Rh5BG049700

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002149
Physical Location & Seq
Reverse (-)
3944555 .. 3948752
4198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG049700.1

Sequence Viewer

Length: 1275 bp
ATGCAGTGTTCTGGGGCCCATAGAAGTCTTGGGGTACACATATCAAAGGTGAGATCTGCCACTTTGGACACATGGCTTCCAGAGCAGATTGCATTTATCCAATCTATGGGAAATGAGAAATCGAACAGTTACTGGGAAGCAGAGTTGCCTCCTAATTATGACAGAGTTGGAATCGAGAATTTCATCCGTGCAAAGTATGAATTGAAAAAATGGGTCCCCAGGGATGGCAAACTAAAATCACCTCCCAGGGTGACTGCGCAGAAAACTTCTGTTTATGGAGCAAGGCCTGAAATAAGTAGTAAGCATGGCGATGCCAGAAATATTGAACAGTCTTATGAGCAGAAAGTTGCTCATCCACCTGTTAGAATAAACAGGATACCACAGCAGGGACAGGTTAGTGATGATTTGAAACCGAAAAACATTGAAAAGAAATCACCAGAAGCTAGTCAAAAACCACGAGAACTTGAGCAGAAAACAGAACCATCAGCTCCAAAACCTGAACCAGCAAGTCATAGAAGTGCTACTCCAATTTCCGCACCAGTTAAAGTGGACTATGCTACTGATTTGTTCAATATGCTCTCCATGGAGGAATCAAGAGGAAGTGACTCCGTGACATTTGCCAATAACATTACATTGGCTACTATGCATTCTGCTGAAACAAAACTGGCAGAAGAGAAAAACAATGCACCAAAATCATCTGAAAGCAAGGTGCAATCAAAATATGGTATTGAGGATTTGTTTATAGATTCTCCATCAATCACTCAAACCTTTTCAGTGAAACCTCAGACTGATGTCAAGAATGACATTATAAACTTATTTGAGAAGTCGAGTATAGTTTCTCCATTCTCGATGCATCAACAACAACAACAAGCAATGCTCTCCCAACAACATTCATTACTAATGGCTGCTGCACCAAATTCTAGCGGATCCCATTCCTTTTCTTCAAGTTTACAACCTGCCTCCCAAGCTATTCACTTGCCCACACAAAATGGGCAAAGCGTTGGCCAACAAGTTCCTGGCAACCCAACGCCGCCTAATCTACAATACTATAGTCAGATTGGGAATGGTTATCATGCACGTCCAATAGGCAATGCAATTTCTTACTCGACATCGAGCATGTATACGATGAGGCCGGTAGCTCCAATCAACGGTGTCAGAAGCATCGGAGGAAGCAGAGTTTCAACAGCACCGCCAAAACCTTCTGTCACTCCAACTCCTTCGGGAAGGGACTTTGATTTTTCATCGTTAACTCAAGGATTGTTTACTAAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

424

Amino Acids

46.56

Weight (kDa)

9.35

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 1 - 72 5.9e-21 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
Acc16I TGCGCA 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 964
AccB7I CCANNNNNTGG 1 cut(s) 106
AccI GTMKAC 1 cut(s) 1121
AciI CCGC 4 cut(s) 534, 924, 1031, 1190
AclWI GGATC 2 cut(s) 921, 934
AcoI YGGCCR 1 cut(s) 1003
AcsI RAATTY 2 cut(s) 178, 916
AfaI GTAC 1 cut(s) 36
AfiI CCNNNNNNNGG 4 cut(s) 106, 224, 386, 1148
AgsI TTSAA 7 cut(s) 205, 326, 409, 425, 571, 945, 1182
AjiI CACGTC 1 cut(s) 1079
AjnI CCWGG 3 cut(s) 218, 245, 1015
AluBI AGCT 4 cut(s) 443, 488, 968, 1139
AluI AGCT 4 cut(s) 443, 488, 968, 1139
AlwI GGATC 2 cut(s) 921, 934
AlwNI CAGNNNCTG 1 cut(s) 132
AoxI GGCC 4 cut(s) 15, 284, 1003, 1130
ApaI GGGCCC 1 cut(s) 19
ApeKI GCWGC 2 cut(s) 905, 908
ApoI RAATTY 2 cut(s) 178, 916
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 3 cut(s) 15, 16, 214
AsuHPI GGTGA 4 cut(s) 61, 231, 262, 426
AvaII GGWCC 1 cut(s) 214
BaeGI GKGCMC 1 cut(s) 19
BalI TGGCCA 1 cut(s) 1005
BamHI GGATCC 1 cut(s) 926
BanII GRGCYC 1 cut(s) 19
BauI CACGAG 1 cut(s) 456
BbvI GCAGC 2 cut(s) 892, 895
BccI CCATC 3 cut(s) 218, 490, 760
BciT130I CCWGG 3 cut(s) 220, 247, 1017
BciVI GTATCC 1 cut(s) 369
BfaI CTAG 2 cut(s) 444, 921
BfmI CTRYAG 1 cut(s) 1048
BfuAI ACCTGC 1 cut(s) 964
BfuI GTATCC 1 cut(s) 369
BglII AGATCT 1 cut(s) 53
BisI GCNGC 3 cut(s) 906, 909, 1031
BlsI GCNGC 3 cut(s) 907, 910, 1032
Bme1390I CCNGG 3 cut(s) 220, 247, 1017
Bme18I GGWCC 1 cut(s) 214
BmgBI CACGTC 1 cut(s) 1079
BmgT120I GGNCC 3 cut(s) 15, 16, 214
BmiI GGNNCC 5 cut(s) 16, 17, 215, 216, 928
BmrFI CCNGG 3 cut(s) 220, 247, 1017
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 4 cut(s) 301, 840, 862, 1170
BmuI ACTGGG 1 cut(s) 142
BoxI GACNNNNGTC 1 cut(s) 791
BpuEI CTTGAG 2 cut(s) 485, 1236
BsaJI CCNNGG 5 cut(s) 218, 219, 245, 246, 582
BsaXI ACNNNNNCTCC 2 cut(s) 1198, 1228
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 224, 386, 1148
Bse118I RCCGGY 1 cut(s) 1132
Bse1I ACTGG 3 cut(s) 137, 539, 669
Bse3DI GCAATG 2 cut(s) 879, 1096
BseBI CCWGG 3 cut(s) 220, 247, 1017
BseDI CCNNGG 5 cut(s) 218, 219, 245, 246, 582
BseGI GGATG 3 cut(s) 183, 229, 352
BseLI CCNNNNNNNGG 4 cut(s) 106, 224, 386, 1148
BseMI GCAATG 2 cut(s) 879, 1096
BseMII CTCAG 1 cut(s) 797
BseNI ACTGG 3 cut(s) 137, 539, 669
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 2 cut(s) 892, 895
BsgI GTGCAG 1 cut(s) 894
BshFI GGCC 4 cut(s) 17, 286, 1005, 1132
BsiSI CCGG 1 cut(s) 1133
BslFI GGGAC 3 cut(s) 200, 402, 1241
BslI CCNNNNNNNGG 4 cut(s) 106, 224, 386, 1148
BsmFI GGGAC 3 cut(s) 200, 402, 1241
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 17, 286, 1005, 1132
Bsp120I GGGCCC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 2 cut(s) 53, 926
Bsp19I CCATGG 1 cut(s) 582
BspACI CCGC 4 cut(s) 534, 924, 1031, 1190
BspANI GGCC 4 cut(s) 17, 286, 1005, 1132
BspCNI CTCAG 1 cut(s) 796
BspLI GGNNCC 5 cut(s) 16, 17, 215, 216, 928
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 2 cut(s) 921, 934
BsrDI GCAATG 2 cut(s) 879, 1096
BsrFI RCCGGY 1 cut(s) 1132
BsrI ACTGG 3 cut(s) 137, 539, 669
BssAI RCCGGY 1 cut(s) 1132
BssECI CCNNGG 5 cut(s) 218, 219, 245, 246, 582
BssMI GATC 2 cut(s) 53, 926
BssNAI GTATAC 1 cut(s) 1122
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 582
Bst1107I GTATAC 1 cut(s) 1122
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 3 cut(s) 220, 247, 1017
Bst4CI ACNGT 3 cut(s) 128, 330, 1151
Bst6I CTCTTC 1 cut(s) 666
BstDEI CTNAG 1 cut(s) 783
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 183, 229, 352
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 2 cut(s) 56, 929
BstMBI GATC 2 cut(s) 53, 926
BstMWI GCNNNNNNNGC 2 cut(s) 82, 965
BstNI CCWGG 3 cut(s) 220, 247, 1017
BstNSI RCATGY 1 cut(s) 1120
BstPAI GACNNNNGTC 1 cut(s) 791
BstSCI CCNGG 3 cut(s) 218, 245, 1015
BstSFI CTRYAG 1 cut(s) 1048
BstSLI GKGCMC 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 892, 895
BstX2I RGATCY 2 cut(s) 53, 926
BstYI RGATCY 2 cut(s) 53, 926
BstZ17I GTATAC 1 cut(s) 1122
BsuI GTATCC 1 cut(s) 369
BsuRI GGCC 4 cut(s) 17, 286, 1005, 1132
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 324
BtrI CACGTC 1 cut(s) 1079
BtsCI GGATG 3 cut(s) 183, 229, 352
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 2 cut(s) 11, 780
BveI ACCTGC 1 cut(s) 964
CaiI CAGNNNCTG 1 cut(s) 132
CfoI GCGC 1 cut(s) 259
Cfr10I RCCGGY 1 cut(s) 1132
Cfr13I GGNCC 3 cut(s) 15, 16, 214
Csp6I GTAC 1 cut(s) 35
CviAII CATG 5 cut(s) 72, 305, 583, 1073, 1117
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 1 cut(s) 783
DpnI GATC 2 cut(s) 55, 928
DpnII GATC 2 cut(s) 53, 926
EaeI YGGCCR 1 cut(s) 1003
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
Eco130I CCWWGG 1 cut(s) 582
Eco147I AGGCCT 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 214
EcoO109I RGGNCCY 2 cut(s) 15, 214
EcoRII CCWGG 3 cut(s) 218, 245, 1015
EcoT14I CCWWGG 1 cut(s) 582
EcoT22I ATGCAT 2 cut(s) 648, 855
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 5 cut(s) 75, 308, 586, 1076, 1120
FaqI GGGAC 3 cut(s) 200, 402, 1241
FatI CATG 5 cut(s) 71, 304, 582, 1072, 1116
FblI GTMKAC 1 cut(s) 1121
Fnu4HI GCNGC 3 cut(s) 906, 909, 1031
FokI GGATG 3 cut(s) 170, 236, 339
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 906, 909, 1031
FspBI CTAG 2 cut(s) 444, 921
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 1 cut(s) 258
GluI GCNGC 3 cut(s) 906, 909, 1031
HaeIII GGCC 4 cut(s) 17, 286, 1005, 1132
HapII CCGG 1 cut(s) 1133
HhaI GCGC 1 cut(s) 259
Hin1II CATG 5 cut(s) 75, 308, 586, 1076, 1120
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 1 cut(s) 1248
HindII GTYRAC 1 cut(s) 1248
HinfI GANTC 4 cut(s) 171, 590, 605, 746
HpaI GTTAAC 1 cut(s) 1248
HpaII CCGG 1 cut(s) 1133
HphI GGTGA 4 cut(s) 61, 231, 262, 426
Hpy166II GTNNAC 6 cut(s) 37, 550, 950, 1122, 1248, 1263
Hpy188I TCNGA 5 cut(s) 700, 786, 1056, 1157, 1166
Hpy188III TCNNGA 6 cut(s) 80, 175, 594, 796, 847, 1221
Hpy8I GTNNAC 6 cut(s) 37, 550, 950, 1122, 1248, 1263
HpyAV CCTTC 3 cut(s) 1209, 1218, 1227
HpyCH4III ACNGT 3 cut(s) 128, 330, 1151
HpyCH4IV ACGT 1 cut(s) 1078
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 965
HpyF3I CTNAG 1 cut(s) 783
HpySE526I ACGT 1 cut(s) 1078
Hsp92II CATG 5 cut(s) 75, 308, 586, 1076, 1120
HspAI GCGC 1 cut(s) 257
KflI GGGWCCC 1 cut(s) 214
KspAI GTTAAC 1 cut(s) 1248
Kzo9I GATC 2 cut(s) 53, 926
LmnI GCTCC 3 cut(s) 278, 493, 1144
Lsp1109I GCAGC 2 cut(s) 892, 895
LweI GCATC 4 cut(s) 301, 840, 862, 1170
MaeI CTAG 2 cut(s) 444, 921
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 5 cut(s) 128, 250, 602, 610, 1204
MalI GATC 2 cut(s) 55, 928
MboI GATC 2 cut(s) 53, 926
MboII GAAGA 2 cut(s) 683, 933
MflI RGATCY 2 cut(s) 53, 926
MhlI GDGCHC 1 cut(s) 19
MlsI TGGCCA 1 cut(s) 1005
MluCI AATT 6 cut(s) 154, 178, 200, 528, 916, 1095
MluNI TGGCCA 1 cut(s) 1005
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 2 cut(s) 148, 1235
MnlI CCTC 9 cut(s) 159, 252, 580, 590, 724, 792, 970, 1122, 1160
Mox20I TGGCCA 1 cut(s) 1005
Mph1103I ATGCAT 2 cut(s) 648, 855
MscI TGGCCA 1 cut(s) 1005
MseI TTAA 2 cut(s) 543, 1247
MslI CAYNNNNRTG 2 cut(s) 309, 516
Msp20I TGGCCA 1 cut(s) 1005
MspI CCGG 1 cut(s) 1133
MspR9I CCNGG 3 cut(s) 220, 247, 1017
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 3 cut(s) 220, 247, 1017
MwoI GCNNNNNNNGC 2 cut(s) 82, 965
NcoI CCATGG 1 cut(s) 582
NdeII GATC 2 cut(s) 53, 926
NlaIII CATG 5 cut(s) 75, 308, 586, 1076, 1120
NlaIV GGNNCC 5 cut(s) 16, 17, 215, 216, 928
NmuCI GTSAC 4 cut(s) 250, 602, 610, 1204
NsbI TGCGCA 1 cut(s) 258
NsiI ATGCAT 2 cut(s) 648, 855
NspI RCATGY 1 cut(s) 1120
PasI CCCWGGG 2 cut(s) 219, 246
PceI AGGCCT 1 cut(s) 286
PctI GAATGC 1 cut(s) 646
PfeI GAWTC 3 cut(s) 171, 590, 746
PflMI CCANNNNNTGG 1 cut(s) 106
PkrI GCNGC 3 cut(s) 907, 910, 1032
PleI GAGTC 1 cut(s) 599
PpsI GAGTC 1 cut(s) 599
PpuMI RGGWCCY 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 791
PsiI TTATAA 1 cut(s) 809
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 3 cut(s) 218, 245, 1015
PspGI CCWGG 3 cut(s) 218, 245, 1015
PspN4I GGNNCC 5 cut(s) 16, 17, 215, 216, 928
PspOMI GGGCCC 1 cut(s) 15
PspPI GGNCC 3 cut(s) 15, 16, 214
PspPPI RGGWCCY 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 132
PsuI RGATCY 2 cut(s) 53, 926
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
RseI CAYNNNNRTG 2 cut(s) 309, 516
SaqAI TTAA 2 cut(s) 543, 1247
SatI GCNGC 3 cut(s) 906, 909, 1031
Sau3AI GATC 2 cut(s) 53, 926
Sau96I GGNCC 3 cut(s) 15, 16, 214
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 220, 247, 1017
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 4 cut(s) 301, 840, 862, 1170
SfcI CTRYAG 1 cut(s) 1048
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 309, 516
SmlI CTYRAG 2 cut(s) 464, 1251
SmoI CTYRAG 2 cut(s) 464, 1251
Sse9I AATT 6 cut(s) 154, 178, 200, 528, 916, 1095
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 4 cut(s) 534, 924, 1031, 1190
SspI AATATT 1 cut(s) 322
SspMI CTAG 2 cut(s) 444, 921
StuI AGGCCT 1 cut(s) 286
StyD4I CCNGG 3 cut(s) 218, 245, 1015
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 3 cut(s) 128, 330, 1151
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 6 cut(s) 122, 174, 827, 848, 1106, 1112
TasI AATT 6 cut(s) 154, 178, 200, 528, 916, 1095
TauI GCSGC 1 cut(s) 1033
TfiI GAWTC 3 cut(s) 171, 590, 746
Tru1I TTAA 2 cut(s) 543, 1247
Tru9I TTAA 2 cut(s) 543, 1247
TscAI CASTG 1 cut(s) 780
TseFI GTSAC 4 cut(s) 250, 602, 610, 1204
TseI GCWGC 2 cut(s) 905, 908
Tsp45I GTSAC 4 cut(s) 250, 602, 610, 1204
TspDTI ATGAA 4 cut(s) 172, 213, 882, 1230
TspGWI ACGGA 2 cut(s) 176, 598
TspRI CASTG 1 cut(s) 780
Van91I CCANNNNNTGG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 2 cut(s) 178, 916
XceI RCATGY 1 cut(s) 1120
XcmI CCANNNNNNNNNTGG 2 cut(s) 26, 1013
XmiI GTMKAC 1 cut(s) 1121
XspI CTAG 2 cut(s) 444, 921
Zsp2I ATGCAT 2 cut(s) 648, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.