MD06G1117700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006894
Physical Location & Seq
Reverse (-)
25647232 .. 25649212
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1117700.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCCGCAATCCACTGCTCCTCCACTCTCCACTCTCTTAAACCGCCGCGGCCGCTTCCTCCTCAGCTCTCCAGCCTCAGACCCTCTCTCCTCTCCGCCAGCAAACACCCAAACAGTAAGAAGAACAAAAAGCTCACCGGCTACAGACCCTGCAGAGCGGAGTTCTCAAACGACGCGCCGTTCGTCGCCGCCATTGGCGCCTGCATGCTCTCGTCGCTGGTGCTTCCCGCCAGCACTCCCGATGACGATGCCGAAGGCGGTTCGGCAATGGATTCCACCGATGCCAGGTTCGCCGTCATGGGCGTCATCAGCTTCATTCCCTACTTCAATTGGCTGAGTTGGATTTTTGCTTGGTTGGATACCGGAAAACGGCGGTATGCGGTGTATGCGCTTGTGTACTTGGTTCCCTACCTGAGGACGAATTTGTCGTTGTCACCTGAGGAGAGCTGGCTGCCCATTGCTAGCATCGTTTTCTGCATTATTCATGTCCAGCTGGAAACAAGTATTAAAAATGGAGATCTTCAGGGCTTTCAATTCTTTAATGAGGCTGCAAAGCATCTCTCGTCAACAACTAGTAGGAAAGATCATCGGAGTGGGTATCAAGGAACCTCTGAGGTGGGGAAGAAAAGAGGAAATAAGAACCTTCCATCCAGTGATGAACAATCGAGAAATGAGATCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.18

Weight (kDa)

8.86

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 424
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 158
AccII CGCG 2 cut(s) 49, 176
AcoI YGGCCR 2 cut(s) 3, 50
AcsI RAATTY 1 cut(s) 421
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 2 cut(s) 198, 303
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 3 cut(s) 285, 369, 414
AgsI TTSAA 2 cut(s) 328, 533
AhlI ACTAGT 1 cut(s) 572
AjnI CCWGG 1 cut(s) 284
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
AluBI AGCT 5 cut(s) 67, 133, 312, 447, 493
AluI AGCT 5 cut(s) 67, 133, 312, 447, 493
AlwNI CAGNNNCTG 1 cut(s) 150
AoxI GGCC 2 cut(s) 3, 50
ApeKI GCWGC 2 cut(s) 451, 548
ApoI RAATTY 1 cut(s) 421
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AspLEI GCGC 3 cut(s) 178, 200, 391
AsuHPI GGTGA 2 cut(s) 127, 426
AsuNHI GCTAGC 1 cut(s) 461
AxyI CCTNAGG 2 cut(s) 413, 438
BanI GGYRCC 1 cut(s) 197
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 438, 535
BccI CCATC 1 cut(s) 655
BceAI ACGGC 3 cut(s) 163, 278, 386
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 286
BciVI GTATCC 1 cut(s) 352
BcuI ACTAGT 1 cut(s) 572
BfaI CTAG 2 cut(s) 462, 573
BfmI CTRYAG 2 cut(s) 142, 151
BfoI RGCGCY 1 cut(s) 201
BfuI GTATCC 1 cut(s) 352
BglII AGATCT 1 cut(s) 517
BisI GCNGC 7 cut(s) 6, 47, 50, 53, 189, 452, 549
BlsI GCNGC 7 cut(s) 7, 48, 51, 54, 190, 453, 550
Bme1390I CCNGG 1 cut(s) 286
BmiI GGNNCC 3 cut(s) 199, 405, 607
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 4 cut(s) 238, 271, 474, 565
BmtI GCTAGC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 63
Bsa29I ATCGAT 1 cut(s) 678
BsaHI GRCGYC 2 cut(s) 198, 303
BsaJI CCNNGG 1 cut(s) 47
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 72, 102
Bsc4I CCNNNNNNNGG 3 cut(s) 285, 369, 414
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 651
Bse21I CCTNAGG 2 cut(s) 413, 438
Bse3DI GCAATG 2 cut(s) 273, 456
BseBI CCWGG 1 cut(s) 286
BseCI ATCGAT 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 3 cut(s) 285, 369, 414
BseMI GCAATG 2 cut(s) 273, 456
BseMII CTCAG 6 cut(s) 77, 91, 326, 404, 429, 603
BseNI ACTGG 1 cut(s) 651
BseRI GAGGAG 4 cut(s) 10, 51, 80, 455
BseX3I CGGCCG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 438, 535
Bsh1236I CGCG 2 cut(s) 49, 176
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 5, 52
BshNI GGYRCC 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 678
BsiEI CGRYCG 1 cut(s) 53
BsiSI CCGG 2 cut(s) 138, 363
BslI CCNNNNNNNGG 3 cut(s) 285, 369, 414
BsnI GGCC 2 cut(s) 5, 52
Bsp143I GATC 3 cut(s) 517, 583, 675
BspANI GGCC 2 cut(s) 5, 52
BspCNI CTCAG 6 cut(s) 76, 90, 327, 405, 430, 604
BspDI ATCGAT 1 cut(s) 678
BspFNI CGCG 2 cut(s) 49, 176
BspLI GGNNCC 3 cut(s) 199, 405, 607
BspMAI CTGCAG 1 cut(s) 155
BspOI GCTAGC 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 273, 456
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 1 cut(s) 651
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 3 cut(s) 517, 583, 675
BssNI GRCGYC 2 cut(s) 198, 303
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 1 cut(s) 116
BstACI GRCGYC 2 cut(s) 198, 303
BstC8I GCNNGC 6 cut(s) 100, 202, 206, 232, 449, 463
BstDEI CTNAG 6 cut(s) 63, 77, 335, 413, 438, 612
BstDSI CCRYGG 1 cut(s) 47
BstENI CCTNNNNNAGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 647
BstFNI CGCG 2 cut(s) 49, 176
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 3 cut(s) 178, 200, 391
BstKTI GATC 3 cut(s) 520, 586, 678
BstMBI GATC 3 cut(s) 517, 583, 675
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 6 cut(s) 52, 197, 214, 290, 309, 386
BstNI CCWGG 1 cut(s) 286
BstNSI RCATGY 1 cut(s) 208
BstSCI CCNGG 1 cut(s) 284
BstSFI CTRYAG 2 cut(s) 142, 151
BstUI CGCG 2 cut(s) 49, 176
BstV1I GCAGC 2 cut(s) 438, 535
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BstZI CGGCCG 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 678
Bsu36I CCTNAGG 2 cut(s) 413, 438
BsuI GTATCC 1 cut(s) 352
BsuRI GGCC 2 cut(s) 5, 52
BsuTUI ATCGAT 1 cut(s) 678
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 1 cut(s) 647
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 2 cut(s) 13, 658
Cac8I GCNNGC 6 cut(s) 100, 202, 206, 232, 449, 463
CaiI CAGNNNCTG 1 cut(s) 150
CciNI GCGGCCGC 1 cut(s) 50
CfoI GCGC 3 cut(s) 178, 200, 391
Cfr10I RCCGGY 1 cut(s) 137
Cfr42I CCGCGG 1 cut(s) 50
ClaI ATCGAT 1 cut(s) 678
CseI GACGC 2 cut(s) 182, 292
Csp6I GTAC 1 cut(s) 397
CviAII CATG 3 cut(s) 205, 298, 485
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 6 cut(s) 63, 77, 335, 413, 438, 612
DinI GGCGCC 1 cut(s) 199
DpnI GATC 3 cut(s) 519, 585, 677
DpnII GATC 3 cut(s) 517, 583, 675
DrdI GACNNNNNNGTC 1 cut(s) 424
DseDI GACNNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 2 cut(s) 3, 50
EagI CGGCCG 1 cut(s) 50
EciI GGCGGA 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 50
Eco52I CGGCCG 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 506
Eco81I CCTNAGG 2 cut(s) 413, 438
EcoNI CCTNNNNNAGG 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 284
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
FaeI CATG 3 cut(s) 208, 301, 488
FaiI YATR 5 cut(s) 206, 299, 378, 387, 486
FatI CATG 3 cut(s) 204, 297, 484
FauI CCCGC 1 cut(s) 235
Fnu4HI GCNGC 7 cut(s) 6, 47, 50, 53, 189, 452, 549
FokI GGATG 1 cut(s) 634
Fsp4HI GCNGC 7 cut(s) 6, 47, 50, 53, 189, 452, 549
FspBI CTAG 2 cut(s) 462, 573
GlaI GCGC 3 cut(s) 177, 199, 390
GluI GCNGC 7 cut(s) 6, 47, 50, 53, 189, 452, 549
GsuI CTGGAG 1 cut(s) 55
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 2 cut(s) 5, 52
HapII CCGG 2 cut(s) 138, 363
HgaI GACGC 2 cut(s) 182, 292
HhaI GCGC 3 cut(s) 178, 200, 391
Hin1I GRCGYC 2 cut(s) 198, 303
Hin1II CATG 3 cut(s) 208, 301, 488
Hin6I GCGC 3 cut(s) 176, 198, 389
HinP1I GCGC 3 cut(s) 176, 198, 389
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 1 cut(s) 272
HpaII CCGG 2 cut(s) 138, 363
HphI GGTGA 2 cut(s) 127, 426
Hpy166II GTNNAC 2 cut(s) 397, 567
Hpy188I TCNGA 3 cut(s) 80, 591, 613
Hpy188III TCNNGA 2 cut(s) 239, 666
Hpy8I GTNNAC 2 cut(s) 397, 567
Hpy99I CGWCG 3 cut(s) 176, 188, 217
HpyAV CCTTC 2 cut(s) 248, 653
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4V TGCA 4 cut(s) 153, 204, 477, 551
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 197, 214, 290, 309, 386
HpyF3I CTNAG 6 cut(s) 63, 77, 335, 413, 438, 612
Hsp92I GRCGYC 2 cut(s) 198, 303
Hsp92II CATG 3 cut(s) 208, 301, 488
HspAI GCGC 3 cut(s) 176, 198, 389
KasI GGCGCC 1 cut(s) 197
KspI CCGCGG 1 cut(s) 50
Kzo9I GATC 3 cut(s) 517, 583, 675
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 2 cut(s) 438, 535
LweI GCATC 4 cut(s) 238, 271, 474, 565
MaeI CTAG 2 cut(s) 462, 573
MaeIII GTNAC 1 cut(s) 432
MalI GATC 3 cut(s) 519, 585, 677
MbiI CCGCTC 1 cut(s) 158
MboI GATC 3 cut(s) 517, 583, 675
MboII GAAGA 3 cut(s) 133, 512, 634
MfeI CAATTG 1 cut(s) 328
MflI RGATCY 1 cut(s) 517
MluCI AATT 3 cut(s) 328, 421, 533
Mly113I GGCGCC 1 cut(s) 198
MmeI TCCRAC 2 cut(s) 320, 336
MseI TTAA 3 cut(s) 39, 507, 540
MslI CAYNNNNRTG 1 cut(s) 591
MspA1I CMGCKG 2 cut(s) 49, 493
MspI CCGG 2 cut(s) 138, 363
MspR9I CCNGG 1 cut(s) 286
MunI CAATTG 1 cut(s) 328
MvaI CCWGG 1 cut(s) 286
MvnI CGCG 2 cut(s) 49, 176
MwoI GCNNNNNNNGC 6 cut(s) 52, 197, 214, 290, 309, 386
NarI GGCGCC 1 cut(s) 198
NdeII GATC 3 cut(s) 517, 583, 675
NheI GCTAGC 1 cut(s) 461
NlaIII CATG 3 cut(s) 208, 301, 488
NlaIV GGNNCC 3 cut(s) 199, 405, 607
NmuCI GTSAC 1 cut(s) 432
NotI GCGGCCGC 1 cut(s) 50
NspI RCATGY 1 cut(s) 208
PaeI GCATGC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 425
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 7 cut(s) 7, 48, 51, 54, 190, 453, 550
PluTI GGCGCC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 3 cut(s) 199, 405, 607
PstI CTGCAG 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 150
PsuI RGATCY 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 591
SacII CCGCGG 1 cut(s) 50
SaqAI TTAA 3 cut(s) 39, 507, 540
SatI GCNGC 7 cut(s) 6, 47, 50, 53, 189, 452, 549
Sau3AI GATC 3 cut(s) 517, 583, 675
ScrFI CCNGG 1 cut(s) 286
SfaNI GCATC 4 cut(s) 238, 271, 474, 565
SfcI CTRYAG 2 cut(s) 142, 151
SfoI GGCGCC 1 cut(s) 199
Sfr303I CCGCGG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 591
SpeI ACTAGT 1 cut(s) 572
SphI GCATGC 1 cut(s) 208
Sse9I AATT 3 cut(s) 328, 421, 533
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 2 cut(s) 462, 573
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 1 cut(s) 116
TaqI TCGA 2 cut(s) 665, 678
TasI AATT 3 cut(s) 328, 421, 533
TatI WGTACW 1 cut(s) 396
TauI GCSGC 5 cut(s) 8, 49, 52, 55, 191
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 3 cut(s) 39, 507, 540
Tru9I TTAA 3 cut(s) 39, 507, 540
TscAI CASTG 2 cut(s) 20, 658
TseFI GTSAC 1 cut(s) 432
TseI GCWGC 2 cut(s) 451, 548
Tsp45I GTSAC 1 cut(s) 432
TspDTI ATGAA 3 cut(s) 304, 473, 672
TspRI CASTG 2 cut(s) 20, 658
XagI CCTNNNNNAGG 1 cut(s) 412
XapI RAATTY 1 cut(s) 421
XceI RCATGY 1 cut(s) 208
XspI CTAG 2 cut(s) 462, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.