RorugPtG0004800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000008
OG0007084
Physical Location & Seq
Forward (+)
115932 .. 120208
4277 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugPtG0004800.1

Sequence Viewer

Length: 1182 bp
ATGAATATACCAGCTCCAAGAAAAGACCTCATGGTAGTCAATATGGGTCCCCACCATCCATCAATGCATGGTGTTCTTCGACTTATCATTACTCTAGATGGTGAAGATGTTATTGACTGTGAACCAATATTGGGTTATTTACACCGAGGGATGGAAAAAATTGCGGAAAACCGAACAATTATACAATATTTGCCTTATGTAACACGTTGGGATTATTTAGCTACTATGTTCACAGAAGCAATAACGGTAAATGGACCGGAACAGCTGGGAAATATTCAAGTACCTAAAAGAGCCAGCTATATCAGAATAATTATGTTGGAGTTGAGTCGTATAGCTTCTCATCTGTTATGGCTCGGTCCTTTTATGGCGGATATTGGTGCACAGACTCCCTTTTTCTATATTTTCAGAGAAAGAGAATTAGTATATGATCTATTCGAAGCTGCCACCGGTATGAGAATGATGCATAATTATTTTCGGATCGGAGGGGTAGCGGCTGATCTCCCTCATGGCTGGATAGATAAATGTTTGGATTTCTGCGATTATTTTTTAATAAGGGTTGCTGAATATCAACAACTTATTACACGCAATCCTATTTTTTTAGAACGAGTCGAGGGGGTAGGCATTATTGGTCGAGAGGAAGTAATAAATTGGGGTTTATCGGGACCAATGCTGCGAGCGTCCGGAATACAATGGGATCTTCGTAAAGTTGATCAGTATGAGTGTTATGACGAATTTGATTGGGAAGTACAGTGGCAAAAAGAAGGGGATTCGTTGGCTCGTTATCTAGTCCGAATTGGTGAAATGATGGAATCCATAAAAATTATTCAACAGGCTCTCGAAGGAATTCCGGGGGGGCCATATGAGAATTTAGAAACCCGATACTTTGATAGAGAAAGGAATCCAGAATGGAATGATTTTGAATATCGCTTTATTAGTAAAAAACCTTCTCCTACATTTGAATTGCCGAAACAAGAACTTTATGTGAGAGTGGAAGCTCCAAAAGGGGAGTTGGGGGTTTTTCTGATAGGGGATCGGAGTGGTTTTCCTTGGAGATGGAAAATTCGACCGCCGGGTTTTATCAATTTGCAAATTCTTCCTCAGTTAGTTAAAAGAATGAAATTGGCTGATATTATGACAATACTAGGTAGTATAGATATCATTATGGGAGAAGTTGATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIN37

Protein Analysis

393

Amino Acids

45.77

Weight (kDa)

5.37

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 124 - 393 1.2e-102 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 680
AciI CCGC 4 cut(s) 164, 368, 491, 1067
AclWI GGATC 3 cut(s) 485, 702, 1038
AcsI RAATTY 5 cut(s) 731, 843, 865, 1059, 1089
AfaI GTAC 2 cut(s) 282, 747
AfiI CCNNNNNNNGG 2 cut(s) 131, 151
AflIII ACRYGT 1 cut(s) 203
AgeI ACCGGT 1 cut(s) 446
AgsI TTSAA 4 cut(s) 278, 827, 920, 959
AluBI AGCT 7 cut(s) 14, 221, 265, 297, 335, 440, 995
AluI AGCT 7 cut(s) 14, 221, 265, 297, 335, 440, 995
Alw21I GWGCWC 1 cut(s) 382
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 3 cut(s) 485, 702, 1038
Aor13HI TCCGGA 1 cut(s) 680
AoxI GGCC 1 cut(s) 854
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 2 cut(s) 440, 670
ApoI RAATTY 5 cut(s) 731, 843, 865, 1059, 1089
AsiGI ACCGGT 1 cut(s) 446
Asp700I GAANNNNTTC 1 cut(s) 843
AspS9I GGNCC 5 cut(s) 47, 254, 356, 662, 854
AsuC2I CCSGG 2 cut(s) 849, 1071
AsuHPI GGTGA 2 cut(s) 113, 809
AsuII TTCGAA 1 cut(s) 435
AvaII GGWCC 4 cut(s) 47, 254, 356, 662
BaeGI GKGCMC 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 928, 960
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 427, 657
BccI CCATC 6 cut(s) 63, 67, 92, 145, 799, 1047
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 2 cut(s) 849, 1071
BfaI CTAG 3 cut(s) 95, 785, 1142
BisI GCNGC 3 cut(s) 441, 492, 671
BlsI GCNGC 3 cut(s) 442, 493, 672
Bme1390I CCNGG 2 cut(s) 849, 1071
Bme18I GGWCC 4 cut(s) 47, 254, 356, 662
BmgT120I GGNCC 5 cut(s) 47, 254, 356, 662, 854
BmiI GGNNCC 4 cut(s) 48, 49, 663, 855
BmrFI CCNGG 2 cut(s) 849, 1071
BmsI GCATC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 435
BpuMI CCSGG 2 cut(s) 849, 1071
BsaJI CCNNGG 3 cut(s) 145, 848, 1046
BsaWI WCCGGW 3 cut(s) 256, 446, 680
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 151
Bse118I RCCGGY 1 cut(s) 446
BseAI TCCGGA 1 cut(s) 680
BseDI CCNNGG 3 cut(s) 145, 848, 1046
BseGI GGATG 2 cut(s) 55, 156
BseLI CCNNNNNNNGG 2 cut(s) 131, 151
BseMII CTCAG 1 cut(s) 1112
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 2 cut(s) 427, 657
BseYI CCCAGC 1 cut(s) 265
Bsh1285I CGRYCG 1 cut(s) 1067
BshFI GGCC 1 cut(s) 856
BshTI ACCGGT 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 5 cut(s) 257, 447, 681, 848, 1070
BslFI GGGAC 2 cut(s) 33, 675
BslI CCNNNNNNNGG 2 cut(s) 131, 151
BsmFI GGGAC 2 cut(s) 33, 675
BsnI GGCC 1 cut(s) 856
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 382
Bsp13I TCCGGA 1 cut(s) 680
Bsp143I GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
BspACI CCGC 4 cut(s) 164, 368, 491, 1067
BspANI GGCC 1 cut(s) 856
BspCNI CTCAG 1 cut(s) 1111
BspEI TCCGGA 1 cut(s) 680
BspLI GGNNCC 4 cut(s) 48, 49, 663, 855
BspPI GGATC 3 cut(s) 485, 702, 1038
BspT104I TTCGAA 1 cut(s) 435
BsrFI RCCGGY 1 cut(s) 446
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 3 cut(s) 145, 848, 1046
BssMI GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
BssT1I CCWWGG 1 cut(s) 1046
Bst4CI ACNGT 3 cut(s) 119, 247, 750
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 2 cut(s) 295, 675
BstDEI CTNAG 1 cut(s) 1098
BstF5I GGATG 2 cut(s) 55, 156
BstKTI GATC 7 cut(s) 430, 480, 499, 697, 712, 1033, 1177
BstMBI GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
BstMCI CGRYCG 1 cut(s) 1067
BstSCI CCNGG 2 cut(s) 847, 1069
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 2 cut(s) 427, 657
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BsuRI GGCC 1 cut(s) 856
BtsCI GGATG 2 cut(s) 55, 156
BtsIMutI CAGTG 1 cut(s) 755
Cac8I GCNNGC 2 cut(s) 295, 675
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 5 cut(s) 47, 254, 356, 662, 854
CseI GACGC 1 cut(s) 666
Csp6I GTAC 2 cut(s) 281, 746
CspAI ACCGGT 1 cut(s) 446
CviAII CATG 3 cut(s) 31, 68, 506
CviQI GTAC 2 cut(s) 281, 746
DdeI CTNAG 1 cut(s) 1098
DpnI GATC 7 cut(s) 429, 479, 498, 696, 711, 1032, 1176
DpnII GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
EciI GGCGGA 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 1046
Eco32I GATATC 1 cut(s) 1156
Eco47I GGWCC 4 cut(s) 47, 254, 356, 662
EcoO109I RGGNCCY 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 843
EcoRV GATATC 1 cut(s) 1156
EcoT14I CCWWGG 1 cut(s) 1046
EcoT22I ATGCAT 2 cut(s) 69, 465
ErhI CCWWGG 1 cut(s) 1046
FaeI CATG 3 cut(s) 34, 71, 509
FaqI GGGAC 2 cut(s) 33, 675
FatI CATG 3 cut(s) 30, 67, 505
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 1 cut(s) 709
Fnu4HI GCNGC 3 cut(s) 441, 492, 671
FokI GGATG 2 cut(s) 42, 163
Fsp4HI GCNGC 3 cut(s) 441, 492, 671
FspBI CTAG 3 cut(s) 95, 785, 1142
GluI GCNGC 3 cut(s) 441, 492, 671
GsaI CCCAGC 1 cut(s) 269
HaeIII GGCC 1 cut(s) 856
HapII CCGG 5 cut(s) 257, 447, 681, 848, 1070
HgaI GACGC 1 cut(s) 666
Hin1II CATG 3 cut(s) 34, 71, 509
HinfI GANTC 6 cut(s) 325, 385, 606, 767, 809, 898
HpaII CCGG 5 cut(s) 257, 447, 681, 848, 1070
HphI GGTGA 2 cut(s) 113, 809
Hpy166II GTNNAC 3 cut(s) 122, 231, 380
Hpy188I TCNGA 7 cut(s) 305, 407, 477, 482, 791, 1023, 1035
Hpy188III TCNNGA 6 cut(s) 95, 632, 660, 681, 836, 902
Hpy8I GTNNAC 3 cut(s) 122, 231, 380
HpyAV CCTTC 3 cut(s) 755, 833, 954
HpyCH4III ACNGT 3 cut(s) 119, 247, 750
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 4 cut(s) 67, 380, 463, 1087
HpyF3I CTNAG 1 cut(s) 1098
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 3 cut(s) 34, 71, 509
KflI GGGWCCC 1 cut(s) 47
Kpn2I TCCGGA 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
LmnI GCTCC 2 cut(s) 19, 1000
Lsp1109I GCAGC 2 cut(s) 427, 657
LweI GCATC 1 cut(s) 450
MaeI CTAG 3 cut(s) 95, 785, 1142
MaeII ACGT 1 cut(s) 205
MaeIII GTNAC 1 cut(s) 199
MalI GATC 7 cut(s) 429, 479, 498, 696, 711, 1032, 1176
MboI GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
MboII GAAGA 4 cut(s) 68, 116, 689, 1085
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 1 cut(s) 382
MlyI GAGTC 3 cut(s) 334, 379, 615
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 7 cut(s) 38, 140, 476, 513, 604, 628, 1107
Mph1103I ATGCAT 2 cut(s) 69, 465
MroI TCCGGA 1 cut(s) 680
MroXI GAANNNNTTC 1 cut(s) 843
MseI TTAA 2 cut(s) 548, 1107
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 5 cut(s) 257, 447, 681, 848, 1070
MspR9I CCNGG 2 cut(s) 849, 1071
NciI CCSGG 2 cut(s) 849, 1071
NdeI CATATG 1 cut(s) 859
NdeII GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
NlaIII CATG 3 cut(s) 34, 71, 509
NlaIV GGNNCC 4 cut(s) 48, 49, 663, 855
NsiI ATGCAT 2 cut(s) 69, 465
NspV TTCGAA 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 3 cut(s) 767, 809, 898
PinAI ACCGGT 1 cut(s) 446
PkrI GCNGC 3 cut(s) 442, 493, 672
PleI GAGTC 3 cut(s) 333, 379, 614
PpsI GAGTC 3 cut(s) 333, 379, 614
PpuMI RGGWCCY 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 47
PspFI CCCAGC 1 cut(s) 265
PspN4I GGNNCC 4 cut(s) 48, 49, 663, 855
PspPI GGNCC 5 cut(s) 47, 254, 356, 662, 854
PspPPI RGGWCCY 1 cut(s) 47
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 282, 747
RsaNI GTAC 2 cut(s) 281, 746
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 548, 1107
SatI GCNGC 3 cut(s) 441, 492, 671
Sau3AI GATC 7 cut(s) 427, 477, 496, 694, 709, 1030, 1174
Sau96I GGNCC 5 cut(s) 47, 254, 356, 662, 854
SchI GAGTC 3 cut(s) 334, 379, 615
ScrFI CCNGG 2 cut(s) 849, 1071
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 1 cut(s) 450
SfuI TTCGAA 1 cut(s) 435
SinI GGWCC 4 cut(s) 47, 254, 356, 662
SmiMI CAYNNNNRTG 1 cut(s) 449
SsiI CCGC 4 cut(s) 164, 368, 491, 1067
SspI AATATT 3 cut(s) 129, 188, 274
SspMI CTAG 3 cut(s) 95, 785, 1142
StyD4I CCNGG 2 cut(s) 847, 1069
StyI CCWWGG 1 cut(s) 1046
TaaI ACNGT 3 cut(s) 119, 247, 750
TaiI ACGT 1 cut(s) 208
TaqI TCGA 6 cut(s) 79, 435, 609, 631, 837, 1063
TaqII GACCGA 1 cut(s) 344
TatI WGTACW 1 cut(s) 745
TauI GCSGC 1 cut(s) 494
TfiI GAWTC 3 cut(s) 767, 809, 898
Tru1I TTAA 2 cut(s) 548, 1107
Tru9I TTAA 2 cut(s) 548, 1107
TscAI CASTG 1 cut(s) 755
TseI GCWGC 2 cut(s) 440, 670
TspDTI ATGAA 2 cut(s) 17, 1130
TspRI CASTG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 4 cut(s) 47, 254, 356, 662
XapI RAATTY 5 cut(s) 731, 843, 865, 1059, 1089
XbaI TCTAGA 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 3 cut(s) 95, 785, 1142
Zsp2I ATGCAT 2 cut(s) 69, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.