MD08G1011900.v1.1

thaumatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006511
Physical Location & Seq
Reverse (-)
904633 .. 908383
3751 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1011900.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGCTCGATTACCATCATTGTTAATTTCTCTGTTAACCATCCTCGGCCTACAAACATCAGGTGTTGTTTCAAGGACATTCACAATGGAGAACAAGTGCGACTACACAGTGTGGCCGGGCATTCTCACCAACGCCGGCGTCTCACCTCTGCCGACCACCGGCTTCGCCCTCGAAAAGGGCGAAACGAAAACTATCTCCGCACCAGCCTCGTGGGGCGGCCGCTTCTGGGGCCGAACTCTCTGCTCCCACGACTCCGCCGGTAAATTATCCTGCCTCACCGCCGACTGCGGCTCAGGAAAATTAGAATGCTCAGGCAACGGAGCCGCCCCGCCCGCCACTGTCGCTGAATTCACACTCGACGGCGCCGGCGGGCTTGATTTCTTCGACGTCAGCCTCGTAGACGGGTACAACCTCCCTATGCTGGTGGTCCCCAATGGCGGAACCGGGACCAAGTGTACGAACACCGGCTGTGTTACCGACCTCAACGCCAGCTGCCCTTCCGAGCTCAAGGTCACCGGTGCCGACGGAGCTGAAGCAGTCGCGTGCAAGAGCGCGTGCGAGGCGTTCGGCGAGCAGCAGTACTGTTGTAGCGGCGCCTACGGTTCTCCCGACACTTGCAAGCCCTCTCAATACTCTCAGATTTTCAAGAGCGCATGCCCGCGCGCATACAGTTACGCTTACGACGACAAGACCAGCACCTTCACATGCGCCGGTGGCGACTACACCATCATCTTCTGCCCATCGCGTAGCACCAGCCAGAAAGCATCACAGGATAACACCCAGCCGCAGCAGACAACTACACCGCCGTCGACAACCACCTCAACCTCGCTGCTGGAGATAAACAACACAATGGAGCACAAATGCGGCTCAAACGATGATTCATTTTCGGCCGTGTCCATTCACACGTGGACCCATGTGTGTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

309

Amino Acids

32.2

Weight (kDa)

4.96

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 31 - 247 4.8e-85 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccB1I GGYRCC 3 cut(s) 362, 518, 593
AccI GTMKAC 2 cut(s) 399, 809
AccII CGCG 5 cut(s) 542, 554, 661, 663, 745
AcoI YGGCCR 3 cut(s) 113, 217, 888
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 552
AcvI CACGTG 1 cut(s) 906
AcyI GRCGYC 4 cut(s) 138, 363, 387, 594
AdeI CACNNNGTG 1 cut(s) 111
AfaI GTAC 3 cut(s) 407, 457, 581
AfiI CCNNNNNNNGG 5 cut(s) 158, 175, 226, 421, 437
AflIII ACRYGT 1 cut(s) 903
AgeI ACCGGT 1 cut(s) 515
AgsI TTSAA 2 cut(s) 72, 646
AluBI AGCT 3 cut(s) 492, 505, 530
AluI AGCT 3 cut(s) 492, 505, 530
Alw21I GWGCWC 2 cut(s) 507, 858
Alw26I GTCTC 1 cut(s) 145
AoxI GGCC 5 cut(s) 46, 113, 217, 229, 888
ApeKI GCWGC 4 cut(s) 492, 574, 787, 829
ApoI RAATTY 1 cut(s) 347
AsiGI ACCGGT 1 cut(s) 515
AspLEI GCGC 7 cut(s) 365, 554, 596, 653, 663, 665, 710
AspS9I GGNCC 4 cut(s) 229, 427, 447, 909
AsuC2I CCSGG 2 cut(s) 117, 445
AsuHPI GGTGA 4 cut(s) 118, 135, 268, 505
AvaII GGWCC 3 cut(s) 427, 447, 909
BanI GGYRCC 3 cut(s) 362, 518, 593
BanII GRGCYC 1 cut(s) 507
BauI CACGAG 1 cut(s) 208
BbrPI CACGTG 1 cut(s) 906
Bbv12I GWGCWC 2 cut(s) 507, 858
BbvI GCAGC 4 cut(s) 479, 586, 799, 816
BccI CCATC 4 cut(s) 22, 47, 734, 748
BceAI ACGGC 3 cut(s) 376, 790, 875
BcgI CGANNNNNNTGC 4 cut(s) 189, 222, 223, 256
BcnI CCSGG 2 cut(s) 117, 445
BcoDI GTCTC 1 cut(s) 145
BfoI RGCGCY 2 cut(s) 366, 597
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 2 cut(s) 117, 445
Bme18I GGWCC 3 cut(s) 427, 447, 909
BmgT120I GGNCC 4 cut(s) 229, 427, 447, 909
BmiI GGNNCC 9 cut(s) 230, 322, 364, 429, 442, 448, 520, 595, 911
BmrFI CCNGG 2 cut(s) 117, 445
BmsI GCATC 1 cut(s) 773
BpmI CTGGAG 1 cut(s) 854
Bpu10I CCTNAGC 2 cut(s) 292, 310
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 117, 445
BsaAI YACGTR 1 cut(s) 906
BsaBI GATNNNNATC 1 cut(s) 13
BsaHI GRCGYC 4 cut(s) 138, 363, 387, 594
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 515
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 175, 226, 421, 437
Bse118I RCCGGY 7 cut(s) 134, 158, 257, 365, 464, 515, 710
Bse8I GATNNNNATC 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 39
BseJI GATNNNNATC 1 cut(s) 13
BseLI CCNNNNNNNGG 5 cut(s) 158, 175, 226, 421, 437
BseMII CTCAG 3 cut(s) 306, 324, 650
BsePI GCGCGC 1 cut(s) 661
BseX3I CGGCCG 2 cut(s) 217, 888
BseXI GCAGC 4 cut(s) 479, 586, 799, 816
BseYI CCCAGC 1 cut(s) 780
Bsh1236I CGCG 5 cut(s) 542, 554, 661, 663, 745
Bsh1285I CGRYCG 2 cut(s) 220, 891
BshFI GGCC 5 cut(s) 48, 115, 219, 231, 890
BshNI GGYRCC 3 cut(s) 362, 518, 593
BshTI ACCGGT 1 cut(s) 515
BsiEI CGRYCG 2 cut(s) 220, 891
BsiHKAI GWGCWC 2 cut(s) 507, 858
BsiSI CCGG 9 cut(s) 116, 135, 159, 258, 366, 444, 465, 516, 711
BslFI GGGAC 2 cut(s) 413, 460
BslI CCNNNNNNNGG 5 cut(s) 158, 175, 226, 421, 437
BsmAI GTCTC 1 cut(s) 145
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 413, 460
BsmI GAATGC 2 cut(s) 120, 311
BsnI GGCC 5 cut(s) 48, 115, 219, 231, 890
Bsp1286I GDGCHC 2 cut(s) 507, 858
BspANI GGCC 5 cut(s) 48, 115, 219, 231, 890
BspCNI CTCAG 3 cut(s) 305, 323, 649
BspFNI CGCG 5 cut(s) 542, 554, 661, 663, 745
BspLI GGNNCC 9 cut(s) 230, 322, 364, 429, 442, 448, 520, 595, 911
BspT107I GGYRCC 3 cut(s) 362, 518, 593
BsrFI RCCGGY 7 cut(s) 134, 158, 257, 365, 464, 515, 710
BssAI RCCGGY 7 cut(s) 134, 158, 257, 365, 464, 515, 710
BssECI CCNNGG 1 cut(s) 43
BssHII GCGCGC 1 cut(s) 661
BssNI GRCGYC 4 cut(s) 138, 363, 387, 594
BssSI CACGAG 1 cut(s) 208
Bst2BI CACGAG 1 cut(s) 208
Bst4CI ACNGT 5 cut(s) 109, 340, 584, 602, 671
BstACI GRCGYC 4 cut(s) 138, 363, 387, 594
BstBAI YACGTR 1 cut(s) 906
BstDEI CTNAG 3 cut(s) 292, 310, 636
BstEII GGTNACC 1 cut(s) 511
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 39
BstFNI CGCG 5 cut(s) 542, 554, 661, 663, 745
BstH2I RGCGCY 2 cut(s) 366, 597
BstHHI GCGC 7 cut(s) 365, 554, 596, 653, 663, 665, 710
BstMAI GTCTC 1 cut(s) 145
BstMCI CGRYCG 2 cut(s) 220, 891
BstMWI GCNNNNNNNGC 6 cut(s) 228, 332, 341, 527, 560, 714
BstNSI RCATGY 2 cut(s) 657, 708
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 115, 443
BstUI CGCG 5 cut(s) 542, 554, 661, 663, 745
BstV1I GCAGC 4 cut(s) 479, 586, 799, 816
BstXI CCANNNNNNTGG 1 cut(s) 210
BstZI CGGCCG 2 cut(s) 217, 888
BsuRI GGCC 5 cut(s) 48, 115, 219, 231, 890
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 114, 336
CciNI GCGGCCGC 1 cut(s) 217
CfoI GCGC 7 cut(s) 365, 554, 596, 653, 663, 665, 710
Cfr10I RCCGGY 7 cut(s) 134, 158, 257, 365, 464, 515, 710
Cfr13I GGNCC 4 cut(s) 229, 427, 447, 909
CseI GACGC 1 cut(s) 127
Csp6I GTAC 3 cut(s) 406, 456, 580
CspAI ACCGGT 1 cut(s) 515
CviAII CATG 3 cut(s) 654, 705, 914
CviQI GTAC 3 cut(s) 406, 456, 580
DdeI CTNAG 3 cut(s) 292, 310, 636
DinI GGCGCC 2 cut(s) 364, 595
DraIII CACNNNGTG 1 cut(s) 111
EaeI YGGCCR 3 cut(s) 113, 217, 888
EagI CGGCCG 2 cut(s) 217, 888
EciI GGCGGA 2 cut(s) 244, 453
Ecl136II GAGCTC 1 cut(s) 505
EclXI CGGCCG 2 cut(s) 217, 888
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 3 cut(s) 427, 447, 909
Eco52I CGGCCG 2 cut(s) 217, 888
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 552
Eco72I CACGTG 1 cut(s) 906
Eco91I GGTNACC 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 505
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 507
EgeI GGCGCC 2 cut(s) 364, 595
EheI GGCGCC 2 cut(s) 364, 595
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 3 cut(s) 657, 708, 917
FaiI YATR 6 cut(s) 419, 655, 667, 706, 915, 925
FaqI GGGAC 2 cut(s) 413, 460
FatI CATG 3 cut(s) 653, 704, 913
FauI CCCGC 4 cut(s) 336, 340, 362, 666
FblI GTMKAC 2 cut(s) 399, 809
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 507
GlaI GCGC 7 cut(s) 364, 553, 595, 652, 662, 664, 709
GsaI CCCAGC 1 cut(s) 784
GsuI CTGGAG 1 cut(s) 854
HaeII RGCGCY 2 cut(s) 366, 597
HaeIII GGCC 5 cut(s) 48, 115, 219, 231, 890
HapII CCGG 9 cut(s) 116, 135, 159, 258, 366, 444, 465, 516, 711
HgaI GACGC 1 cut(s) 127
HhaI GCGC 7 cut(s) 365, 554, 596, 653, 663, 665, 710
Hin1I GRCGYC 4 cut(s) 138, 363, 387, 594
Hin1II CATG 3 cut(s) 657, 708, 917
Hin6I GCGC 7 cut(s) 363, 552, 594, 651, 661, 663, 708
HinP1I GCGC 7 cut(s) 363, 552, 594, 651, 661, 663, 708
HincII GTYRAC 2 cut(s) 36, 810
HindII GTYRAC 2 cut(s) 36, 810
HinfI GANTC 2 cut(s) 251, 878
HpaI GTTAAC 1 cut(s) 36
HpaII CCGG 9 cut(s) 116, 135, 159, 258, 366, 444, 465, 516, 711
HphI GGTGA 4 cut(s) 118, 135, 268, 505
Hpy166II GTNNAC 5 cut(s) 36, 400, 456, 810, 909
Hpy188I TCNGA 2 cut(s) 502, 639
Hpy188III TCNNGA 3 cut(s) 294, 608, 646
Hpy8I GTNNAC 5 cut(s) 36, 400, 456, 810, 909
Hpy99I CGWCG 5 cut(s) 362, 389, 527, 686, 811
HpyAV CCTTC 2 cut(s) 507, 709
HpyCH4III ACNGT 5 cut(s) 109, 340, 584, 602, 671
HpyCH4IV ACGT 2 cut(s) 387, 905
HpyCH4V TGCA 2 cut(s) 546, 618
HpyF10VI GCNNNNNNNGC 6 cut(s) 228, 332, 341, 527, 560, 714
HpyF3I CTNAG 3 cut(s) 292, 310, 636
HpySE526I ACGT 2 cut(s) 387, 905
Hsp92I GRCGYC 4 cut(s) 138, 363, 387, 594
Hsp92II CATG 3 cut(s) 657, 708, 917
HspAI GCGC 7 cut(s) 363, 552, 594, 651, 661, 663, 708
KasI GGCGCC 2 cut(s) 362, 593
KroI GCCGGC 2 cut(s) 134, 365
KroNI GCCGGC 2 cut(s) 136, 367
KspAI GTTAAC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 248, 320, 527, 853
Lsp1109I GCAGC 4 cut(s) 479, 586, 799, 816
LweI GCATC 1 cut(s) 773
MaeII ACGT 2 cut(s) 387, 905
MaeIII GTNAC 3 cut(s) 472, 511, 671
MboII GAAGA 2 cut(s) 373, 724
MhlI GDGCHC 2 cut(s) 507, 858
MluCI AATT 4 cut(s) 24, 263, 299, 347
Mly113I GGCGCC 2 cut(s) 363, 594
MlyI GAGTC 1 cut(s) 245
MreI CGCCGGCG 2 cut(s) 134, 365
MroNI GCCGGC 2 cut(s) 134, 365
MseI TTAA 2 cut(s) 23, 35
MspA1I CMGCKG 1 cut(s) 492
MspI CCGG 9 cut(s) 116, 135, 159, 258, 366, 444, 465, 516, 711
MspR9I CCNGG 2 cut(s) 117, 445
Mva1269I GAATGC 2 cut(s) 120, 311
MvnI CGCG 5 cut(s) 542, 554, 661, 663, 745
MwoI GCNNNNNNNGC 6 cut(s) 228, 332, 341, 527, 560, 714
NaeI GCCGGC 2 cut(s) 136, 367
NarI GGCGCC 2 cut(s) 363, 594
NciI CCSGG 2 cut(s) 117, 445
NgoMIV GCCGGC 2 cut(s) 134, 365
NlaIII CATG 3 cut(s) 657, 708, 917
NlaIV GGNNCC 9 cut(s) 230, 322, 364, 429, 442, 448, 520, 595, 911
NmeAIII GCCGAG 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 511
NotI GCGGCCGC 1 cut(s) 217
NspI RCATGY 2 cut(s) 657, 708
PaeI GCATGC 1 cut(s) 657
PauI GCGCGC 1 cut(s) 661
PcsI WCGNNNNNNNCGW 4 cut(s) 177, 393, 606, 681
PctI GAATGC 2 cut(s) 120, 311
PdiI GCCGGC 2 cut(s) 136, 367
PfeI GAWTC 1 cut(s) 878
PinAI ACCGGT 1 cut(s) 515
PleI GAGTC 1 cut(s) 245
PluTI GGCGCC 2 cut(s) 366, 597
PmaCI CACGTG 1 cut(s) 906
PmlI CACGTG 1 cut(s) 906
PpsI GAGTC 1 cut(s) 245
Ppu21I YACGTR 1 cut(s) 906
Psp124BI GAGCTC 1 cut(s) 507
PspCI CACGTG 1 cut(s) 906
PspEI GGTNACC 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 780
PspN4I GGNNCC 9 cut(s) 230, 322, 364, 429, 442, 448, 520, 595, 911
PspPI GGNCC 4 cut(s) 229, 427, 447, 909
PteI GCGCGC 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 492
RsaI GTAC 3 cut(s) 407, 457, 581
RsaNI GTAC 3 cut(s) 406, 456, 580
SacI GAGCTC 1 cut(s) 507
SalI GTCGAC 1 cut(s) 808
SaqAI TTAA 2 cut(s) 23, 35
Sau96I GGNCC 4 cut(s) 229, 427, 447, 909
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 117, 445
SduI GDGCHC 2 cut(s) 507, 858
SfaNI GCATC 1 cut(s) 773
SfoI GGCGCC 2 cut(s) 364, 595
SgrAI CRCCGGYG 4 cut(s) 134, 365, 515, 710
SinI GGWCC 3 cut(s) 427, 447, 909
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SphI GCATGC 1 cut(s) 657
Sse9I AATT 4 cut(s) 24, 263, 299, 347
SspDI GGCGCC 2 cut(s) 362, 593
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 115, 443
TaaI ACNGT 5 cut(s) 109, 340, 584, 602, 671
TaiI ACGT 2 cut(s) 390, 908
TaqI TCGA 5 cut(s) 7, 171, 357, 384, 809
TasI AATT 4 cut(s) 24, 263, 299, 347
TatI WGTACW 1 cut(s) 579
TauI GCSGC 7 cut(s) 219, 222, 291, 326, 594, 787, 867
TfiI GAWTC 1 cut(s) 878
Tru1I TTAA 2 cut(s) 23, 35
Tru9I TTAA 2 cut(s) 23, 35
TscAI CASTG 2 cut(s) 114, 343
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 4 cut(s) 492, 574, 787, 829
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 1 cut(s) 870
TspGWI ACGGA 2 cut(s) 333, 540
TspRI CASTG 2 cut(s) 114, 343
VpaK11BI GGWCC 3 cut(s) 427, 447, 909
XagI CCTNNNNNAGG 1 cut(s) 173
XapI RAATTY 1 cut(s) 347
XceI RCATGY 2 cut(s) 657, 708
XmiI GTMKAC 2 cut(s) 399, 809
ZraI GACGTC 1 cut(s) 388
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.