MD08G1006600.v1.1

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003844
Physical Location & Seq
Reverse (-)
537193 .. 538944
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1006600.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGCGACTCCGGTGAAGGTGTACGGACCACCGTTATCGACGGCGGTGTCAAGAGTGTTGGCCTGTCTCCATGAGAAAGGGGTGGACTATCAGCTCATTTCCGTCAACATGGCCAAAGGCGAGCACAAGAAGCCCGACTACCTCAAGATTCAGCCATTCGGACAAGTACCAGCTTTTGAAGACGAAGACATCTCCCTCTTCGAATCCAGAGCCATATGCCGGTACATATGTAACAAGTATGCAGATAAGGGAAACAAGGGTTTGTACGGAATGAATCCATTGGCGAAAGCCTCAATAGATCAGTGGCTGGAGGCCGAAGGGCAGAGCTTCAGCCCTCCGAGCTCAACGCTGGTGTTTCAGCTAGCATTTGCGCCGAGAATGAAGCTGAAGCAAGACGAGGGAGTGATAAGGCAGAACGAAGAGAAGCTTGCCAAGGTGCTGGATGTGTACGAAAAGAGGCTGGGGGGGAGCCGGTTCTTGGCGGGGGATGAATTCTCGCTGGCTGATCTGTCGCACTTGCCCAACACGCAGTACCTGGTGAATGCCACGGACAGGGGAGAGCTGATTACTTCGAGAGAGAATGTGGGAAGGTGGTGGTCTGAGATATCGAGCCGGGAGTCGTGGCAGAAGGTGGTTGAGATGCAAAAGCCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_N

Protein Analysis

219

Amino Acids

24.56

Weight (kDa)

6.84

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 4 - 76 7e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 8 - 83 3.9e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 15 - 78 4.1e-14 Glutathione S-transferase, N-terminal domain
GST_C PF00043 119 - 206 5e-12 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 136 - 198 2.4e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
AciI CCGC 2 cut(s) 44, 482
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 2 cut(s) 313, 407
AfaI GTAC 6 cut(s) 23, 168, 224, 266, 449, 533
AfiI CCNNNNNNNGG 3 cut(s) 219, 478, 552
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 8 cut(s) 94, 173, 327, 342, 361, 385, 427, 562
AluI AGCT 8 cut(s) 94, 173, 327, 342, 361, 385, 427, 562
Alw21I GWGCWC 2 cut(s) 126, 344
Alw26I GTCTC 1 cut(s) 71
AlwNI CAGNNNCTG 2 cut(s) 307, 535
AoxI GGCC 3 cut(s) 60, 111, 312
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 25, 550
AsuII TTCGAA 1 cut(s) 201
AsuNHI GCTAGC 1 cut(s) 361
AvaII GGWCC 1 cut(s) 26
BaeI ACNNNNGTAYC 2 cut(s) 214, 247
BalI TGGCCA 1 cut(s) 113
BanII GRGCYC 1 cut(s) 344
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BbsI GAAGAC 2 cut(s) 186, 192
Bbv12I GWGCWC 2 cut(s) 126, 344
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 536
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 1 cut(s) 362
Bme1390I CCNGG 2 cut(s) 536, 614
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 470
BmrFI CCNGG 2 cut(s) 536, 614
BmsI GCATC 1 cut(s) 630
BmtI GCTAGC 1 cut(s) 365
BpiI GAAGAC 2 cut(s) 186, 192
BpmI CTGGAG 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 432, 546
BsaWI WCCGGW 1 cut(s) 10
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 478, 552
Bse118I RCCGGY 2 cut(s) 219, 471
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 2 cut(s) 432, 546
BseGI GGATG 2 cut(s) 448, 493
BseLI CCNNNNNNNGG 3 cut(s) 219, 478, 552
BseMII CTCAG 1 cut(s) 591
BseYI CCCAGC 1 cut(s) 460
BshFI GGCC 3 cut(s) 62, 113, 314
BsiHKAI GWGCWC 2 cut(s) 126, 344
BsiSI CCGG 4 cut(s) 11, 220, 472, 613
BslI CCNNNNNNNGG 3 cut(s) 219, 478, 552
BsmAI GTCTC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 3 cut(s) 62, 113, 314
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 126, 344
Bsp143I GATC 2 cut(s) 298, 505
BspACI CCGC 2 cut(s) 44, 482
BspANI GGCC 3 cut(s) 62, 113, 314
BspCNI CTCAG 1 cut(s) 592
BspLI GGNNCC 1 cut(s) 470
BspOI GCTAGC 1 cut(s) 365
BspT104I TTCGAA 1 cut(s) 201
BsrFI RCCGGY 2 cut(s) 219, 471
BssAI RCCGGY 2 cut(s) 219, 471
BssECI CCNNGG 2 cut(s) 432, 546
BssMI GATC 2 cut(s) 298, 505
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 2 cut(s) 203, 414
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 122, 363, 429, 501
BstDEI CTNAG 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 2 cut(s) 448, 493
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 2 cut(s) 301, 508
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 2 cut(s) 298, 505
BstMWI GCNNNNNNNGC 3 cut(s) 130, 339, 526
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 534, 612
BstV2I GAAGAC 2 cut(s) 186, 192
BstXI CCANNNNNNTGG 1 cut(s) 439
BsuRI GGCC 3 cut(s) 62, 113, 314
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 2 cut(s) 448, 493
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 4 cut(s) 122, 363, 429, 501
CaiI CAGNNNCTG 2 cut(s) 307, 535
CfoI GCGC 1 cut(s) 373
Cfr10I RCCGGY 2 cut(s) 219, 471
Cfr13I GGNCC 1 cut(s) 26
CsiI ACCWGGT 1 cut(s) 534
Csp6I GTAC 6 cut(s) 22, 167, 223, 265, 448, 532
CviAII CATG 2 cut(s) 71, 109
CviQI GTAC 6 cut(s) 22, 167, 223, 265, 448, 532
DdeI CTNAG 1 cut(s) 600
DpnI GATC 2 cut(s) 300, 507
DpnII GATC 2 cut(s) 298, 505
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 2 cut(s) 203, 414
EarI CTCTTC 2 cut(s) 203, 414
Ecl136II GAGCTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 344
Eco32I GATATC 1 cut(s) 606
Eco47I GGWCC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 313, 407
EcoICRI GAGCTC 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 606
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 2 cut(s) 74, 112
FaiI YATR 7 cut(s) 72, 110, 215, 217, 227, 229, 240
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 2 cut(s) 70, 108
FauI CCCGC 1 cut(s) 475
FauNDI CATATG 2 cut(s) 215, 227
FokI GGATG 2 cut(s) 455, 500
FriOI GRGCYC 1 cut(s) 344
FspBI CTAG 1 cut(s) 362
GlaI GCGC 1 cut(s) 372
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 1 cut(s) 329
HaeIII GGCC 3 cut(s) 62, 113, 314
HapII CCGG 4 cut(s) 11, 220, 472, 613
HhaI GCGC 1 cut(s) 373
Hin1II CATG 2 cut(s) 74, 112
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 5 cut(s) 7, 148, 203, 274, 617
HpaII CCGG 4 cut(s) 11, 220, 472, 613
HphI GGTGA 2 cut(s) 25, 550
Hpy166II GTNNAC 4 cut(s) 22, 85, 106, 448
Hpy188I TCNGA 3 cut(s) 161, 339, 601
Hpy188III TCNNGA 4 cut(s) 51, 145, 207, 573
Hpy8I GTNNAC 4 cut(s) 22, 85, 106, 448
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 4 cut(s) 10, 311, 582, 622
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 2 cut(s) 242, 643
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 339, 526
HpyF3I CTNAG 1 cut(s) 600
Hsp92II CATG 2 cut(s) 74, 112
HspAI GCGC 1 cut(s) 371
Kzo9I GATC 2 cut(s) 298, 505
LmnI GCTCC 1 cut(s) 468
LweI GCATC 1 cut(s) 630
MabI ACCWGGT 1 cut(s) 534
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 230
MalI GATC 2 cut(s) 300, 507
MboI GATC 2 cut(s) 298, 505
MboII GAAGA 4 cut(s) 190, 191, 197, 431
MhlI GDGCHC 2 cut(s) 126, 344
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 1 cut(s) 491
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 1 cut(s) 626
MnlI CCTC 7 cut(s) 152, 206, 301, 304, 345, 391, 450
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
MseI TTAA 1 cut(s) 655
Msp20I TGGCCA 1 cut(s) 113
MspI CCGG 4 cut(s) 11, 220, 472, 613
MspR9I CCNGG 2 cut(s) 536, 614
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 3 cut(s) 130, 339, 526
NciI CCSGG 1 cut(s) 614
NdeI CATATG 2 cut(s) 215, 227
NdeII GATC 2 cut(s) 298, 505
NheI GCTAGC 1 cut(s) 361
NlaIII CATG 2 cut(s) 74, 112
NlaIV GGNNCC 1 cut(s) 470
NmeAIII GCCGAG 1 cut(s) 399
NspV TTCGAA 1 cut(s) 201
PctI GAATGC 1 cut(s) 547
PfeI GAWTC 3 cut(s) 148, 203, 274
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
Psp124BI GAGCTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 534
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 470
PspPI GGNCC 1 cut(s) 26
PstNI CAGNNNCTG 2 cut(s) 307, 535
RsaI GTAC 6 cut(s) 23, 168, 224, 266, 449, 533
RsaNI GTAC 6 cut(s) 22, 167, 223, 265, 448, 532
SacI GAGCTC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 655
Sau3AI GATC 2 cut(s) 298, 505
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 2 cut(s) 536, 614
SduI GDGCHC 2 cut(s) 126, 344
SexAI ACCWGGT 1 cut(s) 534
SfaNI GCATC 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 1 cut(s) 491
SsiI CCGC 2 cut(s) 44, 482
SspMI CTAG 1 cut(s) 362
SstI GAGCTC 1 cut(s) 344
StyD4I CCNGG 2 cut(s) 534, 612
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 4 cut(s) 38, 201, 572, 608
TasI AATT 1 cut(s) 491
TfiI GAWTC 3 cut(s) 148, 203, 274
Tru1I TTAA 1 cut(s) 655
Tru9I TTAA 1 cut(s) 655
TscAI CASTG 1 cut(s) 308
TspDTI ATGAA 3 cut(s) 287, 395, 504
TspGWI ACGGA 4 cut(s) 39, 91, 282, 563
TspRI CASTG 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 1 cut(s) 491
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.