MD07G1002600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007084
Physical Location & Seq
Reverse (-)
279701 .. 285566
5866 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1002600.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCCATGGATCCCAACCACACCACTGCTTCAGCCGCCACGGCCACCGCCGGAGCCGCCGCCCCCAACACACCCACGACGAGGCCCGTAAGACCCGCCGTATCATCTCCTCTTCCTCCTAATCTTTACGCAGCCCAATCCCTCCAAATTCGGACCCAATCGCCATCATCATCACCATTAGCATCAGCAGCTGCTAATCATCAGGGGGTTCTGTACCCACTCGCTTCCTCAGGCCGAGGCTTCATTACCCGGCCCATTCGCCCCGCCGCCGCTGCAGGAGGCGAACCCCCTGTCACTGTGGCCAACGCAGGAACTTCGTACCCATCTCGGCCCCATCTCAATTTCCCTCCGCAGCAGCCTATTTCTGTGCATTTGATGAGACCCAACTACAGCTTAGCACCTTCTCCTCTTCCTCCTCCTATTAGGGGCCTTCCCCTTTCTTCTGCTGCTCCTGAGGTTGCTCCATCTTCAGTTCCTGACAGCAATGGTTTTAAAGACATGAGGGATAAAAGCAAAGATGATAACTTAGCTGTCATTAGAGGTCGAAAAGTCAGGATGACAGATGGAGCTTCTCTATATGTGCACTGTCGGTCATGGATGAGGAACGGTTTTCCTGAAGAAATTCAGCCACAATATGGTGATGCTGTAAGGTCTCTTCCGAAACCTTCACCAATACCTATGGCAAGTGCTACTCTTCCAAAGAAGGAGGGCGGTGAAGCAGAAGAAAAAGGAGATGATAACAAGGACGAGGCTGAGGAACACATTGAGCGTTTATCACCACATGATCTGTTAAAAAGACATGTCAAACGCGCCAGAAAGGTTCGAGCACGATTAAGAGAAGAACGCTTACAGCGAATTGCAAGGTACAAGTCTAGGCTTGCGCTTCTCCTTCCTCCCTTGGTAGAACAGTTCAGAAATGATCTAGCTTCAGGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN37

Protein Analysis

313

Amino Acids

33.66

Weight (kDa)

10.07

Isoelectric Point (pI)

64.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIN37 PF15306 209 - 296 4.5e-06 LIN37
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 635
AccII CGCG 1 cut(s) 810
AclWI GGATC 2 cut(s) 5, 18
AcoI YGGCCR 3 cut(s) 3, 42, 300
AcsI RAATTY 2 cut(s) 147, 621
AcuI CTGAAG 4 cut(s) 15, 453, 636, 912
AfaI GTAC 3 cut(s) 215, 320, 866
AfiI CCNNNNNNNGG 3 cut(s) 81, 425, 635
AflIII ACRYGT 1 cut(s) 799
AluBI AGCT 5 cut(s) 191, 393, 530, 569, 926
AluI AGCT 5 cut(s) 191, 393, 530, 569, 926
Alw21I GWGCWC 2 cut(s) 585, 829
Alw26I GTCTC 2 cut(s) 373, 657
Alw44I GTGCAC 1 cut(s) 581
AlwI GGATC 2 cut(s) 5, 18
AlwNI CAGNNNCTG 2 cut(s) 191, 476
AoxI GGCC 8 cut(s) 3, 42, 83, 232, 251, 300, 329, 427
ApaLI GTGCAC 1 cut(s) 581
ApeKI GCWGC 7 cut(s) 131, 188, 191, 272, 352, 355, 446
ApoI RAATTY 2 cut(s) 147, 621
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 2 cut(s) 812, 883
AspS9I GGNCC 5 cut(s) 84, 153, 252, 330, 427
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 5 cut(s) 165, 650, 660, 725, 768
AvaII GGWCC 1 cut(s) 153
AxyI CCTNAGG 2 cut(s) 229, 453
BaeGI GKGCMC 1 cut(s) 585
BalI TGGCCA 2 cut(s) 5, 302
BamHI GGATCC 1 cut(s) 10
BarI GAAGNNNNNNTAC 4 cut(s) 639, 671, 831, 863
Bbv12I GWGCWC 2 cut(s) 585, 829
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 7 cut(s) 143, 178, 200, 259, 364, 367, 433
BccI CCATC 5 cut(s) 172, 331, 342, 472, 557
BceAI ACGGC 2 cut(s) 57, 83
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 373, 657
BfaI CTAG 2 cut(s) 873, 923
BfmI CTRYAG 2 cut(s) 273, 388
BglI GCCNNNNNGGC 1 cut(s) 41
BlpI GCTNAGC 1 cut(s) 394
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 153
BmgT120I GGNCC 5 cut(s) 84, 153, 252, 330, 427
BmiI GGNNCC 5 cut(s) 12, 55, 155, 332, 428
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 191, 631
Bpu10I CCTNAGC 1 cut(s) 753
Bpu1102I GCTNAGC 1 cut(s) 394
BpuMI CCSGG 1 cut(s) 250
BsaI GGTCTC 2 cut(s) 373, 657
BsaJI CCNNGG 4 cut(s) 6, 39, 235, 897
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 425, 635
Bse21I CCTNAGG 2 cut(s) 229, 453
Bse3DI GCAATG 1 cut(s) 490
BseDI CCNNGG 4 cut(s) 6, 39, 235, 897
BseGI GGATG 2 cut(s) 561, 603
BseLI CCNNNNNNNGG 3 cut(s) 81, 425, 635
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 243, 444, 744
BseRI GAGGAG 3 cut(s) 99, 396, 405
BseSI GKGCMC 1 cut(s) 585
BseXI GCAGC 7 cut(s) 143, 178, 200, 259, 364, 367, 433
Bsh1236I CGCG 1 cut(s) 810
BshFI GGCC 8 cut(s) 5, 44, 85, 234, 253, 302, 331, 429
BsiHKAI GWGCWC 2 cut(s) 585, 829
BsiSI CCGG 2 cut(s) 51, 250
BslI CCNNNNNNNGG 3 cut(s) 81, 425, 635
BsmAI GTCTC 2 cut(s) 373, 657
BsnI GGCC 8 cut(s) 5, 44, 85, 234, 253, 302, 331, 429
Bso31I GGTCTC 2 cut(s) 373, 657
Bsp1286I GDGCHC 2 cut(s) 585, 829
Bsp143I GATC 3 cut(s) 10, 784, 919
Bsp1720I GCTNAGC 1 cut(s) 394
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 8 cut(s) 5, 44, 85, 234, 253, 302, 331, 429
BspCNI CTCAG 3 cut(s) 242, 445, 745
BspFNI CGCG 1 cut(s) 810
BspLI GGNNCC 5 cut(s) 12, 55, 155, 332, 428
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 2 cut(s) 5, 18
BspTNI GGTCTC 2 cut(s) 373, 657
BsrDI GCAATG 1 cut(s) 490
BssECI CCNNGG 4 cut(s) 6, 39, 235, 897
BssMI GATC 3 cut(s) 10, 784, 919
BssT1I CCWWGG 2 cut(s) 6, 897
Bst4CI ACNGT 4 cut(s) 298, 587, 608, 909
Bst6I CTCTTC 4 cut(s) 117, 414, 660, 699
BstC8I GCNNGC 1 cut(s) 879
BstDEI CTNAG 5 cut(s) 229, 394, 453, 526, 753
BstDSI CCRYGG 2 cut(s) 6, 39
BstF5I GGATG 2 cut(s) 561, 603
BstFNI CGCG 1 cut(s) 810
BstHHI GCGC 2 cut(s) 812, 883
BstKTI GATC 3 cut(s) 13, 787, 922
BstMAI GTCTC 2 cut(s) 373, 657
BstMBI GATC 3 cut(s) 10, 784, 919
BstMWI GCNNNNNNNGC 5 cut(s) 35, 41, 56, 188, 272
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 1 cut(s) 248
BstSFI CTRYAG 2 cut(s) 273, 388
BstSLI GKGCMC 1 cut(s) 585
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 7 cut(s) 143, 178, 200, 259, 364, 367, 433
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
Bsu36I CCTNAGG 2 cut(s) 229, 453
BsuRI GGCC 8 cut(s) 5, 44, 85, 234, 253, 302, 331, 429
BtgI CCRYGG 2 cut(s) 6, 39
BtsCI GGATG 2 cut(s) 561, 603
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 3 cut(s) 24, 294, 583
Cac8I GCNNGC 1 cut(s) 879
CaiI CAGNNNCTG 2 cut(s) 191, 476
CfoI GCGC 2 cut(s) 812, 883
Cfr13I GGNCC 5 cut(s) 84, 153, 252, 330, 427
Csp6I GTAC 3 cut(s) 214, 319, 865
CviAII CATG 5 cut(s) 7, 499, 594, 782, 800
CviQI GTAC 3 cut(s) 214, 319, 865
DdeI CTNAG 5 cut(s) 229, 394, 453, 526, 753
DpnI GATC 3 cut(s) 12, 786, 921
DpnII GATC 3 cut(s) 10, 784, 919
DraI TTTAAA 1 cut(s) 493
EaeI YGGCCR 3 cut(s) 3, 42, 300
Eam1104I CTCTTC 4 cut(s) 117, 414, 660, 699
EarI CTCTTC 4 cut(s) 117, 414, 660, 699
Eco130I CCWWGG 2 cut(s) 6, 897
Eco31I GGTCTC 2 cut(s) 373, 657
Eco47I GGWCC 1 cut(s) 153
Eco57I CTGAAG 4 cut(s) 15, 453, 636, 912
Eco81I CCTNAGG 2 cut(s) 229, 453
EcoO109I RGGNCCY 1 cut(s) 427
EcoT14I CCWWGG 2 cut(s) 6, 897
ErhI CCWWGG 2 cut(s) 6, 897
FaeI CATG 5 cut(s) 10, 502, 597, 785, 803
FaiI YATR 9 cut(s) 8, 500, 577, 579, 595, 636, 680, 783, 801
FatI CATG 5 cut(s) 6, 498, 593, 781, 799
FauI CCCGC 2 cut(s) 103, 271
FokI GGATG 2 cut(s) 568, 610
FspBI CTAG 2 cut(s) 873, 923
GlaI GCGC 2 cut(s) 811, 882
HaeIII GGCC 8 cut(s) 5, 44, 85, 234, 253, 302, 331, 429
HapII CCGG 2 cut(s) 51, 250
HhaI GCGC 2 cut(s) 812, 883
Hin1II CATG 5 cut(s) 10, 502, 597, 785, 803
Hin6I GCGC 2 cut(s) 810, 881
HinP1I GCGC 2 cut(s) 810, 881
HpaII CCGG 2 cut(s) 51, 250
HphI GGTGA 5 cut(s) 165, 650, 660, 725, 768
Hpy166II GTNNAC 1 cut(s) 583
Hpy188I TCNGA 3 cut(s) 153, 660, 914
Hpy188III TCNNGA 5 cut(s) 452, 476, 553, 614, 930
Hpy8I GTNNAC 1 cut(s) 583
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 5 cut(s) 411, 440, 675, 697, 899
HpyCH4III ACNGT 4 cut(s) 298, 587, 608, 909
HpyCH4V TGCA 4 cut(s) 275, 370, 583, 860
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 41, 56, 188, 272
HpyF3I CTNAG 5 cut(s) 229, 394, 453, 526, 753
Hsp92II CATG 5 cut(s) 10, 502, 597, 785, 803
HspAI GCGC 2 cut(s) 810, 881
Kzo9I GATC 3 cut(s) 10, 784, 919
LmnI GCTCC 4 cut(s) 53, 454, 466, 566
Lsp1109I GCAGC 7 cut(s) 143, 178, 200, 259, 364, 367, 433
LweI GCATC 2 cut(s) 191, 631
MaeI CTAG 2 cut(s) 873, 923
MaeIII GTNAC 1 cut(s) 292
MalI GATC 3 cut(s) 12, 786, 921
MboI GATC 3 cut(s) 10, 784, 919
MboII GAAGA 9 cut(s) 104, 401, 432, 459, 629, 647, 686, 734, 851
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 2 cut(s) 585, 829
MlsI TGGCCA 2 cut(s) 5, 302
MluCI AATT 4 cut(s) 147, 340, 621, 855
MluNI TGGCCA 2 cut(s) 5, 302
Mox20I TGGCCA 2 cut(s) 5, 302
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 2 cut(s) 5, 302
MseI TTAA 3 cut(s) 492, 791, 833
Msp20I TGGCCA 2 cut(s) 5, 302
MspA1I CMGCKG 2 cut(s) 191, 272
MspI CCGG 2 cut(s) 51, 250
MspR9I CCNGG 1 cut(s) 250
MvnI CGCG 1 cut(s) 810
MwoI GCNNNNNNNGC 5 cut(s) 35, 41, 56, 188, 272
NciI CCSGG 1 cut(s) 250
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 10, 784, 919
NlaIII CATG 5 cut(s) 10, 502, 597, 785, 803
NlaIV GGNNCC 5 cut(s) 12, 55, 155, 332, 428
NmeAIII GCCGAG 2 cut(s) 260, 307
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 1 cut(s) 803
PciI ACATGT 1 cut(s) 799
PcsI WCGNNNNNNNCGW 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 621
PflMI CCANNNNNTGG 1 cut(s) 635
PscI ACATGT 1 cut(s) 799
PspN4I GGNNCC 5 cut(s) 12, 55, 155, 332, 428
PspPI GGNCC 5 cut(s) 84, 153, 252, 330, 427
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 2 cut(s) 191, 476
PsuI RGATCY 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 3 cut(s) 215, 320, 866
RsaNI GTAC 3 cut(s) 214, 319, 865
SaqAI TTAA 3 cut(s) 492, 791, 833
Sau3AI GATC 3 cut(s) 10, 784, 919
Sau96I GGNCC 5 cut(s) 84, 153, 252, 330, 427
ScrFI CCNGG 1 cut(s) 250
SduI GDGCHC 2 cut(s) 585, 829
SfaNI GCATC 2 cut(s) 191, 631
SfcI CTRYAG 2 cut(s) 273, 388
SinI GGWCC 1 cut(s) 153
Sse9I AATT 4 cut(s) 147, 340, 621, 855
SspMI CTAG 2 cut(s) 873, 923
StyD4I CCNGG 1 cut(s) 248
StyI CCWWGG 2 cut(s) 6, 897
TaaI ACNGT 4 cut(s) 298, 587, 608, 909
TaqI TCGA 2 cut(s) 544, 823
TaqII GACCGA 1 cut(s) 579
TasI AATT 4 cut(s) 147, 340, 621, 855
TauI GCSGC 5 cut(s) 38, 59, 62, 269, 272
Tru1I TTAA 3 cut(s) 492, 791, 833
Tru9I TTAA 3 cut(s) 492, 791, 833
TscAI CASTG 3 cut(s) 31, 301, 590
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 7 cut(s) 131, 188, 191, 272, 352, 355, 446
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 1 cut(s) 232
TspRI CASTG 3 cut(s) 31, 301, 590
Van91I CCANNNNNTGG 1 cut(s) 635
VneI GTGCAC 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 153
XapI RAATTY 2 cut(s) 147, 621
XceI RCATGY 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 2 cut(s) 873, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.