Rw5G035360

amino acid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005950
Physical Location & Seq
Reverse (-)
61139880 .. 61144055
4176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035360.1

Sequence Viewer

Length: 1314 bp
ATGATGCCAAAAAAACGATCAGAGCTGCATTTTAAGGAAAAGGATCTGAATGATTGGCTTCCCATCACAAGTTCTAGGACTGCAACCTGGTGGTACTCAGCTTTCCACAATGTCACCGCCATGGTTGGTGCTGGTGTGCTTGGTCTTCCCTTTGCCATGTCTCAACTTGGATGGGGACCTGGAGTTACTGTGATTGTTCTTTCATGGATTATCACCCTATACACCTTATGGCAGATGGTAGAGATGCATGAGTCGGTGCCGGGGAAGAGGTTTGATAGGTACCATGAGTTGGGTCAACATGCCTTCGGAGAAAAGCTAGGGCTTTGGATTGTGGTACCCCAACAACTAATGGTGCAATCTGGTACGTGCATCGTCTATATGATTACTGGTGGTAAATCACTGCAGAAGATCCATGATACCGTGTCCCCTGGAAAACCAATCAAAACCACCTACTTCATCATGATCTTCGCTGCTGTCCACTTTGTTATCTCTCACCTCCCTAGTTTCAATTCCATTGCTGGTATTTCTCTTACTGCAGCTGTCATGTCCTTGAGCTACTCTACAGTTGCATGGGGAGCTTCACTTCACAAAGGGGTTCAACCAGAAGTGCAGTATGGCCCCAGGGCTTCGACCAACACAGGAAATGTGTTCAACTTCTTTAGTGCTTTGGGAGATGTAGCTTTTTCTTTTGCTGGTCACAATGTGGTTTTGGAGATTCAAGCAACACTTCCTTCTACACCTGACAAGCCTTCCAAAAAACCAATGTGGAAGGGTGTGGTGCTTGCATATGTTGTGGTGGCTTTGTGCTATTTCCCAGTTGCTCTTATCGGTTATTGGGTGTTCGGAAACAGTGTTCAGGATAATATCCTCATATCATTGGAGAAACCTAAGTGGCTTATTGTTTCTGCTAATTCCTTTGTTGTTATTCATGTCATTGGGAGCTATCAGGTCTATGCAATGCCGGTGTTTGACACAATTGAAGCAGCTCTAGTGAAACAGATGAATTTCAAACCATCTTATTCTCTCCGCTTTATATCACGAAATCTATTTGTTGCAATGACCTTGTTCATCGGAGTCACGTTCCCTTTCTTTGGCGGGCTTCTCAGTTTCTTTGGAGGATTTGCTTTGGCTCCCACAACATATTTTCTTCCTTGCATCATGTGGCTCGTCATCTACAAACCTAAGAAGTTCAGCTTATCCTGGTTTGCAAATTGGTTCTGCATTATAGCTGGAGTGTTATTGCTGATTTTTGCTCCTATTGGTGCATTGAGGAATATCATACTTCAAGCCAAGGATTTCAAATTCTACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

437

Amino Acids

48.73

Weight (kDa)

9.46

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 26 - 422 2.6e-106 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 279, 334
AccB1I GGYRCC 3 cut(s) 256, 279, 334
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 3 cut(s) 117, 1027, 1095
AclWI GGATC 2 cut(s) 51, 403
AcsI RAATTY 2 cut(s) 1003, 1301
AdeI CACNNNGTG 1 cut(s) 703
AfaI GTAC 4 cut(s) 95, 281, 336, 364
AfiI CCNNNNNNNGG 2 cut(s) 289, 1091
AgsI TTSAA 8 cut(s) 508, 599, 652, 719, 980, 1009, 1286, 1300
AjnI CCWGG 5 cut(s) 86, 178, 427, 620, 1199
AloI GAACNNNNNNTCC 2 cut(s) 837, 869
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 2 cut(s) 51, 403
AoxI GGCC 1 cut(s) 616
ApeKI GCWGC 4 cut(s) 25, 470, 536, 983
ApoI RAATTY 2 cut(s) 1003, 1301
Asp718I GGTACC 2 cut(s) 279, 334
AspS9I GGNCC 2 cut(s) 176, 617
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 3 cut(s) 106, 205, 485
AvaII GGWCC 1 cut(s) 176
BanI GGYRCC 3 cut(s) 256, 279, 334
BbsI GAAGAC 1 cut(s) 137
BbvI GCAGC 4 cut(s) 12, 457, 548, 995
BccI CCATC 4 cut(s) 71, 165, 229, 1021
BciT130I CCWGG 5 cut(s) 88, 180, 429, 622, 1201
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 4 cut(s) 75, 317, 501, 989
BfmI CTRYAG 3 cut(s) 401, 534, 561
BisI GCNGC 4 cut(s) 26, 471, 537, 984
BlsI GCNGC 4 cut(s) 27, 472, 538, 985
Bme1390I CCNGG 6 cut(s) 88, 180, 261, 429, 622, 1201
Bme18I GGWCC 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 176, 617
BmiI GGNNCC 6 cut(s) 177, 258, 281, 336, 619, 1131
BmrFI CCNGG 6 cut(s) 88, 180, 261, 429, 622, 1201
BmrI ACTGGG 1 cut(s) 809
BmsI GCATC 3 cut(s) 234, 378, 1164
BmuI ACTGGG 1 cut(s) 809
BpiI GAAGAC 1 cut(s) 137
BpmI CTGGAG 2 cut(s) 201, 1251
BpuEI CTTGAG 1 cut(s) 571
BpuMI CCSGG 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 366
BsaJI CCNNGG 6 cut(s) 120, 260, 427, 620, 621, 1290
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 1091
Bse118I RCCGGY 1 cut(s) 961
Bse1I ACTGG 2 cut(s) 391, 815
Bse3DI GCAATG 3 cut(s) 513, 963, 1062
BseBI CCWGG 5 cut(s) 88, 180, 429, 622, 1201
BseDI CCNNGG 6 cut(s) 120, 260, 427, 620, 621, 1290
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 289, 1091
BseMI GCAATG 3 cut(s) 513, 963, 1062
BseMII CTCAG 2 cut(s) 111, 1117
BseNI ACTGG 2 cut(s) 391, 815
BseXI GCAGC 4 cut(s) 12, 457, 548, 995
BsgI GTGCAG 1 cut(s) 629
BshFI GGCC 1 cut(s) 618
BshNI GGYRCC 3 cut(s) 256, 279, 334
BsiSI CCGG 2 cut(s) 260, 962
BslFI GGGAC 2 cut(s) 189, 409
BslI CCNNNNNNNGG 2 cut(s) 289, 1091
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 2 cut(s) 189, 409
BsnI GGCC 1 cut(s) 618
Bsp143I GATC 4 cut(s) 17, 43, 408, 462
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 3 cut(s) 117, 1027, 1095
BspANI GGCC 1 cut(s) 618
BspCNI CTCAG 2 cut(s) 110, 1116
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 6 cut(s) 177, 258, 281, 336, 619, 1131
BspMAI CTGCAG 2 cut(s) 405, 538
BspPI GGATC 2 cut(s) 51, 403
BspT107I GGYRCC 3 cut(s) 256, 279, 334
BsrDI GCAATG 3 cut(s) 513, 963, 1062
BsrFI RCCGGY 1 cut(s) 961
BsrI ACTGG 2 cut(s) 391, 815
BssAI RCCGGY 1 cut(s) 961
BssECI CCNNGG 6 cut(s) 120, 260, 427, 620, 621, 1290
BssMI GATC 4 cut(s) 17, 43, 408, 462
BssT1I CCWWGG 2 cut(s) 120, 1290
Bst2UI CCWGG 5 cut(s) 88, 180, 429, 622, 1201
Bst4CI ACNGT 4 cut(s) 190, 421, 565, 851
Bst6I CTCTTC 1 cut(s) 260
BstBAI YACGTR 1 cut(s) 366
BstC8I GCNNGC 2 cut(s) 783, 1097
BstDEI CTNAG 4 cut(s) 97, 888, 1103, 1182
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 176
BstKTI GATC 4 cut(s) 20, 46, 411, 465
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 4 cut(s) 17, 43, 408, 462
BstMWI GCNNNNNNNGC 1 cut(s) 575
BstNI CCWGG 5 cut(s) 88, 180, 429, 622, 1201
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 6 cut(s) 86, 178, 259, 427, 620, 1199
BstSFI CTRYAG 3 cut(s) 401, 534, 561
BstV1I GCAGC 4 cut(s) 12, 457, 548, 995
BstV2I GAAGAC 1 cut(s) 137
BstX2I RGATCY 2 cut(s) 43, 408
BstYI RGATCY 2 cut(s) 43, 408
BsuRI GGCC 1 cut(s) 618
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 176
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 2 cut(s) 398, 856
Cac8I GCNNGC 2 cut(s) 783, 1097
CciI TCATGA 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 961
Cfr13I GGNCC 2 cut(s) 176, 617
CsiI ACCWGGT 1 cut(s) 86
Csp6I GTAC 4 cut(s) 94, 280, 335, 363
CviQI GTAC 4 cut(s) 94, 280, 335, 363
DdeI CTNAG 4 cut(s) 97, 888, 1103, 1182
DpnI GATC 4 cut(s) 19, 45, 410, 464
DpnII GATC 4 cut(s) 17, 43, 408, 462
DraIII CACNNNGTG 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 120, 1290
Eco47I GGWCC 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 5 cut(s) 86, 178, 427, 620, 1199
EcoT14I CCWWGG 2 cut(s) 120, 1290
EcoT22I ATGCAT 1 cut(s) 249
ErhI CCWWGG 2 cut(s) 120, 1290
FalI AAGNNNNNCTT 4 cut(s) 711, 743, 1178, 1210
FaqI GGGAC 2 cut(s) 189, 409
FauI CCCGC 1 cut(s) 1088
FauNDI CATATG 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 26, 471, 537, 984
FokI GGATG 1 cut(s) 183
Fsp4HI GCNGC 4 cut(s) 26, 471, 537, 984
FspBI CTAG 4 cut(s) 75, 317, 501, 989
GluI GCNGC 4 cut(s) 26, 471, 537, 984
GsuI CTGGAG 2 cut(s) 201, 1251
HaeIII GGCC 1 cut(s) 618
HapII CCGG 2 cut(s) 260, 962
HincII GTYRAC 1 cut(s) 296
HindII GTYRAC 1 cut(s) 296
HinfI GANTC 3 cut(s) 251, 715, 1074
HpaII CCGG 2 cut(s) 260, 962
HphI GGTGA 3 cut(s) 106, 205, 485
Hpy166II GTNNAC 2 cut(s) 296, 478
Hpy188I TCNGA 5 cut(s) 22, 48, 308, 845, 1073
Hpy188III TCNNGA 4 cut(s) 460, 857, 1038, 1311
Hpy8I GTNNAC 2 cut(s) 296, 478
HpyAV CCTTC 4 cut(s) 313, 741, 759, 763
HpyCH4III ACNGT 4 cut(s) 190, 421, 565, 851
HpyCH4IV ACGT 2 cut(s) 365, 1079
HpyF10VI GCNNNNNNNGC 1 cut(s) 575
HpyF3I CTNAG 4 cut(s) 97, 888, 1103, 1182
HpySE526I ACGT 2 cut(s) 365, 1079
KpnI GGTACC 2 cut(s) 283, 338
Kzo9I GATC 4 cut(s) 17, 43, 408, 462
LmnI GCTCC 4 cut(s) 575, 939, 1135, 1258
Lsp1109I GCAGC 4 cut(s) 12, 457, 548, 995
LweI GCATC 3 cut(s) 234, 378, 1164
MabI ACCWGGT 1 cut(s) 86
MaeI CTAG 4 cut(s) 75, 317, 501, 989
MaeII ACGT 2 cut(s) 365, 1079
MaeIII GTNAC 4 cut(s) 112, 184, 695, 1075
MalI GATC 4 cut(s) 19, 45, 410, 464
MboI GATC 4 cut(s) 17, 43, 408, 462
MboII GAAGA 5 cut(s) 137, 277, 418, 457, 1139
MfeI CAATTG 1 cut(s) 975
MflI RGATCY 2 cut(s) 43, 408
MluCI AATT 6 cut(s) 508, 910, 975, 1003, 1210, 1301
MlyI GAGTC 2 cut(s) 260, 1083
MnlI CCTC 5 cut(s) 261, 506, 878, 1109, 1263
Mph1103I ATGCAT 1 cut(s) 249
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 2 cut(s) 260, 962
MspR9I CCNGG 6 cut(s) 88, 180, 261, 429, 622, 1201
MunI CAATTG 1 cut(s) 975
MvaI CCWGG 5 cut(s) 88, 180, 429, 622, 1201
MwoI GCNNNNNNNGC 1 cut(s) 575
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 787
NdeII GATC 4 cut(s) 17, 43, 408, 462
NlaIV GGNNCC 6 cut(s) 177, 258, 281, 336, 619, 1131
NmuCI GTSAC 3 cut(s) 112, 695, 1075
NsiI ATGCAT 1 cut(s) 249
NspI RCATGY 1 cut(s) 302
PagI TCATGA 1 cut(s) 459
PasI CCCWGGG 1 cut(s) 621
PfeI GAWTC 1 cut(s) 715
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 4 cut(s) 27, 472, 538, 985
PleI GAGTC 2 cut(s) 259, 1082
PpsI GAGTC 2 cut(s) 259, 1082
Ppu21I YACGTR 1 cut(s) 366
PpuMI RGGWCCY 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 5 cut(s) 86, 178, 427, 620, 1199
PspGI CCWGG 5 cut(s) 86, 178, 427, 620, 1199
PspN4I GGNNCC 6 cut(s) 177, 258, 281, 336, 619, 1131
PspPI GGNCC 2 cut(s) 176, 617
PspPPI RGGWCCY 1 cut(s) 176
PstI CTGCAG 2 cut(s) 405, 538
PsuI RGATCY 2 cut(s) 43, 408
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 4 cut(s) 95, 281, 336, 364
RsaNI GTAC 4 cut(s) 94, 280, 335, 363
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 4 cut(s) 26, 471, 537, 984
Sau3AI GATC 4 cut(s) 17, 43, 408, 462
Sau96I GGNCC 2 cut(s) 176, 617
SchI GAGTC 2 cut(s) 260, 1083
ScrFI CCNGG 6 cut(s) 88, 180, 261, 429, 622, 1201
SexAI ACCWGGT 1 cut(s) 86
SfaNI GCATC 3 cut(s) 234, 378, 1164
SfcI CTRYAG 3 cut(s) 401, 534, 561
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 6 cut(s) 508, 910, 975, 1003, 1210, 1301
SsiI CCGC 3 cut(s) 117, 1027, 1095
SspMI CTAG 4 cut(s) 75, 317, 501, 989
StyD4I CCNGG 6 cut(s) 86, 178, 259, 427, 620, 1199
StyI CCWWGG 2 cut(s) 120, 1290
TaaI ACNGT 4 cut(s) 190, 421, 565, 851
TaiI ACGT 2 cut(s) 368, 1082
TaqI TCGA 1 cut(s) 629
TasI AATT 6 cut(s) 508, 910, 975, 1003, 1210, 1301
TfiI GAWTC 1 cut(s) 715
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 2 cut(s) 405, 856
TseFI GTSAC 3 cut(s) 112, 695, 1075
TseI GCWGC 4 cut(s) 25, 470, 536, 983
Tsp45I GTSAC 3 cut(s) 112, 695, 1075
TspDTI ATGAA 5 cut(s) 192, 445, 917, 1016, 1057
TspRI CASTG 2 cut(s) 405, 856
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 176
XapI RAATTY 2 cut(s) 1003, 1301
XceI RCATGY 1 cut(s) 302
XspI CTAG 4 cut(s) 75, 317, 501, 989
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.