Rorug06G0507300

Transmembrane amino acid transporter protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005950
Physical Location & Seq
Forward (+)
62224078 .. 62227280
3203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0507300.1

Sequence Viewer

Length: 1233 bp
ATGGCGGATGACAAAGGCCTTGAAGGCGACAAGAGCGAAGCCCCCGCCGCCGCCGCTACGCCTCCGGCGGTGCCGAAGAAGAATCGCAAGGGATTTTTTTCCAGAATGTTTACCGGCATGTTTCGGCACCGGGACGATTTCGAGAAGCGGCTGCAGTATCTCTCTAAGGAGGAGATTACTGTGGTCAATAGAATCAACAGGAGGGCTCTGACTCGGAGAAGCTTCACCAGAAACGTCATTGCATTCTCTATTTTTTTCGAGGTTATCGCAATTGGTTATGCTATAGCAACCACGAGAACGACTGACTTGGACTGGAGGACGAGGACATTTCGAATTTTGCCAATGTTTCTGTTGCCTGCTTTGTCTTCTTTTACTTATTCAACATTTATGAACTTCACAAGGATGCGTGATCGCAAAGATAATAAGACCCTGGAAAGGCTCCGGGCTGAAAAGCAAGCAAAAATTGATGAACTTAAAGAGAAGACAAACTATTACTCTACTCAACAGCTTATTCAGAAATATGATGCTGACCCAGCAGCAAAGGCAGCTGCTGCAACTGTCCTGGCATCTAAGTTGGGTGCGGATTCAGGCCTAAGATTCCATGTAGGAGATGAATCTAGAGCTAATGCCCCATCTGGGAGAAGCAAGGATGTTGAACTTGTCCAGTCTGGTGAACTTCGAAACCGGAAAACAGGACTCACCAGATCTAATAGTACAGGGAGTACACAATTGCTTTTTTCTGAGGAAGAGACTCCTTGTTCTGTTGGGTCTGAGGGTCCCCAGACTGCTGAGCATGGTCAGCTGGTTGTTGCTCATCAGAATCCTCAAGCATATGCTGCACAAGATGGAAATTGGATTGCTCGTATTGCAGCATTACTTGTGGGTGAGGATCCAACACAATCTTATGCCCTCATATGTGGCAACTGTCATATGCACAATGGGCTTGCTAAGAAGGAGGATTTCGCATACATTACTTATTATTGCCCACACTGTCATGCTCTAAATAGGCCAAAGCATTTGGAAGGGGGTGTTACTGGTTCAGGCTCTCCTGATTCGGGATCTCTGACAGCAGGGGACAGGAGTGATGTAGCAAACCAGGCCAGTGATTCTTTGAATGACAGTGTCGTCACAGGTACCAGCTCAGTTGTAGCTGGTCCTGAGATTGAGCAAATAACTGAGAGGCTTGTGTCGGGTGATACAGATACAGTTAGTTTAGAAGAAAAACACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

410

Amino Acids

45.17

Weight (kDa)

7.15

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_10 PF10058 285 - 335 3.8e-20 ER junction formation factor lunapark, zinc ribbon finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1124
Acc65I GGTACC 1 cut(s) 1133
AccB1I GGYRCC 3 cut(s) 70, 126, 1133
AciI CCGC 8 cut(s) 5, 45, 48, 51, 54, 68, 148, 581
AclWI GGATC 3 cut(s) 884, 897, 1066
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 3 cut(s) 715, 724, 1135
AfiI CCNNNNNNNGG 3 cut(s) 435, 636, 1055
AgsI TTSAA 4 cut(s) 23, 381, 656, 1114
AjnI CCWGG 3 cut(s) 429, 561, 1095
AluBI AGCT 7 cut(s) 222, 508, 548, 623, 802, 1140, 1151
AluI AGCT 7 cut(s) 222, 508, 548, 623, 802, 1140, 1151
Alw26I GTCTC 1 cut(s) 743
AlwI GGATC 3 cut(s) 884, 897, 1066
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 4 cut(s) 16, 589, 1007, 1098
ApeKI GCWGC 7 cut(s) 151, 536, 545, 548, 551, 836, 869
ApoI RAATTY 1 cut(s) 333
Asp718I GGTACC 1 cut(s) 1133
AspS9I GGNCC 2 cut(s) 776, 1154
AsuC2I CCSGG 2 cut(s) 131, 443
AsuHPI GGTGA 5 cut(s) 217, 683, 691, 896, 1205
AsuII TTCGAA 2 cut(s) 331, 679
AvaII GGWCC 2 cut(s) 776, 1154
BamHI GGATCC 1 cut(s) 889
BanI GGYRCC 3 cut(s) 70, 126, 1133
BanII GRGCYC 1 cut(s) 208
BauI CACGAG 2 cut(s) 292, 1226
BbsI GAAGAC 2 cut(s) 357, 488
BbvI GCAGC 7 cut(s) 138, 535, 538, 548, 557, 823, 881
BccI CCATC 2 cut(s) 640, 839
BciT130I CCWGG 3 cut(s) 431, 563, 1097
BcnI CCSGG 2 cut(s) 131, 443
BcoDI GTCTC 1 cut(s) 743
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 2 cut(s) 152, 282
BglI GCCNNNNNGGC 1 cut(s) 24
BglII AGATCT 1 cut(s) 704
BlpI GCTNAGC 1 cut(s) 789
Bme1390I CCNGG 5 cut(s) 131, 431, 443, 563, 1097
Bme18I GGWCC 2 cut(s) 776, 1154
BmgT120I GGNCC 2 cut(s) 776, 1154
BmiI GGNNCC 7 cut(s) 72, 128, 440, 777, 778, 891, 1135
BmrFI CCNGG 5 cut(s) 131, 431, 443, 563, 1097
BmsI GCATC 3 cut(s) 393, 514, 575
BpiI GAAGAC 2 cut(s) 357, 488
BpmI CTGGAG 1 cut(s) 334
Bpu1102I GCTNAGC 1 cut(s) 789
Bpu14I TTCGAA 2 cut(s) 331, 679
BpuEI CTTGAG 1 cut(s) 810
BpuMI CCSGG 2 cut(s) 131, 443
BsaJI CCNNGG 1 cut(s) 429
BsaWI WCCGGW 1 cut(s) 684
Bsc4I CCNNNNNNNGG 3 cut(s) 435, 636, 1055
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 4 cut(s) 317, 664, 1039, 1101
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 3 cut(s) 431, 563, 1097
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 3 cut(s) 13, 408, 655
BseLI CCNNNNNNNGG 3 cut(s) 435, 636, 1055
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 6 cut(s) 732, 762, 780, 1149, 1155, 1167
BseNI ACTGG 4 cut(s) 317, 664, 1039, 1101
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 7 cut(s) 138, 535, 538, 548, 557, 823, 881
BseYI CCCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 4 cut(s) 18, 591, 1009, 1100
BshNI GGYRCC 3 cut(s) 70, 126, 1133
BsiSI CCGG 5 cut(s) 65, 114, 130, 442, 685
BslFI GGGAC 3 cut(s) 146, 762, 1088
BslI CCNNNNNNNGG 3 cut(s) 435, 636, 1055
BsmAI GTCTC 1 cut(s) 743
BsmFI GGGAC 3 cut(s) 146, 762, 1088
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 4 cut(s) 18, 591, 1009, 1100
Bsp119I TTCGAA 2 cut(s) 331, 679
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 4 cut(s) 409, 704, 889, 1058
Bsp1720I GCTNAGC 1 cut(s) 789
BspACI CCGC 8 cut(s) 5, 45, 48, 51, 54, 68, 148, 581
BspANI GGCC 4 cut(s) 18, 591, 1009, 1100
BspCNI CTCAG 6 cut(s) 733, 763, 781, 1150, 1154, 1168
BspLI GGNNCC 7 cut(s) 72, 128, 440, 777, 778, 891, 1135
BspMAI CTGCAG 1 cut(s) 156
BspPI GGATC 3 cut(s) 884, 897, 1066
BspT104I TTCGAA 2 cut(s) 331, 679
BspT107I GGYRCC 3 cut(s) 70, 126, 1133
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 4 cut(s) 317, 664, 1039, 1101
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 4 cut(s) 409, 704, 889, 1058
BssSI CACGAG 2 cut(s) 292, 1226
Bst2BI CACGAG 2 cut(s) 292, 1226
Bst2UI CCWGG 3 cut(s) 431, 563, 1097
Bst4CI ACNGT 6 cut(s) 181, 559, 926, 992, 1121, 1207
Bst6I CTCTTC 1 cut(s) 741
BstAPI GCANNNNNTGC 2 cut(s) 551, 836
BstBI TTCGAA 2 cut(s) 331, 679
BstC8I GCNNGC 3 cut(s) 357, 456, 945
BstF5I GGATG 3 cut(s) 13, 408, 655
BstKTI GATC 4 cut(s) 412, 707, 892, 1061
BstMAI GTCTC 1 cut(s) 743
BstMBI GATC 4 cut(s) 409, 704, 889, 1058
BstNI CCWGG 3 cut(s) 431, 563, 1097
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 5 cut(s) 129, 429, 441, 561, 1095
BstSFI CTRYAG 2 cut(s) 152, 282
BstV1I GCAGC 7 cut(s) 138, 535, 538, 548, 557, 823, 881
BstV2I GAAGAC 2 cut(s) 357, 488
BstX2I RGATCY 3 cut(s) 704, 889, 1058
BstYI RGATCY 3 cut(s) 704, 889, 1058
BsuRI GGCC 4 cut(s) 18, 591, 1009, 1100
BtsCI GGATG 3 cut(s) 13, 408, 655
BtsIMutI CAGTG 3 cut(s) 988, 1108, 1126
Cac8I GCNNGC 3 cut(s) 357, 456, 945
CaiI CAGNNNCTG 1 cut(s) 551
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 776, 1154
Csp6I GTAC 3 cut(s) 714, 723, 1134
CviAII CATG 4 cut(s) 118, 602, 794, 995
CviQI GTAC 3 cut(s) 714, 723, 1134
DpnI GATC 4 cut(s) 411, 706, 891, 1060
DpnII GATC 4 cut(s) 409, 704, 889, 1058
DrdI GACNNNNNNGTC 1 cut(s) 1124
DseDI GACNNNNNNGTC 1 cut(s) 1124
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 2 cut(s) 18, 591
Eco24I GRGCYC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 776, 1154
EcoO109I RGGNCCY 1 cut(s) 776
EcoRII CCWGG 3 cut(s) 429, 561, 1095
EcoT38I GRGCYC 1 cut(s) 208
FaeI CATG 4 cut(s) 121, 605, 797, 998
FaqI GGGAC 3 cut(s) 146, 762, 1088
FatI CATG 4 cut(s) 117, 601, 793, 994
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 3 cut(s) 832, 914, 930
FokI GGATG 3 cut(s) 20, 415, 662
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 1 cut(s) 618
GsaI CCCAGC 1 cut(s) 536
GsuI CTGGAG 1 cut(s) 334
HaeIII GGCC 4 cut(s) 18, 591, 1009, 1100
HapII CCGG 5 cut(s) 65, 114, 130, 442, 685
Hin1II CATG 4 cut(s) 121, 605, 797, 998
HindIII AAGCTT 1 cut(s) 220
HpaII CCGG 5 cut(s) 65, 114, 130, 442, 685
HphI GGTGA 5 cut(s) 217, 683, 691, 896, 1205
Hpy166II GTNNAC 3 cut(s) 111, 674, 725
Hpy188I TCNGA 7 cut(s) 210, 216, 516, 742, 772, 819, 1065
Hpy188III TCNNGA 6 cut(s) 102, 142, 618, 1049, 1056, 1157
Hpy8I GTNNAC 3 cut(s) 111, 674, 725
HpyAV CCTTC 3 cut(s) 17, 946, 1016
HpyCH4III ACNGT 6 cut(s) 181, 559, 926, 992, 1121, 1207
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 6 cut(s) 154, 242, 554, 839, 869, 934
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 4 cut(s) 121, 605, 797, 998
KflI GGGWCCC 1 cut(s) 776
KpnI GGTACC 1 cut(s) 1137
Kzo9I GATC 4 cut(s) 409, 704, 889, 1058
LmnI GCTCC 1 cut(s) 444
Lsp1109I GCAGC 7 cut(s) 138, 535, 538, 548, 557, 823, 881
LweI GCATC 3 cut(s) 393, 514, 575
MaeI CTAG 1 cut(s) 618
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 1030, 1126
MalI GATC 4 cut(s) 411, 706, 891, 1060
MboI GATC 4 cut(s) 409, 704, 889, 1058
MboII GAAGA 6 cut(s) 88, 91, 357, 493, 758, 1229
MfeI CAATTG 2 cut(s) 270, 728
MflI RGATCY 3 cut(s) 704, 889, 1058
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 5 cut(s) 270, 333, 462, 728, 850
MlyI GAGTC 3 cut(s) 205, 690, 745
MmeI TCCRAC 1 cut(s) 917
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 2 cut(s) 401, 993
MspA1I CMGCKG 2 cut(s) 548, 802
MspI CCGG 5 cut(s) 65, 114, 130, 442, 685
MspR9I CCNGG 5 cut(s) 131, 431, 443, 563, 1097
MunI CAATTG 2 cut(s) 270, 728
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 3 cut(s) 431, 563, 1097
NciI CCSGG 2 cut(s) 131, 443
NdeI CATATG 3 cut(s) 832, 914, 930
NdeII GATC 4 cut(s) 409, 704, 889, 1058
NlaIII CATG 4 cut(s) 121, 605, 797, 998
NlaIV GGNNCC 7 cut(s) 72, 128, 440, 777, 778, 891, 1135
NmuCI GTSAC 1 cut(s) 1126
NspI RCATGY 1 cut(s) 121
NspV TTCGAA 2 cut(s) 331, 679
PceI AGGCCT 2 cut(s) 18, 591
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 8 cut(s) 82, 192, 584, 597, 614, 820, 1052, 1106
PflFI GACNNNGTC 1 cut(s) 1121
PleI GAGTC 3 cut(s) 205, 690, 745
PpsI GAGTC 3 cut(s) 205, 690, 745
PpuMI RGGWCCY 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 3 cut(s) 429, 561, 1095
PspFI CCCAGC 1 cut(s) 532
PspGI CCWGG 3 cut(s) 429, 561, 1095
PspN4I GGNNCC 7 cut(s) 72, 128, 440, 777, 778, 891, 1135
PspPI GGNCC 2 cut(s) 776, 1154
PspPPI RGGWCCY 1 cut(s) 776
PstI CTGCAG 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 3 cut(s) 704, 889, 1058
PsyI GACNNNGTC 1 cut(s) 1121
PvuII CAGCTG 2 cut(s) 548, 802
RsaI GTAC 3 cut(s) 715, 724, 1135
RsaNI GTAC 3 cut(s) 714, 723, 1134
RseI CAYNNNNRTG 2 cut(s) 401, 993
SaqAI TTAA 1 cut(s) 474
Sau3AI GATC 4 cut(s) 409, 704, 889, 1058
Sau96I GGNCC 2 cut(s) 776, 1154
SchI GAGTC 3 cut(s) 205, 690, 745
ScrFI CCNGG 5 cut(s) 131, 431, 443, 563, 1097
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 3 cut(s) 393, 514, 575
SfcI CTRYAG 2 cut(s) 152, 282
SfuI TTCGAA 2 cut(s) 331, 679
SinI GGWCC 2 cut(s) 776, 1154
SmiMI CAYNNNNRTG 2 cut(s) 401, 993
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
Sse9I AATT 5 cut(s) 270, 333, 462, 728, 850
SseBI AGGCCT 2 cut(s) 18, 591
SsiI CCGC 8 cut(s) 5, 45, 48, 51, 54, 68, 148, 581
SspMI CTAG 1 cut(s) 618
StuI AGGCCT 2 cut(s) 18, 591
StyD4I CCNGG 5 cut(s) 129, 429, 441, 561, 1095
TaaI ACNGT 6 cut(s) 181, 559, 926, 992, 1121, 1207
TaiI ACGT 1 cut(s) 237
TaqI TCGA 4 cut(s) 141, 258, 331, 679
TasI AATT 5 cut(s) 270, 333, 462, 728, 850
TatI WGTACW 2 cut(s) 713, 722
TauI GCSGC 4 cut(s) 50, 53, 56, 151
TfiI GAWTC 8 cut(s) 82, 192, 584, 597, 614, 820, 1052, 1106
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 3 cut(s) 995, 1108, 1126
TseFI GTSAC 1 cut(s) 1126
TseI GCWGC 7 cut(s) 151, 536, 545, 548, 551, 836, 869
Tsp45I GTSAC 1 cut(s) 1126
TspDTI ATGAA 3 cut(s) 404, 483, 627
TspRI CASTG 3 cut(s) 995, 1108, 1126
Tth111I GACNNNGTC 1 cut(s) 1121
VpaK11BI GGWCC 2 cut(s) 776, 1154
XapI RAATTY 1 cut(s) 333
XbaI TCTAGA 1 cut(s) 617
XceI RCATGY 1 cut(s) 121
XspI CTAG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.