pycom05g32770

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011999
Physical Location & Seq
Reverse (-)
32043758 .. 32045446
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32770.1

Sequence Viewer

Length: 612 bp
ATGTGGAATTTAAATGGGACCATCGCGAGGGCATTAGCTTACTATAAAACCAAAACCAAAACCAAAGGAGGAGTACTGATGATCAACTCACGTAGAGGTAACACAAAGAAGATGAGCTACTACAATATTTGCGCCTCCAACAAGCTTCTGAAGATGAGCTGCATCATTTTATTCAACTTATTAATCATCGGCAGCAGCCTCGTCACAAGATCGATTTCAATGGCGATGGCGGATATTATTGATGATCACGGAGGACAAGGAGGAGGAGGAAGGGGAGGAGGAGTGGCACAAGTACAACAGGATGAAATGAATAATAACCCGTTAGAGCAGCTGATGTCCAGCCAAGAAGATATGATGCAGGTTGCTGGATATGGAGAAGAAAAGCTTTCCACCGTCCTTATTACAGGCTCCGTCCATTGCGAGGAGGCTTGTATTAATCATAATAATTTGAATATTTTGCATCCTGATCAAACTGATCGTTGTAGATCGATCAGCTTCATCTTCATGCATGGCCTCTATCAGGTGCTTTGGTGTCCGTCAATTGCCGTGTCAGTGGGAGAAGAAAATCCAGTTTGGCACAAGGCGTCACAGACGAATTTGGGGATTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

204

Amino Acids

22.27

Weight (kDa)

7.64

Isoelectric Point (pI)

57.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40113 AT4G17215 AT5G47635
fragaria_vesca FvH4_3g00370
malus_domestica MD05G1360900.v1.1 MD10G1338600.v1.1
prunus_persica Prupe.4G004300_v2.0.a1
pyrus_communis pycom05g32770
rosa_chinensis RchiOBHm_Chr5g0000571
rosa_laevigata RLG00000030876
rosa_multiflora Rmu_sc0003699.1_g000003 Rmu_sc0008807.1_g000003
rosa_roxburghii Rroxscaffold_1G00075640
rosa_rugosa Rorug04G0384800
rosa_samantha Rh5AG004600 Rh5BG006300 Rh5CG005000 Rh5DG005000
rosa_wichuraiana Rw5G000460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccII CGCG 1 cut(s) 26
AciI CCGC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 7, 595
AcuI CTGAAG 1 cut(s) 170
AcyI GRCGYC 1 cut(s) 584
AfaI GTAC 2 cut(s) 75, 294
AfiI CCNNNNNNNGG 3 cut(s) 27, 421, 520
AgsI TTSAA 3 cut(s) 175, 219, 451
AluBI AGCT 7 cut(s) 38, 117, 145, 159, 331, 385, 495
AluI AGCT 7 cut(s) 38, 117, 145, 159, 331, 385, 495
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 4 cut(s) 159, 192, 195, 328
ApoI RAATTY 2 cut(s) 7, 595
AseI ATTAAT 2 cut(s) 182, 435
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 18
AvaII GGWCC 1 cut(s) 18
BarI GAAGNNNNNNTAC 2 cut(s) 101, 133
BbvI GCAGC 4 cut(s) 146, 204, 207, 340
BccI CCATC 2 cut(s) 29, 220
BceAI ACGGC 1 cut(s) 530
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BclI TGATCA 3 cut(s) 81, 244, 466
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 4 cut(s) 160, 193, 196, 329
BlsI GCNGC 4 cut(s) 161, 194, 197, 330
BmcAI AGTACT 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 19, 409
BmsI GCATC 3 cut(s) 171, 345, 469
Bsa29I ATCGAT 2 cut(s) 212, 488
BsaAI YACGTR 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 421, 520
Bse1I ACTGG 1 cut(s) 569
Bse3DI GCAATG 1 cut(s) 415
BseCI ATCGAT 2 cut(s) 212, 488
BseGI GGATG 2 cut(s) 307, 460
BseLI CCNNNNNNNGG 3 cut(s) 27, 421, 520
BseMI GCAATG 1 cut(s) 415
BseNI ACTGG 1 cut(s) 569
BseRI GAGGAG 6 cut(s) 84, 276, 279, 291, 294, 437
BseXI GCAGC 4 cut(s) 146, 204, 207, 340
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 513
BshVI ATCGAT 2 cut(s) 212, 488
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 3 cut(s) 27, 421, 520
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 1 cut(s) 513
Bsp143I GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
Bsp68I TCGCGA 1 cut(s) 26
BspACI CCGC 1 cut(s) 230
BspANI GGCC 1 cut(s) 513
BspDI ATCGAT 2 cut(s) 212, 488
BspFNI CGCG 1 cut(s) 26
BspHI TCATGA 1 cut(s) 608
BspLI GGNNCC 2 cut(s) 19, 409
BspMI ACCTGC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 1 cut(s) 569
BssMI GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
BssNI GRCGYC 1 cut(s) 584
Bst4CI ACNGT 1 cut(s) 394
BstACI GRCGYC 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 2 cut(s) 307, 460
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 7 cut(s) 84, 212, 247, 469, 478, 488, 492
BstMBI GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 4 cut(s) 146, 204, 207, 340
Bsu15I ATCGAT 2 cut(s) 212, 488
BsuRI GGCC 1 cut(s) 513
BsuTUI ATCGAT 2 cut(s) 212, 488
BtgZI GCGATG 2 cut(s) 7, 239
BtsCI GGATG 2 cut(s) 307, 460
BtsIMutI CAGTG 1 cut(s) 558
BtuMI TCGCGA 1 cut(s) 26
BveI ACCTGC 1 cut(s) 349
CciI TCATGA 1 cut(s) 608
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 18
ClaI ATCGAT 2 cut(s) 212, 488
CseI GACGC 1 cut(s) 573
Csp6I GTAC 2 cut(s) 74, 293
CviAII CATG 3 cut(s) 505, 509, 609
CviQI GTAC 2 cut(s) 74, 293
DpnI GATC 7 cut(s) 83, 211, 246, 468, 477, 487, 491
DpnII GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
DraI TTTAAA 1 cut(s) 12
EciI GGCGGA 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoT22I ATGCAT 1 cut(s) 510
FaeI CATG 3 cut(s) 508, 512, 612
FaiI YATR 7 cut(s) 45, 353, 372, 441, 506, 510, 610
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 1 cut(s) 31
FatI CATG 3 cut(s) 504, 508, 608
FbaI TGATCA 3 cut(s) 81, 244, 466
Fnu4HI GCNGC 4 cut(s) 160, 193, 196, 329
FokI GGATG 2 cut(s) 314, 447
Fsp4HI GCNGC 4 cut(s) 160, 193, 196, 329
GlaI GCGC 1 cut(s) 133
GluI GCNGC 4 cut(s) 160, 193, 196, 329
HaeIII GGCC 1 cut(s) 513
HgaI GACGC 1 cut(s) 573
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 3 cut(s) 508, 512, 612
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 2 cut(s) 143, 383
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 3 cut(s) 25, 464, 609
HpyAV CCTTC 1 cut(s) 264
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 4 cut(s) 162, 358, 460, 508
HpySE526I ACGT 1 cut(s) 91
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 3 cut(s) 508, 512, 612
HspAI GCGC 1 cut(s) 132
Ksp22I TGATCA 3 cut(s) 81, 244, 466
Kzo9I GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
LmnI GCTCC 1 cut(s) 413
LpnPI CCDG 8 cut(s) 284, 344, 351, 352, 390, 477, 506, 582
Lsp1109I GCAGC 4 cut(s) 146, 204, 207, 340
LweI GCATC 3 cut(s) 171, 345, 469
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 3 cut(s) 98, 202, 585
MalI GATC 7 cut(s) 83, 211, 246, 468, 477, 487, 491
MboI GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
MboII GAAGA 6 cut(s) 121, 163, 359, 389, 493, 572
MfeI CAATTG 1 cut(s) 540
MluCI AATT 4 cut(s) 7, 445, 540, 595
MmeI TCCRAC 1 cut(s) 162
Mph1103I ATGCAT 1 cut(s) 510
MseI TTAA 3 cut(s) 11, 182, 435
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 331
MunI CAATTG 1 cut(s) 540
MvnI CGCG 1 cut(s) 26
NdeII GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
NlaIII CATG 3 cut(s) 508, 512, 612
NlaIV GGNNCC 2 cut(s) 19, 409
NmuCI GTSAC 2 cut(s) 202, 585
NruI TCGCGA 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 510
PagI TCATGA 1 cut(s) 608
PkrI GCNGC 4 cut(s) 161, 194, 197, 330
Ppu21I YACGTR 1 cut(s) 92
PshBI ATTAAT 2 cut(s) 182, 435
PspN4I GGNNCC 2 cut(s) 19, 409
PspPI GGNCC 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 331
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 2 cut(s) 75, 294
RsaNI GTAC 2 cut(s) 74, 293
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 3 cut(s) 11, 182, 435
SatI GCNGC 4 cut(s) 160, 193, 196, 329
Sau3AI GATC 7 cut(s) 81, 209, 244, 466, 475, 485, 489
Sau96I GGNCC 1 cut(s) 18
ScaI AGTACT 1 cut(s) 75
SfaNI GCATC 3 cut(s) 171, 345, 469
SinI GGWCC 1 cut(s) 18
SmiI ATTTAAAT 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 4 cut(s) 7, 445, 540, 595
SsiI CCGC 1 cut(s) 230
SspI AATATT 2 cut(s) 127, 454
SwaI ATTTAAAT 1 cut(s) 12
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 94
TaqI TCGA 2 cut(s) 212, 488
TasI AATT 4 cut(s) 7, 445, 540, 595
TatI WGTACW 2 cut(s) 73, 292
Tru1I TTAA 3 cut(s) 11, 182, 435
Tru9I TTAA 3 cut(s) 11, 182, 435
TscAI CASTG 1 cut(s) 558
TseFI GTSAC 2 cut(s) 202, 585
TseI GCWGC 4 cut(s) 159, 192, 195, 328
Tsp45I GTSAC 2 cut(s) 202, 585
TspDTI ATGAA 5 cut(s) 318, 323, 487, 493, 597
TspGWI ACGGA 3 cut(s) 264, 400, 525
TspRI CASTG 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 18
VspI ATTAAT 2 cut(s) 182, 435
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 2 cut(s) 7, 595
ZrmI AGTACT 1 cut(s) 75
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.