Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009792

Physical Location & Seq

Genomic Coordinates Reverse (-)

30215143 .. 30218198

Gene Structure 3056 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1161200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGCTCCAGCTATCATCGCTCTTTCGAAGCCAATTCTTCATCAGTTTGCGAGGCTCAGAATGGAAGGAGAAAGCGGAGAATCTGGAAATGGAACTGCAGCAGTGTTACAAAGCTCAATCGCGGTTGTCCGAGCAACTCGTGGTGGAAGTAGCAGAGTCCAGAGCTTCAAAAGCTTCACTTCAAGAGAAAGAAGCGGCAGTGGCTGATCTTCAGAAGGAGTTAACTGAAAAAAGGGATGAATGCTCTCAGTTAGCGGCTGACTTGGAAGAGAAGATTAAAGCTTTAGAATTGGTTGTGAATGAGAACAAGGAAATAAGAGCACAGCTAGAAGCAATGTCTATTAGAGCTAGGAATGCTGAGACTGAAAGTAAGGTGTTAATTGACCGCTGGATGTTGGAAAAGATGAAGGATGCAGAACGCCTTAACGAGGCAAATGCACTATATGAAGATATGATTGAGCGGCTTAGGGCCAGCGGTTTGGAAAAACTTGCTAGGCAGCAGGTAGATGGTGTTGTCCGCCAAAGTGAAGAAGGTGCTGAGTACTATGTGGAGTCAACCATTCCCTCTGCATGCAAGAATAGGATCCCTGCCCATGAAGGAGGTTGTGCATCCATATTATTTGAGTACAATTCTGGCAGATTGATTACTGGTGGACAGGATGGGTCCATTAAAATGTGGGATACAAATAATGGGTCACTAACTCATACACTTCACGGTTGCATTGGTTCTGTTCTGGACCTGACGATTACCCATGACAATAAATCTATCATTGCAGCAAGCAGCTCAAACAAATTGTATGCCTGGGATGTTAGCTCAGGGAGGGTTCGCCACACTCTCACTGGTCACATGGATAAAGTGTGTGCAGTAGATGTCAGCAAATTCTCAAGTCGTCATGTTGTGAGTGCAGCTTATGATCGTACCATAAAAGTTTGGGATTTGCAGAAAGGTTACTGCACCAATACAATCATGTACCCGAAAAATTGCAATGCTTTGTCATTCAGCATGGATGGACAGACCATATGTTCAGGCCATGTTGATGGAAATCTTCGTTTGTGGGATATTGAGAAAGGAAAGCTACTCAGTGAAGTAGCTGCACATTCAAATGCTGTGACATCTATATCTTTGTCCCGAGATGGCAATGTTATACTGACCAGTGGGAGAGACAATGTGCATAATTTGTTTGATATGCGGTCTCTGGAAGTCCGCCGTACATTAAGAGCCACTGGAAACAGAGTAGCGTCGAATTGGAGCCGGTCCTGTATCAGTCCAGATGACAATTACGTTGCTGCTGGATCTGCTGACGGTTCTGTGTATATTTGGTCAATATCGAAAGCTGACATTGTGAGCACTCTGAAGGAGCACACTGCATCTGTCCTGTGTTGTTCATGGAGCGGGCATGGAAAACCTCTAGCTTCTGCGGACAAGAACGGAATCATCTGTACTTGGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000421 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005776 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006897 GO:0006907 GO:0006909 GO:0006914 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016740 GO:0019538 GO:0019776 GO:0019941 GO:0022607 GO:0030163 GO:0030587 GO:0031090 GO:0031154 GO:0032502 GO:0032991 GO:0034274 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0051179 GO:0051234 GO:0051603 GO:0051703 GO:0051704 GO:0061919 GO:0070925 GO:0071704 GO:0071840 GO:0090382 GO:0090702 GO:0098588 GO:0098657 GO:0098805 GO:0099120 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905037 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04621 map04136 map04138 map04140 map04621

Pfam Domains

Protein Families

ATG16 WD40

Protein Analysis

484

Amino Acids

53.53

Weight (kDa)

6.11

Isoelectric Point (pI)

40.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG16	PF08614	23 - 142	5.1e-12	Autophagy protein 16 (ATG16)
Beta-prop_WDR36-Utp21_2nd	PF25168	184 - 346	7.2e-17	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	190 - 327	1.1e-23	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	191 - 271	1.1e-09	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	191 - 279	1e-11	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	192 - 314	4e-11	TEP-1 second beta-propeller
WD40_Gbeta	PF25391	195 - 289	4.2e-08	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	197 - 480	1.3e-30	WDHD1 first WD40 domain
WDR55	PF24796	197 - 397	6.2e-20	WDR55
Beta-prop_EML_2	PF23414	199 - 295	9e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	200 - 315	7.4e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	201 - 324	5.2e-22	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	203 - 410	1.4e-31	Prp19 WD40 domain
Beta-prop_ACSF4	PF13570	209 - 456	2e-10	Beta-alanine-activating enzyme, beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	210 - 313	6.2e-08	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	252 - 440	7e-18	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st	PF25171	253 - 390	1.9e-14	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	260 - 481	4.8e-36	THOC3 beta-propeller domain
NBCH_WD40	PF20426	270 - 445	1.1e-09	Neurobeachin beta propeller domain
WD40	PF00400	274 - 312	2.2e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	276 - 395	6.7e-13	WDR3 second beta-propeller domain
EIF3I	PF24805	278 - 340	1e-07	EIF3I
Beta-prop_SCAP	PF24017	291 - 450	3.3e-07	SCAP Beta-propeller
WD40_CDC20-Fz	PF24807	294 - 467	1.8e-21	CDC20/Fizzy WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	295 - 415	4.1e-11	WDR90/POC16, second beta-propeller
WDR90_beta-prop_4th	PF23342	295 - 391	3e-06	WDR90, 4th beta-propeller
Beta-prop_EML_2	PF23414	296 - 407	7.3e-13	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	300 - 482	1.5e-13	Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_1st	PF24780	326 - 428	2e-06	MABP1/WDR62 first WD40 domain
ANAPC4_WD40	PF12894	326 - 374	3.8e-06	Anaphase-promoting complex subunit 4 WD40 domain
EIF3I	PF24805	328 - 428	5.7e-12	EIF3I
Beta-prop_WDR3_1st	PF25173	328 - 413	9.1e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	330 - 412	4.6e-16	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	330 - 402	7.1e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	338 - 474	1e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	361 - 446	2.2e-07	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	416 - 482	3.4e-10	WDR3 first beta-propeller domain
WDR90_beta-prop_4th	PF23342	416 - 480	4.4e-08	WDR90, 4th beta-propeller
Beta-prop_WDR5	PF25175	420 - 482	2.7e-09	WDR5 beta-propeller domain
WD40_Prp19	PF24814	421 - 482	1.3e-06	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	421 - 482	6.6e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT140_1st	PF23383	423 - 482	2.4e-07	IFT140 first beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009792)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50230
fragaria_vesca	FvH4_5g06790 FvH4_5g06790
malus_domestica	MD06G1161200.v1.1 MD14G1166400.v1.1
prunus_persica	Prupe.5G160700_v2.0.a1 Prupe.5G160700_v2.0.a1
pyrus_communis	pycom06g14390 pycom14g13900
rosa_chinensis	RchiOBHm_Chr7g0191761 RchiOBHm_Chr7g0191771
rosa_laevigata	RLG00000004427
rosa_multiflora	Rmu_co8375695.1_g000001
rosa_roxburghii	Rroxscaffold_3G00263550
rosa_rugosa	Rorug06G0511200
rosa_samantha	Rh7AG121000 Rh7BG122700 Rh7CG125600 Rh7DG124300
rosa_wichuraiana	Rw7G010300 Rw7G010310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	490
AccBSI	CCGCTC	2 cut(s)	460, 1392
AccII	CGCG	1 cut(s)	121
AclWI	GGATC	3 cut(s)	577, 590, 1300
AcsI	RAATTY	1 cut(s)	878
AcuI	CTGAAG	2 cut(s)	194, 1373
AfaI	GTAC	6 cut(s)	542, 626, 919, 971, 1210, 1441
AfiI	CCNNNNNNNGG	1 cut(s)	427
AgsI	TTSAA	3 cut(s)	168, 182, 1101
AjiI	CACGTC	1 cut(s)	1449
AjnI	CCWGG	1 cut(s)	800
Alw21I	GWGCWC	3 cut(s)	322, 1349, 1362
Alw26I	GTCTC	3 cut(s)	353, 1155, 1197
AlwI	GGATC	3 cut(s)	577, 590, 1300
AlwNI	CAGNNNCTG	1 cut(s)	203
Ama87I	CYCGRG	1 cut(s)	1128
AoxI	GGCC	2 cut(s)	468, 1027
ApeKI	GCWGC	7 cut(s)	97, 496, 773, 780, 905, 1091, 1286
ApoI	RAATTY	1 cut(s)	878
ArsI	GACNNNNNNTTYG	2 cut(s)	677, 709
AspS9I	GGNCC	4 cut(s)	468, 663, 736, 1254
AsuII	TTCGAA	1 cut(s)	25
AvaI	CYCGRG	1 cut(s)	1128
AvaII	GGWCC	3 cut(s)	663, 736, 1254
BamHI	GGATCC	1 cut(s)	582
BauI	CACGAG	1 cut(s)	137
Bbv12I	GWGCWC	3 cut(s)	322, 1349, 1362
BbvI	GCAGC	7 cut(s)	109, 508, 785, 792, 917, 1078, 1273
BccI	CCATC	5 cut(s)	500, 653, 1001, 1031, 1127
BceAI	ACGGC	1 cut(s)	1191
BcgI	CGANNNNNNTGC	2 cut(s)	416, 450
BciT130I	CCWGG	1 cut(s)	802
BciVI	GTATCC	1 cut(s)	673
BcoDI	GTCTC	3 cut(s)	353, 1155, 1197
BfaI	CTAG	4 cut(s)	326, 348, 492, 1409
BfmI	CTRYAG	1 cut(s)	95
BfuAI	ACCTGC	1 cut(s)	490
BfuI	GTATCC	1 cut(s)	673
BmcAI	AGTACT	1 cut(s)	542
Bme1390I	CCNGG	1 cut(s)	802
Bme18I	GGWCC	3 cut(s)	663, 736, 1254
BmeT110I	CYCGRG	1 cut(s)	1128
BmgBI	CACGTC	1 cut(s)	1449
BmgT120I	GGNCC	4 cut(s)	468, 663, 736, 1254
BmiI	GGNNCC	3 cut(s)	584, 664, 1250
BmrFI	CCNGG	1 cut(s)	802
BmsI	GCATC	3 cut(s)	400, 617, 1376
Bpu10I	CCTNAGC	2 cut(s)	464, 814
Bpu14I	TTCGAA	1 cut(s)	25
BpuEI	CTTGAG	1 cut(s)	868
BsaBI	GATNNNNATC	1 cut(s)	1041
BsaI	GGTCTC	1 cut(s)	1197
BsaJI	CCNNGG	1 cut(s)	801
Bsc4I	CCNNNNNNNGG	1 cut(s)	427
Bse118I	RCCGGY	1 cut(s)	1251
Bse1I	ACTGG	4 cut(s)	652, 844, 1152, 1228
Bse3DI	GCAATG	4 cut(s)	339, 768, 991, 1144
Bse8I	GATNNNNATC	1 cut(s)	1041
BseBI	CCWGG	1 cut(s)	802
BseDI	CCNNGG	1 cut(s)	801
BseGI	GGATG	7 cut(s)	241, 396, 415, 608, 664, 811, 1012
BseJI	GATNNNNATC	1 cut(s)	1041
BseLI	CCNNNNNNNGG	1 cut(s)	427
BseMI	GCAATG	4 cut(s)	339, 768, 991, 1144
BseMII	CTCAG	6 cut(s)	69, 260, 348, 528, 828, 1093
BseNI	ACTGG	4 cut(s)	652, 844, 1152, 1228
BseXI	GCAGC	7 cut(s)	109, 508, 785, 792, 917, 1078, 1273
BsgI	GTGCAG	4 cut(s)	882, 924, 937, 1077
Bsh1236I	CGCG	1 cut(s)	121
BshFI	GGCC	2 cut(s)	470, 1029
BsiHKAI	GWGCWC	3 cut(s)	322, 1349, 1362
BsiHKCI	CYCGRG	1 cut(s)	1128
BsiSI	CCGG	1 cut(s)	1252
BslFI	GGGAC	1 cut(s)	1111
BslI	CCNNNNNNNGG	1 cut(s)	427
BsmAI	GTCTC	3 cut(s)	353, 1155, 1197
BsmFI	GGGAC	1 cut(s)	1111
BsmI	GAATGC	2 cut(s)	245, 358
BsnI	GGCC	2 cut(s)	470, 1029
Bso31I	GGTCTC	1 cut(s)	1197
BsoBI	CYCGRG	1 cut(s)	1128
Bsp119I	TTCGAA	1 cut(s)	25
Bsp1286I	GDGCHC	3 cut(s)	322, 1349, 1362
Bsp143I	GATC	4 cut(s)	205, 582, 913, 1292
BspANI	GGCC	2 cut(s)	470, 1029
BspCNI	CTCAG	6 cut(s)	68, 259, 349, 529, 827, 1092
BspFNI	CGCG	1 cut(s)	121
BspLI	GGNNCC	3 cut(s)	584, 664, 1250
BspMAI	CTGCAG	1 cut(s)	99
BspMI	ACCTGC	1 cut(s)	490
BspPI	GGATC	3 cut(s)	577, 590, 1300
BspT104I	TTCGAA	1 cut(s)	25
BspTNI	GGTCTC	1 cut(s)	1197
BsrBI	CCGCTC	2 cut(s)	460, 1392
BsrDI	GCAATG	4 cut(s)	339, 768, 991, 1144
BsrFI	RCCGGY	1 cut(s)	1251
BsrI	ACTGG	4 cut(s)	652, 844, 1152, 1228
BssAI	RCCGGY	1 cut(s)	1251
BssECI	CCNNGG	1 cut(s)	801
BssMI	GATC	4 cut(s)	205, 582, 913, 1292
BssSI	CACGAG	1 cut(s)	137
Bst2BI	CACGAG	1 cut(s)	137
Bst2UI	CCWGG	1 cut(s)	802
Bst4CI	ACNGT	2 cut(s)	716, 1304
Bst6I	CTCTTC	1 cut(s)	261
BstBI	TTCGAA	1 cut(s)	25
BstC8I	GCNNGC	4 cut(s)	472, 571, 778, 1394
BstDEI	CTNAG	7 cut(s)	55, 246, 357, 464, 537, 814, 1079
BstENI	CCTNNNNNAGG	1 cut(s)	425
BstF5I	GGATG	7 cut(s)	241, 396, 415, 608, 664, 811, 1012
BstFNI	CGCG	1 cut(s)	121
BstKTI	GATC	4 cut(s)	208, 585, 916, 1295
BstMAI	GTCTC	3 cut(s)	353, 1155, 1197
BstMBI	GATC	4 cut(s)	205, 582, 913, 1292
BstMWI	GCNNNNNNNGC	5 cut(s)	16, 170, 200, 353, 1295
BstNI	CCWGG	1 cut(s)	802
BstNSI	RCATGY	1 cut(s)	573
BstSCI	CCNGG	1 cut(s)	800
BstSFI	CTRYAG	1 cut(s)	95
BstUI	CGCG	1 cut(s)	121
BstV1I	GCAGC	7 cut(s)	109, 508, 785, 792, 917, 1078, 1273
BstX2I	RGATCY	2 cut(s)	582, 1292
BstXI	CCANNNNNNTGG	2 cut(s)	478, 1037
BstYI	RGATCY	2 cut(s)	582, 1292
BsuI	GTATCC	1 cut(s)	673
BsuRI	GGCC	2 cut(s)	470, 1029
BtrI	CACGTC	1 cut(s)	1449
BtsCI	GGATG	7 cut(s)	241, 396, 415, 608, 664, 811, 1012
BtsI	GCAGTG	3 cut(s)	107, 204, 1362
BtsIMutI	CAGTG	7 cut(s)	107, 204, 837, 1087, 1159, 1221, 1362
BveI	ACCTGC	1 cut(s)	490
Cac8I	GCNNGC	4 cut(s)	472, 571, 778, 1394
CaiI	CAGNNNCTG	1 cut(s)	203
Cfr10I	RCCGGY	1 cut(s)	1251
Cfr13I	GGNCC	4 cut(s)	468, 663, 736, 1254
CseI	GACGC	1 cut(s)	1227
Csp6I	GTAC	6 cut(s)	541, 625, 918, 970, 1209, 1440
CviQI	GTAC	6 cut(s)	541, 625, 918, 970, 1209, 1440
DdeI	CTNAG	7 cut(s)	55, 246, 357, 464, 537, 814, 1079
DpnI	GATC	4 cut(s)	207, 584, 915, 1294
DpnII	GATC	4 cut(s)	205, 582, 913, 1292
Eam1104I	CTCTTC	1 cut(s)	261
EarI	CTCTTC	1 cut(s)	261
EciI	GGCGGA	2 cut(s)	506, 1193
Eco31I	GGTCTC	1 cut(s)	1197
Eco47I	GGWCC	3 cut(s)	663, 736, 1254
Eco57I	CTGAAG	2 cut(s)	194, 1373
Eco88I	CYCGRG	1 cut(s)	1128
EcoNI	CCTNNNNNAGG	1 cut(s)	425
EcoRII	CCWGG	1 cut(s)	800
FalI	AAGNNNNNCTT	2 cut(s)	162, 194
FaqI	GGGAC	1 cut(s)	1111
FauI	CCCGC	1 cut(s)	1385
FauNDI	CATATG	1 cut(s)	1019
FokI	GGATG	7 cut(s)	248, 403, 422, 595, 671, 818, 1019
FspBI	CTAG	4 cut(s)	326, 348, 492, 1409
HaeIII	GGCC	2 cut(s)	470, 1029
HapII	CCGG	1 cut(s)	1252
HgaI	GACGC	1 cut(s)	1227
HincII	GTYRAC	2 cut(s)	222, 555
HindII	GTYRAC	2 cut(s)	222, 555
HindIII	AAGCTT	2 cut(s)	171, 279
HinfI	GANTC	4 cut(s)	79, 155, 551, 1431
HpaI	GTTAAC	1 cut(s)	222
HpaII	CCGG	1 cut(s)	1252
Hpy166II	GTNNAC	3 cut(s)	222, 555, 653
Hpy188I	TCNGA	4 cut(s)	58, 130, 213, 1353
Hpy188III	TCNNGA	7 cut(s)	83, 159, 182, 734, 1128, 1196, 1268
Hpy8I	GTNNAC	3 cut(s)	222, 555, 653
Hpy99I	CGWCG	1 cut(s)	1243
HpyAV	CCTTC	6 cut(s)	58, 208, 400, 524, 590, 1348
HpyCH4III	ACNGT	2 cut(s)	716, 1304
HpyCH4IV	ACGT	2 cut(s)	1281, 1448
HpyF10VI	GCNNNNNNNGC	5 cut(s)	16, 170, 200, 353, 1295
HpyF3I	CTNAG	7 cut(s)	55, 246, 357, 464, 537, 814, 1079
HpySE526I	ACGT	2 cut(s)	1281, 1448
KspAI	GTTAAC	1 cut(s)	222
Kzo9I	GATC	4 cut(s)	205, 582, 913, 1292
LmnI	GCTCC	4 cut(s)	9, 1248, 1357, 1389
Lsp1109I	GCAGC	7 cut(s)	109, 508, 785, 792, 917, 1078, 1273
LweI	GCATC	3 cut(s)	400, 617, 1376
MaeI	CTAG	4 cut(s)	326, 348, 492, 1409
MaeII	ACGT	2 cut(s)	1281, 1448
MaeIII	GTNAC	5 cut(s)	104, 693, 842, 947, 1108
MalI	GATC	4 cut(s)	207, 584, 915, 1294
MbiI	CCGCTC	2 cut(s)	460, 1392
MboI	GATC	4 cut(s)	205, 582, 913, 1292
MboII	GAAGA	7 cut(s)	28, 200, 278, 283, 458, 539, 1037
MflI	RGATCY	2 cut(s)	582, 1292
MhlI	GDGCHC	3 cut(s)	322, 1349, 1362
MlyI	GAGTC	2 cut(s)	164, 560
MmeI	TCCRAC	1 cut(s)	375
MnlI	CCTC	6 cut(s)	44, 421, 574, 593, 813, 1416
MseI	TTAA	6 cut(s)	221, 276, 377, 423, 669, 1214
MslI	CAYNNNNRTG	3 cut(s)	671, 1035, 1101
MspA1I	CMGCKG	2 cut(s)	387, 474
MspI	CCGG	1 cut(s)	1252
MspR9I	CCNGG	1 cut(s)	802
Mva1269I	GAATGC	2 cut(s)	245, 358
MvaI	CCWGG	1 cut(s)	802
MvnI	CGCG	1 cut(s)	121
MwoI	GCNNNNNNNGC	5 cut(s)	16, 170, 200, 353, 1295
NdeI	CATATG	1 cut(s)	1019
NdeII	GATC	4 cut(s)	205, 582, 913, 1292
NlaIV	GGNNCC	3 cut(s)	584, 664, 1250
NmuCI	GTSAC	3 cut(s)	693, 842, 1108
NspI	RCATGY	1 cut(s)	573
NspV	TTCGAA	1 cut(s)	25
PaeI	GCATGC	1 cut(s)	573
PctI	GAATGC	2 cut(s)	245, 358
PfeI	GAWTC	2 cut(s)	79, 1431
PleI	GAGTC	2 cut(s)	163, 559
PpsI	GAGTC	2 cut(s)	163, 559
Psp6I	CCWGG	1 cut(s)	800
PspGI	CCWGG	1 cut(s)	800
PspN4I	GGNNCC	3 cut(s)	584, 664, 1250
PspPI	GGNCC	4 cut(s)	468, 663, 736, 1254
PstI	CTGCAG	1 cut(s)	99
PstNI	CAGNNNCTG	1 cut(s)	203
PsuI	RGATCY	2 cut(s)	582, 1292
RsaI	GTAC	6 cut(s)	542, 626, 919, 971, 1210, 1441
RsaNI	GTAC	6 cut(s)	541, 625, 918, 970, 1209, 1440
RseI	CAYNNNNRTG	3 cut(s)	671, 1035, 1101
SaqAI	TTAA	6 cut(s)	221, 276, 377, 423, 669, 1214
Sau3AI	GATC	4 cut(s)	205, 582, 913, 1292
Sau96I	GGNCC	4 cut(s)	468, 663, 736, 1254
ScaI	AGTACT	1 cut(s)	542
SchI	GAGTC	2 cut(s)	164, 560
ScrFI	CCNGG	1 cut(s)	802
SduI	GDGCHC	3 cut(s)	322, 1349, 1362
SfaNI	GCATC	3 cut(s)	400, 617, 1376
SfcI	CTRYAG	1 cut(s)	95
SfuI	TTCGAA	1 cut(s)	25
SinI	GGWCC	3 cut(s)	663, 736, 1254
SmiMI	CAYNNNNRTG	3 cut(s)	671, 1035, 1101
SmlI	CTYRAG	1 cut(s)	883
SmoI	CTYRAG	1 cut(s)	883
SphI	GCATGC	1 cut(s)	573
SspMI	CTAG	4 cut(s)	326, 348, 492, 1409
StyD4I	CCNGG	1 cut(s)	800
TaaI	ACNGT	2 cut(s)	716, 1304
TaiI	ACGT	2 cut(s)	1284, 1451
TaqI	TCGA	3 cut(s)	25, 1241, 1328
TatI	WGTACW	3 cut(s)	540, 624, 1439
TauI	GCSGC	3 cut(s)	197, 257, 463
TfiI	GAWTC	2 cut(s)	79, 1431
Tru1I	TTAA	6 cut(s)	221, 276, 377, 423, 669, 1214
Tru9I	TTAA	6 cut(s)	221, 276, 377, 423, 669, 1214
TscAI	CASTG	7 cut(s)	107, 204, 844, 1087, 1159, 1228, 1369
TseFI	GTSAC	3 cut(s)	693, 842, 1108
TseI	GCWGC	7 cut(s)	97, 496, 773, 780, 905, 1091, 1286
Tsp45I	GTSAC	3 cut(s)	693, 842, 1108
TspDTI	ATGAA	6 cut(s)	28, 252, 419, 459, 609, 1374
TspGWI	ACGGA	1 cut(s)	1443
TspRI	CASTG	7 cut(s)	107, 204, 844, 1087, 1159, 1228, 1369
VpaK11BI	GGWCC	3 cut(s)	663, 736, 1254
XagI	CCTNNNNNAGG	1 cut(s)	425
XapI	RAATTY	1 cut(s)	878
XceI	RCATGY	1 cut(s)	573
XcmI	CCANNNNNNNNNTGG	1 cut(s)	836
XspI	CTAG	4 cut(s)	326, 348, 492, 1409
ZrmI	AGTACT	1 cut(s)	542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.