Rh7DG016300

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0015046
Physical Location & Seq
Reverse (-)
1325331 .. 1330325
4995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG016300.1

Sequence Viewer

Length: 1164 bp
ATGTGGATGATGGGTTACAACGATGGTGGTGACTTCAACATGATTCCGGATTCAGCCTTCAACGGTCGGAAGCTTCGGCCTCTCGTTCCTAGGCCTCTGTCATCTCCTCATAACAACAACAACAACAACAACACTTCAACAAGTACACATGCTCCACCTTGTTTCAGTAGAATTCATGCTGCGTCTGATTTCTTAGCACAATATCACCAGCTGGGTGAACAAAGAGGGATGAGAGAGTTCAATACGGTGCCGCAGGTTGTGGTGAGCTCGAGATGGAACCCCACGCCGGAACAGTTGAGGGCACTAGAGGAGTTATATCGACGAGGGACTCGAACCCCATCGGCTGAGCAGATTCAGCAAATCACTGCTCAGCTTAGAAGGTATGGAAAGATAGAGGGCAAGAACGTGTTCTATTGGTTTCAAAACCACAAGGCAAGAGAGAGACAGAAACGCCGTCGCCAGATGGAATCTCACGACGACCTTCAACTCATGTGTTCAGATCATCAAAAGAAGGAATCATTAGGCGGGGCAAGTAGGACAGTGTATGAAGTTGAAGAGACCAAGAATTGGGCACCCTCTGCAAACTGCATGACTACTAGTCTCCAAGATCAGCTTCATCATCAACAGGAAACAGTAAAAGCAGGAGCAGAGAGCAGGACGGAAGCATGGGTCCAATTCGGTGAGGAAGAATTGCAGCAACTTCAACAAGAGCAAAGAAGGAATTCCATGACTGTAGAAAAGAATGACAGGTGGCAGATGATGCAGTTGTCTTGTCCTCCTCCTCCTCGTCTTCATGACCTCACCCAACTCATAAACGCCCCCACTTGTACCAGAACGACAGTAAGAACAATGGAGATGGACCAAAAGCAGCTCATTAATAACCGTAATAATAAGACTAGTCCTCCTCATGATCTCAGCATCTTTATAGCTCCCTACAGGTCTGGCGCTGCCCTTAACGGTCGCCATCTCCAACAAAACCAATACCGTAACGAAGAAGAAGATATTTCACGTGGAGATCAGTCTCAAACCCTCCAGCTATTTCCGCTTCGGAGTAGTACTTGCGGCGAAAAAGATCATCTTACAACCGAGAAATCCTTGCTAAACCCTAGTTTCGGCACTGGCCCTAATTACCAGTTTTTTGAGTTCCTCCCATTGAAGAACTGA

Protein Analysis

387

Amino Acids

44.83

Weight (kDa)

8.68

Isoelectric Point (pI)

62.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 90 - 149 1.8e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015046)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18010
fragaria_vesca FvH4_5g17270
malus_domestica MD06G1231300.v1.1 MD14G1235800.v1.1
prunus_persica Prupe.5G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178511
rosa_laevigata RLG00000005441
rosa_multiflora Rmu_sc0002421.1_g000001 Rmu_sc0016060.1_g000002
rosa_roxburghii Rroxscaffold_3G00274850
rosa_rugosa Rorug06G0417700
rosa_samantha Rh7AG016200 Rh7BG015700 Rh7CG016400 Rh7DG016300
rosa_wichuraiana Rw7G001280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 2 cut(s) 247, 571
AccB7I CCANNNNNTGG 1 cut(s) 567
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 4 cut(s) 251, 525, 1043, 1062
AcsI RAATTY 2 cut(s) 171, 721
AcvI CACGTG 1 cut(s) 1010
AfaI GTAC 3 cut(s) 145, 829, 1057
AfiI CCNNNNNNNGG 3 cut(s) 286, 567, 1112
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 9 cut(s) 37, 61, 138, 241, 422, 485, 554, 704, 1156
AhdI GACNNNNNGTC 1 cut(s) 597
AhlI ACTAGT 2 cut(s) 596, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 8 cut(s) 73, 211, 267, 373, 613, 871, 929, 1036
AluI AGCT 8 cut(s) 73, 211, 267, 373, 613, 871, 929, 1036
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 4 cut(s) 436, 551, 605, 1026
Ama87I CYCGRG 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 3 cut(s) 77, 92, 1120
ApeKI GCWGC 4 cut(s) 179, 694, 868, 947
ApoI RAATTY 2 cut(s) 171, 721
AseI ATTAAT 1 cut(s) 876
Asp700I GAANNNNTTC 2 cut(s) 407, 721
AspA2I CCTAGG 1 cut(s) 89
AspLEI GCGC 1 cut(s) 947
AspS9I GGNCC 3 cut(s) 670, 859, 1121
AsuHPI GGTGA 6 cut(s) 41, 197, 227, 274, 692, 793
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 2 cut(s) 670, 859
AvrII CCTAGG 1 cut(s) 89
BaeGI GKGCMC 2 cut(s) 304, 574
BanI GGYRCC 2 cut(s) 247, 571
BanII GRGCYC 1 cut(s) 269
BbrPI CACGTG 1 cut(s) 1010
BbsI GAAGAC 1 cut(s) 782
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 4 cut(s) 166, 706, 880, 934
BccI CCATC 7 cut(s) 4, 17, 267, 346, 457, 850, 972
BceAI ACGGC 1 cut(s) 438
BcoDI GTCTC 4 cut(s) 436, 551, 605, 1026
BcuI ACTAGT 2 cut(s) 596, 896
BfaI CTAG 5 cut(s) 90, 305, 597, 897, 1107
BfmI CTRYAG 2 cut(s) 732, 934
BfoI RGCGCY 1 cut(s) 948
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 6 cut(s) 180, 251, 695, 869, 948, 1063
BlnI CCTAGG 1 cut(s) 89
BlpI GCTNAGC 2 cut(s) 345, 369
BlsI GCNGC 6 cut(s) 181, 252, 696, 870, 949, 1064
BmcAI AGTACT 1 cut(s) 1057
Bme18I GGWCC 2 cut(s) 670, 859
BmeRI GACNNNNNGTC 1 cut(s) 597
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 670, 859, 1121
BmiI GGNNCC 4 cut(s) 249, 278, 573, 671
BmsI GCATC 2 cut(s) 750, 927
BpiI GAAGAC 1 cut(s) 782
BpmI CTGGAG 1 cut(s) 1016
Bpu1102I GCTNAGC 2 cut(s) 345, 369
BsaAI YACGTR 1 cut(s) 1010
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 886, 916
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 567, 1112
Bse1I ACTGG 2 cut(s) 1123, 1132
BseAI TCCGGA 1 cut(s) 46
BseDI CCNNGG 1 cut(s) 89
BseGI GGATG 2 cut(s) 12, 234
BseLI CCNNNNNNNGG 3 cut(s) 286, 567, 1112
BseMII CTCAG 3 cut(s) 336, 383, 928
BseNI ACTGG 2 cut(s) 1123, 1132
BseRI GAGGAG 6 cut(s) 96, 323, 768, 771, 774, 894
BseSI GKGCMC 2 cut(s) 304, 574
BseXI GCAGC 4 cut(s) 166, 706, 880, 934
BseYI CCCAGC 1 cut(s) 211
Bsh1285I CGRYCG 2 cut(s) 67, 961
BshFI GGCC 3 cut(s) 79, 94, 1122
BshNI GGYRCC 2 cut(s) 247, 571
BsiEI CGRYCG 2 cut(s) 67, 961
BsiHKAI GWGCWC 1 cut(s) 269
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 2 cut(s) 47, 287
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 286, 567, 1112
BsmAI GTCTC 4 cut(s) 436, 551, 605, 1026
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 3 cut(s) 79, 94, 1122
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 269, 304, 574
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 5 cut(s) 499, 607, 910, 1015, 1072
Bsp1720I GCTNAGC 2 cut(s) 345, 369
BspACI CCGC 4 cut(s) 251, 525, 1043, 1062
BspANI GGCC 3 cut(s) 79, 94, 1122
BspCNI CTCAG 3 cut(s) 337, 382, 927
BspEI TCCGGA 1 cut(s) 46
BspHI TCATGA 2 cut(s) 793, 907
BspLI GGNNCC 4 cut(s) 249, 278, 573, 671
BspMI ACCTGC 1 cut(s) 244
BspT107I GGYRCC 2 cut(s) 247, 571
BspTNI GGTCTC 1 cut(s) 551
BsrI ACTGG 2 cut(s) 1123, 1132
BssECI CCNNGG 1 cut(s) 89
BssMI GATC 5 cut(s) 499, 607, 910, 1015, 1072
BssT1I CCWWGG 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 549
BstAPI GCANNNNNTGC 2 cut(s) 578, 760
BstBAI YACGTR 1 cut(s) 1010
BstDEI CTNAG 5 cut(s) 193, 345, 369, 374, 914
BstF5I GGATG 2 cut(s) 12, 234
BstH2I RGCGCY 1 cut(s) 948
BstHHI GCGC 1 cut(s) 947
BstKTI GATC 5 cut(s) 502, 610, 913, 1018, 1075
BstMAI GTCTC 4 cut(s) 436, 551, 605, 1026
BstMBI GATC 5 cut(s) 499, 607, 910, 1015, 1072
BstMCI CGRYCG 2 cut(s) 67, 961
BstMWI GCNNNNNNNGC 4 cut(s) 355, 578, 760, 1042
BstNSI RCATGY 1 cut(s) 152
BstSFI CTRYAG 2 cut(s) 732, 934
BstSLI GKGCMC 2 cut(s) 304, 574
BstV1I GCAGC 4 cut(s) 166, 706, 880, 934
BstV2I GAAGAC 1 cut(s) 782
BsuRI GGCC 3 cut(s) 79, 94, 1122
BtsCI GGATG 2 cut(s) 12, 234
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 363, 546, 1116
BveI ACCTGC 1 cut(s) 244
CciI TCATGA 2 cut(s) 793, 907
CfoI GCGC 1 cut(s) 947
Cfr13I GGNCC 3 cut(s) 670, 859, 1121
CseI GACGC 1 cut(s) 171
Csp6I GTAC 3 cut(s) 144, 828, 1056
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 9 cut(s) 40, 149, 176, 490, 589, 666, 727, 794, 908
CviQI GTAC 3 cut(s) 144, 828, 1056
DdeI CTNAG 5 cut(s) 193, 345, 369, 374, 914
DpnI GATC 5 cut(s) 501, 609, 912, 1017, 1074
DpnII GATC 5 cut(s) 499, 607, 910, 1015, 1072
DriI GACNNNNNGTC 1 cut(s) 597
Eam1104I CTCTTC 1 cut(s) 549
Eam1105I GACNNNNNGTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 549
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 89
Eco147I AGGCCT 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 2 cut(s) 670, 859
Eco53kI GAGCTC 1 cut(s) 267
Eco72I CACGTG 1 cut(s) 1010
Eco88I CYCGRG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 267
EcoRI GAATTC 2 cut(s) 171, 721
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 9 cut(s) 43, 152, 179, 493, 592, 669, 730, 797, 911
FalI AAGNNNNNCTT 4 cut(s) 597, 629, 1062, 1094
FaqI GGGAC 1 cut(s) 340
FatI CATG 9 cut(s) 39, 148, 175, 489, 588, 665, 726, 793, 907
FauI CCCGC 1 cut(s) 518
Fnu4HI GCNGC 6 cut(s) 180, 251, 695, 869, 948, 1063
FokI GGATG 2 cut(s) 19, 241
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 6 cut(s) 180, 251, 695, 869, 948, 1063
FspBI CTAG 5 cut(s) 90, 305, 597, 897, 1107
GlaI GCGC 1 cut(s) 946
GluI GCNGC 6 cut(s) 180, 251, 695, 869, 948, 1063
GsaI CCCAGC 1 cut(s) 215
GsuI CTGGAG 1 cut(s) 1016
HaeII RGCGCY 1 cut(s) 948
HaeIII GGCC 3 cut(s) 79, 94, 1122
HapII CCGG 2 cut(s) 47, 287
HgaI GACGC 1 cut(s) 171
HhaI GCGC 1 cut(s) 947
Hin1II CATG 9 cut(s) 43, 152, 179, 493, 592, 669, 730, 797, 911
Hin6I GCGC 1 cut(s) 945
HinP1I GCGC 1 cut(s) 945
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 6 cut(s) 43, 50, 328, 352, 467, 515
HpaII CCGG 2 cut(s) 47, 287
HphI GGTGA 6 cut(s) 41, 197, 227, 274, 692, 793
Hpy166II GTNNAC 2 cut(s) 146, 218
Hpy188I TCNGA 4 cut(s) 69, 187, 499, 1050
Hpy188III TCNNGA 5 cut(s) 47, 270, 473, 794, 908
Hpy8I GTNNAC 2 cut(s) 146, 218
Hpy99I CGWCG 3 cut(s) 324, 459, 479
HpyAV CCTTC 5 cut(s) 67, 372, 491, 505, 711
HpyCH4IV ACGT 2 cut(s) 405, 1009
HpyCH4V TGCA 4 cut(s) 581, 588, 694, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 355, 578, 760, 1042
HpyF3I CTNAG 5 cut(s) 193, 345, 369, 374, 914
HpySE526I ACGT 2 cut(s) 405, 1009
Hsp92II CATG 9 cut(s) 43, 152, 179, 493, 592, 669, 730, 797, 911
HspAI GCGC 1 cut(s) 945
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 5 cut(s) 499, 607, 910, 1015, 1072
LmnI GCTCC 3 cut(s) 157, 644, 934
Lsp1109I GCAGC 4 cut(s) 166, 706, 880, 934
LweI GCATC 2 cut(s) 750, 927
MaeI CTAG 5 cut(s) 90, 305, 597, 897, 1107
MaeII ACGT 2 cut(s) 405, 1009
MaeIII GTNAC 3 cut(s) 14, 29, 986
MalI GATC 5 cut(s) 501, 609, 912, 1017, 1074
MboI GATC 5 cut(s) 499, 607, 910, 1015, 1072
MboII GAAGA 6 cut(s) 566, 698, 782, 1004, 1007, 1010
MhlI GDGCHC 3 cut(s) 269, 304, 574
MluCI AATT 6 cut(s) 171, 565, 674, 689, 721, 1126
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 2 cut(s) 47, 994
MroI TCCGGA 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 407, 721
MseI TTAA 2 cut(s) 876, 954
MspA1I CMGCKG 1 cut(s) 211
MspI CCGG 2 cut(s) 47, 287
MwoI GCNNNNNNNGC 4 cut(s) 355, 578, 760, 1042
NdeII GATC 5 cut(s) 499, 607, 910, 1015, 1072
NlaIII CATG 9 cut(s) 43, 152, 179, 493, 592, 669, 730, 797, 911
NlaIV GGNNCC 4 cut(s) 249, 278, 573, 671
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 152
PaeR7I CTCGAG 1 cut(s) 268
PagI TCATGA 2 cut(s) 793, 907
PceI AGGCCT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PdmI GAANNNNTTC 2 cut(s) 407, 721
PfeI GAWTC 5 cut(s) 43, 50, 352, 467, 515
PflMI CCANNNNNTGG 1 cut(s) 567
PkrI GCNGC 6 cut(s) 181, 252, 696, 870, 949, 1064
PleI GAGTC 1 cut(s) 322
PmaCI CACGTG 1 cut(s) 1010
PmlI CACGTG 1 cut(s) 1010
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 1010
PshBI ATTAAT 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 269
PspCI CACGTG 1 cut(s) 1010
PspFI CCCAGC 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 249, 278, 573, 671
PspPI GGNCC 3 cut(s) 670, 859, 1121
PvuII CAGCTG 1 cut(s) 211
RsaI GTAC 3 cut(s) 145, 829, 1057
RsaNI GTAC 3 cut(s) 144, 828, 1056
SacI GAGCTC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 876, 954
SatI GCNGC 6 cut(s) 180, 251, 695, 869, 948, 1063
Sau3AI GATC 5 cut(s) 499, 607, 910, 1015, 1072
Sau96I GGNCC 3 cut(s) 670, 859, 1121
ScaI AGTACT 1 cut(s) 1057
SchI GAGTC 1 cut(s) 322
SduI GDGCHC 3 cut(s) 269, 304, 574
SfaNI GCATC 2 cut(s) 750, 927
SfcI CTRYAG 2 cut(s) 732, 934
Sfr274I CTCGAG 1 cut(s) 268
SinI GGWCC 2 cut(s) 670, 859
SlaI CTCGAG 1 cut(s) 268
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
SpeI ACTAGT 2 cut(s) 596, 896
Sse9I AATT 6 cut(s) 171, 565, 674, 689, 721, 1126
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 4 cut(s) 251, 525, 1043, 1062
SspMI CTAG 5 cut(s) 90, 305, 597, 897, 1107
SstI GAGCTC 1 cut(s) 269
StuI AGGCCT 1 cut(s) 94
StyI CCWWGG 1 cut(s) 89
TaiI ACGT 2 cut(s) 408, 1012
TaqI TCGA 3 cut(s) 269, 319, 331
TasI AATT 6 cut(s) 171, 565, 674, 689, 721, 1126
TatI WGTACW 2 cut(s) 143, 1055
TauI GCSGC 2 cut(s) 253, 1065
TfiI GAWTC 5 cut(s) 43, 50, 352, 467, 515
Tru1I TTAA 2 cut(s) 876, 954
Tru9I TTAA 2 cut(s) 876, 954
TscAI CASTG 3 cut(s) 370, 546, 1123
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 4 cut(s) 179, 694, 868, 947
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 4 cut(s) 164, 561, 605, 782
TspGWI ACGGA 1 cut(s) 674
TspRI CASTG 3 cut(s) 370, 546, 1123
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 670, 859
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 2 cut(s) 171, 721
XceI RCATGY 1 cut(s) 152
XhoI CTCGAG 1 cut(s) 268
XmaJI CCTAGG 1 cut(s) 89
XmnI GAANNNNTTC 2 cut(s) 407, 721
XspI CTAG 5 cut(s) 90, 305, 597, 897, 1107
ZrmI AGTACT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.