MD06G1236500.v1.1
MYB Family

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008575
Physical Location & Seq
Forward (+)
36790086 .. 36793038
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1236500.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGGCAGTTGAGGATAAGATTGAAGCCACAGGGTCCAACGCCTCTGTTGCGGTTGGAAATTGCAGTTCAAATGGTGATGCGCAATCTAATGCGGAGATTTGTTCATTTGGCAGTGACCATGCACCCAAGGTTAGGAAACCGTATACGATTACAAAACAAAGAGCAAAATGGACAGAGGAGGAGCATCAGAAGTTCCTGGAAGCCTTGAAATTGTACGGCCGAGGTTGGCGTCAAATAGAAGAACATGTAGGCACCAAAACTGCAGTTCAAATTCGAAGTCATGCTCAAAAGTTCTTCTCTAAGGTATCTAAGGAGTCATGTGGACCTAGCGAAGGCTCCATCAGGCCTATTGAGATTCCTCCTCCGAGGCCTAAGCGGAAGCCTGTTCATCCTTATCCACGAAAATCAGTGGATTGTCTCAATGGAACACCAGAAAGGTCACCGTCCCCACAATTTTCGGCTCAAGGGAAAGATCAACAATCTCCTCCTTCAGTATTATCTGCACAGGGTTCAGATTTACTGGGGTCTGCAGCTTTGGACCAGCATAACAGATCTTCAACCCCGACTTCATGTACCACTGACATGCACTCAACGGGCCCATCCCATTTTGAAAAGGAGAATGATTCTATGACGTTCAGCTCATCTGCTGAAGAAGTGCAAGGGTCCTCGCCATCAGTCCAGTTATCTGCTGACTCAGTCTTGGAAAAATTTCCGTCCGTGAATTACAGATCAGGTTCCAAGCACACTGTGAGTACTGAAGAAGATGCTGGCAATTCAGGAGCTTCTACGAGCATTAAGCTCTTTGGAAGGACAGTTTCGGTACTAGACTCGGAGAAACAATCTCCAACTGGTGCCAAGGATTCTAAGACGCCGACATCAAAGGTGGAACAAGACAATTGTGAGAGGGAGAATGATGAGCTTGTTCAAACACCAAGATCAGATCTGTTAGATACGAGTTTGACACTTGGTGGGATTGTTGGTAATTTGTATCCATCGGCATGTGGTGGTACAACTACTTGGGAACACCAAAAACATAGCCCTAATCCTGAAGCTCCTCTGCCATGGTGGGATATGTATCATGGTCTACCGTGCTTCCACATCAGATCGTGTAACCAAAACTCAGCTCAAATACAAATACGTGTAGATTCTTGTGCGGACGAAACAATTAAACAGACGGAAAGATCATTTGGTGGTGCAGTGGAAAGTTTAGGAGAGAAGCACGTAGAGTCTGTCAGTTCGTTACGCGAAGAAACTCGTTCGGCTCCCTGCAGTTCACGGAAGGGGTTTGTGCCGTACAAAAGATGTTTAGCAGATGTAAATAAGAACTCATCTGCCATAGTTTCTGAAAACATGAGGGACAGGCGACGGGCTCGCGTCTGCTCATAG

Protein Analysis

462

Amino Acids

50.26

Weight (kDa)

6.92

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 55 - 97 1.8e-13 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 81
AccB1I GGYRCC 2 cut(s) 251, 851
AccI GTMKAC 2 cut(s) 143, 1084
AccII CGCG 2 cut(s) 1245, 1374
AciI CCGC 4 cut(s) 50, 92, 376, 1154
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 2 cut(s) 270, 707
AcuI CTGAAG 4 cut(s) 474, 669, 777, 1068
AcyI GRCGYC 2 cut(s) 229, 869
AdeI CACNNNGTG 2 cut(s) 748, 968
AfaI GTAC 6 cut(s) 215, 574, 754, 822, 1009, 1295
AfiI CCNNNNNNNGG 2 cut(s) 132, 332
AflIII ACRYGT 2 cut(s) 244, 1138
AgsI TTSAA 7 cut(s) 23, 69, 208, 269, 558, 611, 926
AjnI CCWGG 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AluBI AGCT 7 cut(s) 533, 639, 782, 799, 919, 1052, 1124
AluI AGCT 7 cut(s) 533, 639, 782, 799, 919, 1052, 1124
Alw26I GTCTC 1 cut(s) 422
AoxI GGCC 4 cut(s) 217, 344, 368, 595
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 1 cut(s) 530
ApoI RAATTY 2 cut(s) 270, 707
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 6 cut(s) 33, 323, 538, 595, 596, 663
AsuHPI GGTGA 2 cut(s) 86, 432
AsuII TTCGAA 1 cut(s) 274
AvaII GGWCC 4 cut(s) 33, 323, 538, 663
BaeGI GKGCMC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 251, 851
BanII GRGCYC 2 cut(s) 599, 1372
BbvI GCAGC 1 cut(s) 542
BccI CCATC 4 cut(s) 347, 607, 679, 1000
BceAI ACGGC 2 cut(s) 232, 1276
BciT130I CCWGG 1 cut(s) 197
BciVI GTATCC 1 cut(s) 999
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 327, 824
BfmI CTRYAG 3 cut(s) 261, 528, 1267
BfuI GTATCC 1 cut(s) 999
BglII AGATCT 2 cut(s) 551, 940
BisI GCNGC 1 cut(s) 531
BlsI GCNGC 1 cut(s) 532
BmcAI AGTACT 1 cut(s) 754
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 4 cut(s) 33, 323, 538, 663
BmgT120I GGNCC 6 cut(s) 33, 323, 538, 595, 596, 663
BmiI GGNNCC 8 cut(s) 34, 253, 337, 597, 664, 736, 853, 1263
BmrFI CCNGG 1 cut(s) 197
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 3 cut(s) 67, 193, 754
BmuI ACTGGG 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 447
BsaAI YACGTR 2 cut(s) 1139, 1222
BsaBI GATNNNNATC 1 cut(s) 1074
BsaHI GRCGYC 2 cut(s) 229, 869
BsaJI CCNNGG 5 cut(s) 126, 220, 365, 855, 1061
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 332
Bse1I ACTGG 3 cut(s) 525, 679, 853
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 5 cut(s) 126, 220, 365, 855, 1061
BseGI GGATG 2 cut(s) 388, 599
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 2 cut(s) 132, 332
BseMII CTCAG 2 cut(s) 708, 1134
BseNI ACTGG 3 cut(s) 525, 679, 853
BseRI GAGGAG 5 cut(s) 191, 194, 351, 474, 1044
BseSI GKGCMC 1 cut(s) 599
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 542
BsgI GTGCAG 2 cut(s) 486, 1215
Bsh1236I CGCG 2 cut(s) 1245, 1374
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 219, 346, 370, 597
BshNI GGYRCC 2 cut(s) 251, 851
BsiEI CGRYCG 1 cut(s) 220
BslFI GGGAC 2 cut(s) 430, 1370
BslI CCNNNNNNNGG 2 cut(s) 132, 332
BsmAI GTCTC 1 cut(s) 422
BsmFI GGGAC 2 cut(s) 430, 1370
BsnI GGCC 4 cut(s) 219, 346, 370, 597
Bsp119I TTCGAA 1 cut(s) 274
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 2 cut(s) 599, 1372
Bsp143I GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
Bsp19I CCATGG 1 cut(s) 1061
BspACI CCGC 4 cut(s) 50, 92, 376, 1154
BspANI GGCC 4 cut(s) 219, 346, 370, 597
BspCNI CTCAG 2 cut(s) 707, 1133
BspFNI CGCG 2 cut(s) 1245, 1374
BspLI GGNNCC 8 cut(s) 34, 253, 337, 597, 664, 736, 853, 1263
BspMAI CTGCAG 3 cut(s) 265, 532, 1271
BspT104I TTCGAA 1 cut(s) 274
BspT107I GGYRCC 2 cut(s) 251, 851
BsrI ACTGG 3 cut(s) 525, 679, 853
BssECI CCNNGG 5 cut(s) 126, 220, 365, 855, 1061
BssMI GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
BssNAI GTATAC 1 cut(s) 144
BssNI GRCGYC 2 cut(s) 229, 869
BssT1I CCWWGG 3 cut(s) 126, 855, 1061
Bst1107I GTATAC 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 5 cut(s) 141, 444, 748, 814, 1089
BstACI GRCGYC 2 cut(s) 229, 869
BstBAI YACGTR 2 cut(s) 1139, 1222
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 2 cut(s) 769, 1372
BstDEI CTNAG 6 cut(s) 300, 309, 372, 694, 864, 1120
BstDSI CCRYGG 1 cut(s) 1061
BstEII GGTNACC 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 388, 599
BstFNI CGCG 2 cut(s) 1245, 1374
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 7 cut(s) 475, 554, 731, 938, 943, 1106, 1184
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 3 cut(s) 248, 586, 1002
BstPI GGTNACC 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 3 cut(s) 261, 528, 1267
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 2 cut(s) 1245, 1374
BstV1I GCAGC 1 cut(s) 542
BstX2I RGATCY 2 cut(s) 551, 940
BstYI RGATCY 2 cut(s) 551, 940
BstZ17I GTATAC 1 cut(s) 144
BstZI CGGCCG 1 cut(s) 217
BsuI GTATCC 1 cut(s) 999
BsuRI GGCC 4 cut(s) 219, 346, 370, 597
BtgI CCRYGG 1 cut(s) 1061
BtsCI GGATG 2 cut(s) 388, 599
BtsI GCAGTG 2 cut(s) 118, 1203
BtsIMutI CAGTG 5 cut(s) 118, 414, 576, 744, 1203
Cac8I GCNNGC 2 cut(s) 769, 1372
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 6 cut(s) 33, 323, 538, 595, 596, 663
CseI GACGC 3 cut(s) 218, 877, 1363
Csp6I GTAC 6 cut(s) 214, 573, 753, 821, 1008, 1294
CviQI GTAC 6 cut(s) 214, 573, 753, 821, 1008, 1294
DdeI CTNAG 6 cut(s) 300, 309, 372, 694, 864, 1120
DpnI GATC 7 cut(s) 474, 553, 730, 937, 942, 1105, 1183
DpnII GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
DraIII CACNNNGTG 2 cut(s) 748, 968
EaeI YGGCCR 1 cut(s) 217
EagI CGGCCG 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 126, 855, 1061
Eco147I AGGCCT 2 cut(s) 346, 370
Eco24I GRGCYC 2 cut(s) 599, 1372
Eco47I GGWCC 4 cut(s) 33, 323, 538, 663
Eco52I CGGCCG 1 cut(s) 217
Eco57I CTGAAG 4 cut(s) 474, 669, 777, 1068
Eco91I GGTNACC 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 663
EcoO65I GGTNACC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 3 cut(s) 126, 855, 1061
EcoT38I GRGCYC 2 cut(s) 599, 1372
ErhI CCWWGG 3 cut(s) 126, 855, 1061
FaqI GGGAC 2 cut(s) 430, 1370
FblI GTMKAC 2 cut(s) 143, 1084
Fnu4HI GCNGC 1 cut(s) 531
FokI GGATG 2 cut(s) 375, 586
FriOI GRGCYC 2 cut(s) 599, 1372
Fsp4HI GCNGC 1 cut(s) 531
FspBI CTAG 2 cut(s) 327, 824
FspI TGCGCA 1 cut(s) 81
GlaI GCGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 531
HaeIII GGCC 4 cut(s) 219, 346, 370, 597
HgaI GACGC 3 cut(s) 218, 877, 1363
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 2 cut(s) 229, 869
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 8 cut(s) 314, 355, 623, 692, 827, 860, 1145, 1226
HphI GGTGA 2 cut(s) 86, 432
Hpy166II GTNNAC 4 cut(s) 144, 323, 1085, 1274
Hpy188I TCNGA 7 cut(s) 189, 366, 514, 832, 940, 1103, 1345
Hpy188III TCNNGA 2 cut(s) 777, 1046
Hpy8I GTNNAC 4 cut(s) 144, 323, 1085, 1274
Hpy99I CGWCG 1 cut(s) 1368
HpyAV CCTTC 4 cut(s) 326, 498, 801, 1273
HpyCH4III ACNGT 5 cut(s) 141, 444, 748, 814, 1089
HpyCH4IV ACGT 3 cut(s) 632, 1138, 1221
HpyCH4V TGCA 9 cut(s) 63, 122, 263, 503, 530, 586, 658, 1196, 1269
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 6 cut(s) 300, 309, 372, 694, 864, 1120
HpySE526I ACGT 3 cut(s) 632, 1138, 1221
Hsp92I GRCGYC 2 cut(s) 229, 869
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
LmnI GCTCC 5 cut(s) 181, 341, 779, 1057, 1267
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 3 cut(s) 67, 193, 754
MaeI CTAG 2 cut(s) 327, 824
MaeII ACGT 3 cut(s) 632, 1138, 1221
MaeIII GTNAC 4 cut(s) 113, 438, 1109, 1239
MalI GATC 7 cut(s) 474, 553, 730, 937, 942, 1105, 1183
MboI GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
MboII GAAGA 7 cut(s) 251, 286, 546, 662, 770, 773, 1259
MfeI CAATTG 1 cut(s) 895
MflI RGATCY 2 cut(s) 551, 940
MhlI GDGCHC 2 cut(s) 599, 1372
MlyI GAGTC 4 cut(s) 323, 686, 821, 1235
MmeI TCCRAC 3 cut(s) 34, 60, 869
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 2 cut(s) 795, 1167
MslI CAYNNNNRTG 2 cut(s) 581, 997
MspR9I CCNGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 895
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 2 cut(s) 1245, 1374
MwoI GCNNNNNNNGC 1 cut(s) 47
NcoI CCATGG 1 cut(s) 1061
NdeII GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
NlaIV GGNNCC 8 cut(s) 34, 253, 337, 597, 664, 736, 853, 1263
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 2 cut(s) 113, 438
NsbI TGCGCA 1 cut(s) 81
NspI RCATGY 3 cut(s) 248, 586, 1002
NspV TTCGAA 1 cut(s) 274
PceI AGGCCT 2 cut(s) 346, 370
PciI ACATGT 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 4 cut(s) 355, 623, 860, 1145
PflFI GACNNNGTC 1 cut(s) 695
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 1 cut(s) 532
PleI GAGTC 4 cut(s) 322, 686, 821, 1234
PpsI GAGTC 4 cut(s) 322, 686, 821, 1234
Ppu21I YACGTR 2 cut(s) 1139, 1222
PpuMI RGGWCCY 1 cut(s) 663
PscI ACATGT 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 195
PspEI GGTNACC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 8 cut(s) 34, 253, 337, 597, 664, 736, 853, 1263
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 6 cut(s) 33, 323, 538, 595, 596, 663
PspPPI RGGWCCY 1 cut(s) 663
PstI CTGCAG 3 cut(s) 265, 532, 1271
PsuI RGATCY 2 cut(s) 551, 940
PsyI GACNNNGTC 1 cut(s) 695
RsaI GTAC 6 cut(s) 215, 574, 754, 822, 1009, 1295
RsaNI GTAC 6 cut(s) 214, 573, 753, 821, 1008, 1294
RseI CAYNNNNRTG 2 cut(s) 581, 997
SaqAI TTAA 2 cut(s) 795, 1167
SatI GCNGC 1 cut(s) 531
Sau3AI GATC 7 cut(s) 472, 551, 728, 935, 940, 1103, 1181
Sau96I GGNCC 6 cut(s) 33, 323, 538, 595, 596, 663
ScaI AGTACT 1 cut(s) 754
SchI GAGTC 4 cut(s) 323, 686, 821, 1235
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 2 cut(s) 599, 1372
SfaNI GCATC 3 cut(s) 67, 193, 754
SfcI CTRYAG 3 cut(s) 261, 528, 1267
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 4 cut(s) 33, 323, 538, 663
SmiMI CAYNNNNRTG 2 cut(s) 581, 997
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
SseBI AGGCCT 2 cut(s) 346, 370
SsiI CCGC 4 cut(s) 50, 92, 376, 1154
SspMI CTAG 2 cut(s) 327, 824
StuI AGGCCT 2 cut(s) 346, 370
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 3 cut(s) 126, 855, 1061
TaaI ACNGT 5 cut(s) 141, 444, 748, 814, 1089
TaiI ACGT 3 cut(s) 635, 1141, 1224
TaqI TCGA 1 cut(s) 274
TatI WGTACW 1 cut(s) 752
TfiI GAWTC 4 cut(s) 355, 623, 860, 1145
Tru1I TTAA 2 cut(s) 795, 1167
Tru9I TTAA 2 cut(s) 795, 1167
TscAI CASTG 5 cut(s) 118, 414, 583, 751, 1203
TseFI GTSAC 2 cut(s) 113, 438
TseI GCWGC 1 cut(s) 530
Tsp45I GTSAC 2 cut(s) 113, 438
TspDTI ATGAA 3 cut(s) 93, 377, 558
TspGWI ACGGA 4 cut(s) 702, 706, 1190, 1291
TspRI CASTG 5 cut(s) 118, 414, 583, 751, 1203
Tth111I GACNNNGTC 1 cut(s) 695
VpaK11BI GGWCC 4 cut(s) 33, 323, 538, 663
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 2 cut(s) 270, 707
XceI RCATGY 3 cut(s) 248, 586, 1002
XmiI GTMKAC 2 cut(s) 143, 1084
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 2 cut(s) 327, 824
ZrmI AGTACT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.