Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0008157

Physical Location & Seq

Genomic Coordinates Forward (+)

27983353 .. 27985065

Gene Structure 1713 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1135000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGGCAAGAATATTCATGAATATAAGAAGGAGCTCAAGAGCATGGGGGTCGTTGTGGAATTCAAGCAGCATTTCCCCAGACAATGCGCTAGCATTGCTGGATTGCATGCAGATTTTATTGCAGGCGAAAGATTATTCCTTTCCGGATGCATTCAGAAAGAAAGTTTCTCAACCATGGTTGAAGACTTATGCTGGTTATAGGCCTCCCAGCAAGTGCTTGTTGTTCGACTACAAATTTGATGGGTTTCTGAAAAAGACCGACGGGCCTTTCATTGACGAAGAATTTTATGGTTCTAAAATTACAACCTACAGAAAAGAGCTCTCTGCGATAGGGGTGATCGTTGAAGTGGAGCAAGGATGTCCATTGATTGCCAGCCACCTTGATTTTCATAATGAACTTTCCACTTTCGTTCGAGTATACAAGTACTTGAATGAGTTTAAGTGGAAGCCGGATTGTGAGGCTTACAAAAGGATCTGGATTCCGAATGGAAATCAGAATGGAGAGTGGGCTAGTCCTGATCAATGCGTAATTAATGACAAAGACGGGCTATTTGGTTTGCAGCTTACTGTCCTGGAGACCTACTTTGAGCATAACCTGCTAGTGTTCTTTTCGTATGCTTTTGGGGTAAAATCTCATCCTTCTATTGAGGACTACTGCAAGCTCTGGAAGGTTTGGGAAAGCTCCAAAATAAGATTGTCGCATGCGCAGTGTTGCAAGTTCTGGGGTTATATTGCAAAGAGCTGGAATTCGAAAACAGAGAAAGTTCTTACTGAGGCCTTGGTGAAACTGCCTGTTAATTCCGGCTCAGATGAAATCTTGTTGCTAAACAAGTCGGACGTGTTTCTTGCTAATGACTTGCTACTGAAGGATCTTTTTGAGCAGTTTTCTCATCACCCCTTATTTGTTTGGTACCCGCAGCCGAGCTTGCCTGCATTACCTCGGGCTACATTGCTTGAAATTTACAAGAATATTGGTGTTTGCACTATTTCAGAATCTGTACAGAAGGAAGAACTGCCCCTAGAAATTGTCTTTGGGCAAAAGGTAATTCCAAGGGATGGTTTGATTTGGAAACCGCTGTTGAAGCTCATTCTTGGTTTTCTAGCTGATCCCGCCTTCAAAATGGAAGCAGAAAGGAGGCATGAAGCTGTGCAAGGCCTTCTCAATCTTACTATTGTGGAGACAATCGAACCAATCAATGTAAGCTATAACTTACCATTGTCTTCCGGGGAGGTTTTGAACGTGAGGGCAAGCCGAAAGATACGATGGGACAAAGAAAAGTCCACATTTTTCACACAGAAAATGGACAGATCTGGAGGGCAGAAAAGTGTCATTGAGTTTGCCACATATTTCTCTGAAGTAATATCTACCGGTGTCCTGTGGGAGAACACTGATCATATACCTGCACTTTCGGAGCTGATCAAGTTGGCATTCGTGCTGGATTTCGACGAGGAAGCAGTTGAGTTCTATATGAAGTCCAAGAATTTGCGAATCTTCGTCGAGGATGAGGAATTCCTCTGCGAACGCTGCGGCATGTTCGCTGTCCCCAGTGACCACGCGGCTGCGCGTGTTTCCTGGCGCGTGACAGCGAGTGACGGTGCGTGGCACCACCGCCGGAGATCACGGCTGCATGTGGCTGGAGCACAGAGGCGCGTGGCTGGAGCACGGGGACACATGACGGCTCGTGCGGCGGCGAGTGCAAGACCCGAGACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

571

Amino Acids

65.3

Weight (kDa)

7.91

Isoelectric Point (pI)

46.21

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008157)

Species	Orthologous Gene IDs
malus_domestica	MD06G1135000.v1.1
pyrus_communis	pycom06g12600 pycom06g12660
rosa_chinensis	RchiOBHm_Chr7g0182801 RchiOBHm_Chr7g0189211
rosa_multiflora	Rmu_sc0000239.1_g000045
rosa_roxburghii	Rroxscaffold_172G00445440 Rroxscaffold_2G00103850 Rroxscaffold_2G00125400 Rroxscaffold_2G00129600 Rroxscaffold_3G00220580 Rroxscaffold_3G00234810 Rroxscaffold_3G00243090 Rroxscaffold_3G00265650 Rroxscaffold_4G00330820 Rroxscaffold_6G00425560 Rroxscaffold_7G00169720 Rroxscaffold_7G00190290 Rroxscaffold_7G00204940 Rroxscaffold_7G00208230
rosa_samantha	Rh4BG005500 Rh4BG099100 Rh6BG169400 Rh7CG152700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	705
Acc36I	ACCTGC	2 cut(s)	603, 1408
Acc65I	GGTACC	1 cut(s)	909
AccB1I	GGYRCC	2 cut(s)	909, 1602
AccB7I	CCANNNNNTGG	1 cut(s)	1055
AccI	GTMKAC	1 cut(s)	417
AccII	CGCG	4 cut(s)	1556, 1564, 1578, 1650
AccIII	TCCGGA	1 cut(s)	142
AciI	CCGC	8 cut(s)	914, 1073, 1110, 1527, 1556, 1609, 1685, 1688
AclWI	GGATC	3 cut(s)	479, 876, 1100
AcsI	RAATTY	7 cut(s)	58, 233, 281, 745, 957, 1480, 1508
AcuI	CTGAAG	2 cut(s)	884, 1374
AfaI	GTAC	3 cut(s)	425, 911, 999
AfiI	CCNNNNNNNGG	1 cut(s)	1055
AflIII	ACRYGT	1 cut(s)	837
AgeI	ACCGGT	1 cut(s)	1367
AgsI	TTSAA	8 cut(s)	63, 181, 344, 430, 956, 1081, 1117, 1237
AjiI	CACGTC	1 cut(s)	838
AjnI	CCWGG	2 cut(s)	570, 1571
AjuI	GAANNNNNNNTTGG	2 cut(s)	1014, 1046
Alw21I	GWGCWC	4 cut(s)	35, 321, 1642, 1663
Alw26I	GTCTC	3 cut(s)	569, 1172, 1700
AlwI	GGATC	3 cut(s)	479, 876, 1100
AlwNI	CAGNNNCTG	1 cut(s)	995
Ama87I	CYCGRG	2 cut(s)	939, 1703
Aor13HI	TCCGGA	1 cut(s)	142
AoxI	GGCC	4 cut(s)	200, 263, 774, 1153
ApeKI	GCWGC	6 cut(s)	66, 559, 916, 1524, 1559, 1624
ApoI	RAATTY	7 cut(s)	58, 233, 281, 745, 957, 1480, 1508
ArsI	GACNNNNNNTTYG	4 cut(s)	218, 250, 533, 565
AseI	ATTAAT	1 cut(s)	531
AsiGI	ACCGGT	1 cut(s)	1367
Asp718I	GGTACC	1 cut(s)	909
AspLEI	GCGC	5 cut(s)	88, 706, 1564, 1578, 1650
AspS9I	GGNCC	1 cut(s)	263
AsuC2I	CCSGG	1 cut(s)	1225
AsuHPI	GGTGA	3 cut(s)	346, 793, 884
AsuII	TTCGAA	1 cut(s)	749
AsuNHI	GCTAGC	1 cut(s)	88
AvaI	CYCGRG	2 cut(s)	939, 1703
BanI	GGYRCC	2 cut(s)	909, 1602
BanII	GRGCYC	2 cut(s)	35, 321
BauI	CACGAG	1 cut(s)	1680
BbsI	GAAGAC	2 cut(s)	188, 1212
Bbv12I	GWGCWC	4 cut(s)	35, 321, 1642, 1663
BbvI	GCAGC	6 cut(s)	78, 571, 928, 1511, 1546, 1611
BccI	CCATC	3 cut(s)	233, 1049, 1257
BceAI	ACGGC	2 cut(s)	1637, 1692
BcgI	CGANNNNNNTGC	2 cut(s)	30, 64
BciT130I	CCWGG	2 cut(s)	572, 1573
BclI	TGATCA	3 cut(s)	517, 1390, 1416
BcnI	CCSGG	1 cut(s)	1225
BcoDI	GTCTC	3 cut(s)	569, 1172, 1700
BfaI	CTAG	5 cut(s)	89, 510, 599, 1019, 1100
BfmI	CTRYAG	1 cut(s)	307
BfuAI	ACCTGC	2 cut(s)	603, 1408
BglII	AGATCT	1 cut(s)	1307
BmcAI	AGTACT	1 cut(s)	425
Bme1390I	CCNGG	3 cut(s)	572, 1225, 1573
BmeT110I	CYCGRG	2 cut(s)	939, 1703
BmgBI	CACGTC	1 cut(s)	838
BmgT120I	GGNCC	1 cut(s)	263
BmiI	GGNNCC	2 cut(s)	911, 1604
BmrFI	CCNGG	3 cut(s)	572, 1225, 1573
BmrI	ACTGGG	1 cut(s)	1539
BmsI	GCATC	1 cut(s)	136
BmtI	GCTAGC	1 cut(s)	92
BmuI	ACTGGG	1 cut(s)	1539
BpiI	GAAGAC	2 cut(s)	188, 1212
BpmI	CTGGAG	4 cut(s)	593, 1332, 1656, 1677
Bpu14I	TTCGAA	1 cut(s)	749
BpuEI	CTTGAG	1 cut(s)	19
BpuMI	CCSGG	1 cut(s)	1225
BsaI	GGTCTC	2 cut(s)	569, 1700
BsaJI	CCNNGG	5 cut(s)	173, 777, 938, 1049, 1224
BsaWI	WCCGGW	2 cut(s)	142, 1367
Bsc4I	CCNNNNNNNGG	1 cut(s)	1055
Bse118I	RCCGGY	1 cut(s)	1367
Bse1I	ACTGG	1 cut(s)	1545
Bse3DI	GCAATG	2 cut(s)	92, 947
BseAI	TCCGGA	1 cut(s)	142
BseBI	CCWGG	2 cut(s)	572, 1573
BseDI	CCNNGG	5 cut(s)	173, 777, 938, 1049, 1224
BseGI	GGATG	5 cut(s)	151, 362, 634, 1060, 1507
BseLI	CCNNNNNNNGG	1 cut(s)	1055
BseMI	GCAATG	2 cut(s)	92, 947
BseMII	CTCAG	2 cut(s)	762, 819
BseNI	ACTGG	1 cut(s)	1545
BseXI	GCAGC	6 cut(s)	78, 571, 928, 1511, 1546, 1611
BseYI	CCCAGC	1 cut(s)	206
BsgI	GTGCAG	1 cut(s)	1386
Bsh1236I	CGCG	4 cut(s)	1556, 1564, 1578, 1650
BshFI	GGCC	4 cut(s)	202, 265, 776, 1155
BshNI	GGYRCC	2 cut(s)	909, 1602
BshTI	ACCGGT	1 cut(s)	1367
BsiHKAI	GWGCWC	4 cut(s)	35, 321, 1642, 1663
BsiHKCI	CYCGRG	2 cut(s)	939, 1703
BsiSI	CCGG	6 cut(s)	143, 449, 801, 1224, 1368, 1612
BslFI	GGGAC	3 cut(s)	1280, 1526, 1680
BslI	CCNNNNNNNGG	1 cut(s)	1055
BsmAI	GTCTC	3 cut(s)	569, 1172, 1700
BsmFI	GGGAC	3 cut(s)	1280, 1526, 1680
BsmI	GAATGC	2 cut(s)	149, 1427
BsnI	GGCC	4 cut(s)	202, 265, 776, 1155
Bso31I	GGTCTC	2 cut(s)	569, 1700
BsoBI	CYCGRG	2 cut(s)	939, 1703
Bsp119I	TTCGAA	1 cut(s)	749
Bsp1286I	GDGCHC	4 cut(s)	35, 321, 1642, 1663
Bsp13I	TCCGGA	1 cut(s)	142
Bsp1407I	TGTACA	1 cut(s)	997
Bsp143I	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
Bsp19I	CCATGG	1 cut(s)	173
BspACI	CCGC	8 cut(s)	914, 1073, 1110, 1527, 1556, 1609, 1685, 1688
BspANI	GGCC	4 cut(s)	202, 265, 776, 1155
BspCNI	CTCAG	2 cut(s)	763, 818
BspEI	TCCGGA	1 cut(s)	142
BspFNI	CGCG	4 cut(s)	1556, 1564, 1578, 1650
BspHI	TCATGA	1 cut(s)	15
BspLI	GGNNCC	2 cut(s)	911, 1604
BspMI	ACCTGC	2 cut(s)	603, 1408
BspOI	GCTAGC	1 cut(s)	92
BspPI	GGATC	3 cut(s)	479, 876, 1100
BspT104I	TTCGAA	1 cut(s)	749
BspT107I	GGYRCC	2 cut(s)	909, 1602
BspTNI	GGTCTC	2 cut(s)	569, 1700
BsrDI	GCAATG	2 cut(s)	92, 947
BsrFI	RCCGGY	1 cut(s)	1367
BsrGI	TGTACA	1 cut(s)	997
BsrI	ACTGG	1 cut(s)	1545
BssAI	RCCGGY	1 cut(s)	1367
BssECI	CCNNGG	5 cut(s)	173, 777, 938, 1049, 1224
BssMI	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
BssNAI	GTATAC	1 cut(s)	418
BssSI	CACGAG	1 cut(s)	1680
BssT1I	CCWWGG	3 cut(s)	173, 777, 1049
Bst1107I	GTATAC	1 cut(s)	418
Bst2BI	CACGAG	1 cut(s)	1680
Bst2UI	CCWGG	2 cut(s)	572, 1573
Bst4CI	ACNGT	2 cut(s)	568, 1595
BstAPI	GCANNNNNTGC	1 cut(s)	595
BstAUI	TGTACA	1 cut(s)	997
BstBI	TTCGAA	1 cut(s)	749
BstC8I	GCNNGC	9 cut(s)	90, 107, 123, 373, 659, 702, 926, 930, 1249
BstDEI	CTNAG	2 cut(s)	771, 805
BstDSI	CCRYGG	1 cut(s)	173
BstF5I	GGATG	5 cut(s)	151, 362, 634, 1060, 1507
BstFNI	CGCG	4 cut(s)	1556, 1564, 1578, 1650
BstHHI	GCGC	5 cut(s)	88, 706, 1564, 1578, 1650
BstKTI	GATC	9 cut(s)	339, 474, 520, 871, 1108, 1310, 1393, 1419, 1619
BstMAI	GTCTC	3 cut(s)	569, 1172, 1700
BstMBI	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
BstMWI	GCNNNNNNNGC	8 cut(s)	94, 595, 925, 1081, 1109, 1524, 1685, 1694
BstNI	CCWGG	2 cut(s)	572, 1573
BstNSI	RCATGY	4 cut(s)	109, 704, 1534, 1631
BstSCI	CCNGG	3 cut(s)	570, 1223, 1571
BstSFI	CTRYAG	1 cut(s)	307
BstUI	CGCG	4 cut(s)	1556, 1564, 1578, 1650
BstV1I	GCAGC	6 cut(s)	78, 571, 928, 1511, 1546, 1611
BstV2I	GAAGAC	2 cut(s)	188, 1212
BstX2I	RGATCY	3 cut(s)	471, 868, 1307
BstYI	RGATCY	3 cut(s)	471, 868, 1307
BstZ17I	GTATAC	1 cut(s)	418
BsuRI	GGCC	4 cut(s)	202, 265, 776, 1155
BtgI	CCRYGG	1 cut(s)	173
BtrI	CACGTC	1 cut(s)	838
BtsCI	GGATG	5 cut(s)	151, 362, 634, 1060, 1507
BtsI	GCAGTG	1 cut(s)	713
BtsIMutI	CAGTG	3 cut(s)	713, 1386, 1552
BveI	ACCTGC	2 cut(s)	603, 1408
Cac8I	GCNNGC	9 cut(s)	90, 107, 123, 373, 659, 702, 926, 930, 1249
CaiI	CAGNNNCTG	1 cut(s)	995
CciI	TCATGA	1 cut(s)	15
CfoI	GCGC	5 cut(s)	88, 706, 1564, 1578, 1650
Cfr10I	RCCGGY	1 cut(s)	1367
Cfr13I	GGNCC	1 cut(s)	263
Csp6I	GTAC	3 cut(s)	424, 910, 998
CspAI	ACCGGT	1 cut(s)	1367
CviAII	CATG	9 cut(s)	16, 42, 106, 174, 701, 1139, 1531, 1628, 1672
CviQI	GTAC	3 cut(s)	424, 910, 998
DdeI	CTNAG	2 cut(s)	771, 805
DpnI	GATC	9 cut(s)	338, 473, 519, 870, 1107, 1309, 1392, 1418, 1618
DpnII	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
Ecl136II	GAGCTC	2 cut(s)	33, 319
Eco130I	CCWWGG	3 cut(s)	173, 777, 1049
Eco147I	AGGCCT	3 cut(s)	202, 776, 1155
Eco24I	GRGCYC	2 cut(s)	35, 321
Eco31I	GGTCTC	2 cut(s)	569, 1700
Eco53kI	GAGCTC	2 cut(s)	33, 319
Eco57I	CTGAAG	2 cut(s)	884, 1374
Eco88I	CYCGRG	2 cut(s)	939, 1703
EcoICRI	GAGCTC	2 cut(s)	33, 319
EcoRI	GAATTC	3 cut(s)	58, 745, 1508
EcoRII	CCWGG	2 cut(s)	570, 1571
EcoT14I	CCWWGG	3 cut(s)	173, 777, 1049
EcoT22I	ATGCAT	1 cut(s)	151
EcoT38I	GRGCYC	2 cut(s)	35, 321
ErhI	CCWWGG	3 cut(s)	173, 777, 1049
FaeI	CATG	9 cut(s)	19, 45, 109, 177, 704, 1142, 1534, 1631, 1675
FaqI	GGGAC	3 cut(s)	1280, 1526, 1680
FatI	CATG	9 cut(s)	15, 41, 105, 173, 700, 1138, 1530, 1627, 1671
FauI	CCCGC	2 cut(s)	921, 1117
FbaI	TGATCA	3 cut(s)	517, 1390, 1416
FblI	GTMKAC	1 cut(s)	417
FokI	GGATG	5 cut(s)	158, 369, 621, 1067, 1514
FriOI	GRGCYC	2 cut(s)	35, 321
FspBI	CTAG	5 cut(s)	89, 510, 599, 1019, 1100
FspI	TGCGCA	1 cut(s)	705
GlaI	GCGC	5 cut(s)	87, 705, 1563, 1577, 1649
GsaI	CCCAGC	1 cut(s)	210
GsuI	CTGGAG	4 cut(s)	593, 1332, 1656, 1677
HaeIII	GGCC	4 cut(s)	202, 265, 776, 1155
HapII	CCGG	6 cut(s)	143, 449, 801, 1224, 1368, 1612
HhaI	GCGC	5 cut(s)	88, 706, 1564, 1578, 1650
Hin1II	CATG	9 cut(s)	19, 45, 109, 177, 704, 1142, 1534, 1631, 1675
Hin6I	GCGC	5 cut(s)	86, 704, 1562, 1576, 1648
HinP1I	GCGC	5 cut(s)	86, 704, 1562, 1576, 1648
HinfI	GANTC	3 cut(s)	478, 992, 1488
HpaII	CCGG	6 cut(s)	143, 449, 801, 1224, 1368, 1612
HphI	GGTGA	3 cut(s)	346, 793, 884
Hpy166II	GTNNAC	2 cut(s)	418, 1281
Hpy188I	TCNGA	9 cut(s)	155, 249, 483, 495, 808, 835, 991, 1354, 1411
Hpy188III	TCNNGA	7 cut(s)	16, 36, 143, 475, 515, 664, 1311
Hpy8I	GTNNAC	2 cut(s)	418, 1281
Hpy99I	CGWCG	3 cut(s)	263, 1448, 1499
HpyAV	CCTTC	7 cut(s)	21, 648, 661, 859, 997, 1123, 1166
HpyCH4III	ACNGT	2 cut(s)	568, 1595
HpyCH4IV	ACGT	2 cut(s)	837, 1239
HpyF10VI	GCNNNNNNNGC	8 cut(s)	94, 595, 925, 1081, 1109, 1524, 1685, 1694
HpyF3I	CTNAG	2 cut(s)	771, 805
HpySE526I	ACGT	2 cut(s)	837, 1239
Hsp92II	CATG	9 cut(s)	19, 45, 109, 177, 704, 1142, 1534, 1631, 1675
HspAI	GCGC	5 cut(s)	86, 704, 1562, 1576, 1648
Kpn2I	TCCGGA	1 cut(s)	142
KpnI	GGTACC	1 cut(s)	913
Ksp22I	TGATCA	3 cut(s)	517, 1390, 1416
Kzo9I	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
LmnI	GCTCC	6 cut(s)	30, 349, 686, 1411, 1637, 1658
Lsp1109I	GCAGC	6 cut(s)	78, 571, 928, 1511, 1546, 1611
LweI	GCATC	1 cut(s)	136
MaeI	CTAG	5 cut(s)	89, 510, 599, 1019, 1100
MaeII	ACGT	2 cut(s)	837, 1239
MaeIII	GTNAC	3 cut(s)	1547, 1579, 1589
MalI	GATC	9 cut(s)	338, 473, 519, 870, 1107, 1309, 1392, 1418, 1618
MboI	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
MboII	GAAGA	5 cut(s)	193, 290, 1019, 1212, 1483
MflI	RGATCY	3 cut(s)	471, 868, 1307
MhlI	GDGCHC	4 cut(s)	35, 321, 1642, 1663
MmeI	TCCRAC	1 cut(s)	813
Mph1103I	ATGCAT	1 cut(s)	151
MroI	TCCGGA	1 cut(s)	142
MseI	TTAA	3 cut(s)	438, 531, 795
MspA1I	CMGCKG	1 cut(s)	1075
MspI	CCGG	6 cut(s)	143, 449, 801, 1224, 1368, 1612
MspR9I	CCNGG	3 cut(s)	572, 1225, 1573
Mva1269I	GAATGC	2 cut(s)	149, 1427
MvaI	CCWGG	2 cut(s)	572, 1573
MvnI	CGCG	4 cut(s)	1556, 1564, 1578, 1650
MwoI	GCNNNNNNNGC	8 cut(s)	94, 595, 925, 1081, 1109, 1524, 1685, 1694
NciI	CCSGG	1 cut(s)	1225
NcoI	CCATGG	1 cut(s)	173
NdeII	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
NheI	GCTAGC	1 cut(s)	88
NlaIII	CATG	9 cut(s)	19, 45, 109, 177, 704, 1142, 1534, 1631, 1675
NlaIV	GGNNCC	2 cut(s)	911, 1604
NmeAIII	GCCGAG	1 cut(s)	945
NmuCI	GTSAC	3 cut(s)	1547, 1579, 1589
NsbI	TGCGCA	1 cut(s)	705
NsiI	ATGCAT	1 cut(s)	151
NspI	RCATGY	4 cut(s)	109, 704, 1534, 1631
NspV	TTCGAA	1 cut(s)	749
PaeI	GCATGC	2 cut(s)	109, 704
PagI	TCATGA	1 cut(s)	15
PceI	AGGCCT	3 cut(s)	202, 776, 1155
PctI	GAATGC	2 cut(s)	149, 1427
PfeI	GAWTC	3 cut(s)	478, 992, 1488
PflMI	CCANNNNNTGG	1 cut(s)	1055
PfoI	TCCNGGA	1 cut(s)	570
PinAI	ACCGGT	1 cut(s)	1367
PshBI	ATTAAT	1 cut(s)	531
Psp124BI	GAGCTC	2 cut(s)	35, 321
Psp6I	CCWGG	2 cut(s)	570, 1571
PspFI	CCCAGC	1 cut(s)	206
PspGI	CCWGG	2 cut(s)	570, 1571
PspN4I	GGNNCC	2 cut(s)	911, 1604
PspPI	GGNCC	1 cut(s)	263
PstNI	CAGNNNCTG	1 cut(s)	995
PsuI	RGATCY	3 cut(s)	471, 868, 1307
RsaI	GTAC	3 cut(s)	425, 911, 999
RsaNI	GTAC	3 cut(s)	424, 910, 998
SacI	GAGCTC	2 cut(s)	35, 321
SaqAI	TTAA	3 cut(s)	438, 531, 795
Sau3AI	GATC	9 cut(s)	336, 471, 517, 868, 1105, 1307, 1390, 1416, 1616
Sau96I	GGNCC	1 cut(s)	263
ScaI	AGTACT	1 cut(s)	425
ScrFI	CCNGG	3 cut(s)	572, 1225, 1573
SduI	GDGCHC	4 cut(s)	35, 321, 1642, 1663
SfaNI	GCATC	1 cut(s)	136
SfcI	CTRYAG	1 cut(s)	307
SfuI	TTCGAA	1 cut(s)	749
SmlI	CTYRAG	1 cut(s)	34
SmoI	CTYRAG	1 cut(s)	34
SphI	GCATGC	2 cut(s)	109, 704
SseBI	AGGCCT	3 cut(s)	202, 776, 1155
SsiI	CCGC	8 cut(s)	914, 1073, 1110, 1527, 1556, 1609, 1685, 1688
SspI	AATATT	2 cut(s)	12, 970
SspMI	CTAG	5 cut(s)	89, 510, 599, 1019, 1100
SstI	GAGCTC	2 cut(s)	35, 321
StuI	AGGCCT	3 cut(s)	202, 776, 1155
StyD4I	CCNGG	3 cut(s)	570, 1223, 1571
StyI	CCWWGG	3 cut(s)	173, 777, 1049
TaaI	ACNGT	2 cut(s)	568, 1595
TaiI	ACGT	2 cut(s)	840, 1242
TaqI	TCGA	6 cut(s)	225, 412, 749, 1185, 1443, 1497
TaqII	GACCGA	1 cut(s)	272
TatI	WGTACW	2 cut(s)	423, 997
TauI	GCSGC	4 cut(s)	1530, 1559, 1688, 1691
TfiI	GAWTC	3 cut(s)	478, 992, 1488
Tru1I	TTAA	3 cut(s)	438, 531, 795
Tru9I	TTAA	3 cut(s)	438, 531, 795
TscAI	CASTG	3 cut(s)	713, 1393, 1552
TseFI	GTSAC	3 cut(s)	1547, 1579, 1589
TseI	GCWGC	6 cut(s)	66, 559, 916, 1524, 1559, 1624
Tsp45I	GTSAC	3 cut(s)	1547, 1579, 1589
TspDTI	ATGAA	8 cut(s)	4, 32, 259, 377, 408, 825, 1155, 1484
TspRI	CASTG	3 cut(s)	713, 1393, 1552
Van91I	CCANNNNNTGG	1 cut(s)	1055
VspI	ATTAAT	1 cut(s)	531
XapI	RAATTY	7 cut(s)	58, 233, 281, 745, 957, 1480, 1508
XceI	RCATGY	4 cut(s)	109, 704, 1534, 1631
XmiI	GTMKAC	1 cut(s)	417
XspI	CTAG	5 cut(s)	89, 510, 599, 1019, 1100
ZrmI	AGTACT	1 cut(s)	425
Zsp2I	ATGCAT	1 cut(s)	151

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.