MD14G1139700.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015700
Physical Location & Seq
Reverse (-)
23021194 .. 23021736
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1139700.v1.1.491

Sequence Viewer

Length: 543 bp
ATGCAGAAATGGTCATGCAAAACCTTAACGACAGTGTTAGTCCTTGTACACTGCATCAAGATCTTGGTGATAGTGGCACTTAGCCATCTGGGAATTCTCCAACCTTTCCAACTGATGACTGAACCAACTACTGACTCCATGGAAAATCATCCCACCAATTCAATACTTGTGTTCGACCGTTTGTACCCGTCGATACCCCCGGTGCCTCTCCATATACTAACAGCATGTATCAAGAGGCTTCCAGTGGTAGAGTTTGGTCAAGTTTTTGAAAAAATCACAAAGCATGAAACTCAGGAGACAGTTTGCAGCATATGCTTGGAGTGCATAGAGAGAAGCCATGAGGTGAGGGAACAGTGCAACTGTGACCATGTGTTTCACAGGGAGTGCCTAGATAGTTGGGTCAATCAAGGGGAAGTGACCTGCCCTTTGTGCAGAGCTCTGTTGTTTCCAGCCAAGAGAGAAACGACGAGCTGCGGTGGAGAAAATTCAGGGACGATGGAGAGGGATGGTTACCTTAATAGGTTAGAATTCATCGTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

181

Amino Acids

20.54

Weight (kDa)

6.05

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 101 - 145 2.9e-09 Ring finger domain
zf-rbx1 PF12678 101 - 145 1e-06 RING-H2 zinc finger domain
zf-C3HC4 PF00097 102 - 144 1.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 202
AciI CCGC 1 cut(s) 474
AcsI RAATTY 3 cut(s) 93, 484, 527
AfaI GTAC 2 cut(s) 48, 185
AgsI TTSAA 2 cut(s) 162, 269
AluBI AGCT 2 cut(s) 437, 471
AluI AGCT 2 cut(s) 437, 471
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 1 cut(s) 290
ApeKI GCWGC 2 cut(s) 306, 471
ApoI RAATTY 3 cut(s) 93, 484, 527
ArsI GACNNNNNNTTYG 2 cut(s) 409, 441
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 79, 355
BanI GGYRCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 439
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 2 cut(s) 318, 458
BccI CCATC 3 cut(s) 93, 490, 500
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 290
BfaI CTAG 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 428
BglII AGATCT 1 cut(s) 60
BisI GCNGC 2 cut(s) 307, 472
BlsI GCNGC 2 cut(s) 308, 473
Bme1390I CCNGG 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 138, 198
Bse1I ACTGG 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 138, 198
BseGI GGATG 2 cut(s) 148, 511
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 318, 458
BsgI GTGCAG 1 cut(s) 451
Bsh1285I CGRYCG 1 cut(s) 178
BshNI GGYRCC 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 505
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 505
Bsp1286I GDGCHC 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 46
Bsp143I GATC 1 cut(s) 60
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 202
BsrGI TGTACA 1 cut(s) 46
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 138, 198
BssMI GATC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 138
Bst4CI ACNGT 5 cut(s) 34, 179, 301, 354, 362
BstAPI GCANNNNNTGC 1 cut(s) 312
BstAUI TGTACA 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 80, 291
BstDSI CCRYGG 1 cut(s) 138
BstEII GGTNACC 1 cut(s) 509
BstF5I GGATG 2 cut(s) 148, 511
BstKTI GATC 1 cut(s) 63
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 1 cut(s) 60
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 3 cut(s) 312, 321, 429
BstNSI RCATGY 1 cut(s) 228
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 318, 458
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 148, 511
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 4 cut(s) 39, 49, 249, 359
BveI ACCTGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 47, 184
CviAII CATG 6 cut(s) 15, 139, 225, 284, 338, 368
CviJI RGCY 6 cut(s) 84, 238, 336, 437, 452, 471
CviKI_1 RGCY 6 cut(s) 84, 238, 336, 437, 452, 471
CviQI GTAC 2 cut(s) 47, 184
DdeI CTNAG 2 cut(s) 80, 291
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 138
Eco24I GRGCYC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 437
EcoO65I GGTNACC 1 cut(s) 509
EcoRI GAATTC 2 cut(s) 93, 527
EcoT14I CCWWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 6 cut(s) 18, 142, 228, 287, 341, 371
FaqI GGGAC 1 cut(s) 505
FatI CATG 6 cut(s) 14, 138, 224, 283, 337, 367
FauNDI CATATG 1 cut(s) 311
Fnu4HI GCNGC 2 cut(s) 307, 472
FokI GGATG 2 cut(s) 135, 518
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 2 cut(s) 307, 472
FspBI CTAG 1 cut(s) 389
GluI GCNGC 2 cut(s) 307, 472
HapII CCGG 1 cut(s) 200
Hin1II CATG 6 cut(s) 18, 142, 228, 287, 341, 371
HinfI GANTC 1 cut(s) 134
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 79, 355
Hpy166II GTNNAC 1 cut(s) 49
Hpy188III TCNNGA 4 cut(s) 58, 232, 293, 535
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 2 cut(s) 193, 469
HpyCH4III ACNGT 5 cut(s) 34, 179, 301, 354, 362
HpyCH4V TGCA 7 cut(s) 4, 18, 54, 306, 324, 357, 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 312, 321, 429
HpyF3I CTNAG 2 cut(s) 80, 291
Hsp92II CATG 6 cut(s) 18, 142, 228, 287, 341, 371
Kzo9I GATC 1 cut(s) 60
LpnPI CCDG 8 cut(s) 74, 213, 255, 278, 364, 433, 462, 474
Lsp1109I GCAGC 2 cut(s) 318, 458
LweI GCATC 1 cut(s) 63
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 3 cut(s) 362, 415, 509
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 4 cut(s) 93, 157, 484, 527
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 2 cut(s) 124, 133
MnlI CCTC 5 cut(s) 216, 228, 334, 339, 495
MseI TTAA 2 cut(s) 26, 516
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 312, 321, 429
NciI CCSGG 1 cut(s) 200
NcoI CCATGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 311
NdeII GATC 1 cut(s) 60
NlaIII CATG 6 cut(s) 18, 142, 228, 287, 341, 371
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 362, 415
NspI RCATGY 1 cut(s) 228
PkrI GCNGC 2 cut(s) 308, 473
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 439
PspEI GGTNACC 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 204
PsuI RGATCY 1 cut(s) 60
RsaI GTAC 2 cut(s) 48, 185
RsaNI GTAC 2 cut(s) 47, 184
SacI GAGCTC 1 cut(s) 439
SaqAI TTAA 2 cut(s) 26, 516
SatI GCNGC 2 cut(s) 307, 472
Sau3AI GATC 1 cut(s) 60
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 439
SetI ASST 8 cut(s) 26, 106, 345, 422, 439, 473, 516, 524
SfaNI GCATC 1 cut(s) 63
Sse9I AATT 4 cut(s) 93, 157, 484, 527
SsiI CCGC 1 cut(s) 474
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 439
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 5 cut(s) 34, 179, 301, 354, 362
TaqI TCGA 2 cut(s) 174, 191
TasI AATT 4 cut(s) 93, 157, 484, 527
TatI WGTACW 1 cut(s) 46
Tru1I TTAA 2 cut(s) 26, 516
Tru9I TTAA 2 cut(s) 26, 516
TscAI CASTG 4 cut(s) 39, 56, 249, 359
TseFI GTSAC 2 cut(s) 362, 415
TseI GCWGC 2 cut(s) 306, 471
Tsp45I GTSAC 2 cut(s) 362, 415
TspDTI ATGAA 2 cut(s) 300, 520
TspRI CASTG 4 cut(s) 39, 56, 249, 359
XapI RAATTY 3 cut(s) 93, 484, 527
XceI RCATGY 1 cut(s) 228
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.