MD14G1139600.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015700
Physical Location & Seq
Reverse (-)
23008699 .. 23009256
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1139600.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGAGTCCCTCTCTGTTGGTATTAAGATGCCCAAATGGCGCAAAACCCTAAAAACACTGCTAGTCCTAGTACATTGCTTCAAGATCTTGGTGGTAGTGGCACTTATCCATCTAGGGTTCCTGAAACCTCCGCAACAAATGACTCAGTCCATGGACGAGCAGAATCGCCAAAACCCTAATGGTCATATTCTCATGTTCGACCGTCTGTGCCGGTCGATGGTCCCGGTTCCGATCCACGTTTTAACAGCGTATATCAAGAGTCTTCCAGTAGTGGAGTTTGGTCGTGTTTTCGAAAAATACACAAAGCAAGAAGGTCAGGTGACAGAATGCAGTATATGCTTGGAGTGCATTGAGAGAAGTCATGAGGTGAGGGAACAGTGCAACTGTGATCATTTGTTTCACAGGGAGTGTTTAGATGGTTGGGTGAATCAAGGGCAGGTGACGTGCCCTTTGTGCAGAGCTGTGCTGTTTCCTGCCACTAGTGAAAAGACGAGCTGCGGCGGAGGTCATGCATGGACGGTAGACAGGGATGCTTACTTTGAACCTTTGATAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

186

Amino Acids

21.1

Weight (kDa)

7.56

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 109 - 153 1.3e-09 Ring finger domain
zf-C3HC4 PF00097 110 - 152 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 112 - 153 2e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
Acc36I ACCTGC 1 cut(s) 427
AccI GTMKAC 1 cut(s) 522
AciI CCGC 3 cut(s) 131, 498, 501
AclWI GGATC 1 cut(s) 226
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 217, 551
AgsI TTSAA 2 cut(s) 82, 542
AhlI ACTAGT 1 cut(s) 479
AjiI CACGTC 1 cut(s) 444
AluBI AGCT 2 cut(s) 461, 495
AluI AGCT 2 cut(s) 461, 495
AlwI GGATC 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 495
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 220
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 4 cut(s) 331, 379, 436, 451
AsuII TTCGAA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 220
BaeGI GKGCMC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 482
BccI CCATC 3 cut(s) 117, 211, 410
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 1 cut(s) 224
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 4 cut(s) 62, 68, 113, 480
BfuAI ACCTGC 1 cut(s) 427
BglI GCCNNNNNGGC 1 cut(s) 37
BglII AGATCT 1 cut(s) 84
BisI GCNGC 2 cut(s) 496, 499
BlsI GCNGC 2 cut(s) 497, 500
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 220
BmgBI CACGTC 1 cut(s) 444
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 3 cut(s) 119, 222, 228
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 2 cut(s) 18, 520
BpiI GAAGAC 1 cut(s) 254
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 551
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 2 cut(s) 217, 551
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 449
BseXI GCAGC 1 cut(s) 482
BsgI GTGCAG 1 cut(s) 475
Bsh1285I CGRYCG 2 cut(s) 202, 215
BsiEI CGRYCG 2 cut(s) 202, 215
BsiSI CCGG 2 cut(s) 211, 224
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 217, 551
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 332
Bsp119I TTCGAA 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 3 cut(s) 84, 231, 388
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 3 cut(s) 131, 498, 501
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 3 cut(s) 119, 222, 228
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 1 cut(s) 226
BspT104I TTCGAA 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 3 cut(s) 84, 231, 388
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 4 cut(s) 203, 378, 386, 520
BstAPI GCANNNNNTGC 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 144
BstDSI CCRYGG 1 cut(s) 150
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 1 cut(s) 535
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 3 cut(s) 87, 234, 391
BstMBI GATC 3 cut(s) 84, 231, 388
BstMCI CGRYCG 2 cut(s) 202, 215
BstMWI GCNNNNNNNGC 4 cut(s) 37, 336, 345, 453
BstSCI CCNGG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 449
BstV1I GCAGC 1 cut(s) 482
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 150
BtrI CACGTC 1 cut(s) 444
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 2 cut(s) 56, 383
BveI ACCTGC 1 cut(s) 427
CciI TCATGA 1 cut(s) 361
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 71
CviAII CATG 5 cut(s) 151, 193, 362, 509, 513
CviJI RGCY 2 cut(s) 461, 495
CviKI_1 RGCY 2 cut(s) 461, 495
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 1 cut(s) 144
DpnI GATC 3 cut(s) 86, 233, 390
DpnII GATC 3 cut(s) 84, 231, 388
EciI GGCGGA 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 220
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 5 cut(s) 154, 196, 365, 512, 516
FaiI YATR 9 cut(s) 152, 186, 194, 252, 335, 337, 363, 510, 514
FaqI GGGAC 1 cut(s) 206
FatI CATG 5 cut(s) 150, 192, 361, 508, 512
FbaI TGATCA 1 cut(s) 388
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 496, 499
FokI GGATG 1 cut(s) 542
Fsp4HI GCNGC 2 cut(s) 496, 499
FspBI CTAG 4 cut(s) 62, 68, 113, 480
GlaI GCGC 1 cut(s) 41
GluI GCNGC 2 cut(s) 496, 499
HapII CCGG 2 cut(s) 211, 224
HhaI GCGC 1 cut(s) 42
Hin1II CATG 5 cut(s) 154, 196, 365, 512, 516
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 5 cut(s) 5, 142, 163, 259, 427
HpaII CCGG 2 cut(s) 211, 224
HphI GGTGA 4 cut(s) 331, 379, 436, 451
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 4 cut(s) 82, 121, 256, 362
Hpy8I GTNNAC 1 cut(s) 523
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 4 cut(s) 203, 378, 386, 520
HpyCH4IV ACGT 2 cut(s) 237, 443
HpyCH4V TGCA 5 cut(s) 330, 348, 381, 456, 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 336, 345, 453
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 2 cut(s) 237, 443
Hsp92II CATG 5 cut(s) 154, 196, 365, 512, 516
HspAI GCGC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 3 cut(s) 84, 231, 388
LpnPI CCDG 9 cut(s) 134, 224, 237, 279, 302, 388, 422, 486, 511
Lsp1109I GCAGC 1 cut(s) 482
LweI GCATC 2 cut(s) 18, 520
MaeI CTAG 4 cut(s) 62, 68, 113, 480
MaeII ACGT 2 cut(s) 237, 443
MaeIII GTNAC 2 cut(s) 319, 439
MalI GATC 3 cut(s) 86, 233, 390
MboI GATC 3 cut(s) 84, 231, 388
MboII GAAGA 1 cut(s) 254
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 449
MlyI GAGTC 3 cut(s) 14, 136, 268
MnlI CCTC 5 cut(s) 20, 138, 358, 363, 497
Mph1103I ATGCAT 1 cut(s) 514
MseI TTAA 2 cut(s) 24, 242
MspI CCGG 2 cut(s) 211, 224
MspR9I CCNGG 1 cut(s) 224
Mva1269I GAATGC 1 cut(s) 332
MwoI GCNNNNNNNGC 4 cut(s) 37, 336, 345, 453
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 150
NdeII GATC 3 cut(s) 84, 231, 388
NlaIII CATG 5 cut(s) 154, 196, 365, 512, 516
NlaIV GGNNCC 3 cut(s) 119, 222, 228
NmuCI GTSAC 2 cut(s) 319, 439
NsiI ATGCAT 1 cut(s) 514
NspV TTCGAA 1 cut(s) 291
PagI TCATGA 1 cut(s) 361
PaqCI CACCTGC 1 cut(s) 427
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 2 cut(s) 163, 427
PflFI GACNNNGTC 1 cut(s) 145
PkrI GCNGC 2 cut(s) 497, 500
PleI GAGTC 3 cut(s) 13, 136, 267
PpsI GAGTC 3 cut(s) 13, 136, 267
PspN4I GGNNCC 3 cut(s) 119, 222, 228
PspPI GGNCC 1 cut(s) 220
PsuI RGATCY 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 145
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 24, 242
SatI GCNGC 2 cut(s) 496, 499
Sau3AI GATC 3 cut(s) 84, 231, 388
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 3 cut(s) 14, 136, 268
ScrFI CCNGG 1 cut(s) 224
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 2 cut(s) 18, 520
SfuI TTCGAA 1 cut(s) 291
SinI GGWCC 1 cut(s) 220
SpeI ACTAGT 1 cut(s) 479
SsiI CCGC 3 cut(s) 131, 498, 501
SspMI CTAG 4 cut(s) 62, 68, 113, 480
StyD4I CCNGG 1 cut(s) 222
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 203, 378, 386, 520
TaiI ACGT 2 cut(s) 240, 446
TaqI TCGA 3 cut(s) 198, 215, 291
TatI WGTACW 1 cut(s) 70
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 2 cut(s) 163, 427
Tru1I TTAA 2 cut(s) 24, 242
Tru9I TTAA 2 cut(s) 24, 242
TscAI CASTG 2 cut(s) 63, 383
TseFI GTSAC 2 cut(s) 319, 439
TseI GCWGC 1 cut(s) 495
Tsp45I GTSAC 2 cut(s) 319, 439
TspRI CASTG 2 cut(s) 63, 383
Tth111I GACNNNGTC 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 220
XagI CCTNNNNNAGG 1 cut(s) 549
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 4 cut(s) 62, 68, 113, 480
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.