MD14G1243400.v1.1
MYB Family

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008575
Physical Location & Seq
Forward (+)
32211589 .. 32214629
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1243400.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGCAGTTGAGGATCAGATTGAAGCCGCAGGTTCAAACGCCTCTGTTGCGGTTGGAAATTGCAGTTCAAACGGTGATGCGCAATCTAATGCGGAGATTTGTTCATTTGGCAATGACCATGCACCCAAGGTTAGGAAACCGTATACGATTACAAAACAAAGAGAAAAATGGACAGAGGAGGAGCATCAGAAGTTCCTTGAAGCCTTGAAATTGTACGGCCGAGGTTGGCGTCAAATAGAAGAATATGTAGGCACCAAAACTGCAGTTCAAATTCGAAGTCATGCTCAAAAGTTCTTCTCTAAGGTATCTAAGGAATCATGTGGTCCTAGCGAAGGCTCCACCAGACTGATTGAGATTCCTCCTCCCCGGCCTAAGCGGAAGCCTGTGCATCCTTATCCACGTAAATCAGTGGATTATCTCAATGGATCACCAGAAAGGTCGCCGTCCCCACAATTTTCAGCTCAAGGGAAAGACCAACAATCTCCCACTTCAGTACTATCTGCATATGATTCAGATGTACTGGGGTCTGCAGCTTTCGACCAGCATAACATATCTCCACCCCCGACTTCATGTACCACTGACATGCACTCTACAAGCTTATCCCCCTTTGGAAAAGAGAGTGATTACATGGCGTCCAACTCATCCGCTGATGAAGTGAAAGGGTCCTCGCCATCGGTCCATTTGGAAAAATTTCCATCCGTGAATTACAGACCAGGTTCCAAGCACACTGTGAATACTGAAGATGATGCCGGCAATATAAGAGCTTCTGTGAGCATTAAGCTCTTTGGAAGGACAGTTTTGGTACCAGACTCGGAGAAACAATCTCCACCTGGTGCTGACGAGTCTAAGACATTGACGTCAAAGGTGGAACAAGACAACTGTGAGAGGGAGAATGATAAGCTTGTTCAAATATCAAGATCAGATCAATTAGATACGAGTTTAACACTTGGTGGGATTGTTGGCAACGTGGTTTCATCGGCATGTGTTGCTACAACAAAATGGGAACACCAAAAACATAGCCCTAATCCTGAAGCTCCTCTGCCATGGTGGGCTATGCATCATGGTTTCCCGTACCGTCACGTCAGATCATGTAACGAAAACTCATCTAAAATTACGGAAAGATCCTTTCATAGTGCAGTGGAAAGTTTAGGTGAGAGCCACATAGAGGCTGTCGATTCATTACGCGAAGAAACTTGTTCGGAGCCCTGCAGTTCACGGAAGGGGTTTGCAGCGTACAAAAGATGTTTAGCAGATGTAAATAAGAACTCATCTGCCATGGTTTATGAAGACCTGAGGGACAGGCGAAGGGCTCGTGTCTGCTCATAG

Protein Analysis

442

Amino Acids

48.56

Weight (kDa)

6.74

Isoelectric Point (pI)

65.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 54 - 97 2.6e-13 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 856
AatII GACGTC 1 cut(s) 860
Acc16I TGCGCA 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 20
Acc65I GGTACC 1 cut(s) 802
AccB1I GGYRCC 2 cut(s) 251, 802
AccI GTMKAC 1 cut(s) 143
AccII CGCG 1 cut(s) 1185
AciI CCGC 5 cut(s) 27, 50, 92, 376, 645
AclWI GGATC 3 cut(s) 21, 433, 1116
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 2 cut(s) 270, 689
AcuI CTGAAG 3 cut(s) 474, 759, 1050
AcyI GRCGYC 3 cut(s) 229, 632, 857
AdeI CACNNNGTG 3 cut(s) 730, 833, 950
AfaI GTAC 7 cut(s) 215, 495, 519, 574, 804, 1073, 1235
AfiI CCNNNNNNNGG 3 cut(s) 132, 332, 1165
AgsI TTSAA 7 cut(s) 23, 36, 69, 200, 208, 269, 908
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 2 cut(s) 712, 829
AluBI AGCT 7 cut(s) 461, 533, 597, 764, 781, 901, 1034
AluI AGCT 7 cut(s) 461, 533, 597, 764, 781, 901, 1034
AlwI GGATC 3 cut(s) 21, 433, 1116
AoxI GGCC 2 cut(s) 217, 368
ApeKI GCWGC 2 cut(s) 530, 1229
ApoI RAATTY 2 cut(s) 270, 689
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
Asp700I GAANNNNTTC 1 cut(s) 690
Asp718I GGTACC 1 cut(s) 802
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 3 cut(s) 323, 663, 676
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 3 cut(s) 86, 420, 1163
AsuII TTCGAA 1 cut(s) 274
AvaII GGWCC 3 cut(s) 323, 663, 676
AxyI CCTNAGG 1 cut(s) 1292
BanI GGYRCC 2 cut(s) 251, 802
BanII GRGCYC 2 cut(s) 1206, 1312
BauI CACGAG 1 cut(s) 1311
BbsI GAAGAC 1 cut(s) 1293
BbvI GCAGC 2 cut(s) 542, 1241
BccI CCATC 2 cut(s) 679, 703
BceAI ACGGC 2 cut(s) 232, 427
BciT130I CCWGG 2 cut(s) 714, 831
BcnI CCSGG 1 cut(s) 367
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 3 cut(s) 261, 528, 1207
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 3 cut(s) 27, 531, 1230
BlsI GCNGC 3 cut(s) 28, 532, 1231
BmcAI AGTACT 1 cut(s) 495
Bme1390I CCNGG 3 cut(s) 367, 714, 831
Bme18I GGWCC 3 cut(s) 323, 663, 676
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 3 cut(s) 323, 663, 676
BmiI GGNNCC 6 cut(s) 253, 337, 664, 718, 804, 1203
BmrFI CCNGG 3 cut(s) 367, 714, 831
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 5 cut(s) 67, 193, 397, 736, 1066
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 1293
Bpu10I CCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 401
BsaHI GRCGYC 3 cut(s) 229, 632, 857
BsaJI CCNNGG 5 cut(s) 126, 220, 365, 1043, 1275
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 332, 1165
Bse118I RCCGGY 1 cut(s) 749
Bse1I ACTGG 1 cut(s) 525
Bse21I CCTNAGG 1 cut(s) 1292
Bse3DI GCAATG 1 cut(s) 118
BseBI CCWGG 2 cut(s) 714, 831
BseDI CCNNGG 5 cut(s) 126, 220, 365, 1043, 1275
BseGI GGATG 3 cut(s) 388, 641, 695
BseLI CCNNNNNNNGG 3 cut(s) 132, 332, 1165
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 1283
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 4 cut(s) 191, 194, 351, 1026
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 542, 1241
BsgI GTGCAG 1 cut(s) 1155
Bsh1236I CGCG 1 cut(s) 1185
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 2 cut(s) 219, 370
BshNI GGYRCC 2 cut(s) 251, 802
BsiEI CGRYCG 1 cut(s) 220
BsiSI CCGG 2 cut(s) 367, 750
BslFI GGGAC 2 cut(s) 430, 1310
BslI CCNNNNNNNGG 3 cut(s) 132, 332, 1165
BsmFI GGGAC 2 cut(s) 430, 1310
BsnI GGCC 2 cut(s) 219, 370
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 1206, 1312
Bsp143I GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
Bsp19I CCATGG 2 cut(s) 1043, 1275
BspACI CCGC 5 cut(s) 27, 50, 92, 376, 645
BspANI GGCC 2 cut(s) 219, 370
BspCNI CTCAG 1 cut(s) 1284
BspFNI CGCG 1 cut(s) 1185
BspLI GGNNCC 6 cut(s) 253, 337, 664, 718, 804, 1203
BspMAI CTGCAG 3 cut(s) 265, 532, 1211
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 3 cut(s) 21, 433, 1116
BspT104I TTCGAA 1 cut(s) 274
BspT107I GGYRCC 2 cut(s) 251, 802
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 749
BsrI ACTGG 1 cut(s) 525
BssAI RCCGGY 1 cut(s) 749
BssECI CCNNGG 5 cut(s) 126, 220, 365, 1043, 1275
BssMI GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
BssNAI GTATAC 1 cut(s) 144
BssNI GRCGYC 3 cut(s) 229, 632, 857
BssSI CACGAG 1 cut(s) 1311
BssT1I CCWWGG 3 cut(s) 126, 1043, 1275
Bst1107I GTATAC 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 1311
Bst2UI CCWGG 2 cut(s) 714, 831
Bst4CI ACNGT 6 cut(s) 74, 141, 730, 796, 881, 1076
BstACI GRCGYC 3 cut(s) 229, 632, 857
BstAPI GCANNNNNTGC 1 cut(s) 986
BstBAI YACGTR 1 cut(s) 401
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 751
BstDEI CTNAG 5 cut(s) 300, 309, 372, 846, 1292
BstDSI CCRYGG 2 cut(s) 1043, 1275
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 388, 641, 695
BstFNI CGCG 1 cut(s) 1185
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 6 cut(s) 16, 428, 920, 925, 1088, 1124
BstMBI GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 2 cut(s) 47, 986
BstNI CCWGG 2 cut(s) 714, 831
BstNSI RCATGY 2 cut(s) 586, 984
BstSCI CCNGG 3 cut(s) 365, 712, 829
BstSFI CTRYAG 3 cut(s) 261, 528, 1207
BstUI CGCG 1 cut(s) 1185
BstV1I GCAGC 2 cut(s) 542, 1241
BstV2I GAAGAC 1 cut(s) 1293
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BstZ17I GTATAC 1 cut(s) 144
BstZI CGGCCG 1 cut(s) 217
Bsu36I CCTNAGG 1 cut(s) 1292
BsuRI GGCC 2 cut(s) 219, 370
BtgI CCRYGG 2 cut(s) 1043, 1275
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 3 cut(s) 388, 641, 695
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 4 cut(s) 414, 576, 726, 1143
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 751
CfoI GCGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 749
Cfr13I GGNCC 3 cut(s) 323, 663, 676
CseI GACGC 2 cut(s) 218, 621
CsiI ACCWGGT 2 cut(s) 712, 829
Csp6I GTAC 7 cut(s) 214, 494, 518, 573, 803, 1072, 1234
CviQI GTAC 7 cut(s) 214, 494, 518, 573, 803, 1072, 1234
DdeI CTNAG 5 cut(s) 300, 309, 372, 846, 1292
DpnI GATC 6 cut(s) 15, 427, 919, 924, 1087, 1123
DpnII GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
DraIII CACNNNGTG 3 cut(s) 730, 833, 950
DrdI GACNNNNNNGTC 1 cut(s) 856
DseDI GACNNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 217
EagI CGGCCG 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 126, 1043, 1275
Eco24I GRGCYC 2 cut(s) 1206, 1312
Eco47I GGWCC 3 cut(s) 323, 663, 676
Eco52I CGGCCG 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 474, 759, 1050
Eco81I CCTNAGG 1 cut(s) 1292
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 663
EcoRII CCWGG 2 cut(s) 712, 829
EcoT14I CCWWGG 3 cut(s) 126, 1043, 1275
EcoT22I ATGCAT 1 cut(s) 1059
EcoT38I GRGCYC 2 cut(s) 1206, 1312
ErhI CCWWGG 3 cut(s) 126, 1043, 1275
FaqI GGGAC 2 cut(s) 430, 1310
FauNDI CATATG 1 cut(s) 505
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 3 cut(s) 27, 531, 1230
FokI GGATG 3 cut(s) 375, 628, 682
FriOI GRGCYC 2 cut(s) 1206, 1312
Fsp4HI GCNGC 3 cut(s) 27, 531, 1230
FspBI CTAG 1 cut(s) 327
FspI TGCGCA 1 cut(s) 81
GlaI GCGC 1 cut(s) 81
GluI GCNGC 3 cut(s) 27, 531, 1230
HaeIII GGCC 2 cut(s) 219, 370
HapII CCGG 2 cut(s) 367, 750
HgaI GACGC 2 cut(s) 218, 621
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 3 cut(s) 229, 632, 857
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HindIII AAGCTT 2 cut(s) 595, 899
HinfI GANTC 6 cut(s) 314, 355, 509, 809, 842, 1175
HpaII CCGG 2 cut(s) 367, 750
HphI GGTGA 3 cut(s) 86, 420, 1163
Hpy166II GTNNAC 2 cut(s) 144, 1214
Hpy188I TCNGA 7 cut(s) 18, 189, 514, 814, 922, 1085, 1201
Hpy188III TCNNGA 2 cut(s) 915, 1028
Hpy8I GTNNAC 2 cut(s) 144, 1214
HpyAV CCTTC 4 cut(s) 326, 783, 1213, 1299
HpyCH4III ACNGT 6 cut(s) 74, 141, 730, 796, 881, 1076
HpyCH4IV ACGT 4 cut(s) 400, 857, 966, 1080
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 986
HpyF3I CTNAG 5 cut(s) 300, 309, 372, 846, 1292
HpySE526I ACGT 4 cut(s) 400, 857, 966, 1080
Hsp92I GRCGYC 3 cut(s) 229, 632, 857
HspAI GCGC 1 cut(s) 80
KpnI GGTACC 1 cut(s) 806
KroI GCCGGC 1 cut(s) 749
KroNI GCCGGC 1 cut(s) 751
Kzo9I GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
LmnI GCTCC 4 cut(s) 181, 341, 1039, 1201
Lsp1109I GCAGC 2 cut(s) 542, 1241
LweI GCATC 5 cut(s) 67, 193, 397, 736, 1066
MabI ACCWGGT 2 cut(s) 712, 829
MaeI CTAG 1 cut(s) 327
MaeII ACGT 4 cut(s) 400, 857, 966, 1080
MaeIII GTNAC 2 cut(s) 1076, 1091
MalI GATC 6 cut(s) 15, 427, 919, 924, 1087, 1123
MboI GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
MboII GAAGA 5 cut(s) 251, 286, 752, 1199, 1298
MflI RGATCY 1 cut(s) 1121
MhlI GDGCHC 2 cut(s) 1206, 1312
MluCI AATT 8 cut(s) 58, 209, 270, 452, 689, 703, 926, 1110
MlyI GAGTC 2 cut(s) 803, 851
MmeI TCCRAC 2 cut(s) 34, 660
Mph1103I ATGCAT 1 cut(s) 1059
MroNI GCCGGC 1 cut(s) 749
MroXI GAANNNNTTC 1 cut(s) 690
MseI TTAA 2 cut(s) 777, 941
MslI CAYNNNNRTG 2 cut(s) 581, 979
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 2 cut(s) 367, 750
MspR9I CCNGG 3 cut(s) 367, 714, 831
MvaI CCWGG 2 cut(s) 714, 831
MvnI CGCG 1 cut(s) 1185
MwoI GCNNNNNNNGC 2 cut(s) 47, 986
NaeI GCCGGC 1 cut(s) 751
NciI CCSGG 1 cut(s) 367
NcoI CCATGG 2 cut(s) 1043, 1275
NdeI CATATG 1 cut(s) 505
NdeII GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
NgoMIV GCCGGC 1 cut(s) 749
NlaIV GGNNCC 6 cut(s) 253, 337, 664, 718, 804, 1203
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 1076
NsbI TGCGCA 1 cut(s) 81
NsiI ATGCAT 1 cut(s) 1059
NspI RCATGY 2 cut(s) 586, 984
NspV TTCGAA 1 cut(s) 274
PdiI GCCGGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 690
PfeI GAWTC 4 cut(s) 314, 355, 509, 1175
PkrI GCNGC 3 cut(s) 28, 532, 1231
PleI GAGTC 2 cut(s) 803, 850
PpsI GAGTC 2 cut(s) 803, 850
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 712, 829
PspGI CCWGG 2 cut(s) 712, 829
PspN4I GGNNCC 6 cut(s) 253, 337, 664, 718, 804, 1203
PspPI GGNCC 3 cut(s) 323, 663, 676
PspPPI RGGWCCY 1 cut(s) 663
PstI CTGCAG 3 cut(s) 265, 532, 1211
PsuI RGATCY 1 cut(s) 1121
RsaI GTAC 7 cut(s) 215, 495, 519, 574, 804, 1073, 1235
RsaNI GTAC 7 cut(s) 214, 494, 518, 573, 803, 1072, 1234
RseI CAYNNNNRTG 2 cut(s) 581, 979
SaqAI TTAA 2 cut(s) 777, 941
SatI GCNGC 3 cut(s) 27, 531, 1230
Sau3AI GATC 6 cut(s) 13, 425, 917, 922, 1085, 1121
Sau96I GGNCC 3 cut(s) 323, 663, 676
ScaI AGTACT 1 cut(s) 495
SchI GAGTC 2 cut(s) 803, 851
ScrFI CCNGG 3 cut(s) 367, 714, 831
SduI GDGCHC 2 cut(s) 1206, 1312
SexAI ACCWGGT 2 cut(s) 712, 829
SfaNI GCATC 5 cut(s) 67, 193, 397, 736, 1066
SfcI CTRYAG 3 cut(s) 261, 528, 1207
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 3 cut(s) 323, 663, 676
SmiMI CAYNNNNRTG 2 cut(s) 581, 979
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 8 cut(s) 58, 209, 270, 452, 689, 703, 926, 1110
SsiI CCGC 5 cut(s) 27, 50, 92, 376, 645
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 3 cut(s) 365, 712, 829
StyI CCWWGG 3 cut(s) 126, 1043, 1275
TaaI ACNGT 6 cut(s) 74, 141, 730, 796, 881, 1076
TaiI ACGT 4 cut(s) 403, 860, 969, 1083
TaqI TCGA 3 cut(s) 274, 537, 1173
TaqII GACCGA 1 cut(s) 664
TasI AATT 8 cut(s) 58, 209, 270, 452, 689, 703, 926, 1110
TatI WGTACW 2 cut(s) 493, 517
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 4 cut(s) 314, 355, 509, 1175
Tru1I TTAA 2 cut(s) 777, 941
Tru9I TTAA 2 cut(s) 777, 941
TscAI CASTG 4 cut(s) 414, 583, 733, 1143
TseFI GTSAC 1 cut(s) 1076
TseI GCWGC 2 cut(s) 530, 1229
Tsp45I GTSAC 1 cut(s) 1076
TspDTI ATGAA 7 cut(s) 93, 558, 666, 963, 1118, 1167, 1299
TspGWI ACGGA 3 cut(s) 688, 1130, 1231
TspRI CASTG 4 cut(s) 414, 583, 733, 1143
VpaK11BI GGWCC 3 cut(s) 323, 663, 676
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 2 cut(s) 270, 689
XceI RCATGY 2 cut(s) 586, 984
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 690
XspI CTAG 1 cut(s) 327
ZraI GACGTC 1 cut(s) 858
ZrmI AGTACT 1 cut(s) 495
Zsp2I ATGCAT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.