Rw5G005040

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002149
Physical Location & Seq
Reverse (-)
5225172 .. 5228752
3581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005040.1

Sequence Viewer

Length: 1425 bp
ATGAACCAAAAGGCTAACGTTTCTAAAGAGCTTAATGCCAAGCACAGAAAGATATTGGAAGGTCTTCTTAAATTGCCAGAGAACAGAGAATGTGCAGACTGCAAAGCCATAGCTCCACGATGGGCTAGTGTCAATCTCGGGATCTTTATATGCATGCAGTGTTCTGGGGCCCATAGAAGTCTTGGGGTACACATATCAAAGGTGAGATCTGCCACTTTGGACACATGGCTTCCAGAGCAGATTGCATTTATCCAATCGATGGGAAATGAGAAATCGAACAGTTACTGGGAAGCAGAGTTGCCTCCTAATTATGACAGAGTTGGAATCGAGAATTTCATCCGTGCAAAGTATGAATCGAAAAAATGGGTTCCTAGGGATGACAAACTAAAATCACCTCCCAGGGTGACTGCGCAGAAAACTTCAGTTTATGGAGCAAGGCCTGAAATTAGAAGCAAGCATGGCGATGTCAGAAATATTGAACAGTCTTATGAGCAGAAAGTTGCTCATCGACCTGGTAGAATAAACAGGATACCACAGCAGGTTAGTGATGATTTGAAACCGAAAAACATGGAAAAGAAATCACCAGAAGCTAGTCAAAAACCACAAGAACTTGAGCAGAAAACAGAACCAGTAGCTCCAAAACCTGAACCAGCAAGTCATAGAGCTAGTGCTACTCCAATTTCTGCACCAGTTAAAGTGGACTATGCTACTGATTTGTTCAATATGCTCTCCATGGAGGAATCAAGAGGAAGTGGCTCCGTGACATTTGCCAATAACATTACATTGGCTACTATGCATTCTGCTGAAACAAAACTGGCAGAAGAGAAAAACAATGCACCAAAATCATCTGAAAGCAAGGTGCAATCAAAATATGGTATTGAGGATTTGTTTATAGATTCTCCATCAACCACTCAAACCTTTTCAGTGAAACCTCAGACTGATGTCAAGAATGACATTATAAACTTATTTGAGAAGTATAGTTTCTCCATTCTCGATGCATCAACAACAACAACAACAACAACAATGCTCTCCCAACAACATTCATTACTAATGGCTGCTGCGCCAAATTCTAGCGGATCCCATTCCTTTTCTTCAAGTTTACAACCTGCCTCCCAAGCTGTTCACTTGCCCACACAAAATGGGCGAAGCGTTGGCCAACAAGTTCCTGGCAACCCAACGCCACCTAATCTACAATATTATAGTCAGATTGGGAATGGTTATCATGCACGTCCAATAGGCAATGCAGTTTCATACTCGACATCAAGCATGTATACGATGAGGCCGGTAGCTCCAATCAACGGTGTCAGAAGCATCGGAGGAAGCAGAGTTTCAACACCACCGCCAAAATCTTCTGTCACTCCAACTCCTTCAGGAAGGGACTTTGATTTTTCATCGTTAACTCAAGGATTGTTTACTAAACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

474

Amino Acids

52.16

Weight (kDa)

9.43

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 123 3.8e-40 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 411
Acc36I ACCTGC 2 cut(s) 529, 1114
AccB7I CCANNNNNTGG 1 cut(s) 259
AccI GTMKAC 1 cut(s) 1271
AciI CCGC 2 cut(s) 1074, 1340
AclI AACGTT 1 cut(s) 18
AclWI GGATC 3 cut(s) 149, 1071, 1084
AcoI YGGCCR 1 cut(s) 1153
AcsI RAATTY 2 cut(s) 331, 1066
AcuI CTGAAG 2 cut(s) 405, 1353
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 259, 1298
AgsI TTSAA 5 cut(s) 479, 556, 721, 1095, 1332
AjiI CACGTC 1 cut(s) 1229
AjnI CCWGG 3 cut(s) 398, 511, 1165
AluBI AGCT 7 cut(s) 31, 113, 590, 635, 665, 1118, 1289
AluI AGCT 7 cut(s) 31, 113, 590, 635, 665, 1118, 1289
AlwI GGATC 3 cut(s) 149, 1071, 1084
AlwNI CAGNNNCTG 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 4 cut(s) 168, 437, 1153, 1280
ApaI GGGCCC 1 cut(s) 172
ApeKI GCWGC 2 cut(s) 1055, 1058
ApoI RAATTY 2 cut(s) 331, 1066
Asp700I GAANNNNTTC 1 cut(s) 63
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 2 cut(s) 412, 1063
AspS9I GGNCC 2 cut(s) 168, 169
AsuHPI GGTGA 4 cut(s) 214, 384, 415, 573
AvaI CYCGRG 1 cut(s) 137
AvrII CCTAGG 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 1155
BamHI GGATCC 1 cut(s) 1076
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 1042, 1045
BccI CCATC 3 cut(s) 114, 253, 910
BciT130I CCWGG 3 cut(s) 400, 513, 1167
BciVI GTATCC 1 cut(s) 522
BfaI CTAG 5 cut(s) 126, 372, 591, 666, 1071
BfuAI ACCTGC 2 cut(s) 529, 1114
BfuI GTATCC 1 cut(s) 522
BglII AGATCT 1 cut(s) 206
BisI GCNGC 2 cut(s) 1056, 1059
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 2 cut(s) 1057, 1060
Bme1390I CCNGG 3 cut(s) 400, 513, 1167
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 1229
BmgT120I GGNCC 2 cut(s) 168, 169
BmiI GGNNCC 5 cut(s) 169, 170, 369, 757, 1078
BmrFI CCNGG 3 cut(s) 400, 513, 1167
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 3 cut(s) 985, 1007, 1320
BmuI ACTGGG 1 cut(s) 295
BoxI GACNNNNGTC 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 56
BpuEI CTTGAG 2 cut(s) 632, 1386
Bsa29I ATCGAT 1 cut(s) 257
BsaJI CCNNGG 4 cut(s) 371, 398, 399, 732
BsaXI ACNNNNNCTCC 2 cut(s) 1348, 1378
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 1298
Bse118I RCCGGY 1 cut(s) 1282
Bse1I ACTGG 4 cut(s) 290, 629, 689, 819
Bse3DI GCAATG 1 cut(s) 1246
BseBI CCWGG 3 cut(s) 400, 513, 1167
BseCI ATCGAT 1 cut(s) 257
BseDI CCNNGG 4 cut(s) 371, 398, 399, 732
BseGI GGATG 2 cut(s) 336, 382
BseLI CCNNNNNNNGG 2 cut(s) 259, 1298
BseMI GCAATG 1 cut(s) 1246
BseMII CTCAG 1 cut(s) 947
BseNI ACTGG 4 cut(s) 290, 629, 689, 819
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 2 cut(s) 1042, 1045
BsgI GTGCAG 2 cut(s) 114, 669
BshFI GGCC 4 cut(s) 170, 439, 1155, 1282
BshVI ATCGAT 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 1283
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 2 cut(s) 259, 1298
BsmFI GGGAC 1 cut(s) 1391
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 4 cut(s) 170, 439, 1155, 1282
BsoBI CYCGRG 1 cut(s) 137
Bsp120I GGGCCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 141, 206, 1076
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 2 cut(s) 1074, 1340
BspANI GGCC 4 cut(s) 170, 439, 1155, 1282
BspCNI CTCAG 1 cut(s) 946
BspDI ATCGAT 1 cut(s) 257
BspLI GGNNCC 5 cut(s) 169, 170, 369, 757, 1078
BspMI ACCTGC 2 cut(s) 529, 1114
BspPI GGATC 3 cut(s) 149, 1071, 1084
BsrDI GCAATG 1 cut(s) 1246
BsrFI RCCGGY 1 cut(s) 1282
BsrI ACTGG 4 cut(s) 290, 629, 689, 819
BssAI RCCGGY 1 cut(s) 1282
BssECI CCNNGG 4 cut(s) 371, 398, 399, 732
BssMI GATC 3 cut(s) 141, 206, 1076
BssNAI GTATAC 1 cut(s) 1272
BssT1I CCWWGG 2 cut(s) 371, 732
Bst1107I GTATAC 1 cut(s) 1272
Bst2UI CCWGG 3 cut(s) 400, 513, 1167
Bst4CI ACNGT 3 cut(s) 281, 483, 1301
Bst6I CTCTTC 1 cut(s) 816
BstC8I GCNNGC 2 cut(s) 155, 455
BstDEI CTNAG 1 cut(s) 933
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 2 cut(s) 336, 382
BstHHI GCGC 2 cut(s) 412, 1063
BstKTI GATC 3 cut(s) 144, 209, 1079
BstMBI GATC 3 cut(s) 141, 206, 1076
BstMWI GCNNNNNNNGC 3 cut(s) 235, 459, 1115
BstNI CCWGG 3 cut(s) 400, 513, 1167
BstNSI RCATGY 2 cut(s) 157, 1270
BstPAI GACNNNNGTC 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 398, 511, 1165
BstSLI GKGCMC 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 1042, 1045
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 141, 206, 1076
BstYI RGATCY 3 cut(s) 141, 206, 1076
BstZ17I GTATAC 1 cut(s) 1272
Bsu15I ATCGAT 1 cut(s) 257
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 4 cut(s) 170, 439, 1155, 1282
BsuTUI ATCGAT 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 1229
BtsCI GGATG 2 cut(s) 336, 382
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 164, 930
BveI ACCTGC 2 cut(s) 529, 1114
Cac8I GCNNGC 2 cut(s) 155, 455
CaiI CAGNNNCTG 1 cut(s) 285
CfoI GCGC 2 cut(s) 412, 1063
Cfr10I RCCGGY 1 cut(s) 1282
Cfr13I GGNCC 2 cut(s) 168, 169
ClaI ATCGAT 1 cut(s) 257
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 1 cut(s) 188
CviAII CATG 7 cut(s) 154, 225, 458, 568, 733, 1223, 1267
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 1 cut(s) 933
DpnI GATC 3 cut(s) 143, 208, 1078
DpnII GATC 3 cut(s) 141, 206, 1076
EaeI YGGCCR 1 cut(s) 1153
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
Eco130I CCWWGG 2 cut(s) 371, 732
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 405, 1353
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 168
EcoRII CCWGG 3 cut(s) 398, 511, 1165
EcoT14I CCWWGG 2 cut(s) 371, 732
EcoT22I ATGCAT 3 cut(s) 155, 798, 1000
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 371, 732
FaeI CATG 7 cut(s) 157, 228, 461, 571, 736, 1226, 1270
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 1391
FatI CATG 7 cut(s) 153, 224, 457, 567, 732, 1222, 1266
FblI GTMKAC 1 cut(s) 1271
Fnu4HI GCNGC 2 cut(s) 1056, 1059
FokI GGATG 2 cut(s) 323, 389
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 2 cut(s) 1056, 1059
FspBI CTAG 5 cut(s) 126, 372, 591, 666, 1071
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 2 cut(s) 411, 1062
GluI GCNGC 2 cut(s) 1056, 1059
HaeIII GGCC 4 cut(s) 170, 439, 1155, 1282
HapII CCGG 1 cut(s) 1283
HhaI GCGC 2 cut(s) 412, 1063
Hin1II CATG 7 cut(s) 157, 228, 461, 571, 736, 1226, 1270
Hin6I GCGC 2 cut(s) 410, 1061
HinP1I GCGC 2 cut(s) 410, 1061
HincII GTYRAC 1 cut(s) 1398
HindII GTYRAC 1 cut(s) 1398
HinfI GANTC 4 cut(s) 324, 353, 740, 896
HpaI GTTAAC 1 cut(s) 1398
HpaII CCGG 1 cut(s) 1283
HphI GGTGA 4 cut(s) 214, 384, 415, 573
Hpy166II GTNNAC 7 cut(s) 190, 700, 1100, 1123, 1272, 1398, 1413
Hpy188I TCNGA 6 cut(s) 470, 850, 936, 1206, 1307, 1316
Hpy188III TCNNGA 7 cut(s) 139, 233, 328, 744, 946, 992, 1371
Hpy8I GTNNAC 7 cut(s) 190, 700, 1100, 1123, 1272, 1398, 1413
HpyAV CCTTC 3 cut(s) 53, 1368, 1377
HpyCH4III ACNGT 3 cut(s) 281, 483, 1301
HpyCH4IV ACGT 2 cut(s) 18, 1228
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 459, 1115
HpyF3I CTNAG 1 cut(s) 933
HpySE526I ACGT 2 cut(s) 18, 1228
Hsp92II CATG 7 cut(s) 157, 228, 461, 571, 736, 1226, 1270
HspAI GCGC 2 cut(s) 410, 1061
KspAI GTTAAC 1 cut(s) 1398
Kzo9I GATC 3 cut(s) 141, 206, 1076
LmnI GCTCC 5 cut(s) 118, 431, 640, 761, 1294
Lsp1109I GCAGC 2 cut(s) 1042, 1045
LweI GCATC 3 cut(s) 985, 1007, 1320
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 5 cut(s) 126, 372, 591, 666, 1071
MaeII ACGT 2 cut(s) 18, 1228
MaeIII GTNAC 4 cut(s) 281, 403, 760, 1354
MalI GATC 3 cut(s) 143, 208, 1078
MboI GATC 3 cut(s) 141, 206, 1076
MboII GAAGA 4 cut(s) 56, 833, 1083, 1341
MflI RGATCY 3 cut(s) 141, 206, 1076
MhlI GDGCHC 1 cut(s) 172
MlsI TGGCCA 1 cut(s) 1155
MluCI AATT 6 cut(s) 71, 307, 331, 444, 678, 1066
MluNI TGGCCA 1 cut(s) 1155
MmeI TCCRAC 2 cut(s) 301, 1385
MnlI CCTC 9 cut(s) 312, 405, 730, 740, 874, 942, 1120, 1272, 1310
Mox20I TGGCCA 1 cut(s) 1155
Mph1103I ATGCAT 3 cut(s) 155, 798, 1000
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 1155
MseI TTAA 4 cut(s) 33, 69, 693, 1397
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 1155
MspI CCGG 1 cut(s) 1283
MspR9I CCNGG 3 cut(s) 400, 513, 1167
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 3 cut(s) 400, 513, 1167
MwoI GCNNNNNNNGC 3 cut(s) 235, 459, 1115
NcoI CCATGG 1 cut(s) 732
NdeII GATC 3 cut(s) 141, 206, 1076
NlaIII CATG 7 cut(s) 157, 228, 461, 571, 736, 1226, 1270
NlaIV GGNNCC 5 cut(s) 169, 170, 369, 757, 1078
NmuCI GTSAC 3 cut(s) 403, 760, 1354
NsbI TGCGCA 1 cut(s) 411
NsiI ATGCAT 3 cut(s) 155, 798, 1000
NspI RCATGY 2 cut(s) 157, 1270
PaeI GCATGC 1 cut(s) 157
PasI CCCWGGG 1 cut(s) 399
PceI AGGCCT 1 cut(s) 439
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 324, 353, 740, 896
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 2 cut(s) 1057, 1060
PshAI GACNNNNGTC 1 cut(s) 941
PsiI TTATAA 1 cut(s) 959
Psp1406I AACGTT 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 398, 511, 1165
PspGI CCWGG 3 cut(s) 398, 511, 1165
PspN4I GGNNCC 5 cut(s) 169, 170, 369, 757, 1078
PspOMI GGGCCC 1 cut(s) 168
PspPI GGNCC 2 cut(s) 168, 169
PstNI CAGNNNCTG 1 cut(s) 285
PsuI RGATCY 3 cut(s) 141, 206, 1076
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 4 cut(s) 33, 69, 693, 1397
SatI GCNGC 2 cut(s) 1056, 1059
Sau3AI GATC 3 cut(s) 141, 206, 1076
Sau96I GGNCC 2 cut(s) 168, 169
ScrFI CCNGG 3 cut(s) 400, 513, 1167
SduI GDGCHC 1 cut(s) 172
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 3 cut(s) 985, 1007, 1320
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 611, 1401
SmoI CTYRAG 2 cut(s) 611, 1401
SphI GCATGC 1 cut(s) 157
Sse9I AATT 6 cut(s) 71, 307, 331, 444, 678, 1066
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 2 cut(s) 1074, 1340
SspI AATATT 2 cut(s) 475, 1196
SspMI CTAG 5 cut(s) 126, 372, 591, 666, 1071
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 3 cut(s) 398, 511, 1165
StyI CCWWGG 2 cut(s) 371, 732
TaaI ACNGT 3 cut(s) 281, 483, 1301
TaiI ACGT 2 cut(s) 21, 1231
TaqI TCGA 7 cut(s) 257, 275, 327, 356, 508, 993, 1256
TasI AATT 6 cut(s) 71, 307, 331, 444, 678, 1066
TfiI GAWTC 4 cut(s) 324, 353, 740, 896
Tru1I TTAA 4 cut(s) 33, 69, 693, 1397
Tru9I TTAA 4 cut(s) 33, 69, 693, 1397
TscAI CASTG 2 cut(s) 164, 930
TseFI GTSAC 3 cut(s) 403, 760, 1354
TseI GCWGC 2 cut(s) 1055, 1058
Tsp45I GTSAC 3 cut(s) 403, 760, 1354
TspDTI ATGAA 6 cut(s) 17, 325, 366, 1032, 1239, 1380
TspGWI ACGGA 2 cut(s) 329, 748
TspRI CASTG 2 cut(s) 164, 930
Van91I CCANNNNNTGG 1 cut(s) 259
XapI RAATTY 2 cut(s) 331, 1066
XceI RCATGY 2 cut(s) 157, 1270
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 1163
XmaJI CCTAGG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 1271
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 5 cut(s) 126, 372, 591, 666, 1071
Zsp2I ATGCAT 3 cut(s) 155, 798, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.