MD05G1360900.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011999
Physical Location & Seq
Reverse (-)
47679178 .. 47681192
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1360900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGAGCTACTACAATATTTGCGCCTCCAACAAGCTTCTGAAGATGAGCTACATCATTTTATTCAACTTATTAATCATCGGCAGCAGCCTCATCACGAGATCGATTTCAATGGGGATGGCGGATATTATTGATGATCACGGAGGACAAGGAGGAGGAGGAAGAGGAGGAGGAGTGGTACAAGTACATCAGGATGAAATGAATAATAACCCGTTAGAGCAGCTGATGTCCAGCCAAGAAGATATGATGCAGGTTGCTGGATATGGAGAAGAGAAGCTTTCCACCGTCCTTATTACAGGCTCCGTCCATTGCGAGGAGGCTTGTATTGATCATAATAATTTGAATATTTTGCATCCTGATCAAACTGATCGTGATCAGCTTCATCTTCATGCATGGCCTCTATCAGGTGTTTTGGTGTCCGTCAATTGCCGTGTCAGCGGGAGAAGAAAATCCAGTTTGGCACAAGGCATCACAGACGAATTTGGGGTTTTCATGATCGATCTTCCTTCCAATCTGCATGCAATTCCCAACTTGGACAAAACGTGTTGTGTTCGAGTACTTGGAATACCAAAGAATGCACAATGCCGACCCGCTTCCGTCAGGAGGCACAAAGGACTCAAACTATCGTCCGTTGGTAATGGCATCCGAACCTACACTGCCGGCAGGATAAGGTTCCAGCATTTGACCTCTAAACCTTCACAAGCATGCGTTGAGGAAGCAAGCAACAACAATAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

246

Amino Acids

26.75

Weight (kDa)

7.65

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 128 - 197 6.3e-06 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40113 AT4G17215 AT5G47635
fragaria_vesca FvH4_3g00370
malus_domestica MD05G1360900.v1.1 MD10G1338600.v1.1
prunus_persica Prupe.4G004300_v2.0.a1
pyrus_communis pycom05g32770
rosa_chinensis RchiOBHm_Chr5g0000571
rosa_laevigata RLG00000030876
rosa_multiflora Rmu_sc0003699.1_g000003 Rmu_sc0008807.1_g000003
rosa_roxburghii Rroxscaffold_1G00075640
rosa_rugosa Rorug04G0384800
rosa_samantha Rh5AG004600 Rh5BG006300 Rh5CG005000 Rh5DG005000
rosa_wichuraiana Rw5G000460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AciI CCGC 3 cut(s) 119, 435, 588
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 3 cut(s) 177, 183, 555
AfiI CCNNNNNNNGG 3 cut(s) 310, 401, 600
AflIII ACRYGT 1 cut(s) 539
AgsI TTSAA 3 cut(s) 64, 108, 340
AluBI AGCT 6 cut(s) 6, 34, 48, 220, 274, 376
AluI AGCT 6 cut(s) 6, 34, 48, 220, 274, 376
AoxI GGCC 1 cut(s) 392
ApeKI GCWGC 3 cut(s) 81, 84, 217
ApoI RAATTY 1 cut(s) 476
AseI ATTAAT 1 cut(s) 71
AspLEI GCGC 1 cut(s) 23
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
BauI CACGAG 1 cut(s) 94
BbvI GCAGC 3 cut(s) 93, 96, 229
BccI CCATC 1 cut(s) 109
BceAI ACGGC 1 cut(s) 411
BclI TGATCA 4 cut(s) 133, 325, 355, 370
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 3 cut(s) 82, 85, 218
BlsI GCNGC 3 cut(s) 83, 86, 219
BmcAI AGTACT 1 cut(s) 555
BmiI GGNNCC 2 cut(s) 298, 671
BmsI GCATC 4 cut(s) 234, 358, 474, 648
Bsa29I ATCGAT 2 cut(s) 101, 495
BsaBI GATNNNNATC 1 cut(s) 369
BsaXI ACNNNNNCTCC 4 cut(s) 156, 159, 186, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 401, 600
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 1 cut(s) 450
Bse3DI GCAATG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 369
BseCI ATCGAT 2 cut(s) 101, 495
BseGI GGATG 4 cut(s) 120, 196, 349, 639
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 3 cut(s) 310, 401, 600
BseMI GCAATG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 6 cut(s) 165, 168, 177, 180, 183, 326
BseXI GCAGC 3 cut(s) 93, 96, 229
BshFI GGCC 1 cut(s) 394
BshVI ATCGAT 2 cut(s) 101, 495
BsiSI CCGG 1 cut(s) 657
BslI CCNNNNNNNGG 3 cut(s) 310, 401, 600
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 1 cut(s) 394
Bsp143I GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
BspACI CCGC 3 cut(s) 119, 435, 588
BspANI GGCC 1 cut(s) 394
BspDI ATCGAT 2 cut(s) 101, 495
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 2 cut(s) 298, 671
BspMI ACCTGC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 656
BssMI GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
BssSI CACGAG 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 94
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 2 cut(s) 154, 261
BstC8I GCNNGC 4 cut(s) 516, 658, 703, 718
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 4 cut(s) 120, 196, 349, 639
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 8 cut(s) 101, 136, 328, 358, 367, 373, 495, 499
BstMBI GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
BstMWI GCNNNNNNNGC 1 cut(s) 432
BstNSI RCATGY 2 cut(s) 518, 705
BstV1I GCAGC 3 cut(s) 93, 96, 229
Bsu15I ATCGAT 2 cut(s) 101, 495
BsuRI GGCC 1 cut(s) 394
BsuTUI ATCGAT 2 cut(s) 101, 495
BtsCI GGATG 4 cut(s) 120, 196, 349, 639
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 651
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 4 cut(s) 516, 658, 703, 718
CciI TCATGA 1 cut(s) 489
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 656
ClaI ATCGAT 2 cut(s) 101, 495
Csp6I GTAC 3 cut(s) 176, 182, 554
CviAII CATG 5 cut(s) 386, 390, 490, 515, 702
CviQI GTAC 3 cut(s) 176, 182, 554
DpnI GATC 8 cut(s) 100, 135, 327, 357, 366, 372, 494, 498
DpnII GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
Eam1104I CTCTTC 2 cut(s) 154, 261
EarI CTCTTC 2 cut(s) 154, 261
EciI GGCGGA 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 59
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 5 cut(s) 389, 393, 493, 518, 705
FaiI YATR 9 cut(s) 242, 261, 330, 387, 391, 491, 516, 703, 736
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FatI CATG 5 cut(s) 385, 389, 489, 514, 701
FauI CCCGC 2 cut(s) 428, 595
FbaI TGATCA 4 cut(s) 133, 325, 355, 370
Fnu4HI GCNGC 3 cut(s) 82, 85, 218
FokI GGATG 4 cut(s) 127, 203, 336, 626
Fsp4HI GCNGC 3 cut(s) 82, 85, 218
GlaI GCGC 1 cut(s) 22
GluI GCNGC 3 cut(s) 82, 85, 218
HaeIII GGCC 1 cut(s) 394
HapII CCGG 1 cut(s) 657
HhaI GCGC 1 cut(s) 23
Hin1II CATG 5 cut(s) 389, 393, 493, 518, 705
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HindIII AAGCTT 2 cut(s) 32, 272
HinfI GANTC 1 cut(s) 612
HpaII CCGG 1 cut(s) 657
Hpy188I TCNGA 2 cut(s) 39, 644
Hpy188III TCNNGA 6 cut(s) 94, 188, 353, 368, 490, 598
HpyAV CCTTC 2 cut(s) 513, 702
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 539
HpyCH4V TGCA 6 cut(s) 247, 349, 389, 514, 518, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 432
HpySE526I ACGT 1 cut(s) 539
Hsp92II CATG 5 cut(s) 389, 393, 493, 518, 705
HspAI GCGC 1 cut(s) 21
KroI GCCGGC 1 cut(s) 656
KroNI GCCGGC 1 cut(s) 658
Ksp22I TGATCA 4 cut(s) 133, 325, 355, 370
Kzo9I GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
LmnI GCTCC 1 cut(s) 302
Lsp1109I GCAGC 3 cut(s) 93, 96, 229
LweI GCATC 4 cut(s) 234, 358, 474, 648
MaeII ACGT 1 cut(s) 539
MalI GATC 8 cut(s) 100, 135, 327, 357, 366, 372, 494, 498
MboI GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
MboII GAAGA 7 cut(s) 52, 171, 248, 278, 374, 453, 491
MfeI CAATTG 1 cut(s) 421
MluCI AATT 4 cut(s) 334, 421, 476, 519
MlyI GAGTC 1 cut(s) 606
MmeI TCCRAC 1 cut(s) 51
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 656
MseI TTAA 1 cut(s) 71
MslI CAYNNNNRTG 3 cut(s) 189, 384, 700
MspA1I CMGCKG 2 cut(s) 220, 435
MspI CCGG 1 cut(s) 657
MunI CAATTG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 577
MwoI GCNNNNNNNGC 1 cut(s) 432
NaeI GCCGGC 1 cut(s) 658
NdeII GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
NgoMIV GCCGGC 1 cut(s) 656
NlaIII CATG 5 cut(s) 389, 393, 493, 518, 705
NlaIV GGNNCC 2 cut(s) 298, 671
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 518, 705
PaeI GCATGC 2 cut(s) 518, 705
PagI TCATGA 1 cut(s) 489
PctI GAATGC 1 cut(s) 577
PdiI GCCGGC 1 cut(s) 658
PkrI GCNGC 3 cut(s) 83, 86, 219
PleI GAGTC 1 cut(s) 606
PpsI GAGTC 1 cut(s) 606
PshBI ATTAAT 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 298, 671
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 3 cut(s) 177, 183, 555
RsaNI GTAC 3 cut(s) 176, 182, 554
RseI CAYNNNNRTG 3 cut(s) 189, 384, 700
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 3 cut(s) 82, 85, 218
Sau3AI GATC 8 cut(s) 98, 133, 325, 355, 364, 370, 492, 496
ScaI AGTACT 1 cut(s) 555
SchI GAGTC 1 cut(s) 606
SfaNI GCATC 4 cut(s) 234, 358, 474, 648
SmiMI CAYNNNNRTG 3 cut(s) 189, 384, 700
SphI GCATGC 2 cut(s) 518, 705
Sse9I AATT 4 cut(s) 334, 421, 476, 519
SsiI CCGC 3 cut(s) 119, 435, 588
SspI AATATT 2 cut(s) 16, 343
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 542
TaqI TCGA 3 cut(s) 101, 495, 550
TasI AATT 4 cut(s) 334, 421, 476, 519
TatI WGTACW 2 cut(s) 181, 553
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 1 cut(s) 658
TseI GCWGC 3 cut(s) 81, 84, 217
TspDTI ATGAA 5 cut(s) 207, 212, 368, 374, 478
TspGWI ACGGA 5 cut(s) 153, 289, 406, 583, 616
TspRI CASTG 1 cut(s) 658
VspI ATTAAT 1 cut(s) 71
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 1 cut(s) 476
XceI RCATGY 2 cut(s) 518, 705
ZrmI AGTACT 1 cut(s) 555
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.