Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010652

Physical Location & Seq

Genomic Coordinates Forward (+)

32397585 .. 32400106

Gene Structure 2522 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1186100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGGGGGTGGACTACTACAACATACTGAAAGTTAATCGGAACGCGAGCGAGGACGACCTGAAGAAGGCCTACAAGCGGCTCGCCATGATTTGGCACCCGGACAAGAACCCCGCCGGGAAGCGGCCGGAGGCGGAGGCCAAGTTCAAGCAAATCTCCGAGGCCTACGACGTCCTCAGCGACCCCGCCAAGCGCCAGATCTACGACCTCTACGGCGAGGAGGCTCTGAAATCGGGGCAACTTCCGCCTCCCTCTTCTTCCTCCTCCGCCCGCTCCTCCGCCGCTTCGGGCCACCACTACTACGCCAACCACAACAACCAGCGCCACGCACACCCGAATACGTCGTCGTTCACGTTCAGTCCCAGGGACGCCGAGGACATTTACGCCGAGATTTTTGGATCCGACGGCGACGGAGGCGGGATTGGGGGCGGAGGGAGGTATAGAGATGGGTTCTTTAGGACGTCCAATGGTGGTGGGCCAGAGTTTGCGGGACCCAGTGGGGCTGCGCCTGCGAGGAAGGCCAATGCGGTTGAGAATTTGTTGCCCTGCAGCTTGGAGGAGCTCTACAAGGGTGTCAAGAAGAAGATGAAGATTTCCAGAAACGTCTACGATGCCGTTGGTGCTGGTAAGGTTCGGACTGTGGAGGAGATTTTGACCATTGAGATCAGACCAGGTTGGAAGAAGGGCACAAAGATCACCTTCCCTGAGAAGGGTAACCAAGAGCAAGGTGTGATTCCAGCGGATCTAATTTTTATTGTAGACGAGAAACCACACGCACTTTACAAGAGGGACAGTAATGACCTGGTGGTCAACCAGGAGATAACACTGCTGGAGGCACTCACTGGCAAGACTCTTGACCTTACCACCCTAGATGGACGGAATCTCATGATCCCGTTGACTGATATCATCAAACCCGGAGCTGAAATGGTGGTACCAAATGAGGGAATGCCGATCTCAAAAGAACCTGGAAAAAAGGGAAATTTGAGAATCAAGTTTGATGTCAAGTATCCTTCAAGGCTTACCACAGAACAGAAATCTGATCTGAAAAGAGTTCTGGGAGGAGTTTCATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001671 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0008047 GO:0008150 GO:0016020 GO:0030234 GO:0031974 GO:0031981 GO:0032781 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043462 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0050790 GO:0051087 GO:0051336 GO:0051345 GO:0060589 GO:0060590 GO:0065007 GO:0065009 GO:0070013 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ DnaJ_C

Protein Analysis

357

Amino Acids

39.02

Weight (kDa)

8.92

Isoelectric Point (pI)

49.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	4 - 68	4.7e-26	DnaJ domain
DnaJ_C	PF01556	180 - 336	6e-40	DnaJ C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010652)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47940
fragaria_vesca	FvH4_5g15710
malus_domestica	MD06G1186100.v1.1 MD07G1162200.v1.1 MD07G1162300.v1.1 MD14G1192300.v1.1
prunus_persica	Prupe.5G184900_v2.0.a1
pyrus_communis	pycom06g16550 pycom14g16000
rosa_chinensis	RchiOBHm_Chr7g0180401
rosa_laevigata	RLG00000005270
rosa_multiflora	Rmu_sc0018872.1_g000002 Rmu_sc0042636.1_g000001
rosa_roxburghii	Rroxscaffold_3G00272920
rosa_rugosa	Rorug06G0432800
rosa_samantha	Rh7AG033400 Rh7BG033300 Rh7CG035300 Rh7DG033800
rosa_wichuraiana	Rw7G002790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	803
AatII	GACGTC	2 cut(s)	171, 461
Acc65I	GGTACC	1 cut(s)	928
AccB1I	GGYRCC	2 cut(s)	93, 928
AccB7I	CCANNNNNTGG	1 cut(s)	90
AccBSI	CCGCTC	1 cut(s)	270
AccI	GTMKAC	2 cut(s)	603, 756
AccII	CGCG	1 cut(s)	44
AclWI	GGATC	4 cut(s)	390, 403, 747, 880
AcoI	YGGCCR	1 cut(s)	122
AcsI	RAATTY	2 cut(s)	532, 976
AcuI	CTGAAG	1 cut(s)	80
AcyI	GRCGYC	3 cut(s)	168, 366, 458
AfaI	GTAC	1 cut(s)	930
AfiI	CCNNNNNNNGG	7 cut(s)	64, 75, 90, 120, 706, 707, 938
AgsI	TTSAA	2 cut(s)	145, 1011
AjnI	CCWGG	5 cut(s)	359, 667, 798, 810, 961
AloI	GAACNNNNNNTCC	2 cut(s)	125, 157
AluBI	AGCT	3 cut(s)	549, 559, 917
AluI	AGCT	3 cut(s)	549, 559, 917
Alw21I	GWGCWC	1 cut(s)	561
AlwI	GGATC	4 cut(s)	390, 403, 747, 880
AoxI	GGCC	7 cut(s)	66, 122, 135, 159, 286, 473, 516
ApeKI	GCWGC	2 cut(s)	500, 546
ApoI	RAATTY	2 cut(s)	532, 976
ArsI	GACNNNNNNTTYG	2 cut(s)	326, 358
Asp718I	GGTACC	1 cut(s)	928
AspLEI	GCGC	3 cut(s)	192, 321, 505
AspS9I	GGNCC	3 cut(s)	286, 473, 488
AsuC2I	CCSGG	3 cut(s)	98, 115, 912
AsuHPI	GGTGA	1 cut(s)	685
AvaII	GGWCC	1 cut(s)	488
BaeGI	GKGCMC	1 cut(s)	686
BamHI	GGATCC	1 cut(s)	395
BanI	GGYRCC	2 cut(s)	93, 928
BanII	GRGCYC	1 cut(s)	561
BarI	GAAGNNNNNNTAC	2 cut(s)	53, 85
Bbv12I	GWGCWC	1 cut(s)	561
BbvCI	CCTCAGC	1 cut(s)	173
BbvI	GCAGC	2 cut(s)	487, 558
BccI	CCATC	2 cut(s)	437, 863
BceAI	ACGGC	3 cut(s)	226, 418, 596
BciT130I	CCWGG	5 cut(s)	361, 669, 800, 812, 963
BciVI	GTATCC	1 cut(s)	1014
BcnI	CCSGG	3 cut(s)	98, 115, 912
BfaI	CTAG	1 cut(s)	866
BfmI	CTRYAG	1 cut(s)	544
BfoI	RGCGCY	2 cut(s)	193, 322
BfuI	GTATCC	1 cut(s)	1014
BglII	AGATCT	1 cut(s)	195
BisI	GCNGC	5 cut(s)	77, 122, 279, 501, 547
BlsI	GCNGC	5 cut(s)	78, 123, 280, 502, 548
Bme1390I	CCNGG	8 cut(s)	98, 115, 361, 669, 800, 812, 912, 963
Bme18I	GGWCC	1 cut(s)	488
BmgT120I	GGNCC	3 cut(s)	286, 473, 488
BmiI	GGNNCC	5 cut(s)	95, 397, 489, 490, 930
BmrFI	CCNGG	8 cut(s)	98, 115, 361, 669, 800, 812, 912, 963
BmrI	ACTGGG	1 cut(s)	486
BmsI	GCATC	1 cut(s)	598
BmuI	ACTGGG	1 cut(s)	486
BpmI	CTGGAG	1 cut(s)	848
Bpu10I	CCTNAGC	1 cut(s)	173
BpuMI	CCSGG	3 cut(s)	98, 115, 912
BsaHI	GRCGYC	3 cut(s)	168, 366, 458
BsaJI	CCNNGG	4 cut(s)	156, 359, 360, 369
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	7 cut(s)	64, 75, 90, 120, 706, 707, 938
Bse1I	ACTGG	2 cut(s)	492, 844
BseBI	CCWGG	5 cut(s)	361, 669, 800, 812, 963
BseDI	CCNNGG	4 cut(s)	156, 359, 360, 369
BseLI	CCNNNNNNNGG	7 cut(s)	64, 75, 90, 120, 706, 707, 938
BseMII	CTCAG	2 cut(s)	187, 693
BseNI	ACTGG	2 cut(s)	492, 844
BseRI	GAGGAG	6 cut(s)	230, 250, 262, 569, 656, 1071
BseSI	GKGCMC	1 cut(s)	686
BseX3I	CGGCCG	1 cut(s)	122
BseXI	GCAGC	2 cut(s)	487, 558
Bsh1236I	CGCG	1 cut(s)	44
Bsh1285I	CGRYCG	1 cut(s)	125
BshFI	GGCC	7 cut(s)	68, 124, 137, 161, 288, 475, 518
BshNI	GGYRCC	2 cut(s)	93, 928
BsiEI	CGRYCG	1 cut(s)	125
BsiHKAI	GWGCWC	1 cut(s)	561
BsiSI	CCGG	4 cut(s)	98, 114, 125, 912
BslFI	GGGAC	4 cut(s)	342, 377, 501, 800
BslI	CCNNNNNNNGG	7 cut(s)	64, 75, 90, 120, 706, 707, 938
BsmFI	GGGAC	4 cut(s)	342, 377, 501, 800
BsmI	GAATGC	1 cut(s)	948
BsnI	GGCC	7 cut(s)	68, 124, 137, 161, 288, 475, 518
Bsp1286I	GDGCHC	2 cut(s)	561, 686
Bsp143I	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
BspANI	GGCC	7 cut(s)	68, 124, 137, 161, 288, 475, 518
BspCNI	CTCAG	2 cut(s)	186, 694
BspFNI	CGCG	1 cut(s)	44
BspHI	TCATGA	1 cut(s)	882
BspLI	GGNNCC	5 cut(s)	95, 397, 489, 490, 930
BspMAI	CTGCAG	1 cut(s)	548
BspPI	GGATC	4 cut(s)	390, 403, 747, 880
BspT107I	GGYRCC	2 cut(s)	93, 928
BsrBI	CCGCTC	1 cut(s)	270
BsrI	ACTGG	2 cut(s)	492, 844
BssECI	CCNNGG	4 cut(s)	156, 359, 360, 369
BssMI	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
BssNI	GRCGYC	3 cut(s)	168, 366, 458
Bst2UI	CCWGG	5 cut(s)	361, 669, 800, 812, 963
Bst4CI	ACNGT	2 cut(s)	637, 791
Bst6I	CTCTTC	1 cut(s)	256
BstACI	GRCGYC	3 cut(s)	168, 366, 458
BstC8I	GCNNGC	4 cut(s)	46, 81, 268, 507
BstDEI	CTNAG	2 cut(s)	173, 702
BstEII	GGTNACC	1 cut(s)	710
BstENI	CCTNNNNNAGG	1 cut(s)	62
BstFNI	CGCG	1 cut(s)	44
BstH2I	RGCGCY	2 cut(s)	193, 322
BstHHI	GCGC	3 cut(s)	192, 321, 505
BstKTI	GATC	8 cut(s)	198, 398, 663, 693, 742, 888, 951, 1039
BstMBI	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
BstMCI	CGRYCG	1 cut(s)	125
BstMWI	GCNNNNNNNGC	5 cut(s)	241, 411, 506, 515, 617
BstNI	CCWGG	5 cut(s)	361, 669, 800, 812, 963
BstPI	GGTNACC	1 cut(s)	710
BstSCI	CCNGG	8 cut(s)	96, 113, 359, 667, 798, 810, 910, 961
BstSFI	CTRYAG	1 cut(s)	544
BstSLI	GKGCMC	1 cut(s)	686
BstUI	CGCG	1 cut(s)	44
BstV1I	GCAGC	2 cut(s)	487, 558
BstX2I	RGATCY	3 cut(s)	195, 395, 739
BstYI	RGATCY	3 cut(s)	195, 395, 739
BstZI	CGGCCG	1 cut(s)	122
BsuI	GTATCC	1 cut(s)	1014
BsuRI	GGCC	7 cut(s)	68, 124, 137, 161, 288, 475, 518
BtsI	GCAGTG	1 cut(s)	821
BtsIMutI	CAGTG	3 cut(s)	499, 821, 837
Cac8I	GCNNGC	4 cut(s)	46, 81, 268, 507
CciI	TCATGA	1 cut(s)	882
CfoI	GCGC	3 cut(s)	192, 321, 505
Cfr13I	GGNCC	3 cut(s)	286, 473, 488
CseI	GACGC	1 cut(s)	374
CsiI	ACCWGGT	2 cut(s)	667, 798
Csp6I	GTAC	1 cut(s)	929
CspCI	CAANNNNNGTGG	2 cut(s)	451, 486
CviAII	CATG	2 cut(s)	85, 883
CviQI	GTAC	1 cut(s)	929
DdeI	CTNAG	2 cut(s)	173, 702
DpnI	GATC	8 cut(s)	197, 397, 662, 692, 741, 887, 950, 1038
DpnII	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
DrdI	GACNNNNNNGTC	1 cut(s)	803
DseDI	GACNNNNNNGTC	1 cut(s)	803
EaeI	YGGCCR	1 cut(s)	122
EagI	CGGCCG	1 cut(s)	122
Eam1104I	CTCTTC	1 cut(s)	256
EarI	CTCTTC	1 cut(s)	256
EciI	GGCGGA	5 cut(s)	146, 231, 253, 265, 441
Ecl136II	GAGCTC	1 cut(s)	559
EclXI	CGGCCG	1 cut(s)	122
Eco147I	AGGCCT	2 cut(s)	68, 161
Eco24I	GRGCYC	1 cut(s)	561
Eco32I	GATATC	1 cut(s)	901
Eco47I	GGWCC	1 cut(s)	488
Eco52I	CGGCCG	1 cut(s)	122
Eco53kI	GAGCTC	1 cut(s)	559
Eco57I	CTGAAG	1 cut(s)	80
Eco91I	GGTNACC	1 cut(s)	710
EcoICRI	GAGCTC	1 cut(s)	559
EcoNI	CCTNNNNNAGG	1 cut(s)	62
EcoO109I	RGGNCCY	1 cut(s)	488
EcoO65I	GGTNACC	1 cut(s)	710
EcoRII	CCWGG	5 cut(s)	359, 667, 798, 810, 961
EcoRV	GATATC	1 cut(s)	901
EcoT38I	GRGCYC	1 cut(s)	561
FaeI	CATG	2 cut(s)	88, 886
FaiI	YATR	5 cut(s)	23, 86, 438, 884, 1069
FalI	AAGNNNNNCTT	2 cut(s)	680, 712
FaqI	GGGAC	4 cut(s)	342, 377, 501, 800
FatI	CATG	2 cut(s)	84, 882
FauI	CCCGC	5 cut(s)	118, 190, 275, 407, 478
FblI	GTMKAC	2 cut(s)	603, 756
Fnu4HI	GCNGC	5 cut(s)	77, 122, 279, 501, 547
FriOI	GRGCYC	1 cut(s)	561
Fsp4HI	GCNGC	5 cut(s)	77, 122, 279, 501, 547
FspBI	CTAG	1 cut(s)	866
GlaI	GCGC	3 cut(s)	191, 320, 504
GluI	GCNGC	5 cut(s)	77, 122, 279, 501, 547
GsuI	CTGGAG	1 cut(s)	848
HaeII	RGCGCY	2 cut(s)	193, 322
HaeIII	GGCC	7 cut(s)	68, 124, 137, 161, 288, 475, 518
HapII	CCGG	4 cut(s)	98, 114, 125, 912
HgaI	GACGC	1 cut(s)	374
HhaI	GCGC	3 cut(s)	192, 321, 505
Hin1I	GRCGYC	3 cut(s)	168, 366, 458
Hin1II	CATG	2 cut(s)	88, 886
Hin6I	GCGC	3 cut(s)	190, 319, 503
HinP1I	GCGC	3 cut(s)	190, 319, 503
HincII	GTYRAC	2 cut(s)	808, 894
HindII	GTYRAC	2 cut(s)	808, 894
HinfI	GANTC	4 cut(s)	730, 847, 877, 984
HpaII	CCGG	4 cut(s)	98, 114, 125, 912
HphI	GGTGA	1 cut(s)	685
Hpy166II	GTNNAC	6 cut(s)	10, 348, 604, 757, 808, 894
Hpy188I	TCNGA	8 cut(s)	39, 157, 225, 400, 633, 665, 1036, 1041
Hpy188III	TCNNGA	4 cut(s)	574, 594, 851, 883
Hpy8I	GTNNAC	6 cut(s)	10, 348, 604, 757, 808, 894
Hpy99I	CGWCG	5 cut(s)	170, 343, 346, 404, 410
HpyAV	CCTTC	6 cut(s)	58, 508, 673, 700, 706, 1017
HpyCH4III	ACNGT	2 cut(s)	637, 791
HpyCH4IV	ACGT	5 cut(s)	168, 338, 350, 458, 600
HpyCH4V	TGCA	1 cut(s)	546
HpyF10VI	GCNNNNNNNGC	5 cut(s)	241, 411, 506, 515, 617
HpyF3I	CTNAG	2 cut(s)	173, 702
HpySE526I	ACGT	5 cut(s)	168, 338, 350, 458, 600
Hsp92I	GRCGYC	3 cut(s)	168, 366, 458
Hsp92II	CATG	2 cut(s)	88, 886
HspAI	GCGC	3 cut(s)	190, 319, 503
KflI	GGGWCCC	1 cut(s)	488
KpnI	GGTACC	1 cut(s)	932
Kzo9I	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
LmnI	GCTCC	3 cut(s)	275, 556, 914
Lsp1109I	GCAGC	2 cut(s)	487, 558
LweI	GCATC	1 cut(s)	598
MabI	ACCWGGT	2 cut(s)	667, 798
MaeI	CTAG	1 cut(s)	866
MaeII	ACGT	5 cut(s)	168, 338, 350, 458, 600
MaeIII	GTNAC	1 cut(s)	710
MalI	GATC	8 cut(s)	197, 397, 662, 692, 741, 887, 950, 1038
MbiI	CCGCTC	1 cut(s)	270
MboI	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
MboII	GAAGA	7 cut(s)	73, 243, 246, 589, 592, 598, 688
MflI	RGATCY	3 cut(s)	195, 395, 739
MhlI	GDGCHC	2 cut(s)	561, 686
MluCI	AATT	3 cut(s)	532, 744, 976
MlyI	GAGTC	1 cut(s)	841
MmeI	TCCRAC	2 cut(s)	423, 653
MseI	TTAA	1 cut(s)	33
MspA1I	CMGCKG	1 cut(s)	737
MspI	CCGG	4 cut(s)	98, 114, 125, 912
MspR9I	CCNGG	8 cut(s)	98, 115, 361, 669, 800, 812, 912, 963
Mva1269I	GAATGC	1 cut(s)	948
MvaI	CCWGG	5 cut(s)	361, 669, 800, 812, 963
MvnI	CGCG	1 cut(s)	44
MwoI	GCNNNNNNNGC	5 cut(s)	241, 411, 506, 515, 617
NciI	CCSGG	3 cut(s)	98, 115, 912
NdeII	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
NlaIII	CATG	2 cut(s)	88, 886
NlaIV	GGNNCC	5 cut(s)	95, 397, 489, 490, 930
NmeAIII	GCCGAG	2 cut(s)	394, 409
PagI	TCATGA	1 cut(s)	882
PasI	CCCWGGG	1 cut(s)	360
PceI	AGGCCT	2 cut(s)	68, 161
PcsI	WCGNNNNNNNCGW	2 cut(s)	174, 347
PctI	GAATGC	1 cut(s)	948
PfeI	GAWTC	3 cut(s)	730, 877, 984
PflMI	CCANNNNNTGG	1 cut(s)	90
PkrI	GCNGC	5 cut(s)	78, 123, 280, 502, 548
PleI	GAGTC	1 cut(s)	841
PpsI	GAGTC	1 cut(s)	841
PpuMI	RGGWCCY	1 cut(s)	488
Psp124BI	GAGCTC	1 cut(s)	561
Psp5II	RGGWCCY	1 cut(s)	488
Psp6I	CCWGG	5 cut(s)	359, 667, 798, 810, 961
PspEI	GGTNACC	1 cut(s)	710
PspGI	CCWGG	5 cut(s)	359, 667, 798, 810, 961
PspN4I	GGNNCC	5 cut(s)	95, 397, 489, 490, 930
PspPI	GGNCC	3 cut(s)	286, 473, 488
PspPPI	RGGWCCY	1 cut(s)	488
PstI	CTGCAG	1 cut(s)	548
PsuI	RGATCY	3 cut(s)	195, 395, 739
RsaI	GTAC	1 cut(s)	930
RsaNI	GTAC	1 cut(s)	929
SacI	GAGCTC	1 cut(s)	561
SaqAI	TTAA	1 cut(s)	33
SatI	GCNGC	5 cut(s)	77, 122, 279, 501, 547
Sau3AI	GATC	8 cut(s)	195, 395, 660, 690, 739, 885, 948, 1036
Sau96I	GGNCC	3 cut(s)	286, 473, 488
SchI	GAGTC	1 cut(s)	841
ScrFI	CCNGG	8 cut(s)	98, 115, 361, 669, 800, 812, 912, 963
SduI	GDGCHC	2 cut(s)	561, 686
SexAI	ACCWGGT	2 cut(s)	667, 798
SfaNI	GCATC	1 cut(s)	598
SfcI	CTRYAG	1 cut(s)	544
SinI	GGWCC	1 cut(s)	488
Sse9I	AATT	3 cut(s)	532, 744, 976
SseBI	AGGCCT	2 cut(s)	68, 161
SspMI	CTAG	1 cut(s)	866
SstI	GAGCTC	1 cut(s)	561
StuI	AGGCCT	2 cut(s)	68, 161
StyD4I	CCNGG	8 cut(s)	96, 113, 359, 667, 798, 810, 910, 961
TaaI	ACNGT	2 cut(s)	637, 791
TaiI	ACGT	5 cut(s)	171, 341, 353, 461, 603
TasI	AATT	3 cut(s)	532, 744, 976
TauI	GCSGC	3 cut(s)	79, 124, 281
TfiI	GAWTC	3 cut(s)	730, 877, 984
Tru1I	TTAA	1 cut(s)	33
Tru9I	TTAA	1 cut(s)	33
TscAI	CASTG	3 cut(s)	499, 828, 844
TseI	GCWGC	2 cut(s)	500, 546
TspDTI	ATGAA	2 cut(s)	599, 1053
TspGWI	ACGGA	2 cut(s)	423, 889
TspRI	CASTG	3 cut(s)	499, 828, 844
Van91I	CCANNNNNTGG	1 cut(s)	90
VpaK11BI	GGWCC	1 cut(s)	488
XagI	CCTNNNNNAGG	1 cut(s)	62
XapI	RAATTY	2 cut(s)	532, 976
XmiI	GTMKAC	2 cut(s)	603, 756
XspI	CTAG	1 cut(s)	866
ZraI	GACGTC	2 cut(s)	169, 459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.