MD14G1235800.v1.1

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015046
Physical Location & Seq
Reverse (-)
31578663 .. 31582452
3790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1235800.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGTGGATGATGGGTTACAATGACGGTGGCCATGGCGATTTCAACATGCCGGATTCTTTCAATGGACGGAAGCTCCGCCCTCTCGTTCCGAGGCCACTTCCATCTGTAAACAACACAGTTTCTTCAACCATCACAACTCCTCCTTGTCTAAACCGATCAGCAATTCATGGCTCTCACGATTTTTTTCCGCAGTATCACCAACTCGCCGCTATGGCTGAACAAAACAAGAGATCGGAGTTTCTCAACACGCCACAGGTTGTAGTGAGCTCAAGATGGAACCCTACGCCGGAGCAGTTGAGGGCTCTTGAAGAATTATACAGACGAGGGACTCGAACACCGTCCGCGGAGCAGATTCAGCACATCACCGCTCAGCTTCGGAAATATGGAAAGATAGAGGGGAAGAATGTGTTCTATTGGTTCCAAAACCACAAGGCCAGAGAAAGGCAGAAACGTCGCCGTCAAATGGAATCATCTGCCCTCTCTGCAGATCACGACCAGCCACTGCAAATATCAATGACGTCTGCAGATAATCAAAAGAAAGAAGAATCATTAGGGGCAAGTATGACAGGGTTTGAAGTTGAAGAGACCAAGAACTGGGCACCCTTTACAAACTGTAGTACTACTACTCTTGCTGAGGAATCTGTTTCAATACAAAGGGCAGCAAAAGCAAGAATGGCAGCAGCGGACTGCAGGGCGGATGGATGGATCCAATTCGACGAAGAAGAATTGCAGCAAAGAAGAAACTTTGTAGAAAGGAATGCCACGTGGCAGATGATGCAGTTGTCTTGTCCTTCTCACCCTCACCCTCCCACCCACCTCATAAACACCCCTCCTACTTGTGCTAGTACGACAACCAGATCTAGGGCAGCAGCTACAGGAAGAACAATGGACCAAAGGCTCATTAAGTCTCATGATCTCAGCATCTGTATAGCACCCCACGCATCAGAAACTGGTTTCAACATTGAGCAAGATCATGGATGTGAGGAATCTCAAACCCTTCAACTCTTTCCGCTTCGAAGCGGTCATGCCAATGCCAGTGCCAATACCAATGAATTAAGCATTAGCGATAAGGATCATGCGGAGCTATCGGCTAGCTTCAGTGCTCATCCTTACTCCTTTTTTGAGTTCCTTCCATTAAAGAATTAA

Protein Analysis

382

Amino Acids

43.06

Weight (kDa)

6.97

Isoelectric Point (pI)

56.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 90 - 149 2e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015046)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18010
fragaria_vesca FvH4_5g17270
malus_domestica MD06G1231300.v1.1 MD14G1235800.v1.1
prunus_persica Prupe.5G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178511
rosa_laevigata RLG00000005441
rosa_multiflora Rmu_sc0002421.1_g000001 Rmu_sc0016060.1_g000002
rosa_roxburghii Rroxscaffold_3G00274850
rosa_rugosa Rorug06G0417700
rosa_samantha Rh7AG016200 Rh7BG015700 Rh7CG016400 Rh7DG016300
rosa_wichuraiana Rw7G001280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 598
AccB7I CCANNNNNTGG 1 cut(s) 594
AccBSI CCGCTC 1 cut(s) 368
AccII CGCG 1 cut(s) 344
AclWI GGATC 3 cut(s) 700, 713, 1080
AcoI YGGCCR 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 1081
AcvI CACGTG 1 cut(s) 765
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 2 cut(s) 619, 847
AfiI CCNNNNNNNGG 5 cut(s) 286, 441, 463, 594, 861
AgsI TTSAA 9 cut(s) 43, 61, 126, 308, 575, 581, 648, 958, 1001
AluBI AGCT 6 cut(s) 73, 267, 373, 872, 1084, 1095
AluI AGCT 6 cut(s) 73, 267, 373, 872, 1084, 1095
Alw21I GWGCWC 2 cut(s) 269, 1105
Alw26I GTCTC 2 cut(s) 578, 912
AlwI GGATC 3 cut(s) 700, 713, 1080
AlwNI CAGNNNCTG 3 cut(s) 502, 924, 950
AoxI GGCC 3 cut(s) 28, 92, 432
ApeKI GCWGC 6 cut(s) 659, 677, 680, 730, 866, 869
Asp700I GAANNNNTTC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 889
AsuHPI GGTGA 4 cut(s) 188, 355, 788, 794
AsuII TTCGAA 1 cut(s) 1015
AsuNHI GCTAGC 1 cut(s) 1091
AvaII GGWCC 1 cut(s) 889
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 705
BanI GGYRCC 1 cut(s) 598
BanII GRGCYC 2 cut(s) 269, 304
BbrPI CACGTG 1 cut(s) 765
Bbv12I GWGCWC 2 cut(s) 269, 1105
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 6 cut(s) 671, 689, 692, 742, 878, 881
BccI CCATC 6 cut(s) 4, 109, 137, 267, 692, 696
BceAI ACGGC 1 cut(s) 441
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BcoDI GTCTC 2 cut(s) 578, 912
BfaI CTAG 3 cut(s) 843, 861, 1092
BfmI CTRYAG 5 cut(s) 483, 522, 613, 688, 873
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 1 cut(s) 857
BisI GCNGC 7 cut(s) 207, 660, 678, 681, 731, 867, 870
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 7 cut(s) 208, 661, 679, 682, 732, 868, 871
BmcAI AGTACT 1 cut(s) 619
Bme18I GGWCC 1 cut(s) 889
BmgT120I GGNCC 1 cut(s) 889
BmiI GGNNCC 4 cut(s) 278, 419, 600, 707
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 3 cut(s) 765, 930, 950
BmtI GCTAGC 1 cut(s) 1095
BmuI ACTGGG 1 cut(s) 604
Bpu10I CCTNAGC 1 cut(s) 633
Bpu1102I GCTNAGC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 1015
BpuEI CTTGAG 1 cut(s) 253
BsaAI YACGTR 1 cut(s) 765
BsaBI GATNNNNATC 1 cut(s) 1071
BsaHI GRCGYC 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 3 cut(s) 31, 89, 342
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 124, 154
Bsc4I CCNNNNNNNGG 5 cut(s) 286, 441, 463, 594, 861
Bse1I ACTGG 3 cut(s) 599, 955, 1035
Bse8I GATNNNNATC 1 cut(s) 1071
BseDI CCNNGG 3 cut(s) 31, 89, 342
BseGI GGATG 5 cut(s) 12, 703, 707, 983, 1105
BseJI GATNNNNATC 1 cut(s) 1071
BseLI CCNNNNNNNGG 5 cut(s) 286, 441, 463, 594, 861
BseMII CTCAG 3 cut(s) 383, 624, 931
BseNI ACTGG 3 cut(s) 599, 955, 1035
BseRI GAGGAG 1 cut(s) 129
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 6 cut(s) 671, 689, 692, 742, 878, 881
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 30, 94, 434
BshNI GGYRCC 1 cut(s) 598
BsiHKAI GWGCWC 2 cut(s) 269, 1105
BsiSI CCGG 2 cut(s) 50, 287
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 5 cut(s) 286, 441, 463, 594, 861
BsmAI GTCTC 2 cut(s) 578, 912
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 3 cut(s) 30, 94, 434
Bso31I GGTCTC 1 cut(s) 578
Bsp119I TTCGAA 1 cut(s) 1015
Bsp1286I GDGCHC 4 cut(s) 269, 304, 601, 1105
Bsp143I GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
Bsp1720I GCTNAGC 1 cut(s) 369
Bsp19I CCATGG 1 cut(s) 31
BspANI GGCC 3 cut(s) 30, 94, 434
BspCNI CTCAG 3 cut(s) 382, 625, 930
BspFNI CGCG 1 cut(s) 344
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 4 cut(s) 278, 419, 600, 707
BspMAI CTGCAG 3 cut(s) 487, 526, 692
BspOI GCTAGC 1 cut(s) 1095
BspPI GGATC 3 cut(s) 700, 713, 1080
BspT104I TTCGAA 1 cut(s) 1015
BspT107I GGYRCC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 578
BsrBI CCGCTC 1 cut(s) 368
BsrI ACTGG 3 cut(s) 599, 955, 1035
BssECI CCNNGG 3 cut(s) 31, 89, 342
BssMI GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
BssNI GRCGYC 1 cut(s) 518
BssT1I CCWWGG 1 cut(s) 31
Bst4CI ACNGT 4 cut(s) 26, 118, 339, 614
Bst6I CTCTTC 1 cut(s) 576
BstACI GRCGYC 1 cut(s) 518
BstAPI GCANNNNNTGC 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 765
BstBI TTCGAA 1 cut(s) 1015
BstC8I GCNNGC 1 cut(s) 1093
BstDEI CTNAG 3 cut(s) 369, 633, 917
BstDSI CCRYGG 2 cut(s) 31, 342
BstF5I GGATG 5 cut(s) 12, 703, 707, 983, 1105
BstFNI CGCG 1 cut(s) 344
BstKTI GATC 8 cut(s) 158, 233, 490, 708, 860, 916, 973, 1075
BstMAI GTCTC 2 cut(s) 578, 912
BstMBI GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
BstMWI GCNNNNNNNGC 7 cut(s) 212, 355, 482, 665, 674, 775, 938
BstNSI RCATGY 1 cut(s) 49
BstSFI CTRYAG 5 cut(s) 483, 522, 613, 688, 873
BstSLI GKGCMC 1 cut(s) 601
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 6 cut(s) 671, 689, 692, 742, 878, 881
BstX2I RGATCY 2 cut(s) 705, 857
BstYI RGATCY 2 cut(s) 705, 857
BsuRI GGCC 3 cut(s) 30, 94, 434
BtgI CCRYGG 2 cut(s) 31, 342
BtsCI GGATG 5 cut(s) 12, 703, 707, 983, 1105
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 3 cut(s) 500, 1042, 1105
Cac8I GCNNGC 1 cut(s) 1093
CaiI CAGNNNCTG 3 cut(s) 502, 924, 950
CciI TCATGA 1 cut(s) 910
Cfr13I GGNCC 1 cut(s) 889
Cfr42I CCGCGG 1 cut(s) 345
Csp6I GTAC 2 cut(s) 618, 846
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 7 cut(s) 32, 46, 167, 911, 974, 1025, 1076
CviQI GTAC 2 cut(s) 618, 846
DdeI CTNAG 3 cut(s) 369, 633, 917
DpnI GATC 8 cut(s) 157, 232, 489, 707, 859, 915, 972, 1074
DpnII GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
EciI GGCGGA 2 cut(s) 65, 710
Ecl136II GAGCTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 269, 304
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 1081
Eco72I CACGTG 1 cut(s) 765
EcoICRI GAGCTC 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 269, 304
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 7 cut(s) 35, 49, 170, 914, 977, 1028, 1079
FaqI GGGAC 1 cut(s) 340
FatI CATG 7 cut(s) 31, 45, 166, 910, 973, 1024, 1075
Fnu4HI GCNGC 7 cut(s) 207, 660, 678, 681, 731, 867, 870
FokI GGATG 5 cut(s) 19, 710, 714, 990, 1092
FriOI GRGCYC 2 cut(s) 269, 304
Fsp4HI GCNGC 7 cut(s) 207, 660, 678, 681, 731, 867, 870
FspBI CTAG 3 cut(s) 843, 861, 1092
GluI GCNGC 7 cut(s) 207, 660, 678, 681, 731, 867, 870
HaeIII GGCC 3 cut(s) 30, 94, 434
HapII CCGG 2 cut(s) 50, 287
Hin1I GRCGYC 1 cut(s) 518
Hin1II CATG 7 cut(s) 35, 49, 170, 914, 977, 1028, 1079
HinfI GANTC 7 cut(s) 53, 328, 352, 467, 545, 638, 986
HpaII CCGG 2 cut(s) 50, 287
HphI GGTGA 4 cut(s) 188, 355, 788, 794
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 90, 235, 378, 946
Hpy188III TCNNGA 5 cut(s) 176, 270, 305, 491, 911
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 2 cut(s) 456, 719
HpyAV CCTTC 3 cut(s) 801, 1007, 1139
HpyCH4III ACNGT 4 cut(s) 26, 118, 339, 614
HpyCH4IV ACGT 3 cut(s) 451, 518, 764
HpyCH4V TGCA 6 cut(s) 485, 505, 524, 690, 730, 778
HpyF10VI GCNNNNNNNGC 7 cut(s) 212, 355, 482, 665, 674, 775, 938
HpyF3I CTNAG 3 cut(s) 369, 633, 917
HpySE526I ACGT 3 cut(s) 451, 518, 764
Hsp92I GRCGYC 1 cut(s) 518
Hsp92II CATG 7 cut(s) 35, 49, 170, 914, 977, 1028, 1079
KspI CCGCGG 1 cut(s) 345
Kzo9I GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
LmnI GCTCC 4 cut(s) 78, 289, 346, 1081
Lsp1109I GCAGC 6 cut(s) 671, 689, 692, 742, 878, 881
LweI GCATC 3 cut(s) 765, 930, 950
MaeI CTAG 3 cut(s) 843, 861, 1092
MaeII ACGT 3 cut(s) 451, 518, 764
MaeIII GTNAC 1 cut(s) 14
MalI GATC 8 cut(s) 157, 232, 489, 707, 859, 915, 972, 1074
MbiI CCGCTC 1 cut(s) 368
MboI GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
MboII GAAGA 9 cut(s) 114, 320, 412, 554, 593, 731, 734, 750, 891
MflI RGATCY 2 cut(s) 705, 857
MhlI GDGCHC 4 cut(s) 269, 304, 601, 1105
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 6 cut(s) 162, 311, 710, 725, 1052, 1141
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 1 cut(s) 322
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 407
MscI TGGCCA 1 cut(s) 30
MseI TTAA 4 cut(s) 903, 1055, 1136, 1144
MslI CAYNNNNRTG 2 cut(s) 978, 1029
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 2 cut(s) 344, 683
MspI CCGG 2 cut(s) 50, 287
Mva1269I GAATGC 1 cut(s) 763
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 7 cut(s) 212, 355, 482, 665, 674, 775, 938
NcoI CCATGG 1 cut(s) 31
NdeII GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
NheI GCTAGC 1 cut(s) 1091
NlaIII CATG 7 cut(s) 35, 49, 170, 914, 977, 1028, 1079
NlaIV GGNNCC 4 cut(s) 278, 419, 600, 707
NspI RCATGY 1 cut(s) 49
NspV TTCGAA 1 cut(s) 1015
PagI TCATGA 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PctI GAATGC 1 cut(s) 763
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 6 cut(s) 53, 352, 467, 545, 638, 986
PflMI CCANNNNNTGG 1 cut(s) 594
PkrI GCNGC 7 cut(s) 208, 661, 679, 682, 732, 868, 871
PleI GAGTC 1 cut(s) 322
PmaCI CACGTG 1 cut(s) 765
PmlI CACGTG 1 cut(s) 765
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 765
Psp124BI GAGCTC 1 cut(s) 269
PspCI CACGTG 1 cut(s) 765
PspN4I GGNNCC 4 cut(s) 278, 419, 600, 707
PspPI GGNCC 1 cut(s) 889
PstI CTGCAG 3 cut(s) 487, 526, 692
PstNI CAGNNNCTG 3 cut(s) 502, 924, 950
PsuI RGATCY 2 cut(s) 705, 857
RsaI GTAC 2 cut(s) 619, 847
RsaNI GTAC 2 cut(s) 618, 846
RseI CAYNNNNRTG 2 cut(s) 978, 1029
SacI GAGCTC 1 cut(s) 269
SacII CCGCGG 1 cut(s) 345
SaqAI TTAA 4 cut(s) 903, 1055, 1136, 1144
SatI GCNGC 7 cut(s) 207, 660, 678, 681, 731, 867, 870
Sau3AI GATC 8 cut(s) 155, 230, 487, 705, 857, 913, 970, 1072
Sau96I GGNCC 1 cut(s) 889
ScaI AGTACT 1 cut(s) 619
SchI GAGTC 1 cut(s) 322
SduI GDGCHC 4 cut(s) 269, 304, 601, 1105
SfaNI GCATC 3 cut(s) 765, 930, 950
SfcI CTRYAG 5 cut(s) 483, 522, 613, 688, 873
Sfr303I CCGCGG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 1015
SgrBI CCGCGG 1 cut(s) 345
SinI GGWCC 1 cut(s) 889
SmiMI CAYNNNNRTG 2 cut(s) 978, 1029
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 6 cut(s) 162, 311, 710, 725, 1052, 1141
SspMI CTAG 3 cut(s) 843, 861, 1092
SstI GAGCTC 1 cut(s) 269
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 4 cut(s) 26, 118, 339, 614
TaiI ACGT 3 cut(s) 454, 521, 767
TaqI TCGA 3 cut(s) 331, 714, 1015
TasI AATT 6 cut(s) 162, 311, 710, 725, 1052, 1141
TatI WGTACW 1 cut(s) 617
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 6 cut(s) 53, 352, 467, 545, 638, 986
Tru1I TTAA 4 cut(s) 903, 1055, 1136, 1144
Tru9I TTAA 4 cut(s) 903, 1055, 1136, 1144
TscAI CASTG 3 cut(s) 507, 1042, 1105
TseI GCWGC 6 cut(s) 659, 677, 680, 730, 866, 869
TspDTI ATGAA 2 cut(s) 155, 1065
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 3 cut(s) 507, 1042, 1105
Van91I CCANNNNNTGG 1 cut(s) 594
VpaK11BI GGWCC 1 cut(s) 889
XceI RCATGY 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 3 cut(s) 843, 861, 1092
ZraI GACGTC 1 cut(s) 519
ZrmI AGTACT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.