Rh6BG463900

U11 U12 small nuclear ribonucleoprotein 25 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003844
Physical Location & Seq
Reverse (-)
67350401 .. 67351275
875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG463900.1

Sequence Viewer

Length: 528 bp
ATGGAGTCCAAAACGACGCCGGAAGCTAGCGTTAGCTTGGAAGGATACAAGGAAAGCAACATAAAGAAAGCAAGGCTACAGTCAACGCTGGCCGCCCTCTTGGAAGATCCCATCCTTTCCGACGTCCCCAAAAAACCGACCTTATCGGATGTCGACACTCTCCTCAGCCTCGAATTCGGCAGCGCCATGCGCATCAATGTCGTCAAGCTTGACGCCACCTCTTTGGATGTGGCGCTCATGAACTCTGCCACGGTCAAGGATCTCAAGCTTGCGATAAAAAAGAAAGTCAACGAAATGGAGCAGTCCAAGATGGGTCACCGCCACATTTCATGGAAGCATGTGTGGGGAAATTTTTGCCTGTCATACCACAATGAGAAGTTGCTGGATGACAATGCTGCAGTTGAGGAGTATGGCATTCGCAACAATTCTCAGGTCCATTTTGTTCCTTGTGTGGCATCCAAGGGTTCTAGGAGGCATTCAAAGAGGAGGAGACATCGCTTCTTTCATGGTCTTAGCAAGCGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.82

Weight (kDa)

9.64

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 63 - 149 4.4e-32 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
Acc16I TGCGCA 1 cut(s) 191
AccI GTMKAC 1 cut(s) 153
AciI CCGC 2 cut(s) 93, 319
AclWI GGATC 2 cut(s) 101, 267
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 2 cut(s) 173, 349
AcyI GRCGYC 3 cut(s) 17, 123, 213
AgsI TTSAA 1 cut(s) 480
AluBI AGCT 4 cut(s) 26, 36, 208, 268
AluI AGCT 4 cut(s) 26, 36, 208, 268
Alw26I GTCTC 1 cut(s) 484
AlwI GGATC 2 cut(s) 101, 267
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 2 cut(s) 180, 395
ApoI RAATTY 2 cut(s) 173, 349
AspLEI GCGC 3 cut(s) 185, 192, 235
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 308
AsuNHI GCTAGC 1 cut(s) 26
AvaII GGWCC 1 cut(s) 433
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 2 cut(s) 192, 382
BccI CCATC 2 cut(s) 119, 304
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciVI GTATCC 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 484
BfaI CTAG 2 cut(s) 27, 468
BfmI CTRYAG 2 cut(s) 77, 396
BfoI RGCGCY 2 cut(s) 186, 236
BfuI GTATCC 1 cut(s) 38
BisI GCNGC 3 cut(s) 93, 181, 396
BlsI GCNGC 3 cut(s) 94, 182, 397
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 433
BmsI GCATC 2 cut(s) 201, 464
BmtI GCTAGC 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 248
BsaHI GRCGYC 3 cut(s) 17, 123, 213
BsaJI CCNNGG 2 cut(s) 249, 459
BseDI CCNNGG 2 cut(s) 249, 459
BseGI GGATG 5 cut(s) 111, 154, 232, 391, 455
BseMII CTCAG 2 cut(s) 178, 443
BseRI GAGGAG 4 cut(s) 152, 419, 499, 502
BseXI GCAGC 2 cut(s) 192, 382
BshFI GGCC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 110
BsmAI GTCTC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 2 cut(s) 414, 475
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 106, 259
BspACI CCGC 2 cut(s) 93, 319
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 2 cut(s) 177, 442
BspHI TCATGA 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 400
BspOI GCTAGC 1 cut(s) 30
BspPI GGATC 2 cut(s) 101, 267
BssECI CCNNGG 2 cut(s) 249, 459
BssMI GATC 2 cut(s) 106, 259
BssNI GRCGYC 3 cut(s) 17, 123, 213
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 2 cut(s) 81, 253
BstACI GRCGYC 3 cut(s) 17, 123, 213
BstC8I GCNNGC 4 cut(s) 28, 90, 270, 518
BstDEI CTNAG 3 cut(s) 164, 429, 512
BstDSI CCRYGG 1 cut(s) 249
BstEII GGTNACC 1 cut(s) 314
BstF5I GGATG 5 cut(s) 111, 154, 232, 391, 455
BstH2I RGCGCY 2 cut(s) 186, 236
BstHHI GCGC 3 cut(s) 185, 192, 235
BstKTI GATC 2 cut(s) 109, 262
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 2 cut(s) 106, 259
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 341
BstPI GGTNACC 1 cut(s) 314
BstSFI CTRYAG 2 cut(s) 77, 396
BstV1I GCAGC 2 cut(s) 192, 382
BstX2I RGATCY 2 cut(s) 106, 259
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 106, 259
BsuI GTATCC 1 cut(s) 38
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 479
BtsCI GGATG 5 cut(s) 111, 154, 232, 391, 455
Cac8I GCNNGC 4 cut(s) 28, 90, 270, 518
CciI TCATGA 1 cut(s) 237
CfoI GCGC 3 cut(s) 185, 192, 235
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 2 cut(s) 25, 221
CviAII CATG 5 cut(s) 187, 238, 330, 338, 506
CviJI RGCY 7 cut(s) 26, 36, 76, 92, 168, 208, 268
CviKI_1 RGCY 7 cut(s) 26, 36, 76, 92, 168, 208, 268
DdeI CTNAG 3 cut(s) 164, 429, 512
DpnI GATC 2 cut(s) 108, 261
DpnII GATC 2 cut(s) 106, 259
EaeI YGGCCR 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 433
Eco91I GGTNACC 1 cut(s) 314
EcoO65I GGTNACC 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 5 cut(s) 190, 241, 333, 341, 509
FaiI YATR 8 cut(s) 62, 188, 239, 331, 339, 364, 411, 507
FaqI GGGAC 1 cut(s) 110
FatI CATG 5 cut(s) 186, 237, 329, 337, 505
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 93, 181, 396
FokI GGATG 5 cut(s) 98, 161, 239, 398, 442
Fsp4HI GCNGC 3 cut(s) 93, 181, 396
FspAI RTGCGCAY 1 cut(s) 191
FspBI CTAG 2 cut(s) 27, 468
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 3 cut(s) 184, 191, 234
GluI GCNGC 3 cut(s) 93, 181, 396
HaeII RGCGCY 2 cut(s) 186, 236
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 20
HgaI GACGC 2 cut(s) 25, 221
HhaI GCGC 3 cut(s) 185, 192, 235
Hin1I GRCGYC 3 cut(s) 17, 123, 213
Hin1II CATG 5 cut(s) 190, 241, 333, 341, 509
Hin6I GCGC 3 cut(s) 183, 190, 233
HinP1I GCGC 3 cut(s) 183, 190, 233
HincII GTYRAC 3 cut(s) 84, 154, 289
HindII GTYRAC 3 cut(s) 84, 154, 289
HindIII AAGCTT 2 cut(s) 206, 266
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 20
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 3 cut(s) 84, 154, 289
Hpy188I TCNGA 2 cut(s) 121, 148
Hpy188III TCNNGA 1 cut(s) 238
Hpy8I GTNNAC 3 cut(s) 84, 154, 289
Hpy99I CGWCG 2 cut(s) 19, 125
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 2 cut(s) 81, 253
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 1 cut(s) 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 3 cut(s) 164, 429, 512
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 3 cut(s) 17, 123, 213
Hsp92II CATG 5 cut(s) 190, 241, 333, 341, 509
HspAI GCGC 3 cut(s) 183, 190, 233
Kzo9I GATC 2 cut(s) 106, 259
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 5 cut(s) 33, 74, 368, 371, 416
Lsp1109I GCAGC 2 cut(s) 192, 382
LweI GCATC 2 cut(s) 201, 464
MaeI CTAG 2 cut(s) 27, 468
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 314
MalI GATC 2 cut(s) 108, 261
MboI GATC 2 cut(s) 106, 259
MboII GAAGA 1 cut(s) 116
MflI RGATCY 2 cut(s) 106, 259
MluCI AATT 3 cut(s) 173, 349, 424
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 8 cut(s) 107, 173, 179, 229, 397, 465, 477, 480
MspI CCGG 1 cut(s) 20
Mva1269I GAATGC 2 cut(s) 414, 475
MwoI GCNNNNNNNGC 1 cut(s) 189
NdeII GATC 2 cut(s) 106, 259
NheI GCTAGC 1 cut(s) 26
NlaIII CATG 5 cut(s) 190, 241, 333, 341, 509
NmuCI GTSAC 1 cut(s) 314
NsbI TGCGCA 1 cut(s) 191
NspI RCATGY 1 cut(s) 341
PagI TCATGA 1 cut(s) 237
PctI GAATGC 2 cut(s) 414, 475
PkrI GCNGC 3 cut(s) 94, 182, 397
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PspEI GGTNACC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 433
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 2 cut(s) 106, 259
SalI GTCGAC 1 cut(s) 152
SatI GCNGC 3 cut(s) 93, 181, 396
Sau3AI GATC 2 cut(s) 106, 259
Sau96I GGNCC 1 cut(s) 433
SchI GAGTC 1 cut(s) 14
SetI ASST 8 cut(s) 28, 38, 126, 143, 210, 221, 270, 435
SfaNI GCATC 2 cut(s) 201, 464
SfcI CTRYAG 2 cut(s) 77, 396
SinI GGWCC 1 cut(s) 433
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 3 cut(s) 173, 349, 424
SsiI CCGC 2 cut(s) 93, 319
SspMI CTAG 2 cut(s) 27, 468
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 81, 253
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 153, 171
TasI AATT 3 cut(s) 173, 349, 424
TauI GCSGC 1 cut(s) 95
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 180, 395
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 3 cut(s) 254, 318, 494
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 2 cut(s) 173, 349
XceI RCATGY 1 cut(s) 341
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 2 cut(s) 27, 468
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.