Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0015700

Physical Location & Seq

Genomic Coordinates Reverse (-)

26490443 .. 26491000

Gene Structure 558 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1123400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 558 bp

ATGGCATTTTGTGTTGGTATTAAGGTGTCTAAATGGTTTACGACACTGCTCGTCCTCGTCCACTGCTTTATGATCTTGTTGATAGTTGCACTTTCCTATGTAGGTCTGCTTAAACCTTCCCAAGAGATGACCGAGTTAGAAGACTATCGTCGTCGACAAACTGACAATCATTTTGTTTTGCTGGATCGTCTGTGCCCGTCGGCAGTCCCCGTGCCGATCCATGTTCTAACAGAATACATCAAGAGGAAGCTTCCGGTAGTGGAGTTTGGTCAAGTTTTCGACAAATACACAAGGCTTGAAAGTCGGGACACAGAATGCAATATATGCTTGGAGTGCATAGAAAGGAGCCATGAGGTGAGGGAACCTTGCAACTGTGATCATGTGTTTCACAGGGAGTGTCTGGATAGTTGGGTCAATCAGGGCCAGGTAACCTGCCCTTTGTGCAGATCTCTGTTGTTTCCGGCCAAGAGCGAAATAACGAGCTGCGGCGAAGGAAGTTCAGGGACGACGGAGAGGGATGAAGCTTACTTTAATAGGTTAGGATTCATCATGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

186

Amino Acids

21.27

Weight (kDa)

5.87

Isoelectric Point (pI)

57.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	105 - 149	5.3e-09	Ring finger domain
zf-C3HC4	PF00097	106 - 148	2.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	106 - 146	9.7e-06	RING-type zinc-finger
zf-rbx1	PF12678	107 - 149	1.2e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015700)

Species	Orthologous Gene IDs
malus_domestica	MD06G1122600.v1.1 MD06G1122900.v1.1 MD06G1123000.v1.1 MD06G1123400.v1.1 MD14G1139500.v1.1 MD14G1139600.v1.1 MD14G1139700.v1.1
prunus_persica	Prupe.5G134100_v2.0.a1
pyrus_communis	pycom06g11420 pycom14g11530
rosa_chinensis	RchiOBHm_Chr7g0187721
rosa_multiflora	Rmu_sc0004483.1_g000026
rosa_roxburghii	Rroxscaffold_3G00267120
rosa_samantha	Rh7BG090700 Rh7CG089700 Rh7DG091200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	440
AccI	GTMKAC	1 cut(s)	154
AciI	CCGC	1 cut(s)	486
AclWI	GGATC	2 cut(s)	192, 211
AcoI	YGGCCR	1 cut(s)	462
AgsI	TTSAA	1 cut(s)	299
AjnI	CCWGG	1 cut(s)	423
AluBI	AGCT	3 cut(s)	250, 483, 524
AluI	AGCT	3 cut(s)	250, 483, 524
AlwI	GGATC	2 cut(s)	192, 211
AoxI	GGCC	2 cut(s)	421, 462
ApeKI	GCWGC	1 cut(s)	483
ArsI	GACNNNNNNTTYG	2 cut(s)	155, 187
AspS9I	GGNCC	1 cut(s)	421
AsuHPI	GGTGA	1 cut(s)	367
BaeGI	GKGCMC	1 cut(s)	197
BbsI	GAAGAC	1 cut(s)	147
BbvI	GCAGC	1 cut(s)	470
BciT130I	CCWGG	1 cut(s)	425
BclI	TGATCA	1 cut(s)	376
BfuAI	ACCTGC	1 cut(s)	440
BglII	AGATCT	1 cut(s)	446
BisI	GCNGC	2 cut(s)	484, 487
BlsI	GCNGC	2 cut(s)	485, 488
Bme1390I	CCNGG	1 cut(s)	425
BmgT120I	GGNCC	1 cut(s)	421
BmiI	GGNNCC	2 cut(s)	347, 363
BmrFI	CCNGG	1 cut(s)	425
BoxI	GACNNNNGTC	1 cut(s)	147
BpiI	GAAGAC	1 cut(s)	147
BsaWI	WCCGGW	1 cut(s)	253
BseBI	CCWGG	1 cut(s)	425
BseGI	GGATG	1 cut(s)	523
BseSI	GKGCMC	1 cut(s)	197
BseXI	GCAGC	1 cut(s)	470
BsgI	GTGCAG	1 cut(s)	463
BshFI	GGCC	2 cut(s)	423, 464
BsiSI	CCGG	2 cut(s)	254, 461
BslFI	GGGAC	3 cut(s)	191, 320, 517
BsmFI	GGGAC	3 cut(s)	191, 320, 517
BsmI	GAATGC	1 cut(s)	320
BsnI	GGCC	2 cut(s)	423, 464
Bsp1286I	GDGCHC	1 cut(s)	197
Bsp143I	GATC	5 cut(s)	72, 184, 216, 376, 446
BspACI	CCGC	1 cut(s)	486
BspANI	GGCC	2 cut(s)	423, 464
BspLI	GGNNCC	2 cut(s)	347, 363
BspMI	ACCTGC	1 cut(s)	440
BspPI	GGATC	2 cut(s)	192, 211
BssMI	GATC	5 cut(s)	72, 184, 216, 376, 446
Bst2UI	CCWGG	1 cut(s)	425
Bst4CI	ACNGT	1 cut(s)	374
BstAPI	GCANNNNNTGC	1 cut(s)	324
BstEII	GGTNACC	1 cut(s)	427
BstF5I	GGATG	1 cut(s)	523
BstKTI	GATC	5 cut(s)	75, 187, 219, 379, 449
BstMBI	GATC	5 cut(s)	72, 184, 216, 376, 446
BstMWI	GCNNNNNNNGC	3 cut(s)	324, 333, 441
BstNI	CCWGG	1 cut(s)	425
BstPAI	GACNNNNGTC	1 cut(s)	147
BstPI	GGTNACC	1 cut(s)	427
BstSCI	CCNGG	1 cut(s)	423
BstSLI	GKGCMC	1 cut(s)	197
BstV1I	GCAGC	1 cut(s)	470
BstV2I	GAAGAC	1 cut(s)	147
BstX2I	RGATCY	1 cut(s)	446
BstYI	RGATCY	1 cut(s)	446
BsuRI	GGCC	2 cut(s)	423, 464
BtsCI	GGATG	1 cut(s)	523
BtsI	GCAGTG	2 cut(s)	44, 61
BtsIMutI	CAGTG	2 cut(s)	44, 61
BveI	ACCTGC	1 cut(s)	440
Cfr13I	GGNCC	1 cut(s)	421
CviAII	CATG	4 cut(s)	221, 350, 380, 550
CviJI	RGCY	7 cut(s)	250, 295, 348, 423, 464, 483, 524
CviKI_1	RGCY	7 cut(s)	250, 295, 348, 423, 464, 483, 524
DpnI	GATC	5 cut(s)	74, 186, 218, 378, 448
DpnII	GATC	5 cut(s)	72, 184, 216, 376, 446
EaeI	YGGCCR	1 cut(s)	462
Eco91I	GGTNACC	1 cut(s)	427
EcoO65I	GGTNACC	1 cut(s)	427
EcoRII	CCWGG	1 cut(s)	423
FaeI	CATG	4 cut(s)	224, 353, 383, 553
FaiI	YATR	9 cut(s)	71, 99, 222, 323, 325, 338, 351, 381, 551
FaqI	GGGAC	3 cut(s)	191, 320, 517
FatI	CATG	4 cut(s)	220, 349, 379, 549
FbaI	TGATCA	1 cut(s)	376
FblI	GTMKAC	1 cut(s)	154
Fnu4HI	GCNGC	2 cut(s)	484, 487
FokI	GGATG	1 cut(s)	530
Fsp4HI	GCNGC	2 cut(s)	484, 487
GluI	GCNGC	2 cut(s)	484, 487
HaeIII	GGCC	2 cut(s)	423, 464
HapII	CCGG	2 cut(s)	254, 461
Hin1II	CATG	4 cut(s)	224, 353, 383, 553
HincII	GTYRAC	1 cut(s)	155
HindII	GTYRAC	1 cut(s)	155
HindIII	AAGCTT	2 cut(s)	248, 522
HinfI	GANTC	1 cut(s)	543
HpaII	CCGG	2 cut(s)	254, 461
HphI	GGTGA	1 cut(s)	367
Hpy166II	GTNNAC	3 cut(s)	39, 61, 155
Hpy188III	TCNNGA	3 cut(s)	241, 305, 401
Hpy8I	GTNNAC	3 cut(s)	39, 61, 155
Hpy99I	CGWCG	4 cut(s)	153, 156, 202, 511
HpyAV	CCTTC	2 cut(s)	126, 485
HpyCH4III	ACNGT	1 cut(s)	374
HpyCH4V	TGCA	5 cut(s)	89, 318, 336, 369, 444
HpyF10VI	GCNNNNNNNGC	3 cut(s)	324, 333, 441
Hsp92II	CATG	4 cut(s)	224, 353, 383, 553
Ksp22I	TGATCA	1 cut(s)	376
Kzo9I	GATC	5 cut(s)	72, 184, 216, 376, 446
LmnI	GCTCC	1 cut(s)	345
Lsp1109I	GCAGC	1 cut(s)	470
MaeIII	GTNAC	1 cut(s)	427
MalI	GATC	5 cut(s)	74, 186, 218, 378, 448
MboI	GATC	5 cut(s)	72, 184, 216, 376, 446
MboII	GAAGA	1 cut(s)	152
MflI	RGATCY	1 cut(s)	446
MhlI	GDGCHC	1 cut(s)	197
MnlI	CCTC	5 cut(s)	65, 237, 346, 351, 507
MseI	TTAA	3 cut(s)	21, 111, 531
MspI	CCGG	2 cut(s)	254, 461
MspR9I	CCNGG	1 cut(s)	425
Mva1269I	GAATGC	1 cut(s)	320
MvaI	CCWGG	1 cut(s)	425
MwoI	GCNNNNNNNGC	3 cut(s)	324, 333, 441
NdeII	GATC	5 cut(s)	72, 184, 216, 376, 446
NlaIII	CATG	4 cut(s)	224, 353, 383, 553
NlaIV	GGNNCC	2 cut(s)	347, 363
PctI	GAATGC	1 cut(s)	320
PfeI	GAWTC	1 cut(s)	543
PkrI	GCNGC	2 cut(s)	485, 488
PshAI	GACNNNNGTC	1 cut(s)	147
Psp6I	CCWGG	1 cut(s)	423
PspEI	GGTNACC	1 cut(s)	427
PspGI	CCWGG	1 cut(s)	423
PspN4I	GGNNCC	2 cut(s)	347, 363
PspPI	GGNCC	1 cut(s)	421
PsuI	RGATCY	1 cut(s)	446
SalI	GTCGAC	1 cut(s)	153
SaqAI	TTAA	3 cut(s)	21, 111, 531
SatI	GCNGC	2 cut(s)	484, 487
Sau3AI	GATC	5 cut(s)	72, 184, 216, 376, 446
Sau96I	GGNCC	1 cut(s)	421
ScrFI	CCNGG	1 cut(s)	425
SduI	GDGCHC	1 cut(s)	197
SsiI	CCGC	1 cut(s)	486
StyD4I	CCNGG	1 cut(s)	423
TaaI	ACNGT	1 cut(s)	374
TaqI	TCGA	2 cut(s)	154, 279
TaqII	GACCGA	1 cut(s)	146
TauI	GCSGC	1 cut(s)	489
TfiI	GAWTC	1 cut(s)	543
Tru1I	TTAA	3 cut(s)	21, 111, 531
Tru9I	TTAA	3 cut(s)	21, 111, 531
TscAI	CASTG	2 cut(s)	51, 68
TseI	GCWGC	1 cut(s)	483
TspDTI	ATGAA	2 cut(s)	534, 535
TspGWI	ACGGA	1 cut(s)	524
TspRI	CASTG	2 cut(s)	51, 68
XmiI	GTMKAC	1 cut(s)	154

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.