MD06G1025300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013331
Physical Location & Seq
Reverse (-)
3069935 .. 3071145
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1025300.v1.1.491

Sequence Viewer

Length: 528 bp
ATGCAAAGACAGTCACTGGGCGGCTCGCCGTCGAAGCTCCACCAGGCCCACGGCGGACCCAAGGACCAGACTCTCACCGTCGATGACTCCCCCAACCGCATTAAGGATCTTTTAGCCTTTTCCACCACCGCCTCCTCCTCCTCCTCCTCTTCAATCTCTGCAGCCTATGAAGACGACGAGGACCATAAAGCATCGAAGCCTCACCGTCTGTCATCACCGCCGTCCATTGCACCGCACAAATCCATCCACGTCATCCCCGTCCTCACCCTCCTCTGCTTCCTCATCCTCTTCCTCTTCTCCCACGTTCCCTCCCAATCAGATTTGGCTCAGTTCAATGGCTTCACTAAACTGCCTGGATCGGCGAAGCGCGTTGTTTCTGCCGACAGCGAAATCGACGGCATCGGGCGATTTATTGACATCCGGAAGAGCGATGTTTTGGCGATCCGCAGCCTGAGGAATTTGCAGGACACTCAAACCCAAAACCTCGTCCCCAGATCTCGCTCTCACCGAAAAATCGCCGATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.11

Weight (kDa)

9.41

Isoelectric Point (pI)

59.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35470
fragaria_vesca FvH4_5g22880
malus_domestica MD06G1025300.v1.1 MD16G1281100.v1.1
prunus_persica Prupe.5G029800_v2.0.a1
pyrus_communis pycom06g02040 pycom16g25090
rosa_chinensis RchiOBHm_Chr7g0211581
rosa_laevigata RLG00000002997
rosa_multiflora Rmu_ssc0000317.1_g000007
rosa_roxburghii Rroxscaffold_3G00247720
rosa_rugosa Rorug07G0126100 Rorug07G0126100
rosa_samantha Rh7AG258400 Rh7BG252700 Rh7CG275500 Rh7DG266000
rosa_wichuraiana Rw7G022100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 369
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 7 cut(s) 21, 54, 97, 129, 218, 233, 445
AclWI GGATC 3 cut(s) 114, 364, 436
AcsI RAATTY 1 cut(s) 457
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 2 cut(s) 153, 334
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 2 cut(s) 42, 352
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
AlwI GGATC 3 cut(s) 114, 364, 436
AlwNI CAGNNNCTG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 420
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 161, 447
ApoI RAATTY 1 cut(s) 457
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 4 cut(s) 46, 56, 64, 181
AsuHPI GGTGA 5 cut(s) 67, 194, 207, 256, 497
AvaII GGWCC 3 cut(s) 56, 64, 181
AxyI CCTNAGG 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 173, 459
BccI CCATC 1 cut(s) 251
BceAI ACGGC 4 cut(s) 13, 67, 205, 412
BciT130I CCWGG 2 cut(s) 44, 354
BfmI CTRYAG 1 cut(s) 159
BglII AGATCT 1 cut(s) 494
BisI GCNGC 3 cut(s) 22, 162, 448
BlsI GCNGC 3 cut(s) 23, 163, 449
Bme1390I CCNGG 2 cut(s) 44, 354
Bme18I GGWCC 3 cut(s) 56, 64, 181
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 4 cut(s) 46, 56, 64, 181
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 2 cut(s) 44, 354
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 200, 408
BmuI ACTGGG 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 49, 60
BsaWI WCCGGW 1 cut(s) 420
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 21
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 225
BseAI TCCGGA 1 cut(s) 420
BseBI CCWGG 2 cut(s) 44, 354
BseDI CCNNGG 2 cut(s) 49, 60
BseGI GGATG 4 cut(s) 243, 252, 282, 417
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 341, 443
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 6 cut(s) 124, 127, 130, 133, 136, 260
BseXI GCAGC 2 cut(s) 173, 459
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 421
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 473
BsnI GGCC 1 cut(s) 47
Bsp13I TCCGGA 1 cut(s) 420
Bsp143I GATC 4 cut(s) 106, 356, 441, 494
BspACI CCGC 7 cut(s) 21, 54, 97, 129, 218, 233, 445
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 2 cut(s) 340, 444
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 58
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 3 cut(s) 114, 364, 436
BspQI GCTCTTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 21
BssECI CCNNGG 2 cut(s) 49, 60
BssMI GATC 4 cut(s) 106, 356, 441, 494
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 44, 354
Bst4CI ACNGT 3 cut(s) 12, 79, 206
Bst6I CTCTTC 4 cut(s) 154, 293, 299, 419
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 2 cut(s) 327, 452
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 4 cut(s) 243, 252, 282, 417
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 4 cut(s) 109, 359, 444, 497
BstMBI GATC 4 cut(s) 106, 356, 441, 494
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 2 cut(s) 44, 354
BstSCI CCNGG 2 cut(s) 42, 352
BstSFI CTRYAG 1 cut(s) 159
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 173, 459
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 2 cut(s) 106, 494
BstYI RGATCY 2 cut(s) 106, 494
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 49
BtgZI GCGATG 1 cut(s) 444
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 4 cut(s) 243, 252, 282, 417
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 26
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 1 cut(s) 369
Cfr13I GGNCC 4 cut(s) 46, 56, 64, 181
CviJI RGCY 9 cut(s) 24, 37, 47, 116, 164, 199, 326, 339, 450
CviKI_1 RGCY 9 cut(s) 24, 37, 47, 116, 164, 199, 326, 339, 450
DdeI CTNAG 2 cut(s) 327, 452
DpnI GATC 4 cut(s) 108, 358, 443, 496
DpnII GATC 4 cut(s) 106, 356, 441, 494
Eam1104I CTCTTC 4 cut(s) 154, 293, 299, 419
EarI CTCTTC 4 cut(s) 154, 293, 299, 419
EciI GGCGGA 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 60
Eco47I GGWCC 3 cut(s) 56, 64, 181
Eco81I CCTNAGG 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 42, 352
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FaiI YATR 2 cut(s) 168, 186
FaqI GGGAC 1 cut(s) 473
Fnu4HI GCNGC 3 cut(s) 22, 162, 448
FokI GGATG 4 cut(s) 230, 239, 269, 404
Fsp4HI GCNGC 3 cut(s) 22, 162, 448
GlaI GCGC 1 cut(s) 368
GluI GCNGC 3 cut(s) 22, 162, 448
HaeIII GGCC 1 cut(s) 47
HapII CCGG 1 cut(s) 421
HhaI GCGC 1 cut(s) 369
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HinfI GANTC 2 cut(s) 70, 86
HpaII CCGG 1 cut(s) 421
HphI GGTGA 5 cut(s) 67, 194, 207, 256, 497
Hpy188I TCNGA 1 cut(s) 319
Hpy188III TCNNGA 1 cut(s) 421
Hpy99I CGWCG 4 cut(s) 34, 83, 179, 398
HpyCH4III ACNGT 3 cut(s) 12, 79, 206
HpyCH4IV ACGT 2 cut(s) 249, 303
HpyCH4V TGCA 4 cut(s) 4, 161, 230, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 2 cut(s) 327, 452
HpySE526I ACGT 2 cut(s) 249, 303
HspAI GCGC 1 cut(s) 367
Kpn2I TCCGGA 1 cut(s) 420
Kzo9I GATC 4 cut(s) 106, 356, 441, 494
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 2 cut(s) 173, 459
LweI GCATC 2 cut(s) 200, 408
MaeII ACGT 2 cut(s) 249, 303
MaeIII GTNAC 1 cut(s) 12
MalI GATC 4 cut(s) 108, 358, 443, 496
MboI GATC 4 cut(s) 106, 356, 441, 494
MboII GAAGA 5 cut(s) 141, 182, 280, 286, 436
MflI RGATCY 2 cut(s) 106, 494
MluCI AATT 1 cut(s) 457
MlyI GAGTC 2 cut(s) 64, 80
MroI TCCGGA 1 cut(s) 420
MseI TTAA 2 cut(s) 102, 526
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 44, 354
MvaI CCWGG 2 cut(s) 44, 354
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 34
NdeII GATC 4 cut(s) 106, 356, 441, 494
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 505
PkrI GCNGC 3 cut(s) 23, 163, 449
PleI GAGTC 2 cut(s) 64, 80
PpsI GAGTC 2 cut(s) 64, 80
Psp6I CCWGG 2 cut(s) 42, 352
PspGI CCWGG 2 cut(s) 42, 352
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 4 cut(s) 46, 56, 64, 181
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 2 cut(s) 106, 494
SapI GCTCTTC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 102, 526
SatI GCNGC 3 cut(s) 22, 162, 448
Sau3AI GATC 4 cut(s) 106, 356, 441, 494
Sau96I GGNCC 4 cut(s) 46, 56, 64, 181
SchI GAGTC 2 cut(s) 64, 80
ScrFI CCNGG 2 cut(s) 44, 354
SetI ASST 4 cut(s) 39, 252, 306, 486
SfaNI GCATC 2 cut(s) 200, 408
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 3 cut(s) 56, 64, 181
Sse9I AATT 1 cut(s) 457
SsiI CCGC 7 cut(s) 21, 54, 97, 129, 218, 233, 445
StyD4I CCNGG 2 cut(s) 42, 352
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 3 cut(s) 12, 79, 206
TaiI ACGT 2 cut(s) 252, 306
TaqI TCGA 4 cut(s) 32, 81, 194, 393
TasI AATT 1 cut(s) 457
TauI GCSGC 1 cut(s) 24
Tru1I TTAA 2 cut(s) 102, 526
Tru9I TTAA 2 cut(s) 102, 526
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 2 cut(s) 161, 447
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 1 cut(s) 183
TspRI CASTG 1 cut(s) 21
VpaK11BI GGWCC 3 cut(s) 56, 64, 181
XapI RAATTY 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.