RchiOBHm_Chr6g0303781

U11 U12 small nuclear ribonucleoprotein 25 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003844
Physical Location & Seq
Forward (+)
63380623 .. 63381805
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27292

Sequence Viewer

Length: 561 bp
ATGACCGAGACTGAACAGAAGAGCAACATCAACATGGAGTCCACAACGACGCCGGAAGCTAGCGTTAGCTTGGAAGGATACAAGGAAAGCAACATAAAGAAAGCAAGGTTACAGTCGACGCTGGCCGCCCTCTTGGACGATCCCATCCTTTCCGACGTCCCCCAAAAACCGACCTTATCTGATGTCGACACTCTCCTCAGCCTCGAATTCGGCAGCGCCATGCGCATCAGTGTCGTCAAGCTTGACGCCACCTCTTTCGATGTAGCGCTCATGAACTCTGCTACGGTCAAGGATCTCAAGCTTGCGATAAAAAAGAAAGTCAACGAAATGGAGCAGTCCAAGATGGGTCACCGCCACATTTCATGGAAGCATGTGTGGGGGAATTTTTGCCTGTCATACCACAATGAGAAGTTGCTGGATGACAATGCCGCAGTTGAGGAGTACGGCATTCGCAACAATTCTCAGGTGCATTTTGTTCCTTGTGTGGCGTCCAAGGGTTCTCGGAGGCATTCAAAGAGGAGGAGACATCGCTTCTTTCATGGTCTTAGCAAGCGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

21.06

Weight (kDa)

9.34

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 74 - 160 6.3e-32 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
Acc16I TGCGCA 1 cut(s) 224
AccI GTMKAC 2 cut(s) 116, 186
AciI CCGC 3 cut(s) 126, 352, 429
AclWI GGATC 2 cut(s) 134, 300
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 2 cut(s) 206, 382
AcyI GRCGYC 4 cut(s) 50, 156, 246, 488
AfaI GTAC 1 cut(s) 443
AfeI AGCGCT 1 cut(s) 267
AgsI TTSAA 1 cut(s) 513
AluBI AGCT 4 cut(s) 59, 69, 241, 301
AluI AGCT 4 cut(s) 59, 69, 241, 301
Alw26I GTCTC 2 cut(s) 2, 517
AlwI GGATC 2 cut(s) 134, 300
Aor51HI AGCGCT 1 cut(s) 267
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 1 cut(s) 213
ApoI RAATTY 2 cut(s) 206, 382
AspLEI GCGC 3 cut(s) 218, 225, 268
AsuHPI GGTGA 1 cut(s) 341
AsuNHI GCTAGC 1 cut(s) 59
BbvCI CCTCAGC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 225
BccI CCATC 2 cut(s) 152, 337
BceAI ACGGC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciVI GTATCC 1 cut(s) 71
BcoDI GTCTC 2 cut(s) 2, 517
BfaI CTAG 1 cut(s) 60
BfoI RGCGCY 2 cut(s) 219, 269
BfuI GTATCC 1 cut(s) 71
BisI GCNGC 3 cut(s) 126, 214, 429
BlsI GCNGC 3 cut(s) 127, 215, 430
BmsI GCATC 1 cut(s) 234
BmtI GCTAGC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 281
BsaHI GRCGYC 4 cut(s) 50, 156, 246, 488
BsaJI CCNNGG 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 2 cut(s) 144, 424
BseMII CTCAG 2 cut(s) 211, 476
BseRI GAGGAG 4 cut(s) 185, 452, 532, 535
BseXI GCAGC 1 cut(s) 225
BshFI GGCC 1 cut(s) 125
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 2, 517
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 2 cut(s) 447, 508
BsnI GGCC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 139, 292
BspACI CCGC 3 cut(s) 126, 352, 429
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 2 cut(s) 210, 475
BspHI TCATGA 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 63
BspPI GGATC 2 cut(s) 134, 300
BspQI GCTCTTC 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 2 cut(s) 139, 292
BssNI GRCGYC 4 cut(s) 50, 156, 246, 488
BssT1I CCWWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 114, 286
Bst6I CTCTTC 1 cut(s) 14
BstACI GRCGYC 4 cut(s) 50, 156, 246, 488
BstC8I GCNNGC 4 cut(s) 61, 123, 303, 551
BstDEI CTNAG 3 cut(s) 197, 462, 545
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 2 cut(s) 144, 424
BstH2I RGCGCY 2 cut(s) 219, 269
BstHHI GCGC 3 cut(s) 218, 225, 268
BstKTI GATC 2 cut(s) 142, 295
BstMAI GTCTC 2 cut(s) 2, 517
BstMBI GATC 2 cut(s) 139, 292
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 374
BstPI GGTNACC 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuI GTATCC 1 cut(s) 71
BsuRI GGCC 1 cut(s) 125
BtgZI GCGATG 1 cut(s) 512
BtsCI GGATG 2 cut(s) 144, 424
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 4 cut(s) 61, 123, 303, 551
CciI TCATGA 1 cut(s) 270
CfoI GCGC 3 cut(s) 218, 225, 268
CseI GACGC 4 cut(s) 58, 127, 254, 477
Csp6I GTAC 1 cut(s) 442
CviAII CATG 6 cut(s) 34, 220, 271, 363, 371, 539
CviJI RGCY 6 cut(s) 59, 69, 125, 201, 241, 301
CviKI_1 RGCY 6 cut(s) 59, 69, 125, 201, 241, 301
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 3 cut(s) 197, 462, 545
DpnI GATC 2 cut(s) 141, 294
DpnII GATC 2 cut(s) 139, 292
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 492
Eco47III AGCGCT 1 cut(s) 267
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoRI GAATTC 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 6 cut(s) 37, 223, 274, 366, 374, 542
FaiI YATR 8 cut(s) 35, 95, 221, 272, 364, 372, 397, 540
FaqI GGGAC 1 cut(s) 143
FatI CATG 6 cut(s) 33, 219, 270, 362, 370, 538
FblI GTMKAC 2 cut(s) 116, 186
Fnu4HI GCNGC 3 cut(s) 126, 214, 429
FokI GGATG 2 cut(s) 131, 431
Fsp4HI GCNGC 3 cut(s) 126, 214, 429
FspAI RTGCGCAY 1 cut(s) 224
FspBI CTAG 1 cut(s) 60
FspI TGCGCA 1 cut(s) 224
GlaI GCGC 3 cut(s) 217, 224, 267
GluI GCNGC 3 cut(s) 126, 214, 429
HaeII RGCGCY 2 cut(s) 219, 269
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 53
HgaI GACGC 4 cut(s) 58, 127, 254, 477
HhaI GCGC 3 cut(s) 218, 225, 268
Hin1I GRCGYC 4 cut(s) 50, 156, 246, 488
Hin1II CATG 6 cut(s) 37, 223, 274, 366, 374, 542
Hin6I GCGC 3 cut(s) 216, 223, 266
HinP1I GCGC 3 cut(s) 216, 223, 266
HincII GTYRAC 3 cut(s) 117, 187, 322
HindII GTYRAC 3 cut(s) 117, 187, 322
HindIII AAGCTT 2 cut(s) 239, 299
HinfI GANTC 1 cut(s) 38
HpaII CCGG 1 cut(s) 53
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 4 cut(s) 42, 117, 187, 322
Hpy188I TCNGA 3 cut(s) 154, 181, 504
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 4 cut(s) 42, 117, 187, 322
Hpy99I CGWCG 3 cut(s) 52, 121, 158
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 2 cut(s) 114, 286
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 3 cut(s) 197, 462, 545
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 4 cut(s) 50, 156, 246, 488
Hsp92II CATG 6 cut(s) 37, 223, 274, 366, 374, 542
HspAI GCGC 3 cut(s) 216, 223, 266
Kzo9I GATC 2 cut(s) 139, 292
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 5 cut(s) 66, 107, 401, 404, 449
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 108, 347
MalI GATC 2 cut(s) 141, 294
MboI GATC 2 cut(s) 139, 292
MboII GAAGA 1 cut(s) 31
MflI RGATCY 1 cut(s) 292
MluCI AATT 3 cut(s) 206, 382, 457
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 8 cut(s) 140, 206, 212, 262, 430, 498, 510, 513
MslI CAYNNNNRTG 1 cut(s) 32
MspI CCGG 1 cut(s) 53
Mva1269I GAATGC 2 cut(s) 447, 508
MwoI GCNNNNNNNGC 1 cut(s) 222
NdeII GATC 2 cut(s) 139, 292
NheI GCTAGC 1 cut(s) 59
NlaIII CATG 6 cut(s) 37, 223, 274, 366, 374, 542
NmuCI GTSAC 1 cut(s) 347
NsbI TGCGCA 1 cut(s) 224
NspI RCATGY 1 cut(s) 374
PagI TCATGA 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 14
PctI GAATGC 2 cut(s) 447, 508
PkrI GCNGC 3 cut(s) 127, 215, 430
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PspEI GGTNACC 1 cut(s) 347
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 32
SalI GTCGAC 2 cut(s) 115, 185
SapI GCTCTTC 1 cut(s) 14
SatI GCNGC 3 cut(s) 126, 214, 429
Sau3AI GATC 2 cut(s) 139, 292
SchI GAGTC 1 cut(s) 47
SetI ASST 9 cut(s) 61, 71, 110, 159, 176, 243, 254, 303, 468
SfaNI GCATC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 3 cut(s) 206, 382, 457
SsiI CCGC 3 cut(s) 126, 352, 429
SspMI CTAG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 114, 286
TaiI ACGT 1 cut(s) 159
TaqI TCGA 4 cut(s) 116, 186, 204, 258
TaqII GACCGA 1 cut(s) 20
TasI AATT 3 cut(s) 206, 382, 457
TauI GCSGC 2 cut(s) 128, 431
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 1 cut(s) 213
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 3 cut(s) 287, 351, 527
TspRI CASTG 1 cut(s) 235
XapI RAATTY 2 cut(s) 206, 382
XceI RCATGY 1 cut(s) 374
XmiI GTMKAC 2 cut(s) 116, 186
XspI CTAG 1 cut(s) 60
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.