MD14G1166400.v1.1

Autophagy protein 16 (ATG16)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009792
Physical Location & Seq
Forward (+)
25983717 .. 25986811
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1166400.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGAATTTTCTTGGCTGCCAAACAGAGGCGATCGAGGAGATTGCGAGGGAAGCAATAAAACACGCTCTGAAGGCTCTTCGGCGGCGGCATTTGGCGGAAGAAGGTGCTCATGCTCCAGCTTTCATCGCTCTTTCGAAGCCAATTCTTCATCAGGGCTCAGAATGGAAGGAGAAAGCGGAGAATCTGGAAATGGAACTTCAGCAGTGTTACAAAGCCCAATCTCGGCTGTCCGAGCAACTCGTGGTGGAAGTAGCGGAGTCCAGAACTTCAAAAGCTTCACTTCAAGAGAAAGAGGCAGCAGTGGCTAATCTTCAGAAGGAATTATCTGAACAAAGGGATGAATGCTCTCAATTAGCGGCTGACTTGGAAGAGAAGGTTAAAGCTTTGGAATTGGTTGTGAGTGAGAACAAGGAAATAAGAGCGCAGCTAGAAGAAATGTCTATTCGAGCTAAGAACGCTGAGGCCGAAAATAAGGTGTTAATTGACCGCTGGATGTTGGAGAAGATGAAGGATGCAGAACGCCTTAACGAGGCAAATGCACTCTATGAAGATATGATTGAGCGGCTGAGGGCCAGCGGTTTGGAGAAACTTGCTAGGCAGCAAGTAGATGGTGTTGTCCGCCAAAGTGAAGAAGGTGCTGAGTACTTTTTGGAGTCAACCATTCCCTCCACATGCAAGAATAGGATGCCTGCCCATGATGGTGGCTGCGCTTCCATATTATTTGAGTACAATTCAGGGAAATTGATTACTGGTGGACAGGATGGGTCCATCAAAATGTGGGATACAAATACTGGGTCACTAACTCGTACACTCTATGGTTGCATTGGTTCTGTTCTGGATCTCACGATTACTCATGATAATAGATCTATCATTGCAGCAAGCAGCTCTAACAAATTGTATGCCTGGGATGTTAACTCGGGGAGGGTTCGACATACTCTCACTGGCCACATGGATAAAGTGTGTGCAGTAGATGTCAGCAAATTCTCAAGCCGCCATGTTGTGAGTGCAGCTTATGATCGTACCATAAAAGTTTGGGATATGCAGAAAGGTTACTGCACCAACACAATCATGTTCCCAAAAAACTGCAATGCTCTGTCATTTAGCATGGATGGACAGACCATATGTTCTGGCCATGTTGATGGAAATCTTCGTTTGTGGGATATTGAGAAAGGAAAACTCCTCAGTGAAGTAGCTGCACACTCAAATGCTGTTACATCCATATCTTTGTCCCGAAATGGCAATGTTATACTGACCAGTGGGAGAGACAATGTCCATAATTTGTTTGACATGCGGTCTCTAGAAGTCTGTGGAACATTAAGAGCCACAGGAAACAGAGTAGCGTCGAATTGGAGTAGATCCTGTATCAGTCCAGATGACAATTACGTTGCTGCTGGATCTGCTGACGGTTGTGTGTATATTTGGTCAATATCGAAAGCTGACATTGTGAGCACTATGAAGGAGCACACTGCATCGGTTCTGTGTTGTTCATGGAGCGGGCATGGAAAACCTCTGGCTTCTGCGGACAAGAACGGAATCATCTGTACTTGGACGTGA

Protein Analysis

518

Amino Acids

57.13

Weight (kDa)

6.02

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG16 PF08614 53 - 176 1.9e-14 Autophagy protein 16 (ATG16)
Beta-prop_WDR36-Utp21_2nd PF25168 217 - 380 1.7e-16 WDR36/Utp21 second beta-propeller domain
Beta-prop_SCAP PF24017 223 - 307 4.4e-06 SCAP Beta-propeller
Beta-prop_WDR5 PF25175 224 - 359 8.4e-24 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 225 - 303 1.4e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 225 - 313 1.6e-11 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 226 - 348 9.1e-11 TEP-1 second beta-propeller
WD40_Gbeta PF25391 229 - 323 4.1e-09 G protein beta WD-40 repeat protein
WDR55 PF24796 229 - 431 1.4e-18 WDR55
WD40_WDHD1_1st PF24817 231 - 514 3.8e-30 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 233 - 349 3.8e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 236 - 357 4e-21 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 239 - 444 1e-31 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 243 - 346 1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_ACSF4 PF13570 247 - 514 5.6e-10 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_THOC3 PF25174 287 - 515 4.9e-37 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 290 - 424 1.4e-13 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 291 - 429 7.5e-11 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_SCAP PF24017 303 - 485 1.4e-07 SCAP Beta-propeller
NBCH_WD40 PF20426 304 - 479 3.1e-08 Neurobeachin beta propeller domain
EIF3I PF24805 306 - 374 2.7e-08 EIF3I
WD40 PF00400 308 - 346 2.4e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 309 - 429 3.1e-13 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 328 - 501 7.4e-22 CDC20/Fizzy WD40 domain
WDR90_beta-prop_4th PF23342 329 - 424 9.1e-06 WDR90, 4th beta-propeller
Beta-prop_EML_2 PF23414 330 - 441 1.4e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 330 - 474 1.1e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 330 - 449 6.8e-11 WDR90/POC16, second beta-propeller
Beta-prop_EML PF23409 336 - 516 1.7e-12 Echinoderm microtubule-associated protein first beta-propeller
WDR55 PF24796 358 - 516 2.6e-14 WDR55
WD40_MABP1-WDR62_1st PF24780 360 - 431 4.3e-06 MABP1/WDR62 first WD40 domain
ANAPC4_WD40 PF12894 361 - 409 4.3e-06 Anaphase-promoting complex subunit 4 WD40 domain
EIF3I PF24805 362 - 462 3.6e-11 EIF3I
Beta-prop_WDR3_1st PF25173 362 - 447 8.9e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 364 - 446 2.8e-15 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 364 - 436 6e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 373 - 508 4.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 395 - 480 9.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 428 - 516 4.9e-10 WDR3 first beta-propeller domain
WDR90_beta-prop_4th PF23342 450 - 514 1.7e-08 WDR90, 4th beta-propeller
Beta-prop_WDR5 PF25175 454 - 516 6.4e-09 WDR5 beta-propeller domain
WD40_Prp19 PF24814 455 - 516 2.7e-06 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 455 - 516 3.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT140_1st PF23383 457 - 516 6.6e-07 IFT140 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 562, 1494
AclWI GGATC 3 cut(s) 846, 1350, 1402
AcoI YGGCCR 2 cut(s) 943, 1129
AcsI RAATTY 2 cut(s) 4, 980
AcuI CTGAAG 3 cut(s) 89, 182, 296
AfaI GTAC 5 cut(s) 644, 728, 808, 1021, 1543
AfiI CCNNNNNNNGG 3 cut(s) 25, 222, 529
AgsI TTSAA 2 cut(s) 270, 284
AhdI GACNNNNNGTC 1 cut(s) 1291
AjiI CACGTC 1 cut(s) 1551
AjnI CCWGG 1 cut(s) 902
AluBI AGCT 9 cut(s) 119, 275, 383, 427, 449, 885, 1010, 1193, 1436
AluI AGCT 9 cut(s) 119, 275, 383, 427, 449, 885, 1010, 1193, 1436
Alw21I GWGCWC 3 cut(s) 109, 1451, 1464
Alw26I GTCTC 2 cut(s) 1257, 1299
AlwI GGATC 3 cut(s) 846, 1350, 1402
Ama87I CYCGRG 1 cut(s) 916
AoxI GGCC 4 cut(s) 462, 570, 943, 1129
ApoI RAATTY 2 cut(s) 4, 980
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AspLEI GCGC 2 cut(s) 424, 710
AspS9I GGNCC 2 cut(s) 570, 765
AsuII TTCGAA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 916
AvaII GGWCC 1 cut(s) 765
BalI TGGCCA 2 cut(s) 945, 1131
BanII GRGCYC 1 cut(s) 158
BauI CACGAG 1 cut(s) 239
Bbv12I GWGCWC 3 cut(s) 109, 1451, 1464
BbvCI CCTCAGC 2 cut(s) 459, 566
BccI CCATC 6 cut(s) 602, 692, 755, 776, 1103, 1133
BcgI CGANNNNNNTGC 4 cut(s) 23, 57, 518, 552
BciT130I CCWGG 1 cut(s) 904
BciVI GTATCC 1 cut(s) 775
BcoDI GTCTC 2 cut(s) 1257, 1299
BfaI CTAG 3 cut(s) 428, 594, 1298
BfuI GTATCC 1 cut(s) 775
BglII AGATCT 1 cut(s) 863
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 1 cut(s) 904
Bme18I GGWCC 1 cut(s) 765
BmeRI GACNNNNNGTC 1 cut(s) 1291
BmeT110I CYCGRG 1 cut(s) 916
BmgBI CACGTC 1 cut(s) 1551
BmgT120I GGNCC 2 cut(s) 570, 765
BmiI GGNNCC 1 cut(s) 766
BmrFI CCNGG 1 cut(s) 904
BmrI ACTGGG 1 cut(s) 801
BmsI GCATC 3 cut(s) 502, 675, 1478
BmuI ACTGGG 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 99
Bpu10I CCTNAGC 2 cut(s) 459, 566
Bpu14I TTCGAA 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 970
BsaBI GATNNNNATC 1 cut(s) 1143
BsaI GGTCTC 1 cut(s) 1299
BsaJI CCNNGG 1 cut(s) 903
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 222, 529
Bse1I ACTGG 4 cut(s) 754, 796, 946, 1254
Bse3DI GCAATG 3 cut(s) 870, 1093, 1246
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 1 cut(s) 904
BseDI CCNNGG 1 cut(s) 903
BseGI GGATG 8 cut(s) 343, 498, 517, 690, 766, 913, 1114, 1214
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 3 cut(s) 25, 222, 529
BseMI GCAATG 3 cut(s) 870, 1093, 1246
BseMII CTCAG 5 cut(s) 171, 450, 557, 630, 1195
BseNI ACTGG 4 cut(s) 754, 796, 946, 1254
BseRI GAGGAG 2 cut(s) 50, 1169
BsgI GTGCAG 4 cut(s) 984, 1026, 1039, 1179
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 4 cut(s) 464, 572, 945, 1131
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 3 cut(s) 109, 1451, 1464
BsiHKCI CYCGRG 1 cut(s) 916
BslFI GGGAC 1 cut(s) 1213
BslI CCNNNNNNNGG 3 cut(s) 25, 222, 529
BsmAI GTCTC 2 cut(s) 1257, 1299
BsmFI GGGAC 1 cut(s) 1213
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 4 cut(s) 464, 572, 945, 1131
Bso31I GGTCTC 1 cut(s) 1299
BsoBI CYCGRG 1 cut(s) 916
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 4 cut(s) 109, 158, 1451, 1464
Bsp143I GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
BspANI GGCC 4 cut(s) 464, 572, 945, 1131
BspCNI CTCAG 5 cut(s) 170, 451, 558, 631, 1194
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 1 cut(s) 766
BspPI GGATC 3 cut(s) 846, 1350, 1402
BspQI GCTCTTC 1 cut(s) 81
BspT104I TTCGAA 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 1299
BsrBI CCGCTC 2 cut(s) 562, 1494
BsrDI GCAATG 3 cut(s) 870, 1093, 1246
BsrI ACTGG 4 cut(s) 754, 796, 946, 1254
BssECI CCNNGG 1 cut(s) 903
BssMI GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
BssSI CACGAG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 904
Bst4CI ACNGT 1 cut(s) 1406
Bst6I CTCTTC 2 cut(s) 81, 363
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 4 cut(s) 574, 690, 880, 1496
BstDEI CTNAG 6 cut(s) 157, 450, 459, 566, 639, 1181
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 8 cut(s) 343, 498, 517, 690, 766, 913, 1114, 1214
BstHHI GCGC 2 cut(s) 424, 710
BstKTI GATC 6 cut(s) 33, 841, 866, 1018, 1358, 1397
BstMAI GTCTC 2 cut(s) 1257, 1299
BstMBI GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 7 cut(s) 50, 71, 125, 232, 302, 455, 1397
BstNI CCWGG 1 cut(s) 904
BstNSI RCATGY 2 cut(s) 675, 1291
BstSCI CCNGG 1 cut(s) 902
BstX2I RGATCY 4 cut(s) 838, 863, 1355, 1394
BstXI CCANNNNNNTGG 3 cut(s) 580, 701, 1139
BstYI RGATCY 4 cut(s) 838, 863, 1355, 1394
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 4 cut(s) 464, 572, 945, 1131
BtgZI GCGATG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 1551
BtsCI GGATG 8 cut(s) 343, 498, 517, 690, 766, 913, 1114, 1214
BtsI GCAGTG 3 cut(s) 209, 306, 1464
BtsIMutI CAGTG 6 cut(s) 209, 306, 939, 1189, 1261, 1464
Cac8I GCNNGC 4 cut(s) 574, 690, 880, 1496
CciI TCATGA 1 cut(s) 853
CfoI GCGC 2 cut(s) 424, 710
Cfr13I GGNCC 2 cut(s) 570, 765
CseI GACGC 1 cut(s) 1329
Csp6I GTAC 5 cut(s) 643, 727, 807, 1020, 1542
CviQI GTAC 5 cut(s) 643, 727, 807, 1020, 1542
DdeI CTNAG 6 cut(s) 157, 450, 459, 566, 639, 1181
DpnI GATC 6 cut(s) 32, 840, 865, 1017, 1357, 1396
DpnII GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
DriI GACNNNNNGTC 1 cut(s) 1291
EaeI YGGCCR 2 cut(s) 943, 1129
Eam1104I CTCTTC 2 cut(s) 81, 363
Eam1105I GACNNNNNGTC 1 cut(s) 1291
EarI CTCTTC 2 cut(s) 81, 363
EciI GGCGGA 2 cut(s) 110, 608
Eco24I GRGCYC 1 cut(s) 158
Eco31I GGTCTC 1 cut(s) 1299
Eco47I GGWCC 1 cut(s) 765
Eco57I CTGAAG 3 cut(s) 89, 182, 296
Eco88I CYCGRG 1 cut(s) 916
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoRII CCWGG 1 cut(s) 902
EcoT38I GRGCYC 1 cut(s) 158
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 1213
FauI CCCGC 1 cut(s) 1487
FauNDI CATATG 1 cut(s) 1121
FokI GGATG 8 cut(s) 350, 505, 524, 697, 773, 920, 1121, 1201
FriOI GRGCYC 1 cut(s) 158
FspBI CTAG 3 cut(s) 428, 594, 1298
GlaI GCGC 2 cut(s) 423, 709
GsuI CTGGAG 1 cut(s) 99
HaeIII GGCC 4 cut(s) 464, 572, 945, 1131
HgaI GACGC 1 cut(s) 1329
HhaI GCGC 2 cut(s) 424, 710
Hin6I GCGC 2 cut(s) 422, 708
HinP1I GCGC 2 cut(s) 422, 708
HincII GTYRAC 2 cut(s) 657, 913
HindII GTYRAC 2 cut(s) 657, 913
HindIII AAGCTT 2 cut(s) 273, 381
HinfI GANTC 4 cut(s) 181, 257, 653, 1533
HpaI GTTAAC 1 cut(s) 913
Hpy166II GTNNAC 4 cut(s) 657, 755, 809, 913
Hpy188I TCNGA 5 cut(s) 69, 160, 232, 315, 328
Hpy188III TCNNGA 9 cut(s) 185, 261, 284, 836, 844, 854, 1230, 1298, 1370
Hpy8I GTNNAC 4 cut(s) 657, 755, 809, 913
Hpy99I CGWCG 1 cut(s) 1345
HpyAV CCTTC 8 cut(s) 64, 95, 160, 310, 367, 502, 626, 1450
HpyCH4III ACNGT 1 cut(s) 1406
HpyCH4IV ACGT 2 cut(s) 1383, 1550
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 71, 125, 232, 302, 455, 1397
HpyF3I CTNAG 6 cut(s) 157, 450, 459, 566, 639, 1181
HpySE526I ACGT 2 cut(s) 1383, 1550
HspAI GCGC 2 cut(s) 422, 708
KspAI GTTAAC 1 cut(s) 913
Kzo9I GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 3 cut(s) 118, 1459, 1491
LweI GCATC 3 cut(s) 502, 675, 1478
MaeI CTAG 3 cut(s) 428, 594, 1298
MaeII ACGT 2 cut(s) 1383, 1550
MaeIII GTNAC 4 cut(s) 206, 795, 1049, 1210
MalI GATC 6 cut(s) 32, 840, 865, 1017, 1357, 1396
MbiI CCGCTC 2 cut(s) 562, 1494
MboI GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
MflI RGATCY 4 cut(s) 838, 863, 1355, 1394
MhlI GDGCHC 4 cut(s) 109, 158, 1451, 1464
MlsI TGGCCA 2 cut(s) 945, 1131
MluNI TGGCCA 2 cut(s) 945, 1131
MlyI GAGTC 2 cut(s) 266, 662
MmeI TCCRAC 1 cut(s) 477
Mox20I TGGCCA 2 cut(s) 945, 1131
MscI TGGCCA 2 cut(s) 945, 1131
MseI TTAA 5 cut(s) 378, 479, 525, 912, 1316
MslI CAYNNNNRTG 5 cut(s) 699, 773, 1067, 1137, 1203
Msp20I TGGCCA 2 cut(s) 945, 1131
MspA1I CMGCKG 2 cut(s) 489, 576
MspR9I CCNGG 1 cut(s) 904
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 1 cut(s) 904
MwoI GCNNNNNNNGC 7 cut(s) 50, 71, 125, 232, 302, 455, 1397
NdeI CATATG 1 cut(s) 1121
NdeII GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
NlaIV GGNNCC 1 cut(s) 766
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 795
NspI RCATGY 2 cut(s) 675, 1291
NspV TTCGAA 1 cut(s) 134
PagI TCATGA 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PctI GAATGC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 181, 1533
PflFI GACNNNGTC 1 cut(s) 1268
Ple19I CGATCG 1 cut(s) 33
PleI GAGTC 2 cut(s) 265, 661
PpsI GAGTC 2 cut(s) 265, 661
Psp6I CCWGG 1 cut(s) 902
PspGI CCWGG 1 cut(s) 902
PspN4I GGNNCC 1 cut(s) 766
PspPI GGNCC 2 cut(s) 570, 765
PsuI RGATCY 4 cut(s) 838, 863, 1355, 1394
PsyI GACNNNGTC 1 cut(s) 1268
PvuI CGATCG 1 cut(s) 33
RsaI GTAC 5 cut(s) 644, 728, 808, 1021, 1543
RsaNI GTAC 5 cut(s) 643, 727, 807, 1020, 1542
RseI CAYNNNNRTG 5 cut(s) 699, 773, 1067, 1137, 1203
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 5 cut(s) 378, 479, 525, 912, 1316
Sau3AI GATC 6 cut(s) 30, 838, 863, 1015, 1355, 1394
Sau96I GGNCC 2 cut(s) 570, 765
ScaI AGTACT 1 cut(s) 644
SchI GAGTC 2 cut(s) 266, 662
ScrFI CCNGG 1 cut(s) 904
SduI GDGCHC 4 cut(s) 109, 158, 1451, 1464
SfaNI GCATC 3 cut(s) 502, 675, 1478
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 1 cut(s) 765
SmiMI CAYNNNNRTG 5 cut(s) 699, 773, 1067, 1137, 1203
SmlI CTYRAG 1 cut(s) 985
SmoI CTYRAG 1 cut(s) 985
SspMI CTAG 3 cut(s) 428, 594, 1298
StyD4I CCNGG 1 cut(s) 902
TaaI ACNGT 1 cut(s) 1406
TaiI ACGT 2 cut(s) 1386, 1553
TaqI TCGA 6 cut(s) 33, 134, 445, 928, 1343, 1430
TatI WGTACW 3 cut(s) 642, 726, 1541
TauI GCSGC 5 cut(s) 85, 88, 359, 565, 993
TfiI GAWTC 2 cut(s) 181, 1533
Tru1I TTAA 5 cut(s) 378, 479, 525, 912, 1316
Tru9I TTAA 5 cut(s) 378, 479, 525, 912, 1316
TscAI CASTG 6 cut(s) 209, 306, 946, 1189, 1261, 1471
TseFI GTSAC 1 cut(s) 795
Tsp45I GTSAC 1 cut(s) 795
TspDTI ATGAA 8 cut(s) 17, 112, 137, 354, 521, 561, 1469, 1476
TspGWI ACGGA 1 cut(s) 1545
TspRI CASTG 6 cut(s) 209, 306, 946, 1189, 1261, 1471
Tth111I GACNNNGTC 1 cut(s) 1268
VpaK11BI GGWCC 1 cut(s) 765
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 2 cut(s) 4, 980
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 2 cut(s) 675, 1291
XspI CTAG 3 cut(s) 428, 594, 1298
ZrmI AGTACT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.