pycom06g12600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008157
Physical Location & Seq
Forward (+)
17723995 .. 17724603
609 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12600.1

Sequence Viewer

Length: 609 bp
ATGGAAGCAGAAAGGAGGCATGAAGCTGTGCAAGGCCTTCGCAATCTTACTATTGTGGAGACAACCGAACCAATCAATGTAAGCTATAACTTACCATTGTCTTCCGGGGAGGTTTTGAACGTGAGGGCAAGCCGAAAGATACGATGGGACAAAGAAAAGTCCACATTTTTCACACAGAAAATGGACAGATCTGGAGGGCAGAAAAGTGTCATCGAGTTTGCCACATATTTCTCTGAAGTAATATCTGCCGGTGTCCTGTGGGAGAACACTGATCATATACCTGCACTTTCGGAGCTGATCAAGTTGGCATTCGTGCTGGATTTCGACGAGGAAGCAGTTGAGTTCTATATGAAGTCCAAGAATTTTCGAATCTTCGTCGAGGATGAGGAGTTCCTCGGCGAAAGTGGCGGCACGTTCTCTGTCGGCATGTTTGCTGTCGGCAGCGACCATGCAGCCGCGCGTGTTTCCAGGCACGTGACAGCGCGTGACGGCACGTGGCACCACCGCAGCGGATCACGGATGCATGTGGCTGGAGCACGGAGGCGGGTGGCTGGAGCACGGTGGCGCGTGGTGACTCGTGCGGCGGTAAGTGCAGGACCCGAGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

22.65

Weight (kDa)

7.08

Isoelectric Point (pI)

56.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 289
AccB1I GGYRCC 1 cut(s) 498
AccII CGCG 4 cut(s) 458, 460, 484, 567
AciI CCGC 7 cut(s) 408, 456, 505, 510, 544, 581, 584
AclWI GGATC 1 cut(s) 520
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 255
AcvI CACGTG 2 cut(s) 475, 495
AgsI TTSAA 1 cut(s) 118
AjnI CCWGG 1 cut(s) 467
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 3 cut(s) 26, 84, 295
AluI AGCT 3 cut(s) 26, 84, 295
Alw21I GWGCWC 2 cut(s) 538, 559
Alw26I GTCTC 2 cut(s) 53, 596
AlwI GGATC 1 cut(s) 520
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 3 cut(s) 441, 452, 507
ApoI RAATTY 1 cut(s) 361
AspLEI GCGC 3 cut(s) 460, 484, 567
AspS9I GGNCC 1 cut(s) 596
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 583
AsuII TTCGAA 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 1 cut(s) 596
BanI GGYRCC 1 cut(s) 498
BauI CACGAG 1 cut(s) 576
BbrPI CACGTG 2 cut(s) 475, 495
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 538, 559
BbvI GCAGC 3 cut(s) 453, 464, 519
BccI CCATC 1 cut(s) 138
BceAI ACGGC 1 cut(s) 505
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 469
BclI TGATCA 2 cut(s) 271, 297
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 53, 596
BfuAI ACCTGC 1 cut(s) 289
BglII AGATCT 1 cut(s) 188
BisI GCNGC 6 cut(s) 409, 442, 453, 456, 508, 582
BlsI GCNGC 6 cut(s) 410, 443, 454, 457, 509, 583
Bme1390I CCNGG 2 cut(s) 106, 469
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 2 cut(s) 500, 598
BmrFI CCNGG 2 cut(s) 106, 469
BmsI GCATC 1 cut(s) 510
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 3 cut(s) 213, 552, 573
Bpu14I TTCGAA 1 cut(s) 367
BpuMI CCSGG 1 cut(s) 106
BsaAI YACGTR 2 cut(s) 475, 495
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 105, 394
Bse118I RCCGGY 1 cut(s) 248
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 2 cut(s) 105, 394
BseGI GGATG 2 cut(s) 388, 525
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 3 cut(s) 453, 464, 519
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 4 cut(s) 458, 460, 484, 567
BshFI GGCC 1 cut(s) 36
BshNI GGYRCC 1 cut(s) 498
BsiHKAI GWGCWC 2 cut(s) 538, 559
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 2 cut(s) 105, 249
BslFI GGGAC 1 cut(s) 161
BsmAI GTCTC 2 cut(s) 53, 596
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 36
Bso31I GGTCTC 1 cut(s) 596
BsoBI CYCGRG 1 cut(s) 599
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 538, 559
Bsp143I GATC 4 cut(s) 188, 271, 297, 512
BspACI CCGC 7 cut(s) 408, 456, 505, 510, 544, 581, 584
BspANI GGCC 1 cut(s) 36
BspFNI CGCG 4 cut(s) 458, 460, 484, 567
BspLI GGNNCC 2 cut(s) 500, 598
BspMI ACCTGC 1 cut(s) 289
BspPI GGATC 1 cut(s) 520
BspT104I TTCGAA 1 cut(s) 367
BspT107I GGYRCC 1 cut(s) 498
BspTNI GGTCTC 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 105, 394
BssMI GATC 4 cut(s) 188, 271, 297, 512
BssSI CACGAG 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 576
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 561
BstBAI YACGTR 2 cut(s) 475, 495
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 130
BstF5I GGATG 2 cut(s) 388, 525
BstFNI CGCG 4 cut(s) 458, 460, 484, 567
BstHHI GCGC 3 cut(s) 460, 484, 567
BstKTI GATC 4 cut(s) 191, 274, 300, 515
BstMAI GTCTC 2 cut(s) 53, 596
BstMBI GATC 4 cut(s) 188, 271, 297, 512
BstMWI GCNNNNNNNGC 2 cut(s) 405, 590
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 2 cut(s) 430, 527
BstSCI CCNGG 2 cut(s) 104, 467
BstUI CGCG 4 cut(s) 458, 460, 484, 567
BstV1I GCAGC 3 cut(s) 453, 464, 519
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 1 cut(s) 36
BtsCI GGATG 2 cut(s) 388, 525
BtsIMutI CAGTG 1 cut(s) 267
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 130
CfoI GCGC 3 cut(s) 460, 484, 567
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 596
CviAII CATG 4 cut(s) 20, 427, 449, 524
CviJI RGCY 8 cut(s) 26, 36, 84, 132, 295, 455, 530, 551
CviKI_1 RGCY 8 cut(s) 26, 36, 84, 132, 295, 455, 530, 551
DpnI GATC 4 cut(s) 190, 273, 299, 514
DpnII GATC 4 cut(s) 188, 271, 297, 512
Eco147I AGGCCT 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 596
Eco47I GGWCC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 255
Eco72I CACGTG 2 cut(s) 475, 495
Eco88I CYCGRG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 467
EcoT22I ATGCAT 1 cut(s) 525
FaeI CATG 4 cut(s) 23, 430, 452, 527
FaqI GGGAC 1 cut(s) 161
FatI CATG 4 cut(s) 19, 426, 448, 523
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 2 cut(s) 271, 297
Fnu4HI GCNGC 6 cut(s) 409, 442, 453, 456, 508, 582
FokI GGATG 2 cut(s) 395, 532
Fsp4HI GCNGC 6 cut(s) 409, 442, 453, 456, 508, 582
GlaI GCGC 3 cut(s) 459, 483, 566
GluI GCNGC 6 cut(s) 409, 442, 453, 456, 508, 582
GsuI CTGGAG 3 cut(s) 213, 552, 573
HaeIII GGCC 1 cut(s) 36
HapII CCGG 2 cut(s) 105, 249
HhaI GCGC 3 cut(s) 460, 484, 567
Hin1II CATG 4 cut(s) 23, 430, 452, 527
Hin6I GCGC 3 cut(s) 458, 482, 565
HinP1I GCGC 3 cut(s) 458, 482, 565
HinfI GANTC 2 cut(s) 369, 574
HpaII CCGG 2 cut(s) 105, 249
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 2 cut(s) 235, 292
Hpy188III TCNNGA 1 cut(s) 192
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 2 cut(s) 329, 380
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4IV ACGT 4 cut(s) 120, 413, 474, 494
HpyCH4V TGCA 5 cut(s) 31, 284, 452, 523, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 405, 590
HpySE526I ACGT 4 cut(s) 120, 413, 474, 494
Hsp92II CATG 4 cut(s) 23, 430, 452, 527
HspAI GCGC 3 cut(s) 458, 482, 565
Ksp22I TGATCA 2 cut(s) 271, 297
Kzo9I GATC 4 cut(s) 188, 271, 297, 512
LmnI GCTCC 3 cut(s) 292, 533, 554
Lsp1109I GCAGC 3 cut(s) 453, 464, 519
LweI GCATC 1 cut(s) 510
MaeII ACGT 4 cut(s) 120, 413, 474, 494
MaeIII GTNAC 3 cut(s) 475, 485, 571
MalI GATC 4 cut(s) 190, 273, 299, 514
MboI GATC 4 cut(s) 188, 271, 297, 512
MboII GAAGA 2 cut(s) 93, 364
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 2 cut(s) 538, 559
MluCI AATT 1 cut(s) 361
MlyI GAGTC 1 cut(s) 568
MnlI CCTC 9 cut(s) 9, 103, 117, 188, 322, 373, 379, 404, 534
Mph1103I ATGCAT 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 2 cut(s) 105, 249
MspR9I CCNGG 2 cut(s) 106, 469
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 1 cut(s) 469
MvnI CGCG 4 cut(s) 458, 460, 484, 567
MwoI GCNNNNNNNGC 2 cut(s) 405, 590
NciI CCSGG 1 cut(s) 106
NdeII GATC 4 cut(s) 188, 271, 297, 512
NlaIII CATG 4 cut(s) 23, 430, 452, 527
NlaIV GGNNCC 2 cut(s) 500, 598
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 3 cut(s) 475, 485, 571
NsiI ATGCAT 1 cut(s) 525
NspI RCATGY 2 cut(s) 430, 527
NspV TTCGAA 1 cut(s) 367
PceI AGGCCT 1 cut(s) 36
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 369
PkrI GCNGC 6 cut(s) 410, 443, 454, 457, 509, 583
PleI GAGTC 1 cut(s) 568
PmaCI CACGTG 2 cut(s) 475, 495
PmlI CACGTG 2 cut(s) 475, 495
PpsI GAGTC 1 cut(s) 568
Ppu21I YACGTR 2 cut(s) 475, 495
PpuMI RGGWCCY 1 cut(s) 596
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 467
PspCI CACGTG 2 cut(s) 475, 495
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 500, 598
PspPI GGNCC 1 cut(s) 596
PspPPI RGGWCCY 1 cut(s) 596
PsuI RGATCY 1 cut(s) 188
SatI GCNGC 6 cut(s) 409, 442, 453, 456, 508, 582
Sau3AI GATC 4 cut(s) 188, 271, 297, 512
Sau96I GGNCC 1 cut(s) 596
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 2 cut(s) 106, 469
SduI GDGCHC 2 cut(s) 538, 559
SfaNI GCATC 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 367
SinI GGWCC 1 cut(s) 596
Sse9I AATT 1 cut(s) 361
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 7 cut(s) 408, 456, 505, 510, 544, 581, 584
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 104, 467
TaaI ACNGT 1 cut(s) 561
TaiI ACGT 4 cut(s) 123, 416, 477, 497
TaqI TCGA 4 cut(s) 213, 324, 367, 378
TasI AATT 1 cut(s) 361
TauI GCSGC 3 cut(s) 411, 458, 584
TfiI GAWTC 1 cut(s) 369
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 3 cut(s) 475, 485, 571
TseI GCWGC 3 cut(s) 441, 452, 507
Tsp45I GTSAC 3 cut(s) 475, 485, 571
TspDTI ATGAA 2 cut(s) 36, 365
TspGWI ACGGA 2 cut(s) 532, 553
TspRI CASTG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 596
XapI RAATTY 1 cut(s) 361
XceI RCATGY 2 cut(s) 430, 527
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.