RLG00000005441

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015046
Physical Location & Seq
Forward (+)
67649048 .. 67651313
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005441

Sequence Viewer

Length: 1221 bp
ATGTGGATGATGGGTTATAACGATGGTGGTGACTTCAACATGATTCCGGATTCAACCTTCAACGGTCGGAAGCTTCGGCCTCTCGTTCCTAGGCCTCTGTCATCTCCTCATAACAACAACAATAACAACAACAACAACACTTCAACAAGTACACATGCTCCACCTTGTTTCAGTCGAATTCATGCCGCGTCTGATTTCTTAGCACAATATCACCAGCTGGGTGAACAAAGAGGGATGAGAGAGTTCAATACGGTGCCGCAGGTTGTGGTGAGCTCGAGATGGAACCCCACGCCGGAACAGTTGAGGGCACTAGAGGAGTTATATCGACGAGGGACTCGAACCCCATCGGCTGAGCAGATTCAGCAAATCACTGCTCAGCTTAGAAGGTATGGAAAGATAGAGGGCAAGAACGTGTTCTATTGGTTCCAAAACCACAAGGCAAGAGAGAGACAGAAACGCCGTCGCCAGATGGAATCTCACGACGACCTTCAACTCATGTGTTCAGATCACCAAAAGAAGGAATCATTAGGCGGGGCAAGTAGGACAGTGTATGAAATTGAAGAGACCAAGAATTGTGCACCCTCTGCAAACTGCATGACTACTAGTCTCCAAGATCAGCTTCATCATCAACAGGTCTATTCTATTTCCCTATACCTGCTTCTGTTCCTGTCTTTGTTTTTGCAGGAAACAGTAAAAGCAGGAGCAGAGAGCAGGACGGAAGGATGGGTCCAATTCGGTGAGGAAGAATTGCAGCAACTTCAACAAGAGCAAAGAAGGAATTCCATGACTGTAGAAAAGAATGCCAGGTGGCAGATGATGCAGTTGTCTTGTCCTCCTCCTTCTCCTCTTCATGACCTCACCCACCTCATAAACGCCCCCACTTGTACCAGAACGACAGTAAGAACAATGGAGATGGACCAAAAGCAGCTCATTAATAACCGTAATAATAAGACTAGTCCTCCTCATGATCTCAGCATCTTTATAGCTCCCTACAGGTCTGGCGTTGCCCTTAACGGTCGCCATCTCCACCAAAACCAGCACCGTAACGAAGAAGAAGATATTTCACGTGGAGATCAGTCTCAAACCCTCCAGCTATTTCCGCTTCGGAGTAGTACTTGCGGCGAAAAAGATCATCTTACAACCGAGAAATCCTTGCTAAACCCTAGTTTCGGCACCGGCCCTAATTACCAGTTTTTTGAGTTCCTCCCATTGAAGAACTGA

Protein Analysis

407

Amino Acids

46.93

Weight (kDa)

8.62

Isoelectric Point (pI)

64.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 92 - 151 1.9e-15 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015046)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18010
fragaria_vesca FvH4_5g17270
malus_domestica MD06G1231300.v1.1 MD14G1235800.v1.1
prunus_persica Prupe.5G232600_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0178511
rosa_laevigata RLG00000005441
rosa_multiflora Rmu_sc0002421.1_g000001 Rmu_sc0016060.1_g000002
rosa_roxburghii Rroxscaffold_3G00274850
rosa_rugosa Rorug06G0417700
rosa_samantha Rh7AG016200 Rh7BG015700 Rh7CG016400 Rh7DG016300
rosa_wichuraiana Rw7G001280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 18
Acc36I ACCTGC 2 cut(s) 250, 663
AccB1I GGYRCC 2 cut(s) 253, 1172
AccII CGCG 1 cut(s) 188
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 5 cut(s) 186, 257, 531, 1100, 1119
AcsI RAATTY 2 cut(s) 177, 778
AcvI CACGTG 1 cut(s) 1067
AfaI GTAC 3 cut(s) 151, 886, 1114
AfiI CCNNNNNNNGG 3 cut(s) 292, 517, 1169
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 9 cut(s) 37, 54, 61, 144, 247, 491, 560, 761, 1213
AhdI GACNNNNNGTC 1 cut(s) 603
AhlI ACTAGT 2 cut(s) 602, 953
AjnI CCWGG 1 cut(s) 803
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
AluBI AGCT 8 cut(s) 73, 217, 273, 379, 619, 928, 986, 1093
AluI AGCT 8 cut(s) 73, 217, 273, 379, 619, 928, 986, 1093
Alw21I GWGCWC 2 cut(s) 275, 580
Alw26I GTCTC 4 cut(s) 442, 557, 611, 1083
Alw44I GTGCAC 1 cut(s) 576
Ama87I CYCGRG 1 cut(s) 274
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 3 cut(s) 77, 92, 1177
ApaLI GTGCAC 1 cut(s) 576
ApeKI GCWGC 2 cut(s) 751, 925
ApoI RAATTY 2 cut(s) 177, 778
AseI ATTAAT 1 cut(s) 933
Asp700I GAANNNNTTC 2 cut(s) 413, 778
AspA2I CCTAGG 1 cut(s) 89
AspS9I GGNCC 3 cut(s) 727, 916, 1178
AsuHPI GGTGA 7 cut(s) 41, 203, 233, 280, 500, 749, 850
AvaI CYCGRG 1 cut(s) 274
AvaII GGWCC 2 cut(s) 727, 916
AvrII CCTAGG 1 cut(s) 89
BaeGI GKGCMC 2 cut(s) 310, 580
BanI GGYRCC 2 cut(s) 253, 1172
BanII GRGCYC 1 cut(s) 275
BbrPI CACGTG 1 cut(s) 1067
Bbv12I GWGCWC 2 cut(s) 275, 580
BbvI GCAGC 2 cut(s) 763, 937
BccI CCATC 8 cut(s) 4, 17, 273, 352, 463, 717, 907, 1029
BceAI ACGGC 1 cut(s) 444
BciT130I CCWGG 1 cut(s) 805
BcoDI GTCTC 4 cut(s) 442, 557, 611, 1083
BcuI ACTAGT 2 cut(s) 602, 953
BfaI CTAG 5 cut(s) 90, 311, 603, 954, 1164
BfmI CTRYAG 2 cut(s) 789, 991
BfuAI ACCTGC 2 cut(s) 250, 663
BisI GCNGC 5 cut(s) 186, 257, 752, 926, 1120
BlnI CCTAGG 1 cut(s) 89
BlpI GCTNAGC 2 cut(s) 351, 375
BlsI GCNGC 5 cut(s) 187, 258, 753, 927, 1121
BmcAI AGTACT 1 cut(s) 1114
Bme1390I CCNGG 1 cut(s) 805
Bme18I GGWCC 2 cut(s) 727, 916
BmeRI GACNNNNNGTC 1 cut(s) 603
BmeT110I CYCGRG 1 cut(s) 274
BmgT120I GGNCC 3 cut(s) 727, 916, 1178
BmiI GGNNCC 5 cut(s) 255, 284, 425, 728, 1174
BmrFI CCNGG 1 cut(s) 805
BmsI GCATC 2 cut(s) 807, 984
BpmI CTGGAG 1 cut(s) 1073
Bpu1102I GCTNAGC 2 cut(s) 351, 375
BsaAI YACGTR 1 cut(s) 1067
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 943, 973
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 517, 1169
Bse118I RCCGGY 1 cut(s) 1175
Bse1I ACTGG 1 cut(s) 1189
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 1 cut(s) 805
BseDI CCNNGG 1 cut(s) 89
BseGI GGATG 3 cut(s) 12, 240, 728
BseLI CCNNNNNNNGG 3 cut(s) 292, 517, 1169
BseMII CTCAG 3 cut(s) 342, 389, 985
BseNI ACTGG 1 cut(s) 1189
BseRI GAGGAG 5 cut(s) 96, 329, 825, 834, 951
BseSI GKGCMC 2 cut(s) 310, 580
BseXI GCAGC 2 cut(s) 763, 937
BseYI CCCAGC 1 cut(s) 217
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 2 cut(s) 67, 1018
BshFI GGCC 3 cut(s) 79, 94, 1179
BshNI GGYRCC 2 cut(s) 253, 1172
BsiEI CGRYCG 2 cut(s) 67, 1018
BsiHKAI GWGCWC 2 cut(s) 275, 580
BsiHKCI CYCGRG 1 cut(s) 274
BsiSI CCGG 3 cut(s) 47, 293, 1176
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 3 cut(s) 292, 517, 1169
BsmAI GTCTC 4 cut(s) 442, 557, 611, 1083
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 3 cut(s) 79, 94, 1179
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 274
Bsp1286I GDGCHC 3 cut(s) 275, 310, 580
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 5 cut(s) 505, 613, 967, 1072, 1129
Bsp1720I GCTNAGC 2 cut(s) 351, 375
BspACI CCGC 5 cut(s) 186, 257, 531, 1100, 1119
BspANI GGCC 3 cut(s) 79, 94, 1179
BspCNI CTCAG 3 cut(s) 343, 388, 984
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 1 cut(s) 188
BspHI TCATGA 2 cut(s) 850, 964
BspLI GGNNCC 5 cut(s) 255, 284, 425, 728, 1174
BspMI ACCTGC 2 cut(s) 250, 663
BspT107I GGYRCC 2 cut(s) 253, 1172
BspTNI GGTCTC 1 cut(s) 557
BsrFI RCCGGY 1 cut(s) 1175
BsrI ACTGG 1 cut(s) 1189
BssAI RCCGGY 1 cut(s) 1175
BssECI CCNNGG 1 cut(s) 89
BssMI GATC 5 cut(s) 505, 613, 967, 1072, 1129
BssT1I CCWWGG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 805
Bst6I CTCTTC 2 cut(s) 555, 852
BstAPI GCANNNNNTGC 2 cut(s) 584, 817
BstBAI YACGTR 1 cut(s) 1067
BstDEI CTNAG 5 cut(s) 199, 351, 375, 380, 971
BstF5I GGATG 3 cut(s) 12, 240, 728
BstFNI CGCG 1 cut(s) 188
BstKTI GATC 5 cut(s) 508, 616, 970, 1075, 1132
BstMAI GTCTC 4 cut(s) 442, 557, 611, 1083
BstMBI GATC 5 cut(s) 505, 613, 967, 1072, 1129
BstMCI CGRYCG 2 cut(s) 67, 1018
BstMWI GCNNNNNNNGC 4 cut(s) 361, 584, 817, 1099
BstNI CCWGG 1 cut(s) 805
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 803
BstSFI CTRYAG 2 cut(s) 789, 991
BstSLI GKGCMC 2 cut(s) 310, 580
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 763, 937
BsuRI GGCC 3 cut(s) 79, 94, 1179
BtsCI GGATG 3 cut(s) 12, 240, 728
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 552
BveI ACCTGC 2 cut(s) 250, 663
CciI TCATGA 2 cut(s) 850, 964
Cfr10I RCCGGY 1 cut(s) 1175
Cfr13I GGNCC 3 cut(s) 727, 916, 1178
CseI GACGC 1 cut(s) 177
Csp6I GTAC 3 cut(s) 150, 885, 1113
CviAII CATG 8 cut(s) 40, 155, 182, 496, 595, 784, 851, 965
CviQI GTAC 3 cut(s) 150, 885, 1113
DdeI CTNAG 5 cut(s) 199, 351, 375, 380, 971
DpnI GATC 5 cut(s) 507, 615, 969, 1074, 1131
DpnII GATC 5 cut(s) 505, 613, 967, 1072, 1129
DriI GACNNNNNGTC 1 cut(s) 603
Eam1104I CTCTTC 2 cut(s) 555, 852
Eam1105I GACNNNNNGTC 1 cut(s) 603
EarI CTCTTC 2 cut(s) 555, 852
Ecl136II GAGCTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 89
Eco147I AGGCCT 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 727, 916
Eco53kI GAGCTC 1 cut(s) 273
Eco72I CACGTG 1 cut(s) 1067
Eco88I CYCGRG 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 273
EcoRI GAATTC 2 cut(s) 177, 778
EcoRII CCWGG 1 cut(s) 803
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 8 cut(s) 43, 158, 185, 499, 598, 787, 854, 968
FalI AAGNNNNNCTT 4 cut(s) 603, 635, 1119, 1151
FaqI GGGAC 1 cut(s) 346
FatI CATG 8 cut(s) 39, 154, 181, 495, 594, 783, 850, 964
FauI CCCGC 1 cut(s) 524
Fnu4HI GCNGC 5 cut(s) 186, 257, 752, 926, 1120
FokI GGATG 3 cut(s) 19, 247, 735
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 5 cut(s) 186, 257, 752, 926, 1120
FspBI CTAG 5 cut(s) 90, 311, 603, 954, 1164
GluI GCNGC 5 cut(s) 186, 257, 752, 926, 1120
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 1 cut(s) 1073
HaeIII GGCC 3 cut(s) 79, 94, 1179
HapII CCGG 3 cut(s) 47, 293, 1176
HgaI GACGC 1 cut(s) 177
Hin1II CATG 8 cut(s) 43, 158, 185, 499, 598, 787, 854, 968
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 6 cut(s) 43, 50, 334, 358, 473, 521
HpaII CCGG 3 cut(s) 47, 293, 1176
HphI GGTGA 7 cut(s) 41, 203, 233, 280, 500, 749, 850
Hpy166II GTNNAC 3 cut(s) 152, 224, 578
Hpy188I TCNGA 4 cut(s) 69, 193, 505, 1107
Hpy188III TCNNGA 5 cut(s) 47, 276, 479, 851, 965
Hpy8I GTNNAC 3 cut(s) 152, 224, 578
Hpy99I CGWCG 3 cut(s) 330, 465, 485
HpyAV CCTTC 7 cut(s) 67, 378, 497, 511, 713, 768, 849
HpyCH4IV ACGT 2 cut(s) 411, 1066
HpyCH4V TGCA 6 cut(s) 578, 587, 594, 682, 751, 820
HpyF10VI GCNNNNNNNGC 4 cut(s) 361, 584, 817, 1099
HpyF3I CTNAG 5 cut(s) 199, 351, 375, 380, 971
HpySE526I ACGT 2 cut(s) 411, 1066
Hsp92II CATG 8 cut(s) 43, 158, 185, 499, 598, 787, 854, 968
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 5 cut(s) 505, 613, 967, 1072, 1129
LmnI GCTCC 3 cut(s) 163, 701, 991
Lsp1109I GCAGC 2 cut(s) 763, 937
LweI GCATC 2 cut(s) 807, 984
MaeI CTAG 5 cut(s) 90, 311, 603, 954, 1164
MaeII ACGT 2 cut(s) 411, 1066
MaeIII GTNAC 2 cut(s) 29, 1043
MalI GATC 5 cut(s) 507, 615, 969, 1074, 1131
MboI GATC 5 cut(s) 505, 613, 967, 1072, 1129
MboII GAAGA 6 cut(s) 572, 755, 839, 1061, 1064, 1067
MhlI GDGCHC 3 cut(s) 275, 310, 580
MluCI AATT 7 cut(s) 177, 555, 571, 731, 746, 778, 1183
MlyI GAGTC 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 47
MroI TCCGGA 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 413, 778
MseI TTAA 2 cut(s) 933, 1011
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 3 cut(s) 47, 293, 1176
MspR9I CCNGG 1 cut(s) 805
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 1 cut(s) 805
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 4 cut(s) 361, 584, 817, 1099
NdeII GATC 5 cut(s) 505, 613, 967, 1072, 1129
NlaIII CATG 8 cut(s) 43, 158, 185, 499, 598, 787, 854, 968
NlaIV GGNNCC 5 cut(s) 255, 284, 425, 728, 1174
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 274
PagI TCATGA 2 cut(s) 850, 964
PceI AGGCCT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 2 cut(s) 413, 778
PfeI GAWTC 5 cut(s) 43, 50, 358, 473, 521
PkrI GCNGC 5 cut(s) 187, 258, 753, 927, 1121
PleI GAGTC 1 cut(s) 328
PmaCI CACGTG 1 cut(s) 1067
PmlI CACGTG 1 cut(s) 1067
PpsI GAGTC 1 cut(s) 328
Ppu21I YACGTR 1 cut(s) 1067
PshBI ATTAAT 1 cut(s) 933
PsiI TTATAA 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 803
PspCI CACGTG 1 cut(s) 1067
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 1 cut(s) 803
PspN4I GGNNCC 5 cut(s) 255, 284, 425, 728, 1174
PspPI GGNCC 3 cut(s) 727, 916, 1178
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 3 cut(s) 151, 886, 1114
RsaNI GTAC 3 cut(s) 150, 885, 1113
SacI GAGCTC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 933, 1011
SatI GCNGC 5 cut(s) 186, 257, 752, 926, 1120
Sau3AI GATC 5 cut(s) 505, 613, 967, 1072, 1129
Sau96I GGNCC 3 cut(s) 727, 916, 1178
ScaI AGTACT 1 cut(s) 1114
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 1 cut(s) 805
SduI GDGCHC 3 cut(s) 275, 310, 580
SfaNI GCATC 2 cut(s) 807, 984
SfcI CTRYAG 2 cut(s) 789, 991
Sfr274I CTCGAG 1 cut(s) 274
SinI GGWCC 2 cut(s) 727, 916
SlaI CTCGAG 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SpeI ACTAGT 2 cut(s) 602, 953
Sse9I AATT 7 cut(s) 177, 555, 571, 731, 746, 778, 1183
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 5 cut(s) 186, 257, 531, 1100, 1119
SspMI CTAG 5 cut(s) 90, 311, 603, 954, 1164
SstI GAGCTC 1 cut(s) 275
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 1 cut(s) 803
StyI CCWWGG 1 cut(s) 89
TaiI ACGT 2 cut(s) 414, 1069
TaqI TCGA 4 cut(s) 175, 275, 325, 337
TasI AATT 7 cut(s) 177, 555, 571, 731, 746, 778, 1183
TatI WGTACW 2 cut(s) 149, 1112
TauI GCSGC 3 cut(s) 188, 259, 1122
TfiI GAWTC 5 cut(s) 43, 50, 358, 473, 521
Tru1I TTAA 2 cut(s) 933, 1011
Tru9I TTAA 2 cut(s) 933, 1011
TscAI CASTG 2 cut(s) 376, 552
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 2 cut(s) 751, 925
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 4 cut(s) 170, 567, 611, 839
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 2 cut(s) 376, 552
VneI GTGCAC 1 cut(s) 576
VpaK11BI GGWCC 2 cut(s) 727, 916
VspI ATTAAT 1 cut(s) 933
XapI RAATTY 2 cut(s) 177, 778
XceI RCATGY 1 cut(s) 158
XhoI CTCGAG 1 cut(s) 274
XmaJI CCTAGG 1 cut(s) 89
XmnI GAANNNNTTC 2 cut(s) 413, 778
XspI CTAG 5 cut(s) 90, 311, 603, 954, 1164
ZrmI AGTACT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.