Rw6G038010

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003844
Physical Location & Seq
Forward (+)
62247444 .. 62250094
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038010.1

Sequence Viewer

Length: 969 bp
ATGGCTACTTCGGTGAAAGTGTACGGACCACCGATATCCACGGCCGTGTCGAGAGTGTTGGCCTGTCTCCATGAGAAAGAAGTGCCCTATCAGCTCATTCCGGTCAACATGGCCAAAGGCGAGCACAAGAAGCCTAAATACCTTAAGATCCAGCCATTTGGACAAGTGCCAGCTTTTGAAGACGAGGGCTTCGCCCTTTTCGAATCGAGGTCCATATGCCGGTACATATGTGAGAAGTATGCAGAGCAGGGAAACAAGGGATTGTATGGGGAAAACCCATTGGCAAAGGCCTCCATAGATCAGTGGCTAGAAGCTGAATCACATAGCTTCAGTCCTCCAAGCTCCGTTCTGGTGTTTCAGCTTGCATTCGCACCGAGAATGAAGCTGAAGCAGGATCAAGGGGCGATCCGGCAGAACGAAGAGAAGCTGAAGAAGGTGCTGGACGTGTACGAAGAGAGGCTGGGTGAGAGCCGGTTCTTGGCGGGATACAAGGAAAGCAACATAAAGAAAGCAATGTTACAGTCGACGCTGGCCGCCCTCTTGGACGATCCCATCCTTTCCGACGTCCCCAAAAAACCGACCTTATCTGATGTCGACACTCTCCTCAGCCTCGAATTCGGCAGCGCCATGCGCATCAGTGTCGTCAAGCTTGACGCCACCTCTTTCGATGTGGCGCTCATGAACTCTGCTACGGTCAAGGATCTCAAGCTTGCGATAAAAAAGAAAGTCAACGAAATGGAGCAGTCCAAGATGGGTCACCGCCACATTTCATGGAAGCATGTGTGGGGGAATTTTTGCCTGTCATACCACAATGAGAAGTTGCTGGATGACAATGCCGCAGTTGAGGAGTACGGCATTCGCAACAATTCTCAGGTGCATTTTGTTCCTTGTGTGGCGTCCAAGGGTTCTCGGAGGCATTCAAAGAGAAGGAGACATCGCTTCTTTCATGGTCTTAGCAAGCGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_N

Protein Analysis

322

Amino Acids

36.47

Weight (kDa)

9.34

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 4 - 76 9e-18 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 8 - 82 4.8e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 78 2.5e-15 Glutathione S-transferase, N-terminal domain
Ubiquitin_4 PF18036 210 - 296 2.1e-31 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 567
Acc16I TGCGCA 1 cut(s) 632
AccI GTMKAC 2 cut(s) 524, 594
AciI CCGC 4 cut(s) 482, 534, 760, 837
AclWI GGATC 5 cut(s) 142, 400, 402, 542, 708
AcoI YGGCCR 3 cut(s) 42, 111, 531
AcsI RAATTY 2 cut(s) 614, 790
AcuI CTGAAG 3 cut(s) 313, 407, 449
AcyI GRCGYC 3 cut(s) 564, 654, 896
AfaI GTAC 4 cut(s) 23, 224, 449, 851
AfiI CCNNNNNNNGG 2 cut(s) 219, 478
AflII CTTAAG 1 cut(s) 143
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 2 cut(s) 179, 921
AjiI CACGTC 1 cut(s) 445
AleI CACNNNNGTG 1 cut(s) 44
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 71, 925
AlwI GGATC 5 cut(s) 142, 400, 402, 542, 708
AoxI GGCC 5 cut(s) 42, 60, 111, 288, 531
ApeKI GCWGC 1 cut(s) 621
ApoI RAATTY 2 cut(s) 614, 790
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AspLEI GCGC 3 cut(s) 626, 633, 676
AspS9I GGNCC 2 cut(s) 26, 210
AsuHPI GGTGA 3 cut(s) 25, 476, 749
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 2 cut(s) 26, 210
BaeGI GKGCMC 1 cut(s) 87
BaeI ACNNNNGTAYC 2 cut(s) 214, 247
BalI TGGCCA 1 cut(s) 113
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 605
BbvI GCAGC 1 cut(s) 633
BccI CCATC 2 cut(s) 560, 745
BceAI ACGGC 3 cut(s) 29, 57, 868
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciVI GTATCC 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 71, 925
BfaI CTAG 1 cut(s) 308
BfoI RGCGCY 2 cut(s) 627, 677
BfrI CTTAAG 1 cut(s) 143
BfuI GTATCC 1 cut(s) 479
BisI GCNGC 3 cut(s) 534, 622, 837
BlsI GCNGC 3 cut(s) 535, 623, 838
Bme18I GGWCC 2 cut(s) 26, 210
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 26, 210
BmsI GCATC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 605
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 689
BsaHI GRCGYC 3 cut(s) 564, 654, 896
BsaJI CCNNGG 2 cut(s) 39, 900
BsaWI WCCGGW 1 cut(s) 100
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 478
Bse118I RCCGGY 2 cut(s) 219, 471
Bse3DI GCAATG 1 cut(s) 519
BseDI CCNNGG 2 cut(s) 39, 900
BseGI GGATG 2 cut(s) 552, 832
BseLI CCNNNNNNNGG 2 cut(s) 219, 478
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 619, 884
BseRI GAGGAG 2 cut(s) 593, 860
BseSI GKGCMC 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 633
BseYI CCCAGC 1 cut(s) 460
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 44, 62, 113, 290, 533
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 4 cut(s) 101, 220, 409, 472
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 219, 478
BsmAI GTCTC 2 cut(s) 71, 925
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 3 cut(s) 365, 855, 916
BsnI GGCC 5 cut(s) 44, 62, 113, 290, 533
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 87, 126
Bsp143I GATC 6 cut(s) 147, 298, 394, 405, 547, 700
BspACI CCGC 4 cut(s) 482, 534, 760, 837
BspANI GGCC 5 cut(s) 44, 62, 113, 290, 533
BspCNI CTCAG 2 cut(s) 618, 883
BspHI TCATGA 1 cut(s) 678
BspPI GGATC 5 cut(s) 142, 400, 402, 542, 708
BspT104I TTCGAA 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 219, 471
BssAI RCCGGY 2 cut(s) 219, 471
BssECI CCNNGG 2 cut(s) 39, 900
BssMI GATC 6 cut(s) 147, 298, 394, 405, 547, 700
BssNI GRCGYC 3 cut(s) 564, 654, 896
BssT1I CCWWGG 1 cut(s) 900
Bst4CI ACNGT 2 cut(s) 522, 694
Bst6I CTCTTC 2 cut(s) 414, 447
BstACI GRCGYC 3 cut(s) 564, 654, 896
BstAFI CTTAAG 1 cut(s) 143
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 6 cut(s) 122, 171, 363, 531, 711, 959
BstDEI CTNAG 3 cut(s) 605, 870, 953
BstDSI CCRYGG 1 cut(s) 39
BstEII GGTNACC 1 cut(s) 755
BstF5I GGATG 2 cut(s) 552, 832
BstH2I RGCGCY 2 cut(s) 627, 677
BstHHI GCGC 3 cut(s) 626, 633, 676
BstKTI GATC 6 cut(s) 150, 301, 397, 408, 550, 703
BstMAI GTCTC 2 cut(s) 71, 925
BstMBI GATC 6 cut(s) 147, 298, 394, 405, 547, 700
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 91, 130, 630
BstNSI RCATGY 1 cut(s) 782
BstPI GGTNACC 1 cut(s) 755
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 633
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 2 cut(s) 147, 700
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 2 cut(s) 147, 700
BstZI CGGCCG 1 cut(s) 42
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 5 cut(s) 44, 62, 113, 290, 533
BtgI CCRYGG 1 cut(s) 39
BtgZI GCGATG 1 cut(s) 920
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 552, 832
BtsIMutI CAGTG 2 cut(s) 308, 643
Cac8I GCNNGC 6 cut(s) 122, 171, 363, 531, 711, 959
CciI TCATGA 1 cut(s) 678
CfoI GCGC 3 cut(s) 626, 633, 676
Cfr10I RCCGGY 2 cut(s) 219, 471
Cfr13I GGNCC 2 cut(s) 26, 210
CseI GACGC 3 cut(s) 535, 662, 885
Csp6I GTAC 4 cut(s) 22, 223, 448, 850
CviAII CATG 7 cut(s) 71, 109, 628, 679, 771, 779, 947
CviQI GTAC 4 cut(s) 22, 223, 448, 850
DdeI CTNAG 3 cut(s) 605, 870, 953
DpnI GATC 6 cut(s) 149, 300, 396, 407, 549, 702
DpnII GATC 6 cut(s) 147, 298, 394, 405, 547, 700
EaeI YGGCCR 3 cut(s) 42, 111, 531
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 414, 447
EarI CTCTTC 2 cut(s) 414, 447
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 900
Eco147I AGGCCT 1 cut(s) 290
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 2 cut(s) 26, 210
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 3 cut(s) 313, 407, 449
Eco91I GGTNACC 1 cut(s) 755
EcoO65I GGTNACC 1 cut(s) 755
EcoRI GAATTC 1 cut(s) 614
EcoRV GATATC 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 900
ErhI CCWWGG 1 cut(s) 900
FaeI CATG 7 cut(s) 74, 112, 631, 682, 774, 782, 950
FaqI GGGAC 1 cut(s) 551
FatI CATG 7 cut(s) 70, 108, 627, 678, 770, 778, 946
FauI CCCGC 1 cut(s) 475
FauNDI CATATG 2 cut(s) 215, 227
FblI GTMKAC 2 cut(s) 524, 594
Fnu4HI GCNGC 3 cut(s) 534, 622, 837
FokI GGATG 2 cut(s) 539, 839
Fsp4HI GCNGC 3 cut(s) 534, 622, 837
FspAI RTGCGCAY 1 cut(s) 632
FspBI CTAG 1 cut(s) 308
FspI TGCGCA 1 cut(s) 632
GlaI GCGC 3 cut(s) 625, 632, 675
GluI GCNGC 3 cut(s) 534, 622, 837
GsaI CCCAGC 1 cut(s) 464
HaeII RGCGCY 2 cut(s) 627, 677
HaeIII GGCC 5 cut(s) 44, 62, 113, 290, 533
HapII CCGG 4 cut(s) 101, 220, 409, 472
HgaI GACGC 3 cut(s) 535, 662, 885
HhaI GCGC 3 cut(s) 626, 633, 676
Hin1I GRCGYC 3 cut(s) 564, 654, 896
Hin1II CATG 7 cut(s) 74, 112, 631, 682, 774, 782, 950
Hin6I GCGC 3 cut(s) 624, 631, 674
HinP1I GCGC 3 cut(s) 624, 631, 674
HincII GTYRAC 4 cut(s) 106, 525, 595, 730
HindII GTYRAC 4 cut(s) 106, 525, 595, 730
HindIII AAGCTT 2 cut(s) 647, 707
HinfI GANTC 2 cut(s) 203, 317
HpaII CCGG 4 cut(s) 101, 220, 409, 472
HphI GGTGA 3 cut(s) 25, 476, 749
Hpy166II GTNNAC 6 cut(s) 22, 106, 448, 525, 595, 730
Hpy188I TCNGA 3 cut(s) 562, 589, 912
Hpy188III TCNNGA 2 cut(s) 51, 679
Hpy8I GTNNAC 6 cut(s) 22, 106, 448, 525, 595, 730
Hpy99I CGWCG 2 cut(s) 529, 566
HpyAV CCTTC 2 cut(s) 427, 921
HpyCH4III ACNGT 2 cut(s) 522, 694
HpyCH4IV ACGT 2 cut(s) 444, 564
HpyCH4V TGCA 3 cut(s) 242, 365, 877
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 130, 630
HpyF3I CTNAG 3 cut(s) 605, 870, 953
HpySE526I ACGT 2 cut(s) 444, 564
Hsp92I GRCGYC 3 cut(s) 564, 654, 896
Hsp92II CATG 7 cut(s) 74, 112, 631, 682, 774, 782, 950
HspAI GCGC 3 cut(s) 624, 631, 674
Kzo9I GATC 6 cut(s) 147, 298, 394, 405, 547, 700
LmnI GCTCC 2 cut(s) 347, 739
Lsp1109I GCAGC 1 cut(s) 633
LweI GCATC 1 cut(s) 642
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 444, 564
MaeIII GTNAC 2 cut(s) 516, 755
MalI GATC 6 cut(s) 149, 300, 396, 407, 549, 702
MboI GATC 6 cut(s) 147, 298, 394, 405, 547, 700
MboII GAAGA 4 cut(s) 191, 431, 442, 464
MflI RGATCY 2 cut(s) 147, 700
MhlI GDGCHC 2 cut(s) 87, 126
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 3 cut(s) 614, 790, 865
MluNI TGGCCA 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 585
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 113
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 4 cut(s) 101, 220, 409, 472
Mva1269I GAATGC 3 cut(s) 365, 855, 916
MwoI GCNNNNNNNGC 3 cut(s) 91, 130, 630
NdeI CATATG 2 cut(s) 215, 227
NdeII GATC 6 cut(s) 147, 298, 394, 405, 547, 700
NlaIII CATG 7 cut(s) 74, 112, 631, 682, 774, 782, 950
NmuCI GTSAC 1 cut(s) 755
NsbI TGCGCA 1 cut(s) 632
NspI RCATGY 1 cut(s) 782
NspV TTCGAA 1 cut(s) 201
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 678
PceI AGGCCT 1 cut(s) 290
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 198
PctI GAATGC 3 cut(s) 365, 855, 916
PfeI GAWTC 2 cut(s) 203, 317
PkrI GCNGC 3 cut(s) 535, 623, 838
PspEI GGTNACC 1 cut(s) 755
PspFI CCCAGC 1 cut(s) 460
PspPI GGNCC 2 cut(s) 26, 210
PsuI RGATCY 2 cut(s) 147, 700
RsaI GTAC 4 cut(s) 23, 224, 449, 851
RsaNI GTAC 4 cut(s) 22, 223, 448, 850
RseI CAYNNNNRTG 1 cut(s) 44
SalI GTCGAC 2 cut(s) 523, 593
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 534, 622, 837
Sau3AI GATC 6 cut(s) 147, 298, 394, 405, 547, 700
Sau96I GGNCC 2 cut(s) 26, 210
SduI GDGCHC 2 cut(s) 87, 126
SfaNI GCATC 1 cut(s) 642
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 2 cut(s) 26, 210
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 143, 704
SmoI CTYRAG 2 cut(s) 143, 704
Sse9I AATT 3 cut(s) 614, 790, 865
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 4 cut(s) 482, 534, 760, 837
SspMI CTAG 1 cut(s) 308
StuI AGGCCT 1 cut(s) 290
StyI CCWWGG 1 cut(s) 900
TaaI ACNGT 2 cut(s) 522, 694
TaiI ACGT 2 cut(s) 447, 567
TaqI TCGA 7 cut(s) 50, 201, 206, 524, 594, 612, 666
TasI AATT 3 cut(s) 614, 790, 865
TauI GCSGC 2 cut(s) 536, 839
TfiI GAWTC 2 cut(s) 203, 317
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 2 cut(s) 308, 643
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 1 cut(s) 621
Tsp45I GTSAC 1 cut(s) 755
TspDTI ATGAA 4 cut(s) 395, 695, 759, 935
TspGWI ACGGA 2 cut(s) 39, 334
TspRI CASTG 2 cut(s) 308, 643
Vha464I CTTAAG 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 26, 210
XapI RAATTY 2 cut(s) 614, 790
XceI RCATGY 1 cut(s) 782
XmiI GTMKAC 2 cut(s) 524, 594
XspI CTAG 1 cut(s) 308
ZraI GACGTC 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.