MD08G1030000.v1.1

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008298
Physical Location & Seq
Forward (+)
2171370 .. 2172137
768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1030000.v1.1.491

Sequence Viewer

Length: 678 bp
ATGCTGTGCAGGTCTAATTTTCTGGGTTCCAAGTTTACCGTTTATGACAGTCAGCCTCCACAAGGCTCAGCAATCCAAACAAATTATTGGTCACACCGAAAGATGAATTCAAAGACGGTATCTCCAAGGATACCTGCTGGTAGCTATAGTGTTGCTACCGTATGTTATGAGCTCAATATCCTCCGAACAAGGGGACCAAGGAGAATGCAGTGCATCATGCAATCAATCCCCGTGTCAGCAGTTCAAGAAGGGGGCGTTGCTCCTACTCCAACAGAGTTAGCGACCTGCCTTCCTCCAAAATGCTGTCCAATGCATGTTTCAAAGGGATCGAAGCAGCTTGCCAACTTGTGCTCTAGTGTCCCTACAACGGATTTGGAACAAGGCACAAAAGATCCACTCATTTTGAAAAATAAAGCTCCTAGATGGCATGAGCAGCTGCAGTGTTGGTGTCTGAATTTTAAAGGACGCGTTACCGTGGCCTCCGTGAAGAATTTCCAGCTGGTGGCCGGTGTAGAGCCATGCCATAATGTTCCTTTGGCTGAACAAGAGAGAGTAATACTGCAATTTGGGAAGATCGGTAAAGACATTTTCACAATGGATTATCGCTACCCTCTCTCTGCTTTCCAAGCCTTTGCAGTTTGCTTGAGCAGCTTTGACACAAAACCAGCATGCGAATGA

Protein Analysis

226

Amino Acids

24.95

Weight (kDa)

9.11

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 4 - 220 3.7e-72 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 142, 293
AccB7I CCANNNNNTGG 1 cut(s) 502
AccII CGCG 1 cut(s) 468
AclWI GGATC 2 cut(s) 334, 386
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 3 cut(s) 106, 454, 490
AfiI CCNNNNNNNGG 4 cut(s) 62, 190, 367, 502
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 4 cut(s) 111, 245, 321, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 301, 333
AluBI AGCT 7 cut(s) 144, 172, 337, 416, 436, 499, 651
AluI AGCT 7 cut(s) 144, 172, 337, 416, 436, 499, 651
Alw21I GWGCWC 2 cut(s) 174, 353
AlwI GGATC 2 cut(s) 334, 386
AoxI GGCC 2 cut(s) 477, 504
ApeKI GCWGC 4 cut(s) 334, 433, 436, 648
ApoI RAATTY 3 cut(s) 106, 454, 490
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 194
AvaII GGWCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 2 cut(s) 174, 353
BbvI GCAGC 4 cut(s) 346, 423, 445, 660
BccI CCATC 1 cut(s) 417
BciVI GTATCC 1 cut(s) 123
BfaI CTAG 2 cut(s) 354, 420
BfmI CTRYAG 2 cut(s) 145, 437
BfuAI ACCTGC 2 cut(s) 142, 293
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 4 cut(s) 335, 434, 437, 649
BlpI GCTNAGC 1 cut(s) 67
BlsI GCNGC 4 cut(s) 336, 435, 438, 650
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 2 cut(s) 28, 195
BmsI GCATC 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 664
BsaJI CCNNGG 3 cut(s) 125, 197, 474
BsaXI ACNNNNNCTCC 2 cut(s) 106, 136
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 190, 367, 502
Bse118I RCCGGY 1 cut(s) 506
BseDI CCNNGG 3 cut(s) 125, 197, 474
BseLI CCNNNNNNNGG 4 cut(s) 62, 190, 367, 502
BseMII CTCAG 1 cut(s) 81
BseXI GCAGC 4 cut(s) 346, 423, 445, 660
BsgI GTGCAG 1 cut(s) 28
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 2 cut(s) 479, 506
BsiHKAI GWGCWC 2 cut(s) 174, 353
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 2 cut(s) 207, 344
BslI CCNNNNNNNGG 4 cut(s) 62, 190, 367, 502
BsmFI GGGAC 2 cut(s) 207, 344
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 2 cut(s) 479, 506
Bsp1286I GDGCHC 2 cut(s) 174, 353
Bsp143I GATC 3 cut(s) 326, 391, 573
Bsp1720I GCTNAGC 1 cut(s) 67
BspANI GGCC 2 cut(s) 479, 506
BspCNI CTCAG 1 cut(s) 80
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 28, 195
BspMAI CTGCAG 1 cut(s) 441
BspMI ACCTGC 2 cut(s) 142, 293
BspPI GGATC 2 cut(s) 334, 386
BsrFI RCCGGY 1 cut(s) 506
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 125, 197, 474
BssMI GATC 3 cut(s) 326, 391, 573
BssT1I CCWWGG 2 cut(s) 125, 197
Bst4CI ACNGT 5 cut(s) 40, 50, 118, 160, 475
BstC8I GCNNGC 2 cut(s) 339, 670
BstDEI CTNAG 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 474
BstENI CCTNNNNNAGG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 468
BstKTI GATC 3 cut(s) 329, 394, 576
BstMBI GATC 3 cut(s) 326, 391, 573
BstMWI GCNNNNNNNGC 3 cut(s) 433, 626, 648
BstNSI RCATGY 2 cut(s) 317, 672
BstSFI CTRYAG 2 cut(s) 145, 437
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 4 cut(s) 346, 423, 445, 660
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 2 cut(s) 479, 506
BtgI CCRYGG 1 cut(s) 474
BtsI GCAGTG 2 cut(s) 215, 446
BtsIMutI CAGTG 2 cut(s) 215, 446
BveI ACCTGC 2 cut(s) 142, 293
Cac8I GCNNGC 2 cut(s) 339, 670
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 1 cut(s) 194
CseI GACGC 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 5 cut(s) 217, 314, 428, 519, 669
DdeI CTNAG 1 cut(s) 67
DpnI GATC 3 cut(s) 328, 393, 575
DpnII GATC 3 cut(s) 326, 391, 573
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 504
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 125, 197
Eco24I GRGCYC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 125, 197
EcoT22I ATGCAT 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 125, 197
FaeI CATG 5 cut(s) 220, 317, 431, 522, 672
FaqI GGGAC 2 cut(s) 207, 344
FatI CATG 5 cut(s) 216, 313, 427, 518, 668
Fnu4HI GCNGC 4 cut(s) 335, 434, 437, 649
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 4 cut(s) 335, 434, 437, 649
FspBI CTAG 2 cut(s) 354, 420
GluI GCNGC 4 cut(s) 335, 434, 437, 649
HaeIII GGCC 2 cut(s) 479, 506
HapII CCGG 1 cut(s) 507
HgaI GACGC 1 cut(s) 474
Hin1II CATG 5 cut(s) 220, 317, 431, 522, 672
HpaII CCGG 1 cut(s) 507
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 2 cut(s) 185, 453
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 1 cut(s) 36
HpyAV CCTTC 2 cut(s) 242, 299
HpyCH4III ACNGT 5 cut(s) 40, 50, 118, 160, 475
HpyCH4V TGCA 8 cut(s) 9, 208, 213, 220, 313, 439, 562, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 433, 626, 648
HpyF3I CTNAG 1 cut(s) 67
Hsp92II CATG 5 cut(s) 220, 317, 431, 522, 672
Kzo9I GATC 3 cut(s) 326, 391, 573
LmnI GCTCC 2 cut(s) 265, 421
LpnPI CCDG 7 cut(s) 8, 123, 147, 298, 485, 509, 520
Lsp1109I GCAGC 4 cut(s) 346, 423, 445, 660
LweI GCATC 1 cut(s) 222
MaeI CTAG 2 cut(s) 354, 420
MaeIII GTNAC 2 cut(s) 90, 469
MalI GATC 3 cut(s) 328, 393, 575
MboI GATC 3 cut(s) 326, 391, 573
MboII GAAGA 2 cut(s) 499, 583
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 2 cut(s) 174, 353
MluCI AATT 6 cut(s) 16, 82, 106, 454, 490, 563
MluI ACGCGT 1 cut(s) 466
MmeI TCCRAC 1 cut(s) 293
MnlI CCTC 5 cut(s) 66, 191, 303, 490, 621
Mph1103I ATGCAT 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 491
MseI TTAA 1 cut(s) 459
MslI CAYNNNNRTG 1 cut(s) 673
MspA1I CMGCKG 2 cut(s) 436, 499
MspI CCGG 1 cut(s) 507
Mva1269I GAATGC 1 cut(s) 210
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 3 cut(s) 433, 626, 648
NdeII GATC 3 cut(s) 326, 391, 573
NlaIII CATG 5 cut(s) 220, 317, 431, 522, 672
NlaIV GGNNCC 2 cut(s) 28, 195
NmuCI GTSAC 1 cut(s) 90
NsiI ATGCAT 1 cut(s) 315
NspI RCATGY 2 cut(s) 317, 672
PaeI GCATGC 1 cut(s) 672
PctI GAATGC 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 4 cut(s) 336, 435, 438, 650
Psp124BI GAGCTC 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 28, 195
PspPI GGNCC 1 cut(s) 194
PstI CTGCAG 1 cut(s) 441
PsuI RGATCY 1 cut(s) 391
PvuII CAGCTG 2 cut(s) 436, 499
RseI CAYNNNNRTG 1 cut(s) 673
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 4 cut(s) 335, 434, 437, 649
Sau3AI GATC 3 cut(s) 326, 391, 573
Sau96I GGNCC 1 cut(s) 194
SduI GDGCHC 2 cut(s) 174, 353
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 2 cut(s) 145, 437
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 673
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
SphI GCATGC 1 cut(s) 672
Sse9I AATT 6 cut(s) 16, 82, 106, 454, 490, 563
SspMI CTAG 2 cut(s) 354, 420
SstI GAGCTC 1 cut(s) 174
StyI CCWWGG 2 cut(s) 125, 197
TaaI ACNGT 5 cut(s) 40, 50, 118, 160, 475
TaqI TCGA 1 cut(s) 329
TasI AATT 6 cut(s) 16, 82, 106, 454, 490, 563
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 2 cut(s) 215, 446
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 4 cut(s) 334, 433, 436, 648
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 1 cut(s) 119
TspGWI ACGGA 2 cut(s) 383, 472
TspRI CASTG 2 cut(s) 215, 446
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 194
XagI CCTNNNNNAGG 1 cut(s) 60
XapI RAATTY 3 cut(s) 106, 454, 490
XceI RCATGY 2 cut(s) 317, 672
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 2 cut(s) 354, 420
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.