MD06G1227200.v1.1

Zinc finger protein VAR3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006886
Physical Location & Seq
Forward (+)
35790385 .. 35799918
9534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1227200.v1.1.491

Sequence Viewer

Length: 813 bp
ATGACTTGGTTTTATCAGGTTTTAGGTGGTGGTTGTAATCTCCGAGGTTCCTGTGATAGAGCTTATGTGATTCTTAAGGAATCTGAAGCAGCAGCACGTACAGTGGATATAGTGCGAATACTATTGTTTTATGCGCTGGATCCCATTGTTTTTTATGAGGGAGAGAAACCGCCTGGTAGAGAGATTCTAGAAACATCTGCAAGAAAGCTGCTTTCTGAGTTGCTTCAACTCAGCGAAAGGGCTGTTGACCCATCACTTCCAAAGCCTGCTGCCAAAGCTATTGAGAAGAAGAAAAATTATCCAAGTTTCATTGGTGATGAACTATCTCAAGATGTTGAAATGAAGAGAGGAGATTGGATGTGTCCCAAGTGCAATTTTATGAACTTTGCTAAAAATTTAAGATGCCTACAATGTAAAGAAGATGGCCCAAGAAAAGCTGACACGGGTGATCTCGAAATCGAAATGAAAAAGGGAGACTGGATCTGCACTGAGTGCAGTTTCATGAACTTCTCTAGAAATATACGCTGCCTAAAGTGCAAAGCTGAAGGGCCAAAGAGCGTTGGTGTACATGCAGTTGAAATGAAGAAAGGAGACTGGACCTGCCCAAAGTGTGCATTCATGAATTTTGCAAGCAATAGGAAGTGTTTGCGTTGTCAAGATCCACGGCCCAAGAAAAATCCTGCAGACTGGGACTGCCCCTCATGTGATTTCATGAATTATGGTAGAAACGCAGTTTGCCTGAAGTGCAACTGTAAACGCCCTCAAGAAGAAACTACTGAGTACGAGGAGCAGATGTGGAGGCGTCCACGCTAG

Protein Analysis

271

Amino Acids

31.04

Weight (kDa)

8.63

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_RanBP PF00641 116 - 139 3.1e-08 Zn-finger in Ran binding protein and others
Zn_ribbon_RanBP PF00641 156 - 184 1e-08 Zn-finger in Ran binding protein and others
Zn_ribbon_RanBP PF00641 195 - 223 4.6e-10 Zn-finger in Ran binding protein and others
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 608
AciI CCGC 1 cut(s) 170
AclWI GGATC 4 cut(s) 134, 147, 488, 653
AcsI RAATTY 2 cut(s) 394, 622
AcuI CTGAAG 3 cut(s) 105, 564, 761
AcyI GRCGYC 1 cut(s) 802
AdeI CACNNNGTG 1 cut(s) 492
AfaI GTAC 3 cut(s) 100, 567, 782
AflII CTTAAG 1 cut(s) 74
AgsI TTSAA 3 cut(s) 227, 338, 578
AjnI CCWGG 1 cut(s) 172
AluBI AGCT 5 cut(s) 62, 208, 278, 437, 542
AluI AGCT 5 cut(s) 62, 208, 278, 437, 542
Alw26I GTCTC 2 cut(s) 468, 585
AlwI GGATC 4 cut(s) 134, 147, 488, 653
AoxI GGCC 3 cut(s) 424, 548, 665
ApeKI GCWGC 5 cut(s) 89, 92, 208, 269, 525
ApoI RAATTY 2 cut(s) 394, 622
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 4 cut(s) 425, 548, 597, 666
AsuHPI GGTGA 2 cut(s) 326, 458
AvaII GGWCC 1 cut(s) 597
BamHI GGATCC 1 cut(s) 139
BbvI GCAGC 5 cut(s) 101, 104, 195, 256, 512
BccI CCATC 2 cut(s) 259, 416
BceAI ACGGC 1 cut(s) 680
BciT130I CCWGG 1 cut(s) 174
BcoDI GTCTC 2 cut(s) 468, 585
BfaI CTAG 3 cut(s) 188, 513, 811
BfmI CTRYAG 1 cut(s) 681
BfrI CTTAAG 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 608
BisI GCNGC 5 cut(s) 90, 93, 209, 270, 526
BlsI GCNGC 5 cut(s) 91, 94, 210, 271, 527
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 597
BmgT120I GGNCC 4 cut(s) 425, 548, 597, 666
BmiI GGNNCC 2 cut(s) 49, 141
BmrFI CCNGG 1 cut(s) 174
BmrI ACTGGG 1 cut(s) 697
BmsI GCATC 1 cut(s) 392
BmuI ACTGGG 1 cut(s) 697
BpuEI CTTGAG 2 cut(s) 312, 747
BsaAI YACGTR 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 802
BsaJI CCNNGG 2 cut(s) 43, 662
BsaXI ACNNNNNCTCC 2 cut(s) 779, 809
Bse1I ACTGG 3 cut(s) 482, 599, 692
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 43, 662
BseGI GGATG 1 cut(s) 363
BseMII CTCAG 4 cut(s) 207, 244, 480, 768
BseNI ACTGG 3 cut(s) 482, 599, 692
BseRI GAGGAG 2 cut(s) 363, 800
BseXI GCAGC 5 cut(s) 101, 104, 195, 256, 512
BsgI GTGCAG 2 cut(s) 469, 514
BshFI GGCC 3 cut(s) 426, 550, 667
BslFI GGGAC 2 cut(s) 348, 704
BsmAI GTCTC 2 cut(s) 468, 585
BsmFI GGGAC 2 cut(s) 348, 704
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 3 cut(s) 426, 550, 667
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 4 cut(s) 139, 448, 480, 658
BspACI CCGC 1 cut(s) 170
BspANI GGCC 3 cut(s) 426, 550, 667
BspCNI CTCAG 4 cut(s) 208, 243, 481, 769
BspHI TCATGA 3 cut(s) 501, 618, 711
BspLI GGNNCC 2 cut(s) 49, 141
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 1 cut(s) 608
BspPI GGATC 4 cut(s) 134, 147, 488, 653
BspTI CTTAAG 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 3 cut(s) 482, 599, 692
BssECI CCNNGG 2 cut(s) 43, 662
BssMI GATC 4 cut(s) 139, 448, 480, 658
BssNI GRCGYC 1 cut(s) 802
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 103, 752
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 802
BstAFI CTTAAG 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 492
BstAUI TGTACA 1 cut(s) 565
BstBAI YACGTR 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 267, 631
BstDEI CTNAG 4 cut(s) 216, 230, 489, 777
BstDSI CCRYGG 1 cut(s) 662
BstF5I GGATG 1 cut(s) 363
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 4 cut(s) 142, 451, 483, 661
BstMAI GTCTC 2 cut(s) 468, 585
BstMBI GATC 4 cut(s) 139, 448, 480, 658
BstMWI GCNNNNNNNGC 4 cut(s) 275, 492, 534, 744
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 681
BstV1I GCAGC 5 cut(s) 101, 104, 195, 256, 512
BstX2I RGATCY 3 cut(s) 139, 480, 658
BstYI RGATCY 3 cut(s) 139, 480, 658
BsuRI GGCC 3 cut(s) 426, 550, 667
BtgI CCRYGG 1 cut(s) 662
BtsCI GGATG 1 cut(s) 363
BtsIMutI CAGTG 2 cut(s) 108, 486
BveI ACCTGC 1 cut(s) 608
Cac8I GCNNGC 2 cut(s) 267, 631
CciI TCATGA 3 cut(s) 501, 618, 711
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 4 cut(s) 425, 548, 597, 666
CseI GACGC 1 cut(s) 791
Csp6I GTAC 3 cut(s) 99, 566, 781
CviAII CATG 5 cut(s) 502, 569, 619, 702, 712
CviQI GTAC 3 cut(s) 99, 566, 781
DdeI CTNAG 4 cut(s) 216, 230, 489, 777
DpnI GATC 4 cut(s) 141, 450, 482, 660
DpnII GATC 4 cut(s) 139, 448, 480, 658
DraIII CACNNNGTG 1 cut(s) 492
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 597
Eco57I CTGAAG 3 cut(s) 105, 564, 761
EcoRII CCWGG 1 cut(s) 172
FaeI CATG 5 cut(s) 505, 572, 622, 705, 715
FaqI GGGAC 2 cut(s) 348, 704
FatI CATG 5 cut(s) 501, 568, 618, 701, 711
Fnu4HI GCNGC 5 cut(s) 90, 93, 209, 270, 526
FokI GGATG 1 cut(s) 370
Fsp4HI GCNGC 5 cut(s) 90, 93, 209, 270, 526
FspBI CTAG 3 cut(s) 188, 513, 811
GlaI GCGC 1 cut(s) 135
GluI GCNGC 5 cut(s) 90, 93, 209, 270, 526
HaeIII GGCC 3 cut(s) 426, 550, 667
HgaI GACGC 1 cut(s) 791
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 802
Hin1II CATG 5 cut(s) 505, 572, 622, 705, 715
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 70, 80, 184
HphI GGTGA 2 cut(s) 326, 458
Hpy166II GTNNAC 4 cut(s) 247, 566, 755, 806
Hpy188I TCNGA 3 cut(s) 44, 85, 217
Hpy188III TCNNGA 9 cut(s) 188, 329, 452, 502, 513, 619, 656, 712, 764
Hpy8I GTNNAC 4 cut(s) 247, 566, 755, 806
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 2 cut(s) 103, 752
HpyCH4IV ACGT 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 492, 534, 744
HpyF3I CTNAG 4 cut(s) 216, 230, 489, 777
HpySE526I ACGT 1 cut(s) 97
Hsp92I GRCGYC 1 cut(s) 802
Hsp92II CATG 5 cut(s) 505, 572, 622, 705, 715
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 4 cut(s) 139, 448, 480, 658
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 5 cut(s) 101, 104, 195, 256, 512
LweI GCATC 1 cut(s) 392
MaeI CTAG 3 cut(s) 188, 513, 811
MaeII ACGT 1 cut(s) 97
MalI GATC 4 cut(s) 141, 450, 482, 660
MboI GATC 4 cut(s) 139, 448, 480, 658
MboII GAAGA 6 cut(s) 298, 301, 355, 431, 595, 779
MflI RGATCY 3 cut(s) 139, 480, 658
MluCI AATT 5 cut(s) 295, 373, 394, 622, 715
MnlI CCTC 7 cut(s) 38, 151, 341, 709, 771, 778, 792
MseI TTAA 2 cut(s) 75, 398
MspCI CTTAAG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 614
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 275, 492, 534, 744
NdeII GATC 4 cut(s) 139, 448, 480, 658
NlaIII CATG 5 cut(s) 505, 572, 622, 705, 715
NlaIV GGNNCC 2 cut(s) 49, 141
NspI RCATGY 1 cut(s) 572
PagI TCATGA 3 cut(s) 501, 618, 711
PctI GAATGC 1 cut(s) 614
PfeI GAWTC 3 cut(s) 70, 80, 184
PkrI GCNGC 5 cut(s) 91, 94, 210, 271, 527
Ppu21I YACGTR 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 2 cut(s) 49, 141
PspPI GGNCC 4 cut(s) 425, 548, 597, 666
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 3 cut(s) 139, 480, 658
RsaI GTAC 3 cut(s) 100, 567, 782
RsaNI GTAC 3 cut(s) 99, 566, 781
SaqAI TTAA 2 cut(s) 75, 398
SatI GCNGC 5 cut(s) 90, 93, 209, 270, 526
Sau3AI GATC 4 cut(s) 139, 448, 480, 658
Sau96I GGNCC 4 cut(s) 425, 548, 597, 666
ScrFI CCNGG 1 cut(s) 174
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 1 cut(s) 597
SmlI CTYRAG 3 cut(s) 74, 327, 762
SmoI CTYRAG 3 cut(s) 74, 327, 762
Sse9I AATT 5 cut(s) 295, 373, 394, 622, 715
SsiI CCGC 1 cut(s) 170
SspMI CTAG 3 cut(s) 188, 513, 811
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 2 cut(s) 103, 752
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 453, 459
TasI AATT 5 cut(s) 295, 373, 394, 622, 715
TatI WGTACW 1 cut(s) 565
TfiI GAWTC 3 cut(s) 70, 80, 184
Tru1I TTAA 2 cut(s) 75, 398
Tru9I TTAA 2 cut(s) 75, 398
TscAI CASTG 2 cut(s) 108, 493
TseI GCWGC 5 cut(s) 89, 92, 208, 269, 525
TspRI CASTG 2 cut(s) 108, 493
Vha464I CTTAAG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 597
XapI RAATTY 2 cut(s) 394, 622
XbaI TCTAGA 2 cut(s) 187, 512
XceI RCATGY 1 cut(s) 572
XspI CTAG 3 cut(s) 188, 513, 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.