MD06G1231300.v1.1

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015046
Physical Location & Seq
Reverse (-)
36090474 .. 36093488
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1231300.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGTGGATGATGGGTTATAATGACGGTGGCCATGGCGATTTCAACATGCCGGATTCTTTCAATGGACGGAAGCTCCGCACTCTCATTCCGAGGTCGCTTCCCTCTGTAAACAACACTTCTTCGACCATCACAACTCCTCCTTGTCTAAACCGATCAGCAATTTATGGCTCTCACGATTTCCTTACGCAGTATCACCAACTCGCCGCTATGGCTGAACAAAACAAGAGATCGGAATTTCTCAACACGCCACAGGTTGTGGTGAGCTCAAGATGGAACCCTACGCCGGAGCAGCTGAGGGCTCTTGAAGAATTATACAGACGAGGGACTCGAACGCCGTCGGCAGAGCAGATTCAGCACATCACGGCACAGCTTCGAAAATATGGAAAGATAGAAGGGAAGAATGTCTTCTATTGGTTCCAAAACCACAAGGCCAGAGAAAGGCAGAAACGTCGCCGTCAAATGGAATCATCTGCCCTCTCTGCAGAACACGACCAGCAACTGCAAATATCAATGACGTCTACAGATAATCAAAAGAAAGAAGAATTATTAGGGGCAAGTAGGACAGGTTTTGAAGTCGCAGAGACCAAAAACTGGGCACCCTCTACAAATTGCAGTACTGCTACATATGTTGAGGAATCTGTTTCAATACAAAGAACAGCAAAAGCAGCGGCAGCAGAGTGCAGGACGGATGGATGGATCCAATTCGACGAAGAAGAATTGGAGCAAAGAAGAAACTTTGTAGAAAGGAATGCCACGTGGCAGATGATGCAGTTGTCTTGTCCTTCTCACCCTCACCCTCACCCTCCCACCCACCTCATAAACACCCCTCCTACTTGTGATAGTACGACAGCCAGATCTAGGGCAGCAGCTACAGTAAGAAGAACGGACCAAAGGCTCTTTAAGTCTCATGATCTCAGCATCTTCATAGCACCCTACACATCAGAAACTGGTTTCAACACTGAGCAAGATCATGGATGTGAGGAATCTCAAACCCTTCAACTCTTTCCTCTTAGAAGTGGTCATGTCAAGGCCAGTGCCAATGCCAACGAGTTAAGCGTTAACGATAAGGATCATACGGAGCTATCCGCTAGCTTCAGTGCTCATCCTTACCCCTTTTTTGAGTTCCTTCCATTAAAGAACTAA

Protein Analysis

381

Amino Acids

43.31

Weight (kDa)

7.26

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 89 - 148 2e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015046)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18010
fragaria_vesca FvH4_5g17270
malus_domestica MD06G1231300.v1.1 MD14G1235800.v1.1
prunus_persica Prupe.5G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178511
rosa_laevigata RLG00000005441
rosa_multiflora Rmu_sc0002421.1_g000001 Rmu_sc0016060.1_g000002
rosa_roxburghii Rroxscaffold_3G00274850
rosa_rugosa Rorug06G0417700
rosa_samantha Rh7AG016200 Rh7BG015700 Rh7CG016400 Rh7DG016300
rosa_wichuraiana Rw7G001280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 18
AatII GACGTC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 591
AccI GTMKAC 1 cut(s) 518
AciI CCGC 4 cut(s) 76, 204, 668, 1086
AclWI GGATC 3 cut(s) 691, 704, 1077
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 1 cut(s) 233
AcuI CTGAAG 1 cut(s) 1078
AcvI CACGTG 1 cut(s) 756
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 2 cut(s) 616, 844
AfiI CCNNNNNNNGG 5 cut(s) 283, 438, 460, 591, 858
AgsI TTSAA 7 cut(s) 43, 61, 305, 572, 645, 955, 998
AluBI AGCT 7 cut(s) 73, 264, 292, 370, 869, 1081, 1092
AluI AGCT 7 cut(s) 73, 264, 292, 370, 869, 1081, 1092
Alw21I GWGCWC 2 cut(s) 266, 1102
Alw26I GTCTC 2 cut(s) 575, 909
AlwI GGATC 3 cut(s) 691, 704, 1077
AlwNI CAGNNNCTG 2 cut(s) 499, 947
AoxI GGCC 3 cut(s) 28, 429, 1029
ApeKI GCWGC 5 cut(s) 289, 665, 671, 863, 866
ApoI RAATTY 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 886
AsuHPI GGTGA 5 cut(s) 185, 271, 779, 785, 791
AsuII TTCGAA 1 cut(s) 373
AsuNHI GCTAGC 1 cut(s) 1088
AvaII GGWCC 1 cut(s) 886
BaeGI GKGCMC 1 cut(s) 598
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 696
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 266, 301
BbrPI CACGTG 1 cut(s) 756
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 2 cut(s) 266, 1102
BbvCI CCTCAGC 1 cut(s) 293
BbvI GCAGC 5 cut(s) 301, 677, 683, 875, 878
BccI CCATC 5 cut(s) 4, 134, 264, 683, 687
BceAI ACGGC 3 cut(s) 319, 378, 438
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BcoDI GTCTC 2 cut(s) 575, 909
BfaI CTAG 2 cut(s) 858, 1089
BfmI CTRYAG 3 cut(s) 480, 519, 870
BglI GCCNNNNNGGC 1 cut(s) 209
BglII AGATCT 1 cut(s) 854
BisI GCNGC 7 cut(s) 204, 290, 666, 669, 672, 864, 867
BlsI GCNGC 7 cut(s) 205, 291, 667, 670, 673, 865, 868
BmcAI AGTACT 1 cut(s) 616
Bme18I GGWCC 1 cut(s) 886
BmgT120I GGNCC 1 cut(s) 886
BmiI GGNNCC 4 cut(s) 275, 416, 597, 698
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 2 cut(s) 756, 927
BmtI GCTAGC 1 cut(s) 1092
BmuI ACTGGG 1 cut(s) 601
BpiI GAAGAC 1 cut(s) 397
Bpu10I CCTNAGC 1 cut(s) 293
Bpu14I TTCGAA 1 cut(s) 373
BpuEI CTTGAG 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 756
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 31, 89
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 121, 151
Bsc4I CCNNNNNNNGG 5 cut(s) 283, 438, 460, 591, 858
Bse1I ACTGG 3 cut(s) 596, 952, 1032
Bse8I GATNNNNATC 1 cut(s) 1068
BseDI CCNNGG 2 cut(s) 31, 89
BseGI GGATG 5 cut(s) 12, 694, 698, 980, 1102
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 5 cut(s) 283, 438, 460, 591, 858
BseMII CTCAG 3 cut(s) 284, 928, 951
BseNI ACTGG 3 cut(s) 596, 952, 1032
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 5 cut(s) 301, 677, 683, 875, 878
BsgI GTGCAG 1 cut(s) 700
BshFI GGCC 3 cut(s) 30, 431, 1031
BshNI GGYRCC 1 cut(s) 595
BsiHKAI GWGCWC 2 cut(s) 266, 1102
BsiSI CCGG 2 cut(s) 50, 284
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 5 cut(s) 283, 438, 460, 591, 858
BsmAI GTCTC 2 cut(s) 575, 909
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 3 cut(s) 30, 431, 1031
Bso31I GGTCTC 1 cut(s) 575
Bsp119I TTCGAA 1 cut(s) 373
Bsp1286I GDGCHC 4 cut(s) 266, 301, 598, 1102
Bsp143I GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 4 cut(s) 76, 204, 668, 1086
BspANI GGCC 3 cut(s) 30, 431, 1031
BspCNI CTCAG 3 cut(s) 285, 927, 952
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 4 cut(s) 275, 416, 597, 698
BspMAI CTGCAG 1 cut(s) 484
BspOI GCTAGC 1 cut(s) 1092
BspPI GGATC 3 cut(s) 691, 704, 1077
BspT104I TTCGAA 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 575
BsrI ACTGG 3 cut(s) 596, 952, 1032
BssECI CCNNGG 2 cut(s) 31, 89
BssMI GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
BssNI GRCGYC 1 cut(s) 515
BssT1I CCWWGG 1 cut(s) 31
Bst4CI ACNGT 2 cut(s) 26, 874
BstACI GRCGYC 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 756
BstBI TTCGAA 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 1090
BstDEI CTNAG 4 cut(s) 293, 914, 960, 1010
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 5 cut(s) 12, 694, 698, 980, 1102
BstKTI GATC 7 cut(s) 155, 230, 699, 857, 913, 970, 1072
BstMAI GTCTC 2 cut(s) 575, 909
BstMBI GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
BstMWI GCNNNNNNNGC 7 cut(s) 209, 289, 352, 479, 665, 671, 766
BstNSI RCATGY 1 cut(s) 49
BstSFI CTRYAG 3 cut(s) 480, 519, 870
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 5 cut(s) 301, 677, 683, 875, 878
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 2 cut(s) 696, 854
BstYI RGATCY 2 cut(s) 696, 854
BsuRI GGCC 3 cut(s) 30, 431, 1031
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 5 cut(s) 12, 694, 698, 980, 1102
BtsIMutI CAGTG 3 cut(s) 957, 1039, 1102
Cac8I GCNNGC 1 cut(s) 1090
CaiI CAGNNNCTG 2 cut(s) 499, 947
CciI TCATGA 1 cut(s) 907
Cfr13I GGNCC 1 cut(s) 886
Csp6I GTAC 2 cut(s) 615, 843
CviAII CATG 5 cut(s) 32, 46, 908, 971, 1022
CviQI GTAC 2 cut(s) 615, 843
DdeI CTNAG 4 cut(s) 293, 914, 960, 1010
DpnI GATC 7 cut(s) 154, 229, 698, 856, 912, 969, 1071
DpnII GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
EaeI YGGCCR 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 266, 301
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 1078
Eco72I CACGTG 1 cut(s) 756
EcoICRI GAGCTC 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 266, 301
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 5 cut(s) 35, 49, 911, 974, 1025
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 1 cut(s) 337
FatI CATG 5 cut(s) 31, 45, 907, 970, 1021
FauNDI CATATG 1 cut(s) 625
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 7 cut(s) 204, 290, 666, 669, 672, 864, 867
FokI GGATG 5 cut(s) 19, 701, 705, 987, 1089
FriOI GRGCYC 2 cut(s) 266, 301
Fsp4HI GCNGC 7 cut(s) 204, 290, 666, 669, 672, 864, 867
FspBI CTAG 2 cut(s) 858, 1089
GluI GCNGC 7 cut(s) 204, 290, 666, 669, 672, 864, 867
HaeIII GGCC 3 cut(s) 30, 431, 1031
HapII CCGG 2 cut(s) 50, 284
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 5 cut(s) 35, 49, 911, 974, 1025
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 6 cut(s) 53, 325, 349, 464, 635, 983
HpaI GTTAAC 1 cut(s) 1060
HpaII CCGG 2 cut(s) 50, 284
HphI GGTGA 5 cut(s) 185, 271, 779, 785, 791
Hpy166II GTNNAC 3 cut(s) 109, 519, 1060
Hpy188I TCNGA 3 cut(s) 90, 232, 943
Hpy188III TCNNGA 4 cut(s) 173, 267, 302, 908
Hpy8I GTNNAC 3 cut(s) 109, 519, 1060
Hpy99I CGWCG 3 cut(s) 340, 453, 710
HpyAV CCTTC 4 cut(s) 386, 792, 1004, 1136
HpyCH4III ACNGT 2 cut(s) 26, 874
HpyCH4IV ACGT 3 cut(s) 448, 515, 755
HpyCH4V TGCA 5 cut(s) 482, 502, 612, 681, 769
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 289, 352, 479, 665, 671, 766
HpyF3I CTNAG 4 cut(s) 293, 914, 960, 1010
HpySE526I ACGT 3 cut(s) 448, 515, 755
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 5 cut(s) 35, 49, 911, 974, 1025
KspAI GTTAAC 1 cut(s) 1060
Kzo9I GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
LmnI GCTCC 4 cut(s) 78, 286, 721, 1078
Lsp1109I GCAGC 5 cut(s) 301, 677, 683, 875, 878
LweI GCATC 2 cut(s) 756, 927
MaeI CTAG 2 cut(s) 858, 1089
MaeII ACGT 3 cut(s) 448, 515, 755
MalI GATC 7 cut(s) 154, 229, 698, 856, 912, 969, 1071
MboI GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
MflI RGATCY 2 cut(s) 696, 854
MhlI GDGCHC 4 cut(s) 266, 301, 598, 1102
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 7 cut(s) 159, 233, 308, 542, 607, 701, 716
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 1 cut(s) 319
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 404
MscI TGGCCA 1 cut(s) 30
MseI TTAA 4 cut(s) 900, 1052, 1059, 1133
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 2 cut(s) 292, 668
MspI CCGG 2 cut(s) 50, 284
Mva1269I GAATGC 1 cut(s) 754
MwoI GCNNNNNNNGC 7 cut(s) 209, 289, 352, 479, 665, 671, 766
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 625
NdeII GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
NheI GCTAGC 1 cut(s) 1088
NlaIII CATG 5 cut(s) 35, 49, 911, 974, 1025
NlaIV GGNNCC 4 cut(s) 275, 416, 597, 698
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 373
PagI TCATGA 1 cut(s) 907
PcsI WCGNNNNNNNCGW 2 cut(s) 325, 1053
PctI GAATGC 1 cut(s) 754
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 5 cut(s) 53, 349, 464, 635, 983
PflMI CCANNNNNTGG 1 cut(s) 591
PkrI GCNGC 7 cut(s) 205, 291, 667, 670, 673, 865, 868
PleI GAGTC 1 cut(s) 319
PmaCI CACGTG 1 cut(s) 756
PmlI CACGTG 1 cut(s) 756
PpsI GAGTC 1 cut(s) 319
Ppu21I YACGTR 1 cut(s) 756
PsiI TTATAA 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 266
PspCI CACGTG 1 cut(s) 756
PspN4I GGNNCC 4 cut(s) 275, 416, 597, 698
PspPI GGNCC 1 cut(s) 886
PstI CTGCAG 1 cut(s) 484
PstNI CAGNNNCTG 2 cut(s) 499, 947
PsuI RGATCY 2 cut(s) 696, 854
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 2 cut(s) 616, 844
RsaNI GTAC 2 cut(s) 615, 843
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 266
SaqAI TTAA 4 cut(s) 900, 1052, 1059, 1133
SatI GCNGC 7 cut(s) 204, 290, 666, 669, 672, 864, 867
Sau3AI GATC 7 cut(s) 152, 227, 696, 854, 910, 967, 1069
Sau96I GGNCC 1 cut(s) 886
ScaI AGTACT 1 cut(s) 616
SchI GAGTC 1 cut(s) 319
SduI GDGCHC 4 cut(s) 266, 301, 598, 1102
SfaNI GCATC 2 cut(s) 756, 927
SfcI CTRYAG 3 cut(s) 480, 519, 870
SfuI TTCGAA 1 cut(s) 373
SinI GGWCC 1 cut(s) 886
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
Sse9I AATT 7 cut(s) 159, 233, 308, 542, 607, 701, 716
SsiI CCGC 4 cut(s) 76, 204, 668, 1086
SspMI CTAG 2 cut(s) 858, 1089
SstI GAGCTC 1 cut(s) 266
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 26, 874
TaiI ACGT 3 cut(s) 451, 518, 758
TaqI TCGA 4 cut(s) 122, 328, 373, 705
TasI AATT 7 cut(s) 159, 233, 308, 542, 607, 701, 716
TatI WGTACW 1 cut(s) 614
TauI GCSGC 2 cut(s) 206, 671
TfiI GAWTC 5 cut(s) 53, 349, 464, 635, 983
Tru1I TTAA 4 cut(s) 900, 1052, 1059, 1133
Tru9I TTAA 4 cut(s) 900, 1052, 1059, 1133
TscAI CASTG 3 cut(s) 964, 1039, 1102
TseI GCWGC 5 cut(s) 289, 665, 671, 863, 866
TspDTI ATGAA 1 cut(s) 913
TspGWI ACGGA 4 cut(s) 82, 701, 899, 1091
TspRI CASTG 3 cut(s) 964, 1039, 1102
Van91I CCANNNNNTGG 1 cut(s) 591
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 518
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 2 cut(s) 858, 1089
ZraI GACGTC 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.