MD06G1148600.v1.1

amino acid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005950
Physical Location & Seq
Reverse (-)
29134455 .. 29137704
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1148600.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGGAGGAAGTGGAGAAATTAGAGAGGAGGAGCAGCAGAAGGAGGTGCAAGATATCAACGACTGGCTGCCGATCACGAAATCACGGAACGCGACATGGTGGTATTCGGCATTCCACAATGTCACTGCCATGGTTGGTGCTGGTGTTCTTGGCCTCCCCTATGCCATGTCTCACCTTGGATGGGGCCCTGGAGTTGCAGTGCTGGTTCTCTCATGGATAATTACACTCTACACCTTATGGCAGATGGTAGAAATGCATGAAATTGTACCAGGCAAGAGGTTTGATAGGTACCATGAGTTAGGGCAACATGCCTTTGGGGAAAAGCTTGGGCTTTACATTGTGGTGCCCCAGCAACTGATGGTGGAACTTGGAACTTGCATAGTTTATATGATTACAGGTGGCAATTCCTTGAAGAAGATTCACAGTACTGTGTGTCCTGACTGTAAGCCTATCAAAACCAGCTACTTCATCATGATCTTTGCATCTGTTCACTTTGTTCTCTCCCACCTCCCTAGCCTCAATTCCATTGCTAGTGTTTCTCTTGCTGCTGCTGTCATGTCATTGAGCTACTCTACCATTGCTTGGGGAGCTTCTCTGCACAAAGGGGTGGAGCCACAAGTGCAGTATGGTCCCAGGGCTTCAACCCCTGCAGGGAATACGTTCAACTTCTTTAGTGCTTTGGGAGACGTAGCTTTTGCTTTTGCTGGCCACAATGTGGTTTTGGAGATTCAAGCAACTATTCCTTCCACGCCTGAAAAGCCGTCCAAAAAACCAATGTGGAAGGGGGTCGTTCTTGCTTACATTGTGGTCGCAATGTGCTATTTCCCCGTCGCTCTCATCGGATACTGGGTGTTTGGAAACCACGTCGAGGATAATATCCTCATATCGTTGGAGAAGCCTGCCTGGCTTATTGTTCTTGCGAATTGCTTTGTTGTTGTTCATGTCATTGGAAGTTATCAGGTCTACGCAATGCCGGTGTTTGACATGGCGGAATTATTTCTGTTGAAGAAGATGAAATTCAAGCCATCTTTGGTCCTTCGCTTCGTAACACGAAATGTGATTGTTGCATTCACCTTGTTCGTTGGAATTACATTCCCGTTCTTTGGTGGGCTTCTTGGTTTCTTCGGAGGATTTGCATTTGCTCCTACAACATACTATCTCCCTTGCATCATGTGGCTTATCATCTACAAACCAAAGAAGTTTGGTCTTTCTTGGTTTGCAAATTGGTTCTGCATCATAGTTGGAGTGTCACTGATGCTTTTAGCTCCGATCGGGGCATTAAGGAACCTCATACTTCAAGCGAAGAACTTCAAATTCTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

442

Amino Acids

49.12

Weight (kDa)

8.98

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 30 - 426 1.9e-110 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 41 - 169 6.7e-08 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 288
AccB1I GGYRCC 2 cut(s) 288, 343
AccB7I CCANNNNNTGG 2 cut(s) 582, 715
AccI GTMKAC 1 cut(s) 963
AccII CGCG 1 cut(s) 92
AciI CCGC 1 cut(s) 989
AcoI YGGCCR 1 cut(s) 706
AcsI RAATTY 2 cut(s) 1016, 1313
AdeI CACNNNGTG 1 cut(s) 715
AfaI GTAC 3 cut(s) 267, 290, 427
AfiI CCNNNNNNNGG 6 cut(s) 181, 582, 651, 715, 868, 1103
AgsI TTSAA 8 cut(s) 412, 642, 664, 731, 1006, 1021, 1298, 1312
AjiI CACGTC 1 cut(s) 865
AjnI CCWGG 4 cut(s) 187, 268, 632, 902
AluBI AGCT 6 cut(s) 325, 462, 567, 590, 692, 1265
AluI AGCT 6 cut(s) 325, 462, 567, 590, 692, 1265
Alw26I GTCTC 2 cut(s) 174, 678
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 3 cut(s) 151, 184, 706
ApaI GGGCCC 1 cut(s) 188
ApeKI GCWGC 4 cut(s) 34, 67, 545, 548
ApoI RAATTY 2 cut(s) 1016, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp700I GAANNNNTTC 3 cut(s) 659, 996, 1307
Asp718I GGTACC 1 cut(s) 288
AspS9I GGNCC 4 cut(s) 184, 185, 629, 1033
AsuHPI GGTGA 2 cut(s) 164, 1063
AvaII GGWCC 2 cut(s) 629, 1033
BaeGI GKGCMC 2 cut(s) 188, 348
BalI TGGCCA 1 cut(s) 708
BanI GGYRCC 2 cut(s) 288, 343
BanII GRGCYC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 46, 54, 532, 535
BccI CCATC 4 cut(s) 174, 238, 352, 1033
BceAI ACGGC 1 cut(s) 745
BciT130I CCWGG 4 cut(s) 189, 270, 634, 904
BciVI GTATCC 1 cut(s) 836
BcoDI GTCTC 2 cut(s) 174, 678
BfaI CTAG 2 cut(s) 513, 531
BfmI CTRYAG 1 cut(s) 648
BfuI GTATCC 1 cut(s) 836
BglI GCCNNNNNGGC 1 cut(s) 904
BisI GCNGC 4 cut(s) 35, 68, 546, 549
BlsI GCNGC 4 cut(s) 36, 69, 547, 550
BmcAI AGTACT 1 cut(s) 427
Bme1390I CCNGG 4 cut(s) 189, 270, 634, 904
Bme18I GGWCC 2 cut(s) 629, 1033
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 4 cut(s) 184, 185, 629, 1033
BmiI GGNNCC 7 cut(s) 185, 186, 290, 345, 612, 631, 1286
BmrFI CCNGG 4 cut(s) 189, 270, 634, 904
BmrI ACTGGG 1 cut(s) 856
BmsI GCATC 4 cut(s) 491, 1176, 1242, 1245
BmuI ACTGGG 1 cut(s) 856
BpmI CTGGAG 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 129, 175, 187, 632, 633
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 6 cut(s) 181, 582, 651, 715, 868, 1103
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 2 cut(s) 68, 851
Bse3DI GCAATG 4 cut(s) 525, 576, 819, 975
BseBI CCWGG 4 cut(s) 189, 270, 634, 904
BseDI CCNNGG 5 cut(s) 129, 175, 187, 632, 633
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 6 cut(s) 181, 582, 651, 715, 868, 1103
BseMI GCAATG 4 cut(s) 525, 576, 819, 975
BseNI ACTGG 2 cut(s) 68, 851
BseRI GAGGAG 2 cut(s) 41, 44
BseSI GKGCMC 2 cut(s) 188, 348
BseXI GCAGC 4 cut(s) 46, 54, 532, 535
BseYI CCCAGC 1 cut(s) 348
BsgI GTGCAG 2 cut(s) 581, 641
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 1272
BshFI GGCC 3 cut(s) 153, 186, 708
BshNI GGYRCC 2 cut(s) 288, 343
BsiEI CGRYCG 1 cut(s) 1272
BsiSI CCGG 1 cut(s) 974
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 6 cut(s) 181, 582, 651, 715, 868, 1103
BsmAI GTCTC 2 cut(s) 174, 678
BsmBI CGTCTC 1 cut(s) 678
BsmFI GGGAC 1 cut(s) 615
BsmI GAATGC 2 cut(s) 110, 1067
BsnI GGCC 3 cut(s) 153, 186, 708
Bsp120I GGGCCC 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 188, 348
Bsp143I GATC 3 cut(s) 72, 474, 1269
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 1 cut(s) 989
BspANI GGCC 3 cut(s) 153, 186, 708
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 7 cut(s) 185, 186, 290, 345, 612, 631, 1286
BspMAI CTGCAG 1 cut(s) 652
BspT107I GGYRCC 2 cut(s) 288, 343
BsrDI GCAATG 4 cut(s) 525, 576, 819, 975
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 2 cut(s) 68, 851
BssAI RCCGGY 1 cut(s) 973
BssECI CCNNGG 5 cut(s) 129, 175, 187, 632, 633
BssMI GATC 3 cut(s) 72, 474, 1269
BssT1I CCWWGG 2 cut(s) 129, 175
Bst2UI CCWGG 4 cut(s) 189, 270, 634, 904
Bst4CI ACNGT 3 cut(s) 425, 430, 443
BstC8I GCNNGC 2 cut(s) 706, 900
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 185
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 3 cut(s) 75, 477, 1272
BstMAI GTCTC 2 cut(s) 174, 678
BstMBI GATC 3 cut(s) 72, 474, 1269
BstMCI CGRYCG 1 cut(s) 1272
BstMWI GCNNNNNNNGC 4 cut(s) 587, 619, 757, 904
BstNI CCWGG 4 cut(s) 189, 270, 634, 904
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 187, 268, 632, 902
BstSFI CTRYAG 1 cut(s) 648
BstSLI GKGCMC 2 cut(s) 188, 348
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 4 cut(s) 46, 54, 532, 535
BsuI GTATCC 1 cut(s) 836
BsuRI GGCC 3 cut(s) 153, 186, 708
BtgI CCRYGG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 1 cut(s) 185
BtsI GCAGTG 2 cut(s) 123, 204
BtsIMutI CAGTG 3 cut(s) 123, 204, 1250
Cac8I GCNNGC 2 cut(s) 706, 900
CaiI CAGNNNCTG 1 cut(s) 355
CciI TCATGA 1 cut(s) 471
Cfr10I RCCGGY 1 cut(s) 973
Cfr13I GGNCC 4 cut(s) 184, 185, 629, 1033
Csp6I GTAC 3 cut(s) 266, 289, 426
CviQI GTAC 3 cut(s) 266, 289, 426
DpnI GATC 3 cut(s) 74, 476, 1271
DpnII GATC 3 cut(s) 72, 474, 1269
DraIII CACNNNGTG 1 cut(s) 715
EaeI YGGCCR 1 cut(s) 706
EciI GGCGGA 1 cut(s) 1004
Eco130I CCWWGG 2 cut(s) 129, 175
Eco24I GRGCYC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 629, 1033
EcoO109I RGGNCCY 2 cut(s) 184, 185
EcoRII CCWGG 4 cut(s) 187, 268, 632, 902
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 129, 175
EcoT22I ATGCAT 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 2 cut(s) 129, 175
Esp3I CGTCTC 1 cut(s) 678
FaqI GGGAC 1 cut(s) 615
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 4 cut(s) 35, 68, 546, 549
FokI GGATG 1 cut(s) 192
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 4 cut(s) 35, 68, 546, 549
FspBI CTAG 2 cut(s) 513, 531
GluI GCNGC 4 cut(s) 35, 68, 546, 549
GsaI CCCAGC 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 3 cut(s) 153, 186, 708
HapII CCGG 1 cut(s) 974
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 2 cut(s) 418, 727
HpaII CCGG 1 cut(s) 974
HphI GGTGA 2 cut(s) 164, 1063
Hpy166II GTNNAC 2 cut(s) 490, 964
Hpy188I TCNGA 3 cut(s) 842, 1127, 1269
Hpy188III TCNNGA 4 cut(s) 76, 437, 472, 1323
Hpy8I GTNNAC 2 cut(s) 490, 964
Hpy99I CGWCG 2 cut(s) 833, 869
HpyAV CCTTC 4 cut(s) 34, 753, 775, 1046
HpyCH4III ACNGT 3 cut(s) 425, 430, 443
HpyCH4IV ACGT 3 cut(s) 659, 687, 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 587, 619, 757, 904
HpySE526I ACGT 3 cut(s) 659, 687, 864
KpnI GGTACC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 72, 474, 1269
LmnI GCTCC 5 cut(s) 31, 587, 610, 1147, 1270
Lsp1109I GCAGC 4 cut(s) 46, 54, 532, 535
LweI GCATC 4 cut(s) 491, 1176, 1242, 1245
MaeI CTAG 2 cut(s) 513, 531
MaeII ACGT 3 cut(s) 659, 687, 864
MaeIII GTNAC 3 cut(s) 121, 1045, 1248
MalI GATC 3 cut(s) 74, 476, 1271
MboI GATC 3 cut(s) 72, 474, 1269
MboII GAAGA 6 cut(s) 424, 427, 1018, 1021, 1114, 1315
MhlI GDGCHC 2 cut(s) 188, 348
MlsI TGGCCA 1 cut(s) 708
MluNI TGGCCA 1 cut(s) 708
MmeI TCCRAC 3 cut(s) 870, 1063, 1222
Mox20I TGGCCA 1 cut(s) 708
Mph1103I ATGCAT 1 cut(s) 258
MroXI GAANNNNTTC 3 cut(s) 659, 996, 1307
MscI TGGCCA 1 cut(s) 708
MseI TTAA 1 cut(s) 1280
MslI CAYNNNNRTG 2 cut(s) 128, 341
Msp20I TGGCCA 1 cut(s) 708
MspI CCGG 1 cut(s) 974
MspR9I CCNGG 4 cut(s) 189, 270, 634, 904
Mva1269I GAATGC 2 cut(s) 110, 1067
MvaI CCWGG 4 cut(s) 189, 270, 634, 904
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 587, 619, 757, 904
NcoI CCATGG 1 cut(s) 129
NdeII GATC 3 cut(s) 72, 474, 1269
NlaIV GGNNCC 7 cut(s) 185, 186, 290, 345, 612, 631, 1286
NmuCI GTSAC 2 cut(s) 121, 1248
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 1 cut(s) 311
PagI TCATGA 1 cut(s) 471
PasI CCCWGGG 1 cut(s) 633
PctI GAATGC 2 cut(s) 110, 1067
PdmI GAANNNNTTC 3 cut(s) 659, 996, 1307
PfeI GAWTC 2 cut(s) 418, 727
PflMI CCANNNNNTGG 2 cut(s) 582, 715
PkrI GCNGC 4 cut(s) 36, 69, 547, 550
Ple19I CGATCG 1 cut(s) 1272
Psp6I CCWGG 4 cut(s) 187, 268, 632, 902
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 4 cut(s) 187, 268, 632, 902
PspN4I GGNNCC 7 cut(s) 185, 186, 290, 345, 612, 631, 1286
PspOMI GGGCCC 1 cut(s) 184
PspPI GGNCC 4 cut(s) 184, 185, 629, 1033
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 355
PvuI CGATCG 1 cut(s) 1272
RsaI GTAC 3 cut(s) 267, 290, 427
RsaNI GTAC 3 cut(s) 266, 289, 426
RseI CAYNNNNRTG 2 cut(s) 128, 341
SaqAI TTAA 1 cut(s) 1280
SatI GCNGC 4 cut(s) 35, 68, 546, 549
Sau3AI GATC 3 cut(s) 72, 474, 1269
Sau96I GGNCC 4 cut(s) 184, 185, 629, 1033
SbfI CCTGCAGG 1 cut(s) 652
ScaI AGTACT 1 cut(s) 427
ScrFI CCNGG 4 cut(s) 189, 270, 634, 904
SdaI CCTGCAGG 1 cut(s) 652
SduI GDGCHC 2 cut(s) 188, 348
SfaNI GCATC 4 cut(s) 491, 1176, 1242, 1245
SfcI CTRYAG 1 cut(s) 648
SinI GGWCC 2 cut(s) 629, 1033
SmiMI CAYNNNNRTG 2 cut(s) 128, 341
Sse8387I CCTGCAGG 1 cut(s) 652
SsiI CCGC 1 cut(s) 989
SspMI CTAG 2 cut(s) 513, 531
StyD4I CCNGG 4 cut(s) 187, 268, 632, 902
StyI CCWWGG 2 cut(s) 129, 175
TaaI ACNGT 3 cut(s) 425, 430, 443
TaiI ACGT 3 cut(s) 662, 690, 867
TaqI TCGA 1 cut(s) 867
TatI WGTACW 1 cut(s) 425
TfiI GAWTC 2 cut(s) 418, 727
Tru1I TTAA 1 cut(s) 1280
Tru9I TTAA 1 cut(s) 1280
TscAI CASTG 3 cut(s) 130, 204, 1257
TseFI GTSAC 2 cut(s) 121, 1248
TseI GCWGC 4 cut(s) 34, 67, 545, 548
Tsp45I GTSAC 2 cut(s) 121, 1248
TspDTI ATGAA 4 cut(s) 273, 457, 929, 1028
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 3 cut(s) 130, 204, 1257
Van91I CCANNNNNTGG 2 cut(s) 582, 715
VpaK11BI GGWCC 2 cut(s) 629, 1033
XapI RAATTY 2 cut(s) 1016, 1313
XceI RCATGY 1 cut(s) 311
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 3 cut(s) 659, 996, 1307
XspI CTAG 2 cut(s) 513, 531
ZrmI AGTACT 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.