MD08G1031700.v1.1

methyltransferase PMT28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009069
Physical Location & Seq
Forward (+)
2272356 .. 2276232
3877 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1031700.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGCGGCGAAGGTGCCCTGCTGCGGGACGACGTTCTTTATTTACGTGCTGATAATCATAGGCCTGGTTTGCTTCGCCGGATTGATGGCCGGACTCACTCTCGGCCTCATGTCCCTCGGCCTCGTCGACCTCGAGGTCCTCATGAAGTCCGGCCAACCTCAGGATCGCAAGCACGCCGCTAAAATTTATCCGGTGGTGAAGAATCAGCACCTGTTACTTTGCACGCTTTTAATCGGAAACGCGTTAGCAATGGAGGCTCTTCCGGTGTTTTTGGATAGTCTTGTGCCTCCTTGGGCGGCTATTGTGATATCAGTCACTCTCATTCTGGTGTTTGGGGAGATATTGCCACAAGCAGTGTGCACTCGCTTTGGATTGACTGTTGGGGCGGCAATGGCCCCGTTTGTGCGTGTTCTTCTTGTTCTTTTCCTCCCCATTTCGTATCCCATTAGCAAGGTTTTGGATTATATGTTGGGTAAGGGACATTCTGCCCTTTTACGAAGAGCAGAACTAAAGACTTTTGTGGATTTTCATGGAAATGAGGCTGGGAAAGGCGGGGATTTAACACATGATGAGACTACCATCATTGCTGGGGCACTTGAATTGACTGAAAAGACTGCAAAAGATGCCATGACTCCCATATCAAATGCATTTTCCCTTGATCTGGATGCACCTCTTACTTTGGACACATTGAATGCAATAATGGCAATGGGTCATAGTAGAGTTCCAGTTTATGCTGGGAAGCCCGGAAATATAATTGGTCTAGTTCTGGTTAAGAATCTCTTGATGGTTGATCCAGAAGATGGAGTTACTTTAAGAAAACTGATGATAAGAAAAATTCCTCGGGTTTCGGAAGACATGCCTCTGTATGATATTCTAAATGAATTTCAGAAAGGTCACAGTCACATTGCTGTTGTCTATAAGGATATAAGTGGGAAAAAGGATGCACTAAAGAATGGCAAAGATGGTGAAACACCCGTGTTTAAGGATGACTGTAAGAAGCAAGCAGGCGAATCTGGGGCATCATTCAAGAAAGATCAAAAAGTGGGTTCAAACAATGCTCAGAGTGCTTTGGACAATGGTGACAGAGGTCAACAAGCGAAGAAAAGCCCATCAGCTACTCCCACTTTTAAGAAACGACACAGAGGCTGTTCACATTGCATTCTGGACATTGAGAACGGTCCCATTCCAGTTTTCCCAGACAATGAAGAGGTTGTAGGAGTAATTACCATGGAGGATGTCATTGAAGAACTTCTTCAGGAAGAGATATTGGACGAAACAGATGAGTATGTCAATATCCACAACAGGATTAAGGTCAATATGCATGCATCTGAGGAAAAGCCTTCTGACTCGAACTCAGCCGATCCTTCCAGAGAATGGTCATCTGTCACCAACGCCCCACTGCTAACATCAGCGTCAGTGCCATCAGATTTGGGTTCCACTCAACATCAAGACTCTGGTAAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21 Methyltransf_29

Protein Analysis

489

Amino Acids

52.76

Weight (kDa)

5.75

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 17 - 188 1.4e-33 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AbsI CCTCGAGG 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 13
AccB7I CCANNNNNTGG 2 cut(s) 800, 1374
AccI GTMKAC 1 cut(s) 126
AccII CGCG 1 cut(s) 242
AciI CCGC 6 cut(s) 5, 24, 177, 296, 386, 552
AclWI GGATC 3 cut(s) 171, 785, 1355
AcoI YGGCCR 2 cut(s) 87, 151
AcsI RAATTY 3 cut(s) 183, 834, 881
AcuI CTGAAG 1 cut(s) 1238
AfiI CCNNNNNNNGG 6 cut(s) 23, 24, 160, 661, 800, 1374
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 5 cut(s) 599, 691, 1027, 1050, 1244
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 1 cut(s) 1115
AluI AGCT 1 cut(s) 1115
Alw21I GWGCWC 1 cut(s) 362
Alw26I GTCTC 1 cut(s) 566
Alw44I GTGCAC 1 cut(s) 358
AlwI GGATC 3 cut(s) 171, 785, 1355
AlwNI CAGNNNCTG 2 cut(s) 211, 1146
Ama87I CYCGRG 2 cut(s) 131, 840
AoxI GGCC 6 cut(s) 61, 87, 103, 118, 151, 393
ApaLI GTGCAC 1 cut(s) 358
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 3 cut(s) 183, 834, 881
Asp700I GAANNNNTTC 2 cut(s) 1248, 1251
AspS9I GGNCC 3 cut(s) 136, 394, 1178
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 4 cut(s) 208, 977, 1091, 1378
AvaI CYCGRG 2 cut(s) 131, 840
AvaII GGWCC 2 cut(s) 136, 1178
AxyI CCTNAGG 1 cut(s) 159
BaeGI GKGCMC 3 cut(s) 18, 362, 595
BanI GGYRCC 1 cut(s) 13
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 8
BccI CCATC 7 cut(s) 79, 587, 778, 794, 956, 1117, 1429
BciT130I CCWGG 1 cut(s) 65
BciVI GTATCC 1 cut(s) 450
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 1 cut(s) 761
BfuI GTATCC 1 cut(s) 450
BisI GCNGC 5 cut(s) 6, 22, 177, 297, 387
BlsI GCNGC 5 cut(s) 7, 23, 178, 298, 388
Bme1390I CCNGG 2 cut(s) 65, 744
Bme18I GGWCC 2 cut(s) 136, 1178
BmeT110I CYCGRG 2 cut(s) 131, 840
BmgT120I GGNCC 3 cut(s) 136, 394, 1178
BmiI GGNNCC 4 cut(s) 15, 396, 1180, 1435
BmrFI CCNGG 2 cut(s) 65, 744
BmsI GCATC 5 cut(s) 613, 655, 931, 1028, 1334
BoxI GACNNNNGTC 1 cut(s) 1086
BpiI GAAGAC 1 cut(s) 858
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 46
BsaJI CCNNGG 4 cut(s) 115, 290, 839, 1227
BsaWI WCCGGW 2 cut(s) 190, 262
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 24, 160, 661, 800, 1374
Bse1I ACTGG 2 cut(s) 725, 1187
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 6 cut(s) 255, 396, 582, 711, 903, 1153
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 4 cut(s) 115, 290, 839, 1227
BseGI GGATG 4 cut(s) 670, 946, 991, 1240
BseLI CCNNNNNNNGG 6 cut(s) 23, 24, 160, 661, 800, 1374
BseMI GCAATG 6 cut(s) 255, 396, 582, 711, 903, 1153
BseMII CTCAG 4 cut(s) 173, 1073, 1320, 1368
BseNI ACTGG 2 cut(s) 725, 1187
BseSI GKGCMC 3 cut(s) 18, 362, 595
BseXI GCAGC 1 cut(s) 8
BseYI CCCAGC 3 cut(s) 542, 587, 734
Bsh1236I CGCG 1 cut(s) 242
BshFI GGCC 6 cut(s) 63, 89, 105, 120, 153, 395
BshNI GGYRCC 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 362
BsiHKCI CYCGRG 2 cut(s) 131, 840
BsiSI CCGG 6 cut(s) 78, 90, 150, 191, 263, 744
BslFI GGGAC 4 cut(s) 40, 97, 492, 1164
BslI CCNNNNNNNGG 6 cut(s) 23, 24, 160, 661, 800, 1374
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 4 cut(s) 40, 97, 492, 1164
BsmI GAATGC 2 cut(s) 697, 1158
BsnI GGCC 6 cut(s) 63, 89, 105, 120, 153, 395
BsoBI CYCGRG 2 cut(s) 131, 840
Bsp1286I GDGCHC 3 cut(s) 18, 362, 595
Bsp143I GATC 5 cut(s) 163, 658, 790, 1033, 1360
Bsp19I CCATGG 1 cut(s) 1227
BspACI CCGC 6 cut(s) 5, 24, 177, 296, 386, 552
BspANI GGCC 6 cut(s) 63, 89, 105, 120, 153, 395
BspCNI CTCAG 4 cut(s) 172, 1072, 1321, 1367
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 4 cut(s) 15, 396, 1180, 1435
BspPI GGATC 3 cut(s) 171, 785, 1355
BspQI GCTCTTC 2 cut(s) 264, 493
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 6 cut(s) 255, 396, 582, 711, 903, 1153
BsrI ACTGG 2 cut(s) 725, 1187
BssECI CCNNGG 4 cut(s) 115, 290, 839, 1227
BssMI GATC 5 cut(s) 163, 658, 790, 1033, 1360
BssT1I CCWWGG 2 cut(s) 290, 1227
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 4 cut(s) 379, 899, 992, 1178
Bst6I CTCTTC 4 cut(s) 264, 493, 1200, 1254
BstAPI GCANNNNNTGC 1 cut(s) 623
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 6 cut(s) 170, 174, 224, 1002, 1006, 1323
BstDEI CTNAG 4 cut(s) 159, 1059, 1329, 1354
BstDSI CCRYGG 1 cut(s) 1227
BstF5I GGATG 4 cut(s) 670, 946, 991, 1240
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 5 cut(s) 166, 661, 793, 1036, 1363
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 5 cut(s) 163, 658, 790, 1033, 1360
BstMWI GCNNNNNNNGC 6 cut(s) 69, 254, 392, 623, 701, 1064
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 2 cut(s) 859, 1325
BstPAI GACNNNNGTC 1 cut(s) 1086
BstSCI CCNGG 2 cut(s) 63, 742
BstSLI GKGCMC 3 cut(s) 18, 362, 595
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 858
Bsu36I CCTNAGG 1 cut(s) 159
BsuI GTATCC 1 cut(s) 450
BsuRI GGCC 6 cut(s) 63, 89, 105, 120, 153, 395
BtgI CCRYGG 1 cut(s) 1227
BtsCI GGATG 4 cut(s) 670, 946, 991, 1240
BtsI GCAGTG 2 cut(s) 360, 1397
BtsIMutI CAGTG 3 cut(s) 360, 1397, 1422
Cac8I GCNNGC 6 cut(s) 170, 174, 224, 1002, 1006, 1323
CaiI CAGNNNCTG 2 cut(s) 211, 1146
CciI TCATGA 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 136, 394, 1178
CseI GACGC 1 cut(s) 1401
CviAII CATG 8 cut(s) 109, 142, 530, 566, 628, 856, 1228, 1322
DdeI CTNAG 4 cut(s) 159, 1059, 1329, 1354
DpnI GATC 5 cut(s) 165, 660, 792, 1035, 1362
DpnII GATC 5 cut(s) 163, 658, 790, 1033, 1360
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 2 cut(s) 87, 151
Eam1104I CTCTTC 4 cut(s) 264, 493, 1200, 1254
EarI CTCTTC 4 cut(s) 264, 493, 1200, 1254
Eco130I CCWWGG 2 cut(s) 290, 1227
Eco147I AGGCCT 1 cut(s) 63
Eco32I GATATC 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 136, 1178
Eco57I CTGAAG 1 cut(s) 1238
Eco81I CCTNAGG 1 cut(s) 159
Eco88I CYCGRG 2 cut(s) 131, 840
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 63
EcoRV GATATC 1 cut(s) 309
EcoT14I CCWWGG 2 cut(s) 290, 1227
EcoT22I ATGCAT 3 cut(s) 649, 1323, 1327
ErhI CCWWGG 2 cut(s) 290, 1227
FaeI CATG 8 cut(s) 112, 145, 533, 569, 631, 859, 1231, 1325
FalI AAGNNNNNCTT 4 cut(s) 764, 796, 1236, 1268
FaqI GGGAC 4 cut(s) 40, 97, 492, 1164
FatI CATG 8 cut(s) 108, 141, 529, 565, 627, 855, 1227, 1321
FauI CCCGC 2 cut(s) 17, 545
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 5 cut(s) 6, 22, 177, 297, 387
FokI GGATG 4 cut(s) 677, 953, 998, 1247
Fsp4HI GCNGC 5 cut(s) 6, 22, 177, 297, 387
FspBI CTAG 1 cut(s) 761
GluI GCNGC 5 cut(s) 6, 22, 177, 297, 387
GsaI CCCAGC 3 cut(s) 546, 591, 738
HaeIII GGCC 6 cut(s) 63, 89, 105, 120, 153, 395
HapII CCGG 6 cut(s) 78, 90, 150, 191, 263, 744
HgaI GACGC 1 cut(s) 1401
Hin1II CATG 8 cut(s) 112, 145, 533, 569, 631, 859, 1231, 1325
HincII GTYRAC 2 cut(s) 127, 1091
HindII GTYRAC 2 cut(s) 127, 1091
HinfI GANTC 7 cut(s) 93, 202, 631, 775, 1010, 1346, 1451
HpaII CCGG 6 cut(s) 78, 90, 150, 191, 263, 744
HphI GGTGA 4 cut(s) 208, 977, 1091, 1378
Hpy166II GTNNAC 4 cut(s) 127, 360, 1091, 1151
Hpy188I TCNGA 7 cut(s) 236, 850, 888, 1062, 1330, 1345, 1426
Hpy8I GTNNAC 4 cut(s) 127, 360, 1091, 1151
Hpy99I CGWCG 2 cut(s) 34, 128
HpyAV CCTTC 3 cut(s) 4, 1350, 1374
HpyCH4III ACNGT 4 cut(s) 379, 899, 992, 1178
HpyCH4IV ACGT 2 cut(s) 32, 45
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 254, 392, 623, 701, 1064
HpyF3I CTNAG 4 cut(s) 159, 1059, 1329, 1354
HpySE526I ACGT 2 cut(s) 32, 45
Hsp92II CATG 8 cut(s) 112, 145, 533, 569, 631, 859, 1231, 1325
Kzo9I GATC 5 cut(s) 163, 658, 790, 1033, 1360
LguI GCTCTTC 2 cut(s) 264, 493
Lsp1109I GCAGC 1 cut(s) 8
LweI GCATC 5 cut(s) 613, 655, 931, 1028, 1334
MaeI CTAG 1 cut(s) 761
MaeII ACGT 2 cut(s) 32, 45
MaeIII GTNAC 7 cut(s) 213, 313, 805, 893, 899, 1079, 1384
MalI GATC 5 cut(s) 165, 660, 792, 1035, 1362
MboI GATC 5 cut(s) 163, 658, 790, 1033, 1360
MhlI GDGCHC 3 cut(s) 18, 362, 595
MluCI AATT 6 cut(s) 183, 599, 753, 834, 881, 1221
MluI ACGCGT 1 cut(s) 240
MlyI GAGTC 4 cut(s) 87, 625, 1340, 1445
Mph1103I ATGCAT 3 cut(s) 649, 1323, 1327
MroXI GAANNNNTTC 2 cut(s) 1248, 1251
MseI TTAA 7 cut(s) 230, 560, 771, 812, 981, 1128, 1308
MslI CAYNNNNRTG 2 cut(s) 326, 534
MspI CCGG 6 cut(s) 78, 90, 150, 191, 263, 744
MspR9I CCNGG 2 cut(s) 65, 744
Mva1269I GAATGC 2 cut(s) 697, 1158
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 6 cut(s) 69, 254, 392, 623, 701, 1064
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 1227
NdeII GATC 5 cut(s) 163, 658, 790, 1033, 1360
NlaIII CATG 8 cut(s) 112, 145, 533, 569, 631, 859, 1231, 1325
NlaIV GGNNCC 4 cut(s) 15, 396, 1180, 1435
NmeAIII GCCGAG 2 cut(s) 81, 96
NmuCI GTSAC 5 cut(s) 313, 893, 899, 1079, 1384
NsiI ATGCAT 3 cut(s) 649, 1323, 1327
NspI RCATGY 2 cut(s) 859, 1325
PaeI GCATGC 1 cut(s) 1325
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 63
PciSI GCTCTTC 2 cut(s) 264, 493
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 129
PctI GAATGC 2 cut(s) 697, 1158
PdmI GAANNNNTTC 2 cut(s) 1248, 1251
PfeI GAWTC 3 cut(s) 202, 775, 1010
PflMI CCANNNNNTGG 2 cut(s) 800, 1374
PkrI GCNGC 5 cut(s) 7, 23, 178, 298, 388
PleI GAGTC 4 cut(s) 87, 625, 1340, 1445
PpsI GAGTC 4 cut(s) 87, 625, 1340, 1445
Ppu21I YACGTR 1 cut(s) 46
PpuMI RGGWCCY 1 cut(s) 136
PshAI GACNNNNGTC 1 cut(s) 1086
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 63
PspFI CCCAGC 3 cut(s) 542, 587, 734
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 4 cut(s) 15, 396, 1180, 1435
PspPI GGNCC 3 cut(s) 136, 394, 1178
PspPPI RGGWCCY 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 131
PstNI CAGNNNCTG 2 cut(s) 211, 1146
RseI CAYNNNNRTG 2 cut(s) 326, 534
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 2 cut(s) 264, 493
SaqAI TTAA 7 cut(s) 230, 560, 771, 812, 981, 1128, 1308
SatI GCNGC 5 cut(s) 6, 22, 177, 297, 387
Sau3AI GATC 5 cut(s) 163, 658, 790, 1033, 1360
Sau96I GGNCC 3 cut(s) 136, 394, 1178
SchI GAGTC 4 cut(s) 87, 625, 1340, 1445
ScrFI CCNGG 2 cut(s) 65, 744
SduI GDGCHC 3 cut(s) 18, 362, 595
SfaNI GCATC 5 cut(s) 613, 655, 931, 1028, 1334
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 2 cut(s) 136, 1178
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 326, 534
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 1325
Sse9I AATT 6 cut(s) 183, 599, 753, 834, 881, 1221
SseBI AGGCCT 1 cut(s) 63
SsiI CCGC 6 cut(s) 5, 24, 177, 296, 386, 552
SspMI CTAG 1 cut(s) 761
StuI AGGCCT 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 63, 742
StyI CCWWGG 2 cut(s) 290, 1227
TaaI ACNGT 4 cut(s) 379, 899, 992, 1178
TaiI ACGT 2 cut(s) 35, 48
TaqI TCGA 3 cut(s) 126, 132, 1349
TasI AATT 6 cut(s) 183, 599, 753, 834, 881, 1221
TauI GCSGC 4 cut(s) 8, 179, 299, 389
TfiI GAWTC 3 cut(s) 202, 775, 1010
Tru1I TTAA 7 cut(s) 230, 560, 771, 812, 981, 1128, 1308
Tru9I TTAA 7 cut(s) 230, 560, 771, 812, 981, 1128, 1308
TscAI CASTG 3 cut(s) 360, 1404, 1422
TseFI GTSAC 5 cut(s) 313, 893, 899, 1079, 1384
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 5 cut(s) 313, 893, 899, 1079, 1384
TspDTI ATGAA 4 cut(s) 158, 518, 894, 1218
TspRI CASTG 3 cut(s) 360, 1404, 1422
Van91I CCANNNNNTGG 2 cut(s) 800, 1374
VneI GTGCAC 1 cut(s) 358
VpaK11BI GGWCC 2 cut(s) 136, 1178
XapI RAATTY 3 cut(s) 183, 834, 881
XceI RCATGY 2 cut(s) 859, 1325
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 2 cut(s) 1248, 1251
XspI CTAG 1 cut(s) 761
Zsp2I ATGCAT 3 cut(s) 649, 1323, 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.