Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0009792

Physical Location & Seq

Genomic Coordinates Forward (+)

9596303 .. 9599183

Gene Structure 2881 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G010310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGTCGCAGGAGGAGGTAGCTAGGAAGGCGATTAAGCACGCGCTGAAGGCTCTAAGGAAGCGGCATTTGCTTGAAGAAGGTGCTCATGCTCCAGCGTATATCGCTCTCGCCAATCCAATTGCTCATCAGGGCTCTGAATGGAAGGAAAAGGCTGATAATTTGGAACTGGAGCTTCAGCAATGCTATAAAGCTCAATCTCGGCTGTCGGAGCAGCTTGTGGTGGAAGTAGCAGAGTCCAGGTCTTCAAAAGCTTTACTTCAGGAGAAAGAAGCAGCGATTGCGGATCAGCAGAATGAGCTGACACAAAAAAGGGATGAGTGCTCTCAGTTGAAGGCGGAGTTAGAAAAGAAGATTCAAGCTCTAGAGTTGGTTATGAGTGAGAACAATGAAATACGGGCGCAGCTAGAAGAAATGTCTGTTAGAGCTAAGAATGCTGAAGCTGAAAATAAGGTGTTAATTGACCGCTGGATGTTGGAAAAAATGAAGGATGCTGAACGCCTAAATGAGGCAAATGCACTATATGAAGAAATGCTTGAGCGGATCAAGGCCAGCGGTTTGCAAAAACTAGCCATGCAGCAAGTAGATGGTGTAGTCCGCCAAAGTGAAGAAGGTGCTGAGTACTTTGTGGAGTCAACCAATCCCACCACATGTAAGAACAGGATCAATGCCCATGACGGTGGCTGTGCTTCCATATTATTCGAGTACAACTCAGGCAAATTGATTTCTGGTGGACAGGATGGGACAATCAAAATGTGGGATACAAGTACAGGGGCTTTGAATAATACACTTCATGGTTGCATTGGTTCTGTTCTTGACCTTACCATTACCCATGATAATAGATCTATCATCGCAGCAAGCAGTTCGAACAAATTGTACGTATGGGATGCTAACTTGGGGAGAGTGCGCCATACTCTCACCGGCCACATGGATAAAGTTTGTGCTGTAGATGTGAGTAAGTTCTCAAGTCGTCACATTGTGAGTGCAGCCTATGATCGTACTATAAAAGTCTGGGATTTGCAAAAAGGTTACTGCACCAACACAATAATCTTCCCCAAACACTGCAATGCTCTTTGCTTCAGCATGGACGGGCAGACCATTTGCTCAGGTCATGTTGATGGGAATCTTCGGCTGTGGGATATTCAGAAAGGAAAACTACTCAACGAAGTGGCTGCACATTCAAATGCTGTTACGTCTATATCTTTGTCGCGAAGTGGCAACATGGTATTGACGAGTGGGAGGGACAATGTGCATCACCTTTTCGATATGAGAACCCTAGAAGTTTGTGGCACATTGAGGGCCACCGGAAACAGAGTAGCATCTAATTGGAGCCGGTCCTGTATCAGTCCAGATGACAATTACGTTGCTGCTGGATCTGCCGATGGTGCTGTGTATATCTGGTCTATAGCCAAAGGTGACATTGTGAGCACTCTGAAGGAACACACTAATTCTGTCCTCTGCTGCTCATGGAGCGGACATGGAAAACCTCTAGCTTCTGCGGACAAGAATGGAGTAATTTGTACTTGGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000421 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005776 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006897 GO:0006907 GO:0006909 GO:0006914 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016020 GO:0016043 GO:0016192 GO:0016236 GO:0016740 GO:0019538 GO:0019776 GO:0019941 GO:0022607 GO:0030163 GO:0030587 GO:0031090 GO:0031154 GO:0032502 GO:0032991 GO:0034274 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0051179 GO:0051234 GO:0051603 GO:0051703 GO:0051704 GO:0061919 GO:0070925 GO:0071704 GO:0071840 GO:0090382 GO:0090702 GO:0098588 GO:0098657 GO:0098805 GO:0099120 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905037 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04621 map04136 map04138 map04140 map04621

Pfam Domains

Protein Families

ATG16 WD40

Protein Analysis

509

Amino Acids

56.22

Weight (kDa)

6.4

Isoelectric Point (pI)

34.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG16	PF08614	44 - 168	2.7e-13	Autophagy protein 16 (ATG16)
Beta-prop_THOC3	PF25174	212 - 304	8.9e-12	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	213 - 349	7.7e-24	WDR5 beta-propeller domain
WDR55	PF24796	214 - 423	7.1e-18	WDR55
WD40_CDC20-Fz	PF24807	215 - 415	1e-21	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	216 - 341	1.5e-09	TEP-1 second beta-propeller
Beta-prop_SCAP	PF24017	216 - 297	4.1e-06	SCAP Beta-propeller
Beta-prop_WDR3_2nd	PF25172	217 - 295	1.3e-09	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	221 - 372	4.9e-16	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	221 - 349	3.1e-21	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	221 - 315	9.8e-08	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	223 - 506	7.5e-29	WDHD1 first WD40 domain
WD40_Prp19	PF24814	227 - 436	1.4e-29	Prp19 WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	236 - 339	2.9e-07	MABP1/WDR62 second WD40 domain
Beta-prop_THOC3	PF25174	272 - 507	1.6e-37	THOC3 beta-propeller domain
Beta-prop_ACSF4	PF13570	274 - 506	1.5e-08	Beta-alanine-activating enzyme, beta-propeller
WD40_Gbeta	PF25391	278 - 466	4.1e-19	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	280 - 356	9.2e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	282 - 416	3.5e-11	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	283 - 421	4e-12	Echinoderm microtubule-associated protein first beta-propeller
EIF3I	PF24805	291 - 366	1.1e-07	EIF3I
WD40	PF00400	300 - 338	1.7e-06	WD domain, G-beta repeat
NBCH_WD40	PF20426	300 - 470	1.1e-07	Neurobeachin beta propeller domain
Beta-prop_WDR3_2nd	PF25172	303 - 421	1e-12	WDR3 second beta-propeller domain
Beta-prop_SCAP	PF24017	317 - 476	4.3e-06	SCAP Beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	322 - 441	4.4e-10	WDR90/POC16, second beta-propeller
Beta-prop_EML_2	PF23414	322 - 434	2e-12	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	351 - 508	6.9e-14	WDR55
ANAPC4_WD40	PF12894	353 - 400	6.7e-06	Anaphase-promoting complex subunit 4 WD40 domain
WD40_CDC20-Fz	PF24807	353 - 493	6.8e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	354 - 439	1.2e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	354 - 440	3e-12	WDR3 first beta-propeller domain
EIF3I	PF24805	354 - 454	1.3e-11	EIF3I
Beta-prop_VPS8	PF23410	355 - 422	7.3e-06	Vps8 beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	356 - 423	3.8e-07	MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	356 - 428	1.4e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	358 - 429	2.3e-06	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	364 - 501	6.1e-09	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	372 - 499	6.1e-07	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	388 - 472	2e-06	TEP-1 second beta-propeller
WDR90_beta-prop_4th	PF23342	442 - 506	1.5e-06	WDR90, 4th beta-propeller
Beta-prop_WDR3_1st	PF25173	445 - 508	3.8e-10	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	446 - 508	3.6e-10	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	447 - 508	1.1e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	448 - 508	1.5e-06	Prp19 WD40 domain
Beta-prop_IFT140_1st	PF23383	449 - 508	6.5e-07	IFT140 first beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009792)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50230
fragaria_vesca	FvH4_5g06790 FvH4_5g06790
malus_domestica	MD06G1161200.v1.1 MD14G1166400.v1.1
prunus_persica	Prupe.5G160700_v2.0.a1 Prupe.5G160700_v2.0.a1
pyrus_communis	pycom06g14390 pycom14g13900
rosa_chinensis	RchiOBHm_Chr7g0191761 RchiOBHm_Chr7g0191771
rosa_laevigata	RLG00000004427
rosa_multiflora	Rmu_co8375695.1_g000001
rosa_roxburghii	Rroxscaffold_3G00263550
rosa_rugosa	Rorug06G0511200
rosa_samantha	Rh7AG121000 Rh7BG122700 Rh7CG125600 Rh7DG124300
rosa_wichuraiana	Rw7G010300 Rw7G010310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	538, 1470
AccII	CGCG	2 cut(s)	41, 1207
AciI	CCGC	9 cut(s)	61, 281, 335, 463, 538, 552, 595, 1470, 1496
AclWI	GGATC	4 cut(s)	291, 548, 668, 1378
AcoI	YGGCCR	1 cut(s)	919
AcuI	CTGAAG	6 cut(s)	65, 158, 242, 456, 1060, 1451
AdeI	CACNNNGTG	1 cut(s)	976
AfaI	GTAC	6 cut(s)	620, 704, 766, 875, 997, 1519
AfiI	CCNNNNNNNGG	1 cut(s)	505
AflIII	ACRYGT	1 cut(s)	647
AgsI	TTSAA	6 cut(s)	74, 246, 331, 356, 778, 1179
AjiI	CACGTC	1 cut(s)	1527
AjnI	CCWGG	1 cut(s)	236
Alw21I	GWGCWC	3 cut(s)	85, 323, 1427
AlwI	GGATC	4 cut(s)	291, 548, 668, 1378
AoxI	GGCC	3 cut(s)	546, 919, 1296
ApeKI	GCWGC	9 cut(s)	211, 272, 400, 574, 851, 983, 1169, 1364, 1458
AspLEI	GCGC	3 cut(s)	43, 400, 906
AspS9I	GGNCC	2 cut(s)	1296, 1332
AsuHPI	GGTGA	3 cut(s)	907, 1244, 1424
AsuII	TTCGAA	1 cut(s)	863
AvaII	GGWCC	1 cut(s)	1332
BanII	GRGCYC	1 cut(s)	134
BbsI	GAAGAC	1 cut(s)	234
Bbv12I	GWGCWC	3 cut(s)	85, 323, 1427
BbvI	GCAGC	9 cut(s)	223, 284, 412, 586, 863, 995, 1156, 1351, 1445
BccI	CCATC	4 cut(s)	578, 731, 1109, 1373
BcgI	CGANNNNNNTGC	4 cut(s)	843, 877, 1151, 1185
BciT130I	CCWGG	1 cut(s)	238
BciVI	GTATCC	1 cut(s)	751
BfaI	CTAG	6 cut(s)	21, 362, 404, 566, 1274, 1487
BfmI	CTRYAG	2 cut(s)	942, 1401
BfuI	GTATCC	1 cut(s)	751
BglII	AGATCT	1 cut(s)	839
BmcAI	AGTACT	1 cut(s)	620
Bme1390I	CCNGG	1 cut(s)	238
Bme18I	GGWCC	1 cut(s)	1332
BmgBI	CACGTC	1 cut(s)	1527
BmgT120I	GGNCC	2 cut(s)	1296, 1332
BmiI	GGNNCC	1 cut(s)	1328
BmrFI	CCNGG	1 cut(s)	238
BmsI	GCATC	4 cut(s)	478, 874, 1258, 1325
BpiI	GAAGAC	1 cut(s)	234
BplI	GAGNNNNNCTC	2 cut(s)	692, 724
BpmI	CTGGAG	2 cut(s)	75, 188
Bpu10I	CCTNAGC	1 cut(s)	1102
Bpu14I	TTCGAA	1 cut(s)	863
BpuEI	CTTGAG	2 cut(s)	554, 946
BsaAI	YACGTR	1 cut(s)	877
BsaBI	GATNNNNATC	1 cut(s)	1119
BsaWI	WCCGGW	1 cut(s)	1301
Bsc4I	CCNNNNNNNGG	1 cut(s)	505
Bse118I	RCCGGY	2 cut(s)	917, 1329
Bse1I	ACTGG	1 cut(s)	171
Bse3DI	GCAATG	2 cut(s)	185, 1069
Bse8I	GATNNNNATC	1 cut(s)	1119
BseBI	CCWGG	1 cut(s)	238
BseGI	GGATG	5 cut(s)	319, 474, 493, 742, 889
BseJI	GATNNNNATC	1 cut(s)	1119
BseLI	CCNNNNNNNGG	1 cut(s)	505
BseMI	GCAATG	2 cut(s)	185, 1069
BseMII	CTCAG	4 cut(s)	338, 606, 723, 1116
BseNI	ACTGG	1 cut(s)	171
BseRI	GAGGAG	1 cut(s)	26
BseXI	GCAGC	9 cut(s)	223, 284, 412, 586, 863, 995, 1156, 1351, 1445
BsgI	GTGCAG	3 cut(s)	1002, 1015, 1155
Bsh1236I	CGCG	2 cut(s)	41, 1207
BshFI	GGCC	3 cut(s)	548, 921, 1298
BsiHKAI	GWGCWC	3 cut(s)	85, 323, 1427
BsiSI	CCGG	3 cut(s)	918, 1302, 1330
BslFI	GGGAC	2 cut(s)	754, 1253
BslI	CCNNNNNNNGG	1 cut(s)	505
BsmFI	GGGAC	2 cut(s)	754, 1253
BsmI	GAATGC	1 cut(s)	436
BsnI	GGCC	3 cut(s)	548, 921, 1298
Bsp119I	TTCGAA	1 cut(s)	863
Bsp1286I	GDGCHC	4 cut(s)	85, 134, 323, 1427
Bsp143I	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
Bsp68I	TCGCGA	1 cut(s)	1207
BspACI	CCGC	9 cut(s)	61, 281, 335, 463, 538, 552, 595, 1470, 1496
BspANI	GGCC	3 cut(s)	548, 921, 1298
BspCNI	CTCAG	4 cut(s)	337, 607, 722, 1115
BspFNI	CGCG	2 cut(s)	41, 1207
BspLI	GGNNCC	1 cut(s)	1328
BspPI	GGATC	4 cut(s)	291, 548, 668, 1378
BspT104I	TTCGAA	1 cut(s)	863
BsrBI	CCGCTC	2 cut(s)	538, 1470
BsrDI	GCAATG	2 cut(s)	185, 1069
BsrFI	RCCGGY	2 cut(s)	917, 1329
BsrI	ACTGG	1 cut(s)	171
BssAI	RCCGGY	2 cut(s)	917, 1329
BssMI	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
Bst2UI	CCWGG	1 cut(s)	238
Bst4CI	ACNGT	1 cut(s)	677
BstAPI	GCANNNNNTGC	1 cut(s)	278
BstBAI	YACGTR	1 cut(s)	877
BstBI	TTCGAA	1 cut(s)	863
BstC8I	GCNNGC	3 cut(s)	39, 550, 856
BstDEI	CTNAG	6 cut(s)	53, 324, 426, 615, 709, 1102
BstENI	CCTNNNNNAGG	1 cut(s)	503
BstF5I	GGATG	5 cut(s)	319, 474, 493, 742, 889
BstFNI	CGCG	2 cut(s)	41, 1207
BstHHI	GCGC	3 cut(s)	43, 400, 906
BstKTI	GATC	6 cut(s)	286, 543, 663, 842, 994, 1373
BstMBI	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
BstNI	CCWGG	1 cut(s)	238
BstNSI	RCATGY	1 cut(s)	651
BstSCI	CCNGG	1 cut(s)	236
BstSFI	CTRYAG	2 cut(s)	942, 1401
BstSNI	TACGTA	1 cut(s)	877
BstUI	CGCG	2 cut(s)	41, 1207
BstV1I	GCAGC	9 cut(s)	223, 284, 412, 586, 863, 995, 1156, 1351, 1445
BstV2I	GAAGAC	1 cut(s)	234
BstX2I	RGATCY	2 cut(s)	839, 1370
BstXI	CCANNNNNNTGG	1 cut(s)	677
BstYI	RGATCY	2 cut(s)	839, 1370
BsuI	GTATCC	1 cut(s)	751
BsuRI	GGCC	3 cut(s)	548, 921, 1298
BtgZI	GCGATG	1 cut(s)	832
BtrI	CACGTC	1 cut(s)	1527
BtsCI	GGATG	5 cut(s)	319, 474, 493, 742, 889
BtsI	GCAGTG	1 cut(s)	1057
BtsIMutI	CAGTG	1 cut(s)	1057
BtuMI	TCGCGA	1 cut(s)	1207
Cac8I	GCNNGC	3 cut(s)	39, 550, 856
CfoI	GCGC	3 cut(s)	43, 400, 906
Cfr10I	RCCGGY	2 cut(s)	917, 1329
Cfr13I	GGNCC	2 cut(s)	1296, 1332
Csp6I	GTAC	6 cut(s)	619, 703, 765, 874, 996, 1518
CviQI	GTAC	6 cut(s)	619, 703, 765, 874, 996, 1518
DdeI	CTNAG	6 cut(s)	53, 324, 426, 615, 709, 1102
DpnI	GATC	6 cut(s)	285, 542, 662, 841, 993, 1372
DpnII	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
DraIII	CACNNNGTG	1 cut(s)	976
EaeI	YGGCCR	1 cut(s)	919
EciI	GGCGGA	2 cut(s)	350, 584
Eco105I	TACGTA	1 cut(s)	877
Eco24I	GRGCYC	1 cut(s)	134
Eco47I	GGWCC	1 cut(s)	1332
Eco57I	CTGAAG	6 cut(s)	65, 158, 242, 456, 1060, 1451
EcoNI	CCTNNNNNAGG	1 cut(s)	503
EcoRII	CCWGG	1 cut(s)	236
EcoT38I	GRGCYC	1 cut(s)	134
FalI	AAGNNNNNCTT	4 cut(s)	240, 272, 516, 548
FaqI	GGGAC	2 cut(s)	754, 1253
FokI	GGATG	5 cut(s)	326, 481, 500, 749, 896
FriOI	GRGCYC	1 cut(s)	134
FspBI	CTAG	6 cut(s)	21, 362, 404, 566, 1274, 1487
GlaI	GCGC	3 cut(s)	42, 399, 905
GsuI	CTGGAG	2 cut(s)	75, 188
HaeIII	GGCC	3 cut(s)	548, 921, 1298
HapII	CCGG	3 cut(s)	918, 1302, 1330
HhaI	GCGC	3 cut(s)	43, 400, 906
Hin6I	GCGC	3 cut(s)	41, 398, 904
HinP1I	GCGC	3 cut(s)	41, 398, 904
HincII	GTYRAC	1 cut(s)	633
HindII	GTYRAC	1 cut(s)	633
HindIII	AAGCTT	1 cut(s)	249
HinfI	GANTC	4 cut(s)	233, 352, 629, 1120
HpaII	CCGG	3 cut(s)	918, 1302, 1330
HphI	GGTGA	3 cut(s)	907, 1244, 1424
Hpy166II	GTNNAC	2 cut(s)	633, 731
Hpy188I	TCNGA	4 cut(s)	136, 208, 1143, 1431
Hpy188III	TCNNGA	5 cut(s)	260, 362, 812, 1206, 1346
Hpy8I	GTNNAC	2 cut(s)	633, 731
HpyAV	CCTTC	8 cut(s)	19, 40, 71, 136, 325, 478, 602, 1426
HpyCH4III	ACNGT	1 cut(s)	677
HpyCH4IV	ACGT	4 cut(s)	876, 1190, 1359, 1526
HpyF3I	CTNAG	6 cut(s)	53, 324, 426, 615, 709, 1102
HpySE526I	ACGT	4 cut(s)	876, 1190, 1359, 1526
HspAI	GCGC	3 cut(s)	41, 398, 904
Kzo9I	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
LmnI	GCTCC	5 cut(s)	94, 169, 208, 1326, 1467
Lsp1109I	GCAGC	9 cut(s)	223, 284, 412, 586, 863, 995, 1156, 1351, 1445
LweI	GCATC	4 cut(s)	478, 874, 1258, 1325
MaeI	CTAG	6 cut(s)	21, 362, 404, 566, 1274, 1487
MaeII	ACGT	4 cut(s)	876, 1190, 1359, 1526
MaeIII	GTNAC	4 cut(s)	968, 1025, 1186, 1412
MalI	GATC	6 cut(s)	285, 542, 662, 841, 993, 1372
MbiI	CCGCTC	2 cut(s)	538, 1470
MboI	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
MboII	GAAGA	8 cut(s)	86, 234, 361, 419, 536, 617, 1039, 1115
MfeI	CAATTG	1 cut(s)	117
MflI	RGATCY	2 cut(s)	839, 1370
MhlI	GDGCHC	4 cut(s)	85, 134, 323, 1427
MluCI	AATT	9 cut(s)	117, 157, 456, 716, 869, 1321, 1354, 1444, 1512
MlyI	GAGTC	2 cut(s)	242, 638
MmeI	TCCRAC	2 cut(s)	186, 453
MnlI	CCTC	7 cut(s)	4, 7, 499, 1230, 1287, 1463, 1494
MseI	TTAA	2 cut(s)	33, 455
MslI	CAYNNNNRTG	4 cut(s)	675, 1062, 1113, 1179
MspA1I	CMGCKG	2 cut(s)	465, 552
MspI	CCGG	3 cut(s)	918, 1302, 1330
MspR9I	CCNGG	1 cut(s)	238
MunI	CAATTG	1 cut(s)	117
Mva1269I	GAATGC	1 cut(s)	436
MvaI	CCWGG	1 cut(s)	238
MvnI	CGCG	2 cut(s)	41, 1207
NdeII	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
NlaIV	GGNNCC	1 cut(s)	1328
NmeAIII	GCCGAG	1 cut(s)	178
NmuCI	GTSAC	2 cut(s)	968, 1412
NruI	TCGCGA	1 cut(s)	1207
NspI	RCATGY	1 cut(s)	651
NspV	TTCGAA	1 cut(s)	863
PciI	ACATGT	1 cut(s)	647
PctI	GAATGC	1 cut(s)	436
PfeI	GAWTC	2 cut(s)	352, 1120
PleI	GAGTC	2 cut(s)	241, 637
PpsI	GAGTC	2 cut(s)	241, 637
Ppu21I	YACGTR	1 cut(s)	877
PscI	ACATGT	1 cut(s)	647
Psp6I	CCWGG	1 cut(s)	236
PspGI	CCWGG	1 cut(s)	236
PspN4I	GGNNCC	1 cut(s)	1328
PspPI	GGNCC	2 cut(s)	1296, 1332
PsrI	GAACNNNNNNTAC	2 cut(s)	857, 889
PsuI	RGATCY	2 cut(s)	839, 1370
RruI	TCGCGA	1 cut(s)	1207
RsaI	GTAC	6 cut(s)	620, 704, 766, 875, 997, 1519
RsaNI	GTAC	6 cut(s)	619, 703, 765, 874, 996, 1518
RseI	CAYNNNNRTG	4 cut(s)	675, 1062, 1113, 1179
SaqAI	TTAA	2 cut(s)	33, 455
Sau3AI	GATC	6 cut(s)	283, 540, 660, 839, 991, 1370
Sau96I	GGNCC	2 cut(s)	1296, 1332
ScaI	AGTACT	1 cut(s)	620
SchI	GAGTC	2 cut(s)	242, 638
ScrFI	CCNGG	1 cut(s)	238
SduI	GDGCHC	4 cut(s)	85, 134, 323, 1427
SfaNI	GCATC	4 cut(s)	478, 874, 1258, 1325
SfcI	CTRYAG	2 cut(s)	942, 1401
SfuI	TTCGAA	1 cut(s)	863
SinI	GGWCC	1 cut(s)	1332
SmiMI	CAYNNNNRTG	4 cut(s)	675, 1062, 1113, 1179
SmlI	CTYRAG	2 cut(s)	533, 961
SmoI	CTYRAG	2 cut(s)	533, 961
SnaBI	TACGTA	1 cut(s)	877
Sse9I	AATT	9 cut(s)	117, 157, 456, 716, 869, 1321, 1354, 1444, 1512
SsiI	CCGC	9 cut(s)	61, 281, 335, 463, 538, 552, 595, 1470, 1496
SspMI	CTAG	6 cut(s)	21, 362, 404, 566, 1274, 1487
StyD4I	CCNGG	1 cut(s)	236
TaaI	ACNGT	1 cut(s)	677
TaiI	ACGT	4 cut(s)	879, 1193, 1362, 1529
TaqI	TCGA	3 cut(s)	699, 863, 1260
TasI	AATT	9 cut(s)	117, 157, 456, 716, 869, 1321, 1354, 1444, 1512
TatI	WGTACW	4 cut(s)	618, 702, 764, 1517
TauI	GCSGC	1 cut(s)	64
TfiI	GAWTC	2 cut(s)	352, 1120
Tru1I	TTAA	2 cut(s)	33, 455
Tru9I	TTAA	2 cut(s)	33, 455
TscAI	CASTG	1 cut(s)	1064
TseFI	GTSAC	2 cut(s)	968, 1412
TseI	GCWGC	9 cut(s)	211, 272, 400, 574, 851, 983, 1169, 1364, 1458
Tsp45I	GTSAC	2 cut(s)	968, 1412
TspDTI	ATGAA	4 cut(s)	402, 497, 537, 779
TspRI	CASTG	1 cut(s)	1064
VpaK11BI	GGWCC	1 cut(s)	1332
XagI	CCTNNNNNAGG	1 cut(s)	503
XbaI	TCTAGA	1 cut(s)	361
XceI	RCATGY	1 cut(s)	651
XspI	CTAG	6 cut(s)	21, 362, 404, 566, 1274, 1487
ZrmI	AGTACT	1 cut(s)	620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.