNNGG 3 cut(s) 259, 377, 1298
BsmFI GGGAC 2 cut(s) 353, 1391
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 5 cut(s) 125, 170, 439, 1155, 1282
Bsp120I GGGCCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 141, 206, 1076
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 4 cut(s) 684, 1074, 1181, 1340
BspANI GGCC 5 cut(s) 125, 170, 439, 1155, 1282
BspCNI CTCAG 1 cut(s) 946
BspLI GGNNCC 5 cut(s) 169, 170, 368, 369, 1078
BspMI ACCTGC 2 cut(s) 529, 1114
BspPI GGATC 3 cut(s) 149, 1071, 1084
BsrDI GCAATG 2 cut(s) 1029, 1246
BsrFI RCCGGY 1 cut(s) 1282
BsrI ACTGG 4 cut(s) 126, 290, 689, 819
BssAI RCCGGY 1 cut(s) 1282
BssECI CCNNGG 5 cut(s) 371, 372, 398, 399, 732
BssMI GATC 3 cut(s) 141, 206, 1076
BssNAI GTATAC 1 cut(s) 1272
BssSI CACGAG 1 cut(s) 603
BssT1I CCWWGG 1 cut(s) 732
Bst1107I GTATAC 1 cut(s) 1272
Bst2BI CACGAG 1 cut(s) 603
Bst2UI CCWGG 3 cut(s) 373, 400, 1167
Bst4CI ACNGT 3 cut(s) 281, 483, 1301
Bst6I CTCTTC 1 cut(s) 816
BstBAI YACGTR 2 cut(s) 225, 1229
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 933
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 3 cut(s) 336, 382, 505
BstHHI GCGC 2 cut(s) 412, 1063
BstKTI GATC 3 cut(s) 144, 209, 1079
BstMBI GATC 3 cut(s) 141, 206, 1076
BstMWI GCNNNNNNNGC 2 cut(s) 235, 1115
BstNI CCWGG 3 cut(s) 373, 400, 1167
BstNSI RCATGY 2 cut(s) 157, 1270
BstPAI GACNNNNGTC 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 371, 398, 1165
BstSLI GKGCMC 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 1042, 1045
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 141, 206, 1076
BstYI RGATCY 3 cut(s) 141, 206, 1076
BstZ17I GTATAC 1 cut(s) 1272
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 5 cut(s) 125, 170, 439, 1155, 1282
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 3 cut(s) 336, 382, 505
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 3 cut(s) 133, 164, 930
BveI ACCTGC 2 cut(s) 529, 1114
Cac8I GCNNGC 1 cut(s) 155
CaiI CAGNNNCTG 1 cut(s) 285
CfoI GCGC 2 cut(s) 412, 1063
Cfr10I RCCGGY 1 cut(s) 1282
Cfr13I GGNCC 4 cut(s) 123, 168, 169, 367
Csp6I GTAC 2 cut(s) 188, 1230
CviAII CATG 5 cut(s) 154, 458, 733, 1223, 1267
CviQI GTAC 2 cut(s) 188, 1230
DdeI CTNAG 1 cut(s) 933
DpnI GATC 3 cut(s) 143, 208, 1078
DpnII GATC 3 cut(s) 141, 206, 1076
EaeI YGGCCR 1 cut(s) 1153
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
Eco130I CCWWGG 1 cut(s) 732
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 367
Eco72I CACGTG 1 cut(s) 225
EcoO109I RGGNCCY 2 cut(s) 168, 367
EcoRII CCWGG 3 cut(s) 371, 398, 1165
EcoT14I CCWWGG 1 cut(s) 732
EcoT22I ATGCAT 3 cut(s) 155, 798, 1005
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 732
FaeI CATG 5 cut(s) 157, 461, 736, 1226, 1270
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 2 cut(s) 353, 1391
FatI CATG 5 cut(s) 153, 457, 732, 1222, 1266
FblI GTMKAC 1 cut(s) 1271
Fnu4HI GCNGC 3 cut(s) 1056, 1059, 1181
FokI GGATG 3 cut(s) 323, 389, 492
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 3 cut(s) 1056, 1059, 1181
FspBI CTAG 3 cut(s) 591, 666, 1071
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 2 cut(s) 411, 1062
GluI GCNGC 3 cut(s) 1056, 1059, 1181
HaeIII GGCC 5 cut(s) 125, 170, 439, 1155, 1282
HapII CCGG 1 cut(s) 1283
HhaI GCGC 2 cut(s) 412, 1063
Hin1II CATG 5 cut(s) 157, 461, 736, 1226, 1270
Hin6I GCGC 2 cut(s) 410, 1061
HinP1I GCGC 2 cut(s) 410, 1061
HincII GTYRAC 1 cut(s) 1398
HindII GTYRAC 1 cut(s) 1398
HinfI GANTC 4 cut(s) 324, 740, 755, 896
HpaI GTTAAC 1 cut(s) 1398
HpaII CCGG 1 cut(s) 1283
HphI GGTGA 4 cut(s) 214, 384, 415, 573
Hpy166II GTNNAC 6 cut(s) 190, 700, 1100, 1272, 1398, 1413
Hpy188I TCNGA 5 cut(s) 850, 936, 1206, 1307, 1316
Hpy188III TCNNGA 6 cut(s) 233, 328, 744, 946, 997, 1371
Hpy8I GTNNAC 6 cut(s) 190, 700, 1100, 1272, 1398, 1413
HpyAV CCTTC 4 cut(s) 53, 1359, 1368, 1377
HpyCH4III ACNGT 3 cut(s) 281, 483, 1301
HpyCH4IV ACGT 3 cut(s) 18, 224, 1228
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 1115
HpyF3I CTNAG 1 cut(s) 933
HpySE526I ACGT 3 cut(s) 18, 224, 1228
Hsp92II CATG 5 cut(s) 157, 461, 736, 1226, 1270
HspAI GCGC 2 cut(s) 410, 1061
KflI GGGWCCC 1 cut(s) 367
KspAI GTTAAC 1 cut(s) 1398
Kzo9I GATC 3 cut(s) 141, 206, 1076
LmnI GCTCC 4 cut(s) 118, 431, 640, 1294
Lsp1109I GCAGC 2 cut(s) 1042, 1045
LweI GCATC 4 cut(s) 454, 990, 1012, 1320
MaeI CTAG 3 cut(s) 591, 666, 1071
MaeII ACGT 3 cut(s) 18, 224, 1228
MaeIII GTNAC 6 cut(s) 281, 403, 752, 760, 950, 1354
MalI GATC 3 cut(s) 143, 208, 1078
MboI GATC 3 cut(s) 141, 206, 1076
MboII GAAGA 3 cut(s) 56, 833, 1083
MflI RGATCY 3 cut(s) 141, 206, 1076
MhlI GDGCHC 1 cut(s) 172
MlsI TGGCCA 1 cut(s) 1155
MluCI AATT 6 cut(s) 307, 331, 353, 678, 1066, 1245
MluNI TGGCCA 1 cut(s) 1155
MlyI GAGTC 1 cut(s) 749
MmeI TCCRAC 2 cut(s) 301, 1385
MnlI CCTC 9 cut(s) 312, 405, 730, 740, 874, 942, 1120, 1272, 1310
Mox20I TGGCCA 1 cut(s) 1155
Mph1103I ATGCAT 3 cut(s) 155, 798, 1005
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 1155
MseI TTAA 4 cut(s) 33, 69, 693, 1397
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 1155
MspI CCGG 1 cut(s) 1283
MspR9I CCNGG 3 cut(s) 373, 400, 1167
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 3 cut(s) 373, 400, 1167
MwoI GCNNNNNNNGC 2 cut(s) 235, 1115
NcoI CCATGG 1 cut(s) 732
NdeII GATC 3 cut(s) 141, 206, 1076
NlaIII CATG 5 cut(s) 157, 461, 736, 1226, 1270
NlaIV GGNNCC 5 cut(s) 169, 170, 368, 369, 1078
NmuCI GTSAC 5 cut(s) 403, 752, 760, 950, 1354
NsbI TGCGCA 1 cut(s) 411
NsiI ATGCAT 3 cut(s) 155, 798, 1005
NspI RCATGY 2 cut(s) 157, 1270
PaeI GCATGC 1 cut(s) 157
PasI CCCWGGG 2 cut(s) 372, 399
PceI AGGCCT 1 cut(s) 439
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 324, 740, 896
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 3 cut(s) 1057, 1060, 1182
PleI GAGTC 1 cut(s) 749
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
PpsI GAGTC 1 cut(s) 749
Ppu21I YACGTR 2 cut(s) 225, 1229
PpuMI RGGWCCY 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 941
PsiI TTATAA 1 cut(s) 959
Psp1406I AACGTT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 367
Psp6I CCWGG 3 cut(s) 371, 398, 1165
PspCI CACGTG 1 cut(s) 225
PspGI CCWGG 3 cut(s) 371, 398, 1165
PspN4I GGNNCC 5 cut(s) 169, 170, 368, 369, 1078
PspOMI GGGCCC 1 cut(s) 168
PspPI GGNCC 4 cut(s) 123, 168, 169, 367
PspPPI RGGWCCY 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 285
PsuI RGATCY 3 cut(s) 141, 206, 1076
RsaI GTAC 2 cut(s) 189, 1231
RsaNI GTAC 2 cut(s) 188, 1230
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 4 cut(s) 33, 69, 693, 1397
SatI GCNGC 3 cut(s) 1056, 1059, 1181
Sau3AI GATC 3 cut(s) 141, 206, 1076
Sau96I GGNCC 4 cut(s) 123, 168, 169, 367
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 3 cut(s) 373, 400, 1167
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 4 cut(s) 454, 990, 1012, 1320
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 611, 1401
SmoI CTYRAG 2 cut(s) 611, 1401
SphI GCATGC 1 cut(s) 157
Sse9I AATT 6 cut(s) 307, 331, 353, 678, 1066, 1245
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 4 cut(s) 684, 1074, 1181, 1340
SspI AATATT 2 cut(s) 475, 1196
SspMI CTAG 3 cut(s) 591, 666, 1071
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 3 cut(s) 371, 398, 1165
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 3 cut(s) 281, 483, 1301
TaiI ACGT 3 cut(s) 21, 227, 1231
TaqI TCGA 5 cut(s) 275, 327, 998, 1256, 1262
TasI AATT 6 cut(s) 307, 331, 353, 678, 1066, 1245
TauI GCSGC 1 cut(s) 1183
TfiI GAWTC 3 cut(s) 324, 740, 896
Tru1I TTAA 4 cut(s) 33, 69, 693, 1397
Tru9I TTAA 4 cut(s) 33, 69, 693, 1397
TscAI CASTG 3 cut(s) 133, 164, 930
TseFI GTSAC 5 cut(s) 403, 752, 760, 950, 1354
TseI GCWGC 2 cut(s) 1055, 1058
Tsp45I GTSAC 5 cut(s) 403, 752, 760, 950, 1354
TspDTI ATGAA 5 cut(s) 17, 325, 366, 1032, 1380
TspGWI ACGGA 2 cut(s) 329, 748
TspRI CASTG 3 cut(s) 133, 164, 930
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 331, 1066
XceI RCATGY 2 cut(s) 157, 1270
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 1163
XmiI GTMKAC 1 cut(s) 1271
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 3 cut(s) 591, 666, 1071
Zsp2I ATGCAT 3 cut(s) 155, 798, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.