MD10G1301800.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002149
Physical Location & Seq
Forward (+)
38877178 .. 38880245
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1301800.v1.1.491

Sequence Viewer

Length: 1470 bp
ATGAACCAGAAGGCGAGCGTTTCTAAAGAGCTTAATTCCAAACACAGAAAGATATTGGAGGGTCTTCTTAAACTACAAGAGAACAGAGAATGCGCAGACTGCAAAACCAAAGCTCCGCGATGGGCTAGCGTGAACCTTGGTATCTTCATATGCATGCAATGTTCAGGGATCCACAGGAGTCTTGGGGTACATATATCAAAGGTGAGATCTGCCAATTTGGATACATGGCTTCCGGAGCAGGTTGCATTTATCCAATCAATGGGAAATGATAAGGCGAATAGGTATTGGGAAGCAGAGTTGCCTCCTAAGTATGACAGAGTTGGAATTGAGAATTTCATTCGAGTAAAGTATGAACACAAAAAATGGGTTCCTAGAGATGGGAAAGTAAAATCACCTCCGAGGGTGAGTAAGGAGAAGCCTTCCGTTTATAGAGCGAGAAATGCGACTAGTAGTCAGCATGTCCATGTGAAAAAGATGGAACAGTTTTCTGAGGAGCAGAAAGGTGCTTGTACACTCAGTACACGTAACAGGACTACGCAGCAGATCAGTCGTGATCCAAAGCCACAAGAACTTGCACAGAAACCAAAACCACAAGAACTTGAACAGAAACCAAAACCGCAAGAACTTGATCAGAAACCTAAATCACAAGAACCAGCACAGAAACCAAAACCAGTAGTCTTGAAAGTTGAACCGGTAAGTGAGGCGGGTAGTGTTAGGCCAGTTTCCATACCAGTTAAAGTGGATTATGCTACTTATCTGTTCAATTTGCTCTCCATGGACGAAGATATAGAAAATGACTCGGTGGTATCTGCCAGTAAGAATAAATTGGCTGGTAACCATGTTGAAACAAAATTGATAGAAGAGAAAAGTAATAGACCAGAATCGTCTGAAAGCAAGATTCAACCAAAATATGATCTTGAAGATTTTTTTATAGATCCAACCCCAACCCCAATCACTCAACCCATTTCAATGAAAACTCAGAATGACGTGCAGAATGACATTATGAACTTCTTCGAGAAGTCCAGTATGGCTTCTCCATTCTCTGTGCATCAACAGCAACTTGCAATGTCATCCCAACAACAGGCATTACTCGTGGCTTCTGCGGCAAAATCTAGTGGATCCCATACCATTCCTGCAAACGCACAACCTGGCTCCCATTCTATTCAGCACTTACCCGCCCGAAATGGGCAAAACATTGGTCGCCAAGTTCCTGGAAGCCTGGTACATGTTCATCCACAACATTATACTCAAATTGGAAACAGCTATTATACACTCCCGGTAGGGAATACAGTTCCTTTCCAAACATCAAGCATGTACAGGACGAGGCCAGTAACTCCAGTTAATGGTGTCACAAACATCAGAGCTAACAAATTTTCATCAACGTCACCAATATCTTCTATCTCTTCAACTAAGTCAGGAAGGGATTTTGATTTCTCATCGTTAACTCAAGGATTGTTTACCAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

490

Amino Acids

54.76

Weight (kDa)

9.55

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 123 6.5e-41 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 94
Acc36I ACCTGC 1 cut(s) 229
AccB7I CCANNNNNTGG 2 cut(s) 259, 1343
AccII CGCG 1 cut(s) 118
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 5 cut(s) 116, 617, 704, 1103, 1176
AclWI GGATC 6 cut(s) 163, 176, 548, 929, 1113, 1126
AcsI RAATTY 2 cut(s) 331, 1370
AfaI GTAC 5 cut(s) 189, 511, 520, 1224, 1316
AfiI CCNNNNNNNGG 5 cut(s) 259, 377, 1081, 1185, 1343
AflIII ACRYGT 2 cut(s) 521, 1225
AgeI ACCGGT 1 cut(s) 691
AgsI TTSAA 9 cut(s) 602, 682, 689, 763, 845, 902, 920, 969, 1407
AhdI GACNNNNNGTC 1 cut(s) 450
AhlI ACTAGT 1 cut(s) 446
AjiI CACGTC 1 cut(s) 988
AjnI CCWGG 3 cut(s) 1147, 1210, 1218
AluBI AGCT 4 cut(s) 31, 113, 1263, 1364
AluI AGCT 4 cut(s) 31, 113, 1263, 1364
AlwI GGATC 6 cut(s) 163, 176, 548, 929, 1113, 1126
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 2 cut(s) 716, 1325
ApeKI GCWGC 1 cut(s) 538
ApoI RAATTY 2 cut(s) 331, 1370
ArsI GACNNNNNNTTYG 2 cut(s) 1183, 1215
AsiGI ACCGGT 1 cut(s) 691
Asp700I GAANNNNTTC 1 cut(s) 1010
AspLEI GCGC 1 cut(s) 95
AsuC2I CCSGG 1 cut(s) 1277
AsuHPI GGTGA 4 cut(s) 214, 384, 415, 1377
AsuNHI GCTAGC 1 cut(s) 125
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BamHI GGATCC 2 cut(s) 168, 1118
BauI CACGAG 1 cut(s) 1091
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 550
BccI CCATC 3 cut(s) 114, 371, 469
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 3 cut(s) 1149, 1212, 1220
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 1277
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 126, 372, 447, 1113
BfuAI ACCTGC 1 cut(s) 229
BfuI GTATCC 1 cut(s) 214
BglII AGATCT 1 cut(s) 206
BisI GCNGC 2 cut(s) 539, 1104
BlsI GCNGC 2 cut(s) 540, 1105
Bme1390I CCNGG 4 cut(s) 1149, 1212, 1220, 1277
BmeRI GACNNNNNGTC 1 cut(s) 450
BmgBI CACGTC 1 cut(s) 988
BmiI GGNNCC 4 cut(s) 170, 369, 1120, 1153
BmrFI CCNGG 4 cut(s) 1149, 1212, 1220, 1277
BmsI GCATC 1 cut(s) 1057
BmtI GCTAGC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 1320
BpuEI CTTGAG 1 cut(s) 1431
BpuMI CCSGG 1 cut(s) 1277
BsaAI YACGTR 1 cut(s) 524
BsaJI CCNNGG 3 cut(s) 136, 398, 774
BsaWI WCCGGW 2 cut(s) 232, 691
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 377, 1081, 1185, 1343
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 7 cut(s) 671, 719, 731, 813, 1023, 1328, 1337
Bse3DI GCAATG 2 cut(s) 164, 1071
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 3 cut(s) 1149, 1212, 1220
BseDI CCNNGG 3 cut(s) 136, 398, 774
BseGI GGATG 2 cut(s) 1070, 1231
BseLI CCNNNNNNNGG 5 cut(s) 259, 377, 1081, 1185, 1343
BseMI GCAATG 2 cut(s) 164, 1071
BseMII CTCAG 3 cut(s) 480, 529, 992
BseNI ACTGG 7 cut(s) 671, 719, 731, 813, 1023, 1328, 1337
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 1 cut(s) 550
BsgI GTGCAG 1 cut(s) 1010
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 718, 1327
BshTI ACCGGT 1 cut(s) 691
BsiSI CCGG 3 cut(s) 233, 692, 1277
BslI CCNNNNNNNGG 5 cut(s) 259, 377, 1081, 1185, 1343
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 718, 1327
Bsp13I TCCGGA 1 cut(s) 232
Bsp1407I TGTACA 2 cut(s) 509, 1314
Bsp143I GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 5 cut(s) 116, 617, 704, 1103, 1176
BspANI GGCC 2 cut(s) 718, 1327
BspCNI CTCAG 3 cut(s) 481, 528, 991
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 4 cut(s) 170, 369, 1120, 1153
BspMI ACCTGC 1 cut(s) 229
BspOI GCTAGC 1 cut(s) 129
BspPI GGATC 6 cut(s) 163, 176, 548, 929, 1113, 1126
BsrDI GCAATG 2 cut(s) 164, 1071
BsrFI RCCGGY 1 cut(s) 691
BsrGI TGTACA 2 cut(s) 509, 1314
BsrI ACTGG 7 cut(s) 671, 719, 731, 813, 1023, 1328, 1337
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 3 cut(s) 136, 398, 774
BssMI GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
BssSI CACGAG 1 cut(s) 1091
BssT1I CCWWGG 2 cut(s) 136, 774
Bst2BI CACGAG 1 cut(s) 1091
Bst2UI CCWGG 3 cut(s) 1149, 1212, 1220
Bst4CI ACNGT 3 cut(s) 483, 1291, 1467
Bst6I CTCTTC 2 cut(s) 855, 1408
BstAUI TGTACA 2 cut(s) 509, 1314
BstBAI YACGTR 1 cut(s) 524
BstC8I GCNNGC 3 cut(s) 16, 127, 155
BstDEI CTNAG 5 cut(s) 306, 489, 515, 978, 1410
BstDSI CCRYGG 1 cut(s) 774
BstEII GGTNACC 1 cut(s) 833
BstF5I GGATG 2 cut(s) 1070, 1231
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 8 cut(s) 171, 209, 546, 556, 631, 916, 937, 1121
BstMBI GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
BstMWI GCNNNNNNNGC 5 cut(s) 99, 235, 440, 1054, 1103
BstNI CCWGG 3 cut(s) 1149, 1212, 1220
BstNSI RCATGY 4 cut(s) 157, 461, 1229, 1315
BstPI GGTNACC 1 cut(s) 833
BstSCI CCNGG 4 cut(s) 1147, 1210, 1218, 1275
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 550
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 4 cut(s) 168, 206, 934, 1118
BstXI CCANNNNNNTGG 1 cut(s) 1211
BstYI RGATCY 4 cut(s) 168, 206, 934, 1118
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 2 cut(s) 718, 1327
BtgI CCRYGG 1 cut(s) 774
BtgZI GCGATG 1 cut(s) 133
BtrI CACGTC 1 cut(s) 988
BtsCI GGATG 2 cut(s) 1070, 1231
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 3 cut(s) 16, 127, 155
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 691
Csp6I GTAC 5 cut(s) 188, 510, 519, 1223, 1315
CspAI ACCGGT 1 cut(s) 691
CviAII CATG 8 cut(s) 154, 225, 458, 464, 775, 839, 1226, 1312
CviQI GTAC 5 cut(s) 188, 510, 519, 1223, 1315
DdeI CTNAG 5 cut(s) 306, 489, 515, 978, 1410
DpnI GATC 8 cut(s) 170, 208, 545, 555, 630, 915, 936, 1120
DpnII GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
DriI GACNNNNNGTC 1 cut(s) 450
Eam1104I CTCTTC 2 cut(s) 855, 1408
Eam1105I GACNNNNNGTC 1 cut(s) 450
EarI CTCTTC 2 cut(s) 855, 1408
Eco130I CCWWGG 2 cut(s) 136, 774
Eco91I GGTNACC 1 cut(s) 833
EcoO65I GGTNACC 1 cut(s) 833
EcoRII CCWGG 3 cut(s) 1147, 1210, 1218
EcoT14I CCWWGG 2 cut(s) 136, 774
EcoT22I ATGCAT 1 cut(s) 155
ErhI CCWWGG 2 cut(s) 136, 774
FaeI CATG 8 cut(s) 157, 228, 461, 467, 778, 842, 1229, 1315
FatI CATG 8 cut(s) 153, 224, 457, 463, 774, 838, 1225, 1311
FauI CCCGC 2 cut(s) 697, 1183
FauNDI CATATG 1 cut(s) 149
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 539, 1104
FokI GGATG 2 cut(s) 1057, 1218
Fsp4HI GCNGC 2 cut(s) 539, 1104
FspBI CTAG 4 cut(s) 126, 372, 447, 1113
FspI TGCGCA 1 cut(s) 94
GlaI GCGC 1 cut(s) 94
GluI GCNGC 2 cut(s) 539, 1104
GsuI CTGGAG 1 cut(s) 1320
HaeIII GGCC 2 cut(s) 718, 1327
HapII CCGG 3 cut(s) 233, 692, 1277
HhaI GCGC 1 cut(s) 95
Hin1II CATG 8 cut(s) 157, 228, 461, 467, 778, 842, 1229, 1315
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 1 cut(s) 1443
HindII GTYRAC 1 cut(s) 1443
HinfI GANTC 4 cut(s) 178, 797, 881, 898
HpaI GTTAAC 1 cut(s) 1443
HpaII CCGG 3 cut(s) 233, 692, 1277
HphI GGTGA 4 cut(s) 214, 384, 415, 1377
Hpy166II GTNNAC 5 cut(s) 133, 512, 521, 1443, 1458
Hpy188I TCNGA 6 cut(s) 399, 490, 633, 889, 981, 1361
Hpy188III TCNNGA 6 cut(s) 233, 551, 679, 917, 1015, 1416
Hpy8I GTNNAC 5 cut(s) 133, 512, 521, 1443, 1458
HpyAV CCTTC 3 cut(s) 4, 429, 1413
HpyCH4III ACNGT 3 cut(s) 483, 1291, 1467
HpyCH4IV ACGT 3 cut(s) 523, 987, 1382
HpyCH4V TGCA 9 cut(s) 102, 153, 157, 245, 575, 991, 1048, 1064, 1136
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 235, 440, 1054, 1103
HpyF3I CTNAG 5 cut(s) 306, 489, 515, 978, 1410
HpySE526I ACGT 3 cut(s) 523, 987, 1382
Hsp92II CATG 8 cut(s) 157, 228, 461, 467, 778, 842, 1229, 1315
HspAI GCGC 1 cut(s) 93
Kpn2I TCCGGA 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 628
KspAI GTTAAC 1 cut(s) 1443
Kzo9I GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
LmnI GCTCC 4 cut(s) 118, 235, 493, 1157
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 1 cut(s) 1057
MaeI CTAG 4 cut(s) 126, 372, 447, 1113
MaeII ACGT 3 cut(s) 523, 987, 1382
MaeIII GTNAC 5 cut(s) 524, 833, 1330, 1348, 1383
MalI GATC 8 cut(s) 170, 208, 545, 555, 630, 915, 936, 1120
MboI GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
MboII GAAGA 8 cut(s) 56, 136, 794, 872, 932, 1003, 1386, 1395
MflI RGATCY 4 cut(s) 168, 206, 934, 1118
MluCI AATT 9 cut(s) 34, 214, 324, 331, 763, 824, 851, 1251, 1370
MlyI GAGTC 2 cut(s) 187, 791
MmeI TCCRAC 2 cut(s) 301, 962
MnlI CCTC 7 cut(s) 52, 312, 393, 405, 484, 694, 1317
Mph1103I ATGCAT 1 cut(s) 155
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 1010
MseI TTAA 5 cut(s) 33, 69, 735, 1341, 1442
MslI CAYNNNNRTG 3 cut(s) 152, 462, 968
MspI CCGG 3 cut(s) 233, 692, 1277
MspR9I CCNGG 4 cut(s) 1149, 1212, 1220, 1277
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 3 cut(s) 1149, 1212, 1220
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 5 cut(s) 99, 235, 440, 1054, 1103
NciI CCSGG 1 cut(s) 1277
NcoI CCATGG 1 cut(s) 774
NdeI CATATG 1 cut(s) 149
NdeII GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
NheI GCTAGC 1 cut(s) 125
NlaIII CATG 8 cut(s) 157, 228, 461, 467, 778, 842, 1229, 1315
NlaIV GGNNCC 4 cut(s) 170, 369, 1120, 1153
NmuCI GTSAC 2 cut(s) 1348, 1383
NsbI TGCGCA 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 155
NspI RCATGY 4 cut(s) 157, 461, 1229, 1315
PaeI GCATGC 1 cut(s) 157
PciI ACATGT 1 cut(s) 1225
PctI GAATGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 1010
PfeI GAWTC 2 cut(s) 881, 898
PflMI CCANNNNNTGG 2 cut(s) 259, 1343
PfoI TCCNGGA 1 cut(s) 1210
PinAI ACCGGT 1 cut(s) 691
PkrI GCNGC 2 cut(s) 540, 1105
PleI GAGTC 2 cut(s) 186, 791
PpsI GAGTC 2 cut(s) 186, 791
Ppu21I YACGTR 1 cut(s) 524
PscI ACATGT 1 cut(s) 1225
Psp6I CCWGG 3 cut(s) 1147, 1210, 1218
PspEI GGTNACC 1 cut(s) 833
PspGI CCWGG 3 cut(s) 1147, 1210, 1218
PspN4I GGNNCC 4 cut(s) 170, 369, 1120, 1153
PsuI RGATCY 4 cut(s) 168, 206, 934, 1118
RsaI GTAC 5 cut(s) 189, 511, 520, 1224, 1316
RsaNI GTAC 5 cut(s) 188, 510, 519, 1223, 1315
RseI CAYNNNNRTG 3 cut(s) 152, 462, 968
SaqAI TTAA 5 cut(s) 33, 69, 735, 1341, 1442
SatI GCNGC 2 cut(s) 539, 1104
Sau3AI GATC 8 cut(s) 168, 206, 543, 553, 628, 913, 934, 1118
SchI GAGTC 2 cut(s) 187, 791
ScrFI CCNGG 4 cut(s) 1149, 1212, 1220, 1277
SfaNI GCATC 1 cut(s) 1057
SmiMI CAYNNNNRTG 3 cut(s) 152, 462, 968
SmlI CTYRAG 1 cut(s) 1446
SmoI CTYRAG 1 cut(s) 1446
SpeI ACTAGT 1 cut(s) 446
SphI GCATGC 1 cut(s) 157
Sse9I AATT 9 cut(s) 34, 214, 324, 331, 763, 824, 851, 1251, 1370
SsiI CCGC 5 cut(s) 116, 617, 704, 1103, 1176
SspMI CTAG 4 cut(s) 126, 372, 447, 1113
StyD4I CCNGG 4 cut(s) 1147, 1210, 1218, 1275
StyI CCWWGG 2 cut(s) 136, 774
TaaI ACNGT 3 cut(s) 483, 1291, 1467
TaiI ACGT 3 cut(s) 526, 990, 1385
TaqI TCGA 2 cut(s) 340, 1014
TasI AATT 9 cut(s) 34, 214, 324, 331, 763, 824, 851, 1251, 1370
TatI WGTACW 3 cut(s) 509, 518, 1314
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 2 cut(s) 881, 898
Tru1I TTAA 5 cut(s) 33, 69, 735, 1341, 1442
Tru9I TTAA 5 cut(s) 33, 69, 735, 1341, 1442
TseFI GTSAC 2 cut(s) 1348, 1383
TseI GCWGC 1 cut(s) 538
Tsp45I GTSAC 2 cut(s) 1348, 1383
TspDTI ATGAA 8 cut(s) 17, 136, 325, 366, 986, 1019, 1220, 1365
TspGWI ACGGA 1 cut(s) 412
Van91I CCANNNNNTGG 2 cut(s) 259, 1343
XapI RAATTY 2 cut(s) 331, 1370
XceI RCATGY 4 cut(s) 157, 461, 1229, 1315
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 1010
XspI CTAG 4 cut(s) 126, 372, 447, 1113
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.