MD10G1338600.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011999
Physical Location & Seq
Reverse (-)
41468708 .. 41470580
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1338600.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAGCTACTACAATATTTGCGGCTGCAACAAGCTTGTCAAGATGAGCTACATCATTTTATTCAACTTGTTGATCATCGGCAGCAGCCTCATAACGCGATCAATTTCAACGGCGATGGAGGATATTGGTGATGAAATGAATAACGACCTGTTAGAGCAGCTCATGTCCAGCCAAGAAGATATGATGCAGGTTGCTGGATATGGAGAAGAAAAGCTTTCCACCGTCCTTATTACAGGCTCAGTCCATTGCGAGGAGGCTTGTATTAATCGTAATAATTTGAATAGTTTGCATTCTGATCAAACTGATCGTGATCATCAGCTTCATCGTCATGCATGGCCTCTACCAGGAGCTTTGGTGTCTGTCAATTGCCGTGTTAGTGGGAGAAGAAGAAAATCCAGTTTGGCACAAGGCGTCACAGATGAATTCGGGGAGTTCATGATTGATCTTCCTTCCAATCTACATGCAATTCCCAACTTGGACAAAACATGTTGTGTTAGAGTACTTGGGATACCAAAGAACGCACAATGCCGACCAGCTTCTGTCAGGAGGCACAAAGGACTCAAACTATCGTCGGTTGGTAATGGCATCCGAACCTACACCGCCGGAAGGATAAGGTTCCAGCATTTGACCTCTAAACCTTCACAAGCATGCGTGGAGGAAGCAAGCAACAATCAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

227

Amino Acids

25.11

Weight (kDa)

8.08

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 106 - 179 1e-07 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40113 AT4G17215 AT5G47635
fragaria_vesca FvH4_3g00370
malus_domestica MD05G1360900.v1.1 MD10G1338600.v1.1
prunus_persica Prupe.4G004300_v2.0.a1
pyrus_communis pycom05g32770
rosa_chinensis RchiOBHm_Chr5g0000571
rosa_laevigata RLG00000030876
rosa_multiflora Rmu_sc0003699.1_g000003 Rmu_sc0008807.1_g000003
rosa_roxburghii Rroxscaffold_1G00075640
rosa_rugosa Rorug04G0384800
rosa_samantha Rh5AG004600 Rh5BG006300 Rh5CG005000 Rh5DG005000
rosa_wichuraiana Rw5G000460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 178
AccII CGCG 1 cut(s) 97
AciI CCGC 2 cut(s) 21, 600
AcsI RAATTY 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 1 cut(s) 501
AfiI CCNNNNNNNGG 3 cut(s) 250, 344, 606
AflIII ACRYGT 1 cut(s) 485
AgsI TTSAA 3 cut(s) 64, 108, 280
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 8 cut(s) 6, 34, 48, 160, 214, 319, 350, 536
AluI AGCT 8 cut(s) 6, 34, 48, 160, 214, 319, 350, 536
AlwNI CAGNNNCTG 1 cut(s) 539
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 4 cut(s) 24, 81, 84, 157
ApoI RAATTY 1 cut(s) 422
AseI ATTAAT 1 cut(s) 264
AsuHPI GGTGA 1 cut(s) 140
BbvI GCAGC 4 cut(s) 11, 93, 96, 169
BccI CCATC 1 cut(s) 109
BceAI ACGGC 2 cut(s) 126, 354
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 501
BclI TGATCA 3 cut(s) 72, 295, 310
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 5 cut(s) 22, 25, 82, 85, 158
BlsI GCNGC 5 cut(s) 23, 26, 83, 86, 159
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 1 cut(s) 345
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 2 cut(s) 174, 594
BsaBI GATNNNNATC 1 cut(s) 309
BsaHI GRCGYC 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 344, 606
Bse1I ACTGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 585
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 3 cut(s) 250, 344, 606
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 4 cut(s) 11, 93, 96, 169
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 603
BslI CCNNNNNNNGG 3 cut(s) 250, 344, 606
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 337
Bsp143I GATC 6 cut(s) 72, 98, 295, 304, 310, 442
BspACI CCGC 2 cut(s) 21, 600
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 1 cut(s) 251
BspFNI CGCG 1 cut(s) 97
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 617
BspMI ACCTGC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 244
BsrI ACTGG 1 cut(s) 396
BssMI GATC 6 cut(s) 72, 98, 295, 304, 310, 442
BssNI GRCGYC 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 223
BstACI GRCGYC 1 cut(s) 411
BstC8I GCNNGC 2 cut(s) 649, 664
BstDEI CTNAG 1 cut(s) 238
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 585
BstFNI CGCG 1 cut(s) 97
BstKTI GATC 6 cut(s) 75, 101, 298, 307, 313, 445
BstMBI GATC 6 cut(s) 72, 98, 295, 304, 310, 442
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 3 cut(s) 464, 489, 651
BstSCI CCNGG 1 cut(s) 343
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 4 cut(s) 11, 93, 96, 169
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 128
BtsCI GGATG 1 cut(s) 585
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 2 cut(s) 649, 664
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 435
CseI GACGC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 500
CviAII CATG 7 cut(s) 163, 329, 333, 436, 461, 486, 648
CviQI GTAC 1 cut(s) 500
DdeI CTNAG 1 cut(s) 238
DpnI GATC 6 cut(s) 74, 100, 297, 306, 312, 444
DpnII GATC 6 cut(s) 72, 98, 295, 304, 310, 442
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 343
EcoT22I ATGCAT 1 cut(s) 334
FaeI CATG 7 cut(s) 166, 332, 336, 439, 464, 489, 651
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FatI CATG 7 cut(s) 162, 328, 332, 435, 460, 485, 647
FbaI TGATCA 3 cut(s) 72, 295, 310
Fnu4HI GCNGC 5 cut(s) 22, 25, 82, 85, 158
FokI GGATG 1 cut(s) 572
Fsp4HI GCNGC 5 cut(s) 22, 25, 82, 85, 158
GluI GCNGC 5 cut(s) 22, 25, 82, 85, 158
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 603
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 7 cut(s) 166, 332, 336, 439, 464, 489, 651
HindIII AAGCTT 2 cut(s) 32, 212
HinfI GANTC 1 cut(s) 558
HpaII CCGG 1 cut(s) 603
HphI GGTGA 1 cut(s) 140
Hpy188I TCNGA 3 cut(s) 295, 590, 675
Hpy188III TCNNGA 4 cut(s) 40, 308, 436, 544
Hpy99I CGWCG 1 cut(s) 574
HpyAV CCTTC 3 cut(s) 459, 600, 648
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 5 cut(s) 27, 187, 289, 332, 464
HpyF3I CTNAG 1 cut(s) 238
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 7 cut(s) 166, 332, 336, 439, 464, 489, 651
Ksp22I TGATCA 3 cut(s) 72, 295, 310
Kzo9I GATC 6 cut(s) 72, 98, 295, 304, 310, 442
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 4 cut(s) 11, 93, 96, 169
LweI GCATC 2 cut(s) 174, 594
MaeIII GTNAC 1 cut(s) 412
MalI GATC 6 cut(s) 74, 100, 297, 306, 312, 444
MboI GATC 6 cut(s) 72, 98, 295, 304, 310, 442
MboII GAAGA 5 cut(s) 188, 218, 396, 399, 437
MfeI CAATTG 1 cut(s) 364
MluCI AATT 5 cut(s) 102, 274, 364, 422, 465
MlyI GAGTC 1 cut(s) 552
MnlI CCTC 8 cut(s) 98, 112, 244, 247, 348, 540, 640, 649
Mph1103I ATGCAT 1 cut(s) 334
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 2 cut(s) 327, 646
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 1 cut(s) 345
MunI CAATTG 1 cut(s) 364
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 97
NdeII GATC 6 cut(s) 72, 98, 295, 304, 310, 442
NlaIII CATG 7 cut(s) 166, 332, 336, 439, 464, 489, 651
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 1 cut(s) 412
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 3 cut(s) 464, 489, 651
PaeI GCATGC 1 cut(s) 651
PagI TCATGA 1 cut(s) 435
PciI ACATGT 1 cut(s) 485
PctI GAATGC 1 cut(s) 289
PkrI GCNGC 5 cut(s) 23, 26, 83, 86, 159
PleI GAGTC 1 cut(s) 552
PpsI GAGTC 1 cut(s) 552
PscI ACATGT 1 cut(s) 485
PshBI ATTAAT 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 617
PstNI CAGNNNCTG 1 cut(s) 539
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
RseI CAYNNNNRTG 2 cut(s) 327, 646
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 5 cut(s) 22, 25, 82, 85, 158
Sau3AI GATC 6 cut(s) 72, 98, 295, 304, 310, 442
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 1 cut(s) 552
ScrFI CCNGG 1 cut(s) 345
SfaNI GCATC 2 cut(s) 174, 594
SmiMI CAYNNNNRTG 2 cut(s) 327, 646
SphI GCATGC 1 cut(s) 651
Sse9I AATT 5 cut(s) 102, 274, 364, 422, 465
SsiI CCGC 2 cut(s) 21, 600
SspI AATATT 1 cut(s) 16
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 1 cut(s) 223
TasI AATT 5 cut(s) 102, 274, 364, 422, 465
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 24
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 4 cut(s) 24, 81, 84, 157
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 5 cut(s) 147, 152, 311, 424, 435
VspI ATTAAT 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 1 cut(s) 422
XceI RCATGY 3 cut(s) 464, 489, 651
ZrmI AGTACT 1 cut(s) 501
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.