MD08G1006500.v1.1

U11 U12 small nuclear ribonucleoprotein 25 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003844
Physical Location & Seq
Reverse (-)
532478 .. 533938
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1006500.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGAGTCGGATGCGAAGGGGAAGGCGGAGATAAGCGTGGAAGGATACAAGGAAAGCAACATAAAGAAAGCCAGGTTGCAGTCGACGCTTGCTGCCCTTGTCAATGATCCTATACTTTCCGACGTTCCCAAAAAACCAACCTTATCCGACGTCGACACTCTCATCAGCCTCGAATTGGGCAGCGCCATGCGCATCTCCGTCCTCAAGCTCGACGGCACTTTCTTCGATGTGGCAGTGATGAATTCTGCAACGGTGAAGGACCTTAAGCTTGCAATCAGGAAGAAAATCAATGAGATGGAGCAATCCAAGATGGGTCACCGCCACATTTCATGGAAGCACGTTTGGAGGAATTTTTGCCTCTCATACCACAATGAGAAGCTACTTGATGACAATGCTCCAGTTCAGCATTTTGGCATACACAATCATTCTCAGGTGCATTTTGTTCCTCATATTGCTCTCAAGGATTCTCGTAAGCATTCGAAGAGGAGGAGACACCGCTTCTTTCACGGCCTCAGCAAGCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.97

Weight (kDa)

9.8

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 63 - 150 5.4e-32 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
Acc16I TGCGCA 1 cut(s) 191
AccI GTMKAC 2 cut(s) 83, 153
AciI CCGC 3 cut(s) 26, 319, 496
AclWI GGATC 1 cut(s) 101
AcsI RAATTY 2 cut(s) 241, 349
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 175
AflII CTTAAG 1 cut(s) 263
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 4 cut(s) 208, 268, 379, 520
AluI AGCT 4 cut(s) 208, 268, 379, 520
Alw26I GTCTC 1 cut(s) 484
AlwI GGATC 1 cut(s) 101
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 2 cut(s) 92, 180
ApoI RAATTY 2 cut(s) 241, 349
AspLEI GCGC 2 cut(s) 185, 192
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 2 cut(s) 265, 308
AsuII TTCGAA 1 cut(s) 479
AvaII GGWCC 1 cut(s) 259
BbvCI CCTCAGC 1 cut(s) 512
BbvI GCAGC 2 cut(s) 79, 192
BccI CCATC 2 cut(s) 289, 304
BceAI ACGGC 2 cut(s) 229, 523
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 73
BciVI GTATCC 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 484
BfoI RGCGCY 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 263
BfuI GTATCC 1 cut(s) 38
BisI GCNGC 2 cut(s) 93, 181
BlsI GCNGC 2 cut(s) 94, 182
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 512
Bpu14I TTCGAA 1 cut(s) 479
BpuEI CTTGAG 2 cut(s) 188, 443
BsaHI GRCGYC 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 398
BseBI CCWGG 1 cut(s) 73
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 443, 526
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 2 cut(s) 499, 502
BseXI GCAGC 2 cut(s) 79, 192
BshFI GGCC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 510
Bsp119I TTCGAA 1 cut(s) 479
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 3 cut(s) 26, 319, 496
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 2 cut(s) 442, 525
BspPI GGATC 1 cut(s) 101
BspT104I TTCGAA 1 cut(s) 479
BspTI CTTAAG 1 cut(s) 263
BsrI ACTGG 1 cut(s) 398
BssMI GATC 1 cut(s) 106
BssNI GRCGYC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 479
BstC8I GCNNGC 3 cut(s) 90, 270, 518
BstDEI CTNAG 2 cut(s) 429, 512
BstEII GGTNACC 1 cut(s) 314
BstF5I GGATG 1 cut(s) 16
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 185, 192
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 85, 189
BstNI CCWGG 1 cut(s) 73
BstPI GGTNACC 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 79, 192
BsuI GTATCC 1 cut(s) 38
BsuRI GGCC 1 cut(s) 510
BtsCI GGATG 1 cut(s) 16
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 3 cut(s) 90, 270, 518
CfoI GCGC 2 cut(s) 185, 192
Cfr13I GGNCC 1 cut(s) 259
CseI GACGC 1 cut(s) 94
CviAII CATG 2 cut(s) 187, 330
CviJI RGCY 7 cut(s) 71, 168, 208, 268, 379, 510, 520
CviKI_1 RGCY 7 cut(s) 71, 168, 208, 268, 379, 510, 520
DdeI CTNAG 2 cut(s) 429, 512
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
EciI GGCGGA 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 259
Eco91I GGTNACC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 259
EcoO65I GGTNACC 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 190, 333
FaiI YATR 7 cut(s) 62, 113, 188, 331, 364, 416, 450
FatI CATG 2 cut(s) 186, 329
FblI GTMKAC 2 cut(s) 83, 153
Fnu4HI GCNGC 2 cut(s) 93, 181
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 93, 181
FspAI RTGCGCAY 1 cut(s) 191
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 2 cut(s) 184, 191
GluI GCNGC 2 cut(s) 93, 181
GsuI CTGGAG 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 510
HgaI GACGC 1 cut(s) 94
HhaI GCGC 2 cut(s) 185, 192
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 2 cut(s) 190, 333
Hin6I GCGC 2 cut(s) 183, 190
HinP1I GCGC 2 cut(s) 183, 190
HincII GTYRAC 2 cut(s) 84, 154
HindII GTYRAC 2 cut(s) 84, 154
HindIII AAGCTT 2 cut(s) 266, 518
HinfI GANTC 2 cut(s) 5, 464
HphI GGTGA 2 cut(s) 265, 308
Hpy166II GTNNAC 2 cut(s) 84, 154
Hpy188I TCNGA 3 cut(s) 10, 121, 148
Hpy188III TCNNGA 2 cut(s) 277, 525
Hpy8I GTNNAC 2 cut(s) 84, 154
Hpy99I CGWCG 5 cut(s) 88, 125, 152, 155, 215
HpyAV CCTTC 4 cut(s) 10, 16, 35, 250
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 3 cut(s) 123, 150, 339
HpyCH4V TGCA 4 cut(s) 79, 248, 272, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 189
HpyF3I CTNAG 2 cut(s) 429, 512
HpySE526I ACGT 3 cut(s) 123, 150, 339
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 2 cut(s) 190, 333
HspAI GCGC 2 cut(s) 183, 190
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 2 cut(s) 298, 400
LpnPI CCDG 5 cut(s) 58, 85, 262, 411, 416
Lsp1109I GCAGC 2 cut(s) 79, 192
LweI GCATC 1 cut(s) 201
MaeII ACGT 3 cut(s) 123, 150, 339
MaeIII GTNAC 1 cut(s) 314
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 3 cut(s) 214, 292, 493
MluCI AATT 3 cut(s) 173, 241, 349
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 144, 171
MnlI CCTC 8 cut(s) 179, 212, 339, 368, 456, 477, 480, 521
MseI TTAA 1 cut(s) 264
MspCI CTTAAG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 85, 189
NdeII GATC 1 cut(s) 106
NlaIII CATG 2 cut(s) 190, 333
NmuCI GTSAC 1 cut(s) 314
NsbI TGCGCA 1 cut(s) 191
NspV TTCGAA 1 cut(s) 479
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 464
PkrI GCNGC 2 cut(s) 94, 182
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 71
PspEI GGTNACC 1 cut(s) 314
PspGI CCWGG 1 cut(s) 71
PspPI GGNCC 1 cut(s) 259
PspPPI RGGWCCY 1 cut(s) 259
SalI GTCGAC 2 cut(s) 82, 152
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 93, 181
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 73
SfaNI GCATC 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 479
SinI GGWCC 1 cut(s) 259
SmlI CTYRAG 3 cut(s) 203, 263, 458
SmoI CTYRAG 3 cut(s) 203, 263, 458
Sse9I AATT 3 cut(s) 173, 241, 349
SsiI CCGC 3 cut(s) 26, 319, 496
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 3 cut(s) 126, 153, 342
TaqI TCGA 6 cut(s) 83, 153, 171, 210, 225, 479
TasI AATT 3 cut(s) 173, 241, 349
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 1 cut(s) 240
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 92, 180
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 2 cut(s) 254, 318
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 1 cut(s) 240
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 2 cut(s) 241, 349
XmiI GTMKAC 2 cut(s) 83, 153
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.