Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0009792

Physical Location & Seq

Genomic Coordinates Reverse (-)

59260607 .. 59269801

Gene Structure 9195 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004427

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2670 bp

ATGGGTGGGGTTACTTCATCCATGGCTGCAAAGCTTGCGTTTTTCCCACCAAACCCACCCTCCTACAAGGTCATTAAGGACCAGGCAACTGGGCTCTTGCTCATCGACCCTTTTCCCCACCGTGAAAACGTCGACGTTTTGAAGTTTCAGACCCGCCGCGGCAATGAGATCGTGGCCGTTTATGTCCGCCACCCAATGGCCACCTCCACCGTTCTGTATTCTCATGGCAACGCTACTGATATAGGCCAGATGTTTGAGCTCTTCATTGAATTGAGTATTCACCTGCGTGTCAATCTCATGGGGTATGATTACTCTGGATATGGGCAGTCATCAGGAAAGCCAAGTGAACATAATACTTATGCAGATATTGAAGCTGCATATAAGTGTCTCGAAGAGAGGTATGGTGCCAAGCAGGAGGACATAATCCTCTATGGTCAATCTGTCGGAAGTGGCCCTACTGTTGATCTTGCTGCTCGTTTGCCTCAACTGAGAGCAATTGTTCTGCATAGTCCGATATTATCAGGGTTAAGAGTTATGTATTCTGTGAAGCGCAGTTATTGGTTTGATATCTACAAGAATATTGATAAAATCCCACTGGTGAAATGTCCAGTGCTGGTAATACATGGAACATGTGATGAAGTAGTTGACTGCTCTCACGGCAAGCAACTTTGGCTACTGTGCCAAGAGAAATACGAACCTCTATGGCTCAAAGGTGGAAATCACTGCAATTTGGAACTCTATCCGGAATATATTAGACATCTCAACAAGTTCATATCCACTGTTGAAAAAGCGCCTCCACGCAAGTTGAGTTCAAGGAAGAGCACAGACTGTGTCGAACGTCCTAGGCGTAGTGTTGAATATCCTAGGCTCAGTGTTGATTACTCTGAGCTTCCGAGGAAGAGTACTGATAAGAGAGAAAAATCAAGGAAAAGCACCGATAGACGACCTGAAAAGCTGAGACTTAATGATTACAAGTTAAATAACAGTGACAAGCTAGAGAAGTTTAGGATCTCTGTTGATCAGATAGAGAGGTCCCGAAGGAGCGTCGAATACCACCATGAGAGATCATCTAGGAGAAGCGTTGACCATCAGCTAGAAAAACCAAGGAGAAGTGTCGATTGGCTGGACAGAATTCGAGCTGGGTCGCAGGAGGAGGTAGCTAGGAAGGCGATTAAGCACGCGCTGAAGGCACTAAGGAAGCGGCATTTGCTTGAAGAAGGTGCTCATGCTCCAGCGTATATCGCTCTCGCCAGTCCAATTGCTCATCAGGGCTCTGAATGGAAGGAAAAGGCTGAGAATTTGGAACTGGAGCTTCAGCAATGCTATAAAGCTCAATCTCGGTTGTCGGAGCAGCTTGTGGTCGAAGTAGCAGAGTCCAGGTGTTCGAAAGCTTTACTTCAGGAGAAAGAAGCAGCGATTGCGGATCAGCAGAATGAGCTGACACAAAAAAGGGATGAGTGCTCTCAGTTGAAGGCTGAGTTAGAAAAGAAGATTGAAGCTCTAGAGTTGGTTATGAGTGAGAATAATGAAATACGAGCGCAGCTAGAAGAAATGTCTGTTAGAGCTAAGAATGCTGAAGCTGAAAATAAGGTGTTAATTGACCGCTGGATGTTGGAAAAGATGAAGGATGCTGAACGCCTAAATGAGGCAAATGCACTATATGAAGAAATGCTTGAGCGGCTCAAGGCCAGTGGTTTGCAAAAACTAGCCAAGCAGCAAGTAGATGGTGTGGTCCGCCAAAGTGAAGAAGGTGCTGAGTACTTTGTGGAGTCAACCAATCCCACCACATGCAAGAACAGGATCAATGCCCATGACGGTGGCTGTGCTTCCATATTATTCGAGTACAACTCGGGCAAATTGATTTCTGGTGGACAGGATGGGACAATCAAAATGTGGGATACAAGTACAGGGGCTTTGAATAATACACTTCATGGTTGCATTGGTTCTGTTCTTGACCTTACCATTACCCATGATAATAGATCTATCATCGCAGCAAGCAGTTCGAACAAATTGTATGTATGGGATGCTAACTTGGGGAGAGTGCGCCATACTCTCACCGGCCACATGGATAAAGTTTGTGCTGTAGATGTGAGTAAGTTCTCAAGTCGTCACATTGTGAGTGCAGCCTACGATCGTACTATAAAAGTCTGGGATTTGCAAAAAGGTTACTGCACCAACACAATAATCTTCCCCAAACACTGCAATGCTCTTTGCTTCAGCATGGACGGGCAGACCATTTGCTCAGGTCATGTTGATGGGAATCTTCGGCTGTGGGATATTCAGAAAGGAAAGCTACTCAACGAAGTGGCTGCACACTCAAATACTGTTACGTCTATATCTTTGTCGCGAAGTGGCAACATGTTATTGACCAGTGGGAGGGACAATGTGCATCACCTTTTCGATATGAGAACCCTAGAAGTTTGTGGCACATTGAGAGCCACCGGAAACAGAGTAGCATCTAATTGGAGCCGGTCCTGTATCAGTCCAGATGACAATTATGTTGCTGCTGGATCTGCCGATGGTGCTGTGTATATCTGGTCTATAGCCAAAGGTGACATTGTGAGCACTCTGAAGGAACACACTAATTCTGTCCTCTGCTGCTCATGGAGTGGACATGGAAAACCTCTAGCTTCTGCTGACAAGAATGGAGTAATTTGTACTTGGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000421 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005776 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006897 GO:0006907 GO:0006909 GO:0006914 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016740 GO:0019538 GO:0019776 GO:0019941 GO:0022607 GO:0030163 GO:0030587 GO:0031090 GO:0031154 GO:0032502 GO:0032991 GO:0034274 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0051179 GO:0051234 GO:0051603 GO:0051703 GO:0051704 GO:0061919 GO:0070925 GO:0071704 GO:0071840 GO:0090382 GO:0090702 GO:0098588 GO:0098657 GO:0098805 GO:0099120 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905037 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04621 map04136 map04138 map04140 map04621

Pfam Domains

Protein Families

ATG16 WD40

Protein Analysis

890

Amino Acids

100.08

Weight (kDa)

8.49

Isoelectric Point (pI)

42.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	69 - 174	1.9e-07	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	71 - 171	8.3e-06	alpha/beta hydrolase fold
ATG16	PF08614	423 - 548	2.7e-11	Autophagy protein 16 (ATG16)
Beta-prop_WDR5	PF25175	593 - 729	1.5e-23	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	594 - 685	1.6e-11	THOC3 beta-propeller domain
WDR55	PF24796	595 - 803	9.1e-18	WDR55
WD40_CDC20-Fz	PF24807	595 - 795	1e-20	CDC20/Fizzy WD40 domain
Beta-prop_SCAP	PF24017	596 - 677	8.9e-06	SCAP Beta-propeller
Beta-prop_TEP1_2nd	PF25047	596 - 721	4.2e-09	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	597 - 675	3e-09	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	601 - 695	2.2e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	601 - 752	1.4e-15	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	601 - 729	7.3e-21	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	603 - 886	1.2e-29	WDHD1 first WD40 domain
WD40_Prp19	PF24814	607 - 816	1.2e-29	Prp19 WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	616 - 719	6.5e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	643 - 720	2.2e-07	WDR3 second beta-propeller domain
Beta-prop_ACSF4	PF13570	654 - 886	7.9e-08	Beta-alanine-activating enzyme, beta-propeller
WD40_Gbeta	PF25391	658 - 846	4.4e-19	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	660 - 746	6.7e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	662 - 796	5e-11	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	663 - 801	1.4e-11	Echinoderm microtubule-associated protein first beta-propeller
EIF3I	PF24805	663 - 746	3.5e-08	EIF3I
Beta-prop_THOC3	PF25174	664 - 887	1.5e-36	THOC3 beta-propeller domain
NBCH_WD40	PF20426	680 - 850	3.6e-08	Neurobeachin beta propeller domain
WD40	PF00400	680 - 718	3.4e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	683 - 801	4.6e-12	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	702 - 814	1.9e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	702 - 821	3.6e-10	WDR90/POC16, second beta-propeller
WDR55	PF24796	728 - 888	1.7e-13	WDR55
WD40_CDC20-Fz	PF24807	733 - 873	9.9e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	734 - 820	3.4e-12	WDR3 first beta-propeller domain
EIF3I	PF24805	734 - 834	1.5e-11	EIF3I
Beta-prop_WDR5	PF25175	735 - 818	3e-15	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	736 - 803	2.2e-07	MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	736 - 808	5.1e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	744 - 881	1.4e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	768 - 852	1.7e-06	TEP-1 second beta-propeller
WDR90_beta-prop_4th	PF23342	822 - 886	3e-06	WDR90, 4th beta-propeller
Beta-prop_WDR3_1st	PF25173	824 - 888	6.7e-10	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	826 - 888	7.3e-10	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	827 - 888	2.3e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	828 - 888	3.1e-06	Prp19 WD40 domain
Beta-prop_IFT140_1st	PF23383	829 - 888	1.4e-06	IFT140 first beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009792)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50230
fragaria_vesca	FvH4_5g06790 FvH4_5g06790
malus_domestica	MD06G1161200.v1.1 MD14G1166400.v1.1
prunus_persica	Prupe.5G160700_v2.0.a1 Prupe.5G160700_v2.0.a1
pyrus_communis	pycom06g14390 pycom14g13900
rosa_chinensis	RchiOBHm_Chr7g0191761 RchiOBHm_Chr7g0191771
rosa_laevigata	RLG00000004427
rosa_multiflora	Rmu_co8375695.1_g000001
rosa_roxburghii	Rroxscaffold_3G00263550
rosa_rugosa	Rorug06G0511200
rosa_samantha	Rh7AG121000 Rh7BG122700 Rh7CG125600 Rh7DG124300
rosa_wichuraiana	Rw7G010300 Rw7G010310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	291
Acc36I	ACCTGC	1 cut(s)	291
AccB1I	GGYRCC	1 cut(s)	404
AccB7I	CCANNNNNTGG	1 cut(s)	196
AccBSI	CCGCTC	1 cut(s)	1678
AccI	GTMKAC	1 cut(s)	132
AccII	CGCG	3 cut(s)	159, 1181, 2347
AccIII	TCCGGA	1 cut(s)	742
AciI	CCGC	9 cut(s)	154, 157, 159, 187, 1201, 1421, 1603, 1678, 1735
AclWI	GGATC	4 cut(s)	1016, 1431, 1808, 2518
AcoI	YGGCCR	3 cut(s)	174, 198, 2059
AcsI	RAATTY	2 cut(s)	1131, 1297
AcuI	CTGAAG	6 cut(s)	1205, 1298, 1382, 1596, 2200, 2591
AdeI	CACNNNGTG	1 cut(s)	2116
AfaI	GTAC	6 cut(s)	904, 1760, 1844, 1906, 2137, 2659
AfiI	CCNNNNNNNGG	3 cut(s)	196, 1645, 2376
AflIII	ACRYGT	2 cut(s)	629, 2358
AjiI	CACGTC	1 cut(s)	2667
AjnI	CCWGG	2 cut(s)	81, 1376
AjuI	GAANNNNNNNTTGG	2 cut(s)	1102, 1134
AleI	CACNNNNGTG	1 cut(s)	285
Alw21I	GWGCWC	5 cut(s)	261, 824, 1225, 1463, 2567
Alw26I	GTCTC	2 cut(s)	392, 952
AlwI	GGATC	4 cut(s)	1016, 1431, 1808, 2518
Ama87I	CYCGRG	1 cut(s)	1849
Aor13HI	TCCGGA	1 cut(s)	742
AoxI	GGCC	6 cut(s)	174, 198, 244, 451, 1686, 2059
ApoI	RAATTY	2 cut(s)	1131, 1297
AspA2I	CCTAGG	2 cut(s)	842, 863
AspLEI	GCGC	5 cut(s)	552, 793, 1183, 1540, 2046
AspS9I	GGNCC	5 cut(s)	79, 452, 1032, 1732, 2472
AsuHPI	GGTGA	5 cut(s)	272, 610, 2047, 2384, 2564
AsuII	TTCGAA	2 cut(s)	1385, 2003
AvaI	CYCGRG	1 cut(s)	1849
AvaII	GGWCC	4 cut(s)	79, 1032, 1732, 2472
AvrII	CCTAGG	2 cut(s)	842, 863
BalI	TGGCCA	1 cut(s)	200
BanI	GGYRCC	1 cut(s)	404
BanII	GRGCYC	3 cut(s)	96, 261, 1274
BarI	GAAGNNNNNNTAC	2 cut(s)	439, 471
Bbv12I	GWGCWC	5 cut(s)	261, 824, 1225, 1463, 2567
BccI	CCATC	5 cut(s)	1095, 1718, 1871, 2249, 2513
BceAI	ACGGC	2 cut(s)	161, 673
BcgI	CGANNNNNNTGC	6 cut(s)	151, 185, 1983, 2017, 2291, 2325
BciT130I	CCWGG	2 cut(s)	83, 1378
BciVI	GTATCC	1 cut(s)	1891
BclI	TGATCA	1 cut(s)	1018
BcoDI	GTCTC	2 cut(s)	392, 952
BfmI	CTRYAG	2 cut(s)	2082, 2541
BfoI	RGCGCY	1 cut(s)	794
BfuAI	ACCTGC	1 cut(s)	291
BfuI	GTATCC	1 cut(s)	1891
BglII	AGATCT	1 cut(s)	1979
BlnI	CCTAGG	2 cut(s)	842, 863
BmcAI	AGTACT	2 cut(s)	904, 1760
Bme1390I	CCNGG	2 cut(s)	83, 1378
Bme18I	GGWCC	4 cut(s)	79, 1032, 1732, 2472
BmeT110I	CYCGRG	1 cut(s)	1849
BmgBI	CACGTC	1 cut(s)	2667
BmgT120I	GGNCC	5 cut(s)	79, 452, 1032, 1732, 2472
BmiI	GGNNCC	3 cut(s)	406, 1034, 2468
BmrFI	CCNGG	2 cut(s)	83, 1378
BmrI	ACTGGG	1 cut(s)	99
BmsI	GCATC	4 cut(s)	1618, 2014, 2398, 2465
BmuI	ACTGGG	1 cut(s)	99
BplI	GAGNNNNNCTC	2 cut(s)	1832, 1864
BpmI	CTGGAG	2 cut(s)	1215, 1328
Bpu10I	CCTNAGC	1 cut(s)	2242
Bpu14I	TTCGAA	2 cut(s)	1385, 2003
BpuEI	CTTGAG	3 cut(s)	1667, 1694, 2086
BsaBI	GATNNNNATC	1 cut(s)	2259
BsaJI	CCNNGG	6 cut(s)	21, 157, 842, 863, 893, 1103
BsaWI	WCCGGW	2 cut(s)	742, 2441
BsaXI	ACNNNNNCTCC	2 cut(s)	44, 74
Bsc4I	CCNNNNNNNGG	3 cut(s)	196, 1645, 2376
Bse118I	RCCGGY	2 cut(s)	2057, 2469
Bse1I	ACTGG	7 cut(s)	94, 600, 608, 1251, 1311, 1689, 2370
Bse3DI	GCAATG	3 cut(s)	169, 1325, 2209
Bse8I	GATNNNNATC	1 cut(s)	2259
BseAI	TCCGGA	1 cut(s)	742
BseBI	CCWGG	2 cut(s)	83, 1378
BseDI	CCNNGG	6 cut(s)	21, 157, 842, 863, 893, 1103
BseGI	GGATG	6 cut(s)	17, 1459, 1614, 1633, 1882, 2029
BseJI	GATNNNNATC	1 cut(s)	2259
BseLI	CCNNNNNNNGG	3 cut(s)	196, 1645, 2376
BseMI	GCAATG	3 cut(s)	169, 1325, 2209
BseMII	CTCAG	9 cut(s)	479, 876, 883, 947, 1284, 1467, 1478, 1746, 2256
BseNI	ACTGG	7 cut(s)	94, 600, 608, 1251, 1311, 1689, 2370
BseRI	GAGGAG	1 cut(s)	1166
BseYI	CCCAGC	1 cut(s)	1139
BsgI	GTGCAG	3 cut(s)	2142, 2155, 2295
Bsh1236I	CGCG	3 cut(s)	159, 1181, 2347
Bsh1285I	CGRYCG	1 cut(s)	2134
BshFI	GGCC	6 cut(s)	176, 200, 246, 453, 1688, 2061
BshNI	GGYRCC	1 cut(s)	404
BsiEI	CGRYCG	1 cut(s)	2134
BsiHKAI	GWGCWC	5 cut(s)	261, 824, 1225, 1463, 2567
BsiHKCI	CYCGRG	1 cut(s)	1849
BsiSI	CCGG	4 cut(s)	743, 2058, 2442, 2470
BslFI	GGGAC	3 cut(s)	1018, 1894, 2393
BslI	CCNNNNNNNGG	3 cut(s)	196, 1645, 2376
BsmAI	GTCTC	2 cut(s)	392, 952
BsmFI	GGGAC	3 cut(s)	1018, 1894, 2393
BsmI	GAATGC	1 cut(s)	1576
BsnI	GGCC	6 cut(s)	176, 200, 246, 453, 1688, 2061
BsoBI	CYCGRG	1 cut(s)	1849
Bsp119I	TTCGAA	2 cut(s)	1385, 2003
Bsp1286I	GDGCHC	7 cut(s)	96, 261, 824, 1225, 1274, 1463, 2567
Bsp13I	TCCGGA	1 cut(s)	742
Bsp19I	CCATGG	1 cut(s)	21
Bsp68I	TCGCGA	1 cut(s)	2347
BspACI	CCGC	9 cut(s)	154, 157, 159, 187, 1201, 1421, 1603, 1678, 1735
BspANI	GGCC	6 cut(s)	176, 200, 246, 453, 1688, 2061
BspCNI	CTCAG	9 cut(s)	480, 877, 882, 948, 1285, 1468, 1477, 1747, 2255
BspEI	TCCGGA	1 cut(s)	742
BspFNI	CGCG	3 cut(s)	159, 1181, 2347
BspLI	GGNNCC	3 cut(s)	406, 1034, 2468
BspMI	ACCTGC	1 cut(s)	291
BspPI	GGATC	4 cut(s)	1016, 1431, 1808, 2518
BspQI	GCTCTTC	2 cut(s)	266, 812
BspT104I	TTCGAA	2 cut(s)	1385, 2003
BspT107I	GGYRCC	1 cut(s)	404
BsrBI	CCGCTC	1 cut(s)	1678
BsrDI	GCAATG	3 cut(s)	169, 1325, 2209
BsrFI	RCCGGY	2 cut(s)	2057, 2469
BsrI	ACTGG	7 cut(s)	94, 600, 608, 1251, 1311, 1689, 2370
BssAI	RCCGGY	2 cut(s)	2057, 2469
BssECI	CCNNGG	6 cut(s)	21, 157, 842, 863, 893, 1103
BssT1I	CCWWGG	4 cut(s)	21, 842, 863, 1103
Bst2UI	CCWGG	2 cut(s)	83, 1378
Bst4CI	ACNGT	9 cut(s)	122, 211, 460, 678, 781, 830, 986, 1817, 2326
Bst6I	CTCTTC	4 cut(s)	266, 387, 812, 893
BstAPI	GCANNNNNTGC	2 cut(s)	35, 1418
BstBI	TTCGAA	2 cut(s)	1385, 2003
BstC8I	GCNNGC	4 cut(s)	36, 662, 1179, 1996
BstDSI	CCRYGG	2 cut(s)	21, 157
BstENI	CCTNNNNNAGG	1 cut(s)	1643
BstF5I	GGATG	6 cut(s)	17, 1459, 1614, 1633, 1882, 2029
BstFNI	CGCG	3 cut(s)	159, 1181, 2347
BstH2I	RGCGCY	1 cut(s)	794
BstHHI	GCGC	5 cut(s)	552, 793, 1183, 1540, 2046
BstMAI	GTCTC	2 cut(s)	392, 952
BstMCI	CGRYCG	1 cut(s)	2134
BstNI	CCWGG	2 cut(s)	83, 1378
BstNSI	RCATGY	3 cut(s)	633, 1791, 2362
BstSCI	CCNGG	2 cut(s)	81, 1376
BstSFI	CTRYAG	2 cut(s)	2082, 2541
BstUI	CGCG	3 cut(s)	159, 1181, 2347
BstX2I	RGATCY	3 cut(s)	1008, 1979, 2510
BstXI	CCANNNNNNTGG	2 cut(s)	89, 1817
BstYI	RGATCY	3 cut(s)	1008, 1979, 2510
BsuI	GTATCC	1 cut(s)	1891
BsuRI	GGCC	6 cut(s)	176, 200, 246, 453, 1688, 2061
BtgI	CCRYGG	2 cut(s)	21, 157
BtgZI	GCGATG	1 cut(s)	1972
BtrI	CACGTC	1 cut(s)	2667
BtsCI	GGATG	6 cut(s)	17, 1459, 1614, 1633, 1882, 2029
BtsI	GCAGTG	2 cut(s)	721, 2197
BtsIMutI	CAGTG	9 cut(s)	593, 615, 721, 777, 877, 991, 1696, 2197, 2377
BtuMI	TCGCGA	1 cut(s)	2347
BveI	ACCTGC	1 cut(s)	291
Cac8I	GCNNGC	4 cut(s)	36, 662, 1179, 1996
CfoI	GCGC	5 cut(s)	552, 793, 1183, 1540, 2046
Cfr10I	RCCGGY	2 cut(s)	2057, 2469
Cfr13I	GGNCC	5 cut(s)	79, 452, 1032, 1732, 2472
Cfr42I	CCGCGG	1 cut(s)	160
CseI	GACGC	1 cut(s)	1033
Csp6I	GTAC	6 cut(s)	903, 1759, 1843, 1905, 2136, 2658
CspCI	CAANNNNNGTGG	4 cut(s)	786, 821, 1672, 1707
CviQI	GTAC	6 cut(s)	903, 1759, 1843, 1905, 2136, 2658
DraIII	CACNNNGTG	1 cut(s)	2116
EaeI	YGGCCR	3 cut(s)	174, 198, 2059
Eam1104I	CTCTTC	4 cut(s)	266, 387, 812, 893
EarI	CTCTTC	4 cut(s)	266, 387, 812, 893
EciI	GGCGGA	2 cut(s)	176, 1724
Ecl136II	GAGCTC	1 cut(s)	259
Eco130I	CCWWGG	4 cut(s)	21, 842, 863, 1103
Eco24I	GRGCYC	3 cut(s)	96, 261, 1274
Eco32I	GATATC	1 cut(s)	568
Eco47I	GGWCC	4 cut(s)	79, 1032, 1732, 2472
Eco53kI	GAGCTC	1 cut(s)	259
Eco57I	CTGAAG	6 cut(s)	1205, 1298, 1382, 1596, 2200, 2591
Eco88I	CYCGRG	1 cut(s)	1849
EcoICRI	GAGCTC	1 cut(s)	259
EcoNI	CCTNNNNNAGG	1 cut(s)	1643
EcoO109I	RGGNCCY	1 cut(s)	1032
EcoRI	GAATTC	1 cut(s)	1131
EcoRII	CCWGG	2 cut(s)	81, 1376
EcoRV	GATATC	1 cut(s)	568
EcoT14I	CCWWGG	4 cut(s)	21, 842, 863, 1103
EcoT38I	GRGCYC	3 cut(s)	96, 261, 1274
ErhI	CCWWGG	4 cut(s)	21, 842, 863, 1103
FalI	AAGNNNNNCTT	4 cut(s)	1380, 1412, 1656, 1688
FaqI	GGGAC	3 cut(s)	1018, 1894, 2393
FauI	CCCGC	1 cut(s)	161
FbaI	TGATCA	1 cut(s)	1018
FblI	GTMKAC	1 cut(s)	132
FokI	GGATG	6 cut(s)	4, 1466, 1621, 1640, 1889, 2036
FriOI	GRGCYC	3 cut(s)	96, 261, 1274
GlaI	GCGC	5 cut(s)	551, 792, 1182, 1539, 2045
GsaI	CCCAGC	1 cut(s)	1143
GsuI	CTGGAG	2 cut(s)	1215, 1328
HaeII	RGCGCY	1 cut(s)	794
HaeIII	GGCC	6 cut(s)	176, 200, 246, 453, 1688, 2061
HapII	CCGG	4 cut(s)	743, 2058, 2442, 2470
HgaI	GACGC	1 cut(s)	1033
HhaI	GCGC	5 cut(s)	552, 793, 1183, 1540, 2046
Hin6I	GCGC	5 cut(s)	550, 791, 1181, 1538, 2044
HinP1I	GCGC	5 cut(s)	550, 791, 1181, 1538, 2044
HincII	GTYRAC	4 cut(s)	133, 646, 1084, 1773
HindII	GTYRAC	4 cut(s)	133, 646, 1084, 1773
HindIII	AAGCTT	2 cut(s)	32, 1389
HinfI	GANTC	3 cut(s)	1373, 1769, 2260
HpaII	CCGG	4 cut(s)	743, 2058, 2442, 2470
HphI	GGTGA	5 cut(s)	272, 610, 2047, 2384, 2564
Hpy166II	GTNNAC	7 cut(s)	133, 347, 646, 1084, 1773, 1871, 2612
Hpy8I	GTNNAC	7 cut(s)	133, 347, 646, 1084, 1773, 1871, 2612
Hpy99I	CGWCG	3 cut(s)	134, 137, 1049
HpyAV	CCTTC	9 cut(s)	1032, 1159, 1180, 1211, 1276, 1465, 1618, 1742, 2566
HpyCH4III	ACNGT	9 cut(s)	122, 211, 460, 678, 781, 830, 986, 1817, 2326
HpyCH4IV	ACGT	5 cut(s)	129, 135, 838, 2330, 2666
HpySE526I	ACGT	5 cut(s)	129, 135, 838, 2330, 2666
HspAI	GCGC	5 cut(s)	550, 791, 1181, 1538, 2044
Kpn2I	TCCGGA	1 cut(s)	742
Ksp22I	TGATCA	1 cut(s)	1018
KspI	CCGCGG	1 cut(s)	160
LguI	GCTCTTC	2 cut(s)	266, 812
LmnI	GCTCC	5 cut(s)	1041, 1234, 1309, 1348, 2466
LweI	GCATC	4 cut(s)	1618, 2014, 2398, 2465
MaeII	ACGT	5 cut(s)	129, 135, 838, 2330, 2666
MaeIII	GTNAC	6 cut(s)	10, 986, 2108, 2165, 2326, 2552
MbiI	CCGCTC	1 cut(s)	1678
MfeI	CAATTG	2 cut(s)	495, 1257
MflI	RGATCY	3 cut(s)	1008, 1979, 2510
MhlI	GDGCHC	7 cut(s)	96, 261, 824, 1225, 1274, 1463, 2567
MlsI	TGGCCA	1 cut(s)	200
MluNI	TGGCCA	1 cut(s)	200
MlyI	GAGTC	2 cut(s)	1382, 1778
MmeI	TCCRAC	3 cut(s)	424, 1326, 1593
Mox20I	TGGCCA	1 cut(s)	200
MroI	TCCGGA	1 cut(s)	742
MscI	TGGCCA	1 cut(s)	200
MseI	TTAA	6 cut(s)	75, 527, 963, 977, 1173, 1595
MslI	CAYNNNNRTG	5 cut(s)	285, 382, 1815, 2202, 2253
Msp20I	TGGCCA	1 cut(s)	200
MspA1I	CMGCKG	2 cut(s)	159, 1605
MspI	CCGG	4 cut(s)	743, 2058, 2442, 2470
MspR9I	CCNGG	2 cut(s)	83, 1378
MunI	CAATTG	2 cut(s)	495, 1257
Mva1269I	GAATGC	1 cut(s)	1576
MvaI	CCWGG	2 cut(s)	83, 1378
MvnI	CGCG	3 cut(s)	159, 1181, 2347
NcoI	CCATGG	1 cut(s)	21
NlaIV	GGNNCC	3 cut(s)	406, 1034, 2468
NmuCI	GTSAC	3 cut(s)	986, 2108, 2552
NruI	TCGCGA	1 cut(s)	2347
NspI	RCATGY	3 cut(s)	633, 1791, 2362
NspV	TTCGAA	2 cut(s)	1385, 2003
OliI	CACNNNNGTG	1 cut(s)	285
PaqCI	CACCTGC	1 cut(s)	291
PciI	ACATGT	2 cut(s)	629, 2358
PciSI	GCTCTTC	2 cut(s)	266, 812
PcsI	WCGNNNNNNNCGW	1 cut(s)	844
PctI	GAATGC	1 cut(s)	1576
PfeI	GAWTC	1 cut(s)	2260
PflFI	GACNNNGTC	1 cut(s)	830
PflMI	CCANNNNNTGG	1 cut(s)	196
Ple19I	CGATCG	1 cut(s)	2134
PleI	GAGTC	2 cut(s)	1381, 1777
PpsI	GAGTC	2 cut(s)	1381, 1777
PpuMI	RGGWCCY	1 cut(s)	1032
PscI	ACATGT	2 cut(s)	629, 2358
Psp124BI	GAGCTC	1 cut(s)	261
Psp5II	RGGWCCY	1 cut(s)	1032
Psp6I	CCWGG	2 cut(s)	81, 1376
PspFI	CCCAGC	1 cut(s)	1139
PspGI	CCWGG	2 cut(s)	81, 1376
PspN4I	GGNNCC	3 cut(s)	406, 1034, 2468
PspPI	GGNCC	5 cut(s)	79, 452, 1032, 1732, 2472
PspPPI	RGGWCCY	1 cut(s)	1032
PsuI	RGATCY	3 cut(s)	1008, 1979, 2510
PsyI	GACNNNGTC	1 cut(s)	830
PvuI	CGATCG	1 cut(s)	2134
RruI	TCGCGA	1 cut(s)	2347
RsaI	GTAC	6 cut(s)	904, 1760, 1844, 1906, 2137, 2659
RsaNI	GTAC	6 cut(s)	903, 1759, 1843, 1905, 2136, 2658
RseI	CAYNNNNRTG	5 cut(s)	285, 382, 1815, 2202, 2253
SacI	GAGCTC	1 cut(s)	261
SacII	CCGCGG	1 cut(s)	160
SalI	GTCGAC	1 cut(s)	131
SapI	GCTCTTC	2 cut(s)	266, 812
SaqAI	TTAA	6 cut(s)	75, 527, 963, 977, 1173, 1595
Sau96I	GGNCC	5 cut(s)	79, 452, 1032, 1732, 2472
ScaI	AGTACT	2 cut(s)	904, 1760
SchI	GAGTC	2 cut(s)	1382, 1778
ScrFI	CCNGG	2 cut(s)	83, 1378
SduI	GDGCHC	7 cut(s)	96, 261, 824, 1225, 1274, 1463, 2567
SfaNI	GCATC	4 cut(s)	1618, 2014, 2398, 2465
SfcI	CTRYAG	2 cut(s)	2082, 2541
Sfr303I	CCGCGG	1 cut(s)	160
SfuI	TTCGAA	2 cut(s)	1385, 2003
SgrBI	CCGCGG	1 cut(s)	160
SgrDI	CGTCGACG	1 cut(s)	131
SinI	GGWCC	4 cut(s)	79, 1032, 1732, 2472
SmiMI	CAYNNNNRTG	5 cut(s)	285, 382, 1815, 2202, 2253
SmlI	CTYRAG	3 cut(s)	1673, 1682, 2101
SmoI	CTYRAG	3 cut(s)	1673, 1682, 2101
SsiI	CCGC	9 cut(s)	154, 157, 159, 187, 1201, 1421, 1603, 1678, 1735
SspI	AATATT	1 cut(s)	580
SstI	GAGCTC	1 cut(s)	261
StyD4I	CCNGG	2 cut(s)	81, 1376
StyI	CCWWGG	4 cut(s)	21, 842, 863, 1103
TaaI	ACNGT	9 cut(s)	122, 211, 460, 678, 781, 830, 986, 1817, 2326
TaiI	ACGT	5 cut(s)	132, 138, 841, 2333, 2669
TatI	WGTACW	5 cut(s)	902, 1758, 1842, 1904, 2657
TauI	GCSGC	4 cut(s)	159, 162, 1204, 1681
TfiI	GAWTC	1 cut(s)	2260
Tru1I	TTAA	6 cut(s)	75, 527, 963, 977, 1173, 1595
Tru9I	TTAA	6 cut(s)	75, 527, 963, 977, 1173, 1595
TscAI	CASTG	9 cut(s)	600, 615, 728, 784, 877, 991, 1696, 2204, 2377
TseFI	GTSAC	3 cut(s)	986, 2108, 2552
Tsp45I	GTSAC	3 cut(s)	986, 2108, 2552
TspDTI	ATGAA	8 cut(s)	6, 253, 651, 760, 1542, 1637, 1677, 1919
TspRI	CASTG	9 cut(s)	600, 615, 728, 784, 877, 991, 1696, 2204, 2377
Tth111I	GACNNNGTC	1 cut(s)	830
Van91I	CCANNNNNTGG	1 cut(s)	196
VpaK11BI	GGWCC	4 cut(s)	79, 1032, 1732, 2472
XagI	CCTNNNNNAGG	1 cut(s)	1643
XapI	RAATTY	2 cut(s)	1131, 1297
XbaI	TCTAGA	1 cut(s)	1501
XceI	RCATGY	3 cut(s)	633, 1791, 2362
XmaJI	CCTAGG	2 cut(s)	842, 863
XmiI	GTMKAC	1 cut(s)	132
ZrmI	AGTACT	2 cut(s)	904, 1760

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.