Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0009069

Physical Location & Seq

Genomic Coordinates Forward (+)

1379142 .. 1383118

Gene Structure 3977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g02350.10

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGATGAATTTAAATTACTACGGTCTTGTAATATTCATCTTCACATGTAATATATTATTACTAGTTTATTATAAGGTTAAGTTCTCATCTTTCTTCTGCCGCGTTCTAATCGTCTCAATTAAACGCAAGTTATTCTTGTTAACGCGGGCACTTGCTCCACTGGCGCCGCTAAAAGCAAGTGTTTTTGTGGAAGCAAATAAAAAAATCGAGGGAACCGAACCGGCCAAAAAGAGATCCAGTACACCCAACCGAAAAGGTCCGAGAAACGCGCACGGCTTCAAATTTTGGTCTCCACTCTCCAGCAACCGAGCGACGGAGATGGCTGCGGTGGTGGCCTGCTGCGGGACGAAGTTCTTTTTGTACGTGCTGATAATCGTAGGCCTGGTATGCTTCGCCGGAATGATGGCCGGACTCACTCTCGGCCTCATGTCCCTCGGCCTCGTCGACCTCGAGGTCCTCATGAAGTCCGGCCGCCCTCAGGATCGCAAACACGCCGCTAAAATTTATCCGGTGGTGAAGAATCAGCACCTGTTACTTTGCACGCTTTTAATCGGAAACGCGTTAGCAATGGAGGCTCTTCCGGTGTTTTTGGATAGTCTTGTGCCTCCTTGGGCGGCCATTGTGATATCAGTCACTCTCATCCTGGTGTTTGGGGAGATATTGCCGCAAGCAGTCTGCACTCGCTATGGATTGACTGTTGGGGCGACAATGGCCCCATTTGTGCGCTTTCTTCTTGTTCTTTTCCTCCCCATTTCGTATCCCATTAGTAAGGTTCTGGATTATATGCTGGGTAAGGGACAATCTGCCCTTTTACGAAGAGCAGAGCTAAAGACTTTTGTGAATTTTCATGGAAATGAGGCTGGGAAAGGCGGGGATTTAACACATGATGAGACTACCATCATTGCTGGGGCACTTGAATTGACTGAAAAGACAGCAAAAGATGCTATGACTCCCATAACAAATGCATTTGCCCTTGATCTGGATGCACCTCTTACTTTGGACACATTGAATGCAATAATGGCAATGGGTCATAGTAGAGTTCCAGTTTATTGTGGGAAGCCCGGAAATATAATTGGTCTTGTTCTGGTTAAGAATCTCTTGATGGTTGATCCAGAAGGTGGAGTTACTTTGAGAAAACTGATGATCAGAAAAATTCCTCGGGTTTCAGAAGACATGCCTCTGTATGATATTCTAAATGAATTTCAGAAGGGTCACAGTCACATTGCTGTTGTCTTTAAGTATCCAAACGGGAAAAAGGATGCACTAAAGAGTGGCAAAGATGGCGAACCACCCGTGTTTAAGGATGATTGTAAGAAGCAAGCGGGCCAACCTGTGGCATTATTCAAGAAAGATGAAAAAGTGGGTTCAAGCAATGCTCTGAGTGCTTTGGACAATGGTGATGGAGGTCAACAATCTAAGAAAAGCCCATCAGCTACTCCCGCTTTTAAGAAACGACACAGAGGCTGTTCACATTGCATTCTGGACGTTGAGAACGGTCCCATTCCAGTTTTCCCAAACAATGAAGAGTTTGTAGGAGTAATTACCATGGAGGATGTCATTGAAGAACTTCTTCAGGAAGAGATATGGGATGAAACAGATGAGTACGTCGATATCCACAACAGGATTAAGGTCAATATGCATGCATCTCAGGAAAAGCCTTCTTACACGAACTCATCCAATCCTTCCGGACAATGGTCCCCTGTCAACCACGCCCCACTACTAACATCATCATCAGTGCCATCATATTCAGGTTCTTCTCAACATCAAGAGTCTGATAAGTTGACTGCTGTAGGTCCAATGCCAACGGACTCACATACCACAACTCATCAAGGTTCTGAAGGCGACACGTCAAGAGATCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS DUF21

Protein Analysis

621

Amino Acids

67.82

Weight (kDa)

8.66

Isoelectric Point (pI)

41.81

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47330 AT1G47330
fragaria_vesca	FvH4_2g39791
malus_domestica	MD08G1031700.v1.1 MD15G1027600.v1.1
prunus_persica	Prupe.1G379300_v2.0.a1
pyrus_communis	pycom08g02500 pycom15g02350
rosa_chinensis	RchiOBHm_Chr6g0301051
rosa_laevigata	RLG00000011281
rosa_multiflora	Rmu_co8014528.1_g000001 Rmu_sc0003168.1_g000011 Rmu_sc0023248.1_g000001 Rmu_sc0035551.1_g000002 Rmu_sc0039108.1_g000001
rosa_roxburghii	Rroxscaffold_7G00167220
rosa_rugosa	Rorug06G0300600
rosa_samantha	Rh6AG414200 Rh6AG414300 Rh6BG421200 Rh6CG428800 Rh6DG414200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	72
AasI	GACNNNNNNGTC	1 cut(s)	452
AbsI	CCTCGAGG	1 cut(s)	449
AccB1I	GGYRCC	1 cut(s)	163
AccB7I	CCANNNNNTGG	2 cut(s)	1118, 1333
AccI	GTMKAC	1 cut(s)	444
AccII	CGCG	4 cut(s)	102, 145, 269, 560
AccIII	TCCGGA	1 cut(s)	1685
AclWI	GGATC	3 cut(s)	228, 489, 1103
AcoI	YGGCCR	4 cut(s)	222, 405, 469, 615
AcsI	RAATTY	6 cut(s)	7, 281, 501, 841, 1152, 1199
AcuI	CTGAAG	2 cut(s)	1556, 1857
AcyI	GRCGYC	1 cut(s)	164
AfaI	GTAC	3 cut(s)	241, 362, 1604
AfiI	CCNNNNNNNGG	7 cut(s)	313, 342, 478, 979, 1118, 1333, 1692
AflIII	ACRYGT	3 cut(s)	44, 558, 1845
AgsI	TTSAA	6 cut(s)	280, 917, 1009, 1345, 1368, 1562
AhlI	ACTAGT	1 cut(s)	61
AjiI	CACGTC	1 cut(s)	1848
AjnI	CCWGG	2 cut(s)	381, 642
AluBI	AGCT	2 cut(s)	826, 1433
AluI	AGCT	2 cut(s)	826, 1433
Alw26I	GTCTC	3 cut(s)	118, 294, 884
AlwI	GGATC	3 cut(s)	228, 489, 1103
AlwNI	CAGNNNCTG	2 cut(s)	529, 1464
Ama87I	CYCGRG	2 cut(s)	449, 1158
Aor13HI	TCCGGA	1 cut(s)	1685
ApeKI	GCWGC	2 cut(s)	323, 339
ApoI	RAATTY	6 cut(s)	7, 281, 501, 841, 1152, 1199
Asp700I	GAANNNNTTC	2 cut(s)	1566, 1569
AspLEI	GCGC	3 cut(s)	166, 271, 726
AspS9I	GGNCC	7 cut(s)	257, 454, 712, 1324, 1496, 1695, 1793
AsuC2I	CCSGG	1 cut(s)	1062
AsuHPI	GGTGA	2 cut(s)	526, 1409
AvaI	CYCGRG	2 cut(s)	449, 1158
AvaII	GGWCC	5 cut(s)	257, 454, 1496, 1695, 1793
AxyI	CCTNAGG	1 cut(s)	477
BaeGI	GKGCMC	2 cut(s)	151, 913
BanI	GGYRCC	1 cut(s)	163
BbsI	GAAGAC	1 cut(s)	1176
BbvI	GCAGC	2 cut(s)	310, 326
BccI	CCATC	8 cut(s)	313, 397, 905, 1096, 1274, 1394, 1435, 1747
BceAI	ACGGC	1 cut(s)	289
BciT130I	CCWGG	2 cut(s)	383, 644
BciVI	GTATCC	2 cut(s)	768, 1251
BclI	TGATCA	1 cut(s)	1143
BcnI	CCSGG	1 cut(s)	1062
BcoDI	GTCTC	3 cut(s)	118, 294, 884
BcuI	ACTAGT	1 cut(s)	61
BfaI	CTAG	1 cut(s)	62
BfmI	CTRYAG	1 cut(s)	1788
BfoI	RGCGCY	1 cut(s)	167
BfuI	GTATCC	2 cut(s)	768, 1251
BisI	GCNGC	8 cut(s)	100, 167, 324, 340, 472, 495, 615, 665
BlsI	GCNGC	8 cut(s)	101, 168, 325, 341, 473, 496, 616, 666
Bme1390I	CCNGG	3 cut(s)	383, 644, 1062
Bme18I	GGWCC	5 cut(s)	257, 454, 1496, 1695, 1793
BmeT110I	CYCGRG	2 cut(s)	449, 1158
BmgBI	CACGTC	1 cut(s)	1848
BmgT120I	GGNCC	7 cut(s)	257, 454, 712, 1324, 1496, 1695, 1793
BmiI	GGNNCC	5 cut(s)	165, 214, 714, 1498, 1697
BmrFI	CCNGG	3 cut(s)	383, 644, 1062
BmsI	GCATC	4 cut(s)	931, 973, 1249, 1652
BoxI	GACNNNNGTC	1 cut(s)	1693
BpiI	GAAGAC	1 cut(s)	1176
BpmI	CTGGAG	1 cut(s)	283
BpuMI	CCSGG	1 cut(s)	1062
BsaAI	YACGTR	1 cut(s)	364
BsaHI	GRCGYC	1 cut(s)	164
BsaI	GGTCTC	1 cut(s)	294
BsaJI	CCNNGG	4 cut(s)	433, 608, 1157, 1545
BsaWI	WCCGGW	3 cut(s)	508, 580, 1685
Bsc4I	CCNNNNNNNGG	7 cut(s)	313, 342, 478, 979, 1118, 1333, 1692
Bse118I	RCCGGY	1 cut(s)	220
Bse1I	ACTGG	4 cut(s)	165, 237, 1043, 1505
Bse21I	CCTNAGG	1 cut(s)	477
Bse3DI	GCAATG	6 cut(s)	573, 900, 1029, 1221, 1378, 1471
BseAI	TCCGGA	1 cut(s)	1685
BseBI	CCWGG	2 cut(s)	383, 644
BseDI	CCNNGG	4 cut(s)	433, 608, 1157, 1545
BseGI	GGATG	7 cut(s)	639, 988, 1264, 1309, 1558, 1594, 1673
BseLI	CCNNNNNNNGG	7 cut(s)	313, 342, 478, 979, 1118, 1333, 1692
BseMI	GCAATG	6 cut(s)	573, 900, 1029, 1221, 1378, 1471
BseMII	CTCAG	3 cut(s)	491, 1370, 1661
BseNI	ACTGG	4 cut(s)	165, 237, 1043, 1505
BseSI	GKGCMC	2 cut(s)	151, 913
BseX3I	CGGCCG	1 cut(s)	469
BseXI	GCAGC	2 cut(s)	310, 326
BseYI	CCCAGC	3 cut(s)	787, 860, 905
BsgI	GTGCAG	1 cut(s)	661
Bsh1236I	CGCG	4 cut(s)	102, 145, 269, 560
Bsh1285I	CGRYCG	1 cut(s)	472
BshNI	GGYRCC	1 cut(s)	163
BsiEI	CGRYCG	1 cut(s)	472
BsiHKCI	CYCGRG	2 cut(s)	449, 1158
BsiSI	CCGG	8 cut(s)	221, 396, 408, 468, 509, 581, 1062, 1686
BslFI	GGGAC	5 cut(s)	358, 415, 810, 1482, 1681
BslI	CCNNNNNNNGG	7 cut(s)	313, 342, 478, 979, 1118, 1333, 1692
BsmAI	GTCTC	3 cut(s)	118, 294, 884
BsmBI	CGTCTC	1 cut(s)	118
BsmFI	GGGAC	5 cut(s)	358, 415, 810, 1482, 1681
BsmI	GAATGC	2 cut(s)	1015, 1476
Bso31I	GGTCTC	1 cut(s)	294
BsoBI	CYCGRG	2 cut(s)	449, 1158
Bsp1286I	GDGCHC	2 cut(s)	151, 913
Bsp13I	TCCGGA	1 cut(s)	1685
Bsp143I	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
Bsp19I	CCATGG	1 cut(s)	1545
BspCNI	CTCAG	3 cut(s)	490, 1371, 1660
BspEI	TCCGGA	1 cut(s)	1685
BspFNI	CGCG	4 cut(s)	102, 145, 269, 560
BspHI	TCATGA	1 cut(s)	459
BspLI	GGNNCC	5 cut(s)	165, 214, 714, 1498, 1697
BspPI	GGATC	3 cut(s)	228, 489, 1103
BspQI	GCTCTTC	2 cut(s)	582, 811
BspT107I	GGYRCC	1 cut(s)	163
BspTNI	GGTCTC	1 cut(s)	294
BsrDI	GCAATG	6 cut(s)	573, 900, 1029, 1221, 1378, 1471
BsrFI	RCCGGY	1 cut(s)	220
BsrI	ACTGG	4 cut(s)	165, 237, 1043, 1505
BssAI	RCCGGY	1 cut(s)	220
BssECI	CCNNGG	4 cut(s)	433, 608, 1157, 1545
BssMI	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
BssNI	GRCGYC	1 cut(s)	164
BssT1I	CCWWGG	2 cut(s)	608, 1545
Bst2UI	CCWGG	2 cut(s)	383, 644
Bst4CI	ACNGT	4 cut(s)	23, 697, 1217, 1496
Bst6I	CTCTTC	4 cut(s)	582, 811, 1518, 1572
BstACI	GRCGYC	1 cut(s)	164
BstAPI	GCANNNNNTGC	1 cut(s)	941
BstBAI	YACGTR	1 cut(s)	364
BstC8I	GCNNGC	7 cut(s)	147, 337, 542, 669, 1320, 1324, 1641
BstDEI	CTNAG	4 cut(s)	477, 1379, 1416, 1647
BstDSI	CCRYGG	1 cut(s)	1545
BstF5I	GGATG	7 cut(s)	639, 988, 1264, 1309, 1558, 1594, 1673
BstFNI	CGCG	4 cut(s)	102, 145, 269, 560
BstH2I	RGCGCY	1 cut(s)	167
BstHHI	GCGC	3 cut(s)	166, 271, 726
BstKTI	GATC	6 cut(s)	236, 484, 979, 1111, 1146, 1858
BstMAI	GTCTC	3 cut(s)	118, 294, 884
BstMBI	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
BstMCI	CGRYCG	1 cut(s)	472
BstNI	CCWGG	2 cut(s)	383, 644
BstNSI	RCATGY	3 cut(s)	48, 1177, 1643
BstPAI	GACNNNNGTC	1 cut(s)	1693
BstSCI	CCNGG	3 cut(s)	381, 642, 1060
BstSFI	CTRYAG	1 cut(s)	1788
BstSLI	GKGCMC	2 cut(s)	151, 913
BstUI	CGCG	4 cut(s)	102, 145, 269, 560
BstV1I	GCAGC	2 cut(s)	310, 326
BstV2I	GAAGAC	1 cut(s)	1176
BstX2I	RGATCY	1 cut(s)	233
BstYI	RGATCY	1 cut(s)	233
BstZI	CGGCCG	1 cut(s)	469
Bsu36I	CCTNAGG	1 cut(s)	477
BsuI	GTATCC	2 cut(s)	768, 1251
BtgI	CCRYGG	1 cut(s)	1545
BtrI	CACGTC	1 cut(s)	1848
BtsCI	GGATG	7 cut(s)	639, 988, 1264, 1309, 1558, 1594, 1673
BtsIMutI	CAGTG	2 cut(s)	158, 1740
Cac8I	GCNNGC	7 cut(s)	147, 337, 542, 669, 1320, 1324, 1641
CaiI	CAGNNNCTG	2 cut(s)	529, 1464
CciI	TCATGA	1 cut(s)	459
CfoI	GCGC	3 cut(s)	166, 271, 726
Cfr10I	RCCGGY	1 cut(s)	220
Cfr13I	GGNCC	7 cut(s)	257, 454, 712, 1324, 1496, 1695, 1793
Csp6I	GTAC	3 cut(s)	240, 361, 1603
CviAII	CATG	8 cut(s)	45, 427, 460, 848, 884, 1174, 1546, 1640
CviQI	GTAC	3 cut(s)	240, 361, 1603
DdeI	CTNAG	4 cut(s)	477, 1379, 1416, 1647
DinI	GGCGCC	1 cut(s)	165
DpnI	GATC	6 cut(s)	235, 483, 978, 1110, 1145, 1857
DpnII	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
DraI	TTTAAA	1 cut(s)	12
DrdI	GACNNNNNNGTC	1 cut(s)	452
DseDI	GACNNNNNNGTC	1 cut(s)	452
EaeI	YGGCCR	4 cut(s)	222, 405, 469, 615
EagI	CGGCCG	1 cut(s)	469
Eam1104I	CTCTTC	4 cut(s)	582, 811, 1518, 1572
EarI	CTCTTC	4 cut(s)	582, 811, 1518, 1572
EclXI	CGGCCG	1 cut(s)	469
Eco130I	CCWWGG	2 cut(s)	608, 1545
Eco147I	AGGCCT	1 cut(s)	381
Eco31I	GGTCTC	1 cut(s)	294
Eco32I	GATATC	2 cut(s)	627, 1612
Eco47I	GGWCC	5 cut(s)	257, 454, 1496, 1695, 1793
Eco52I	CGGCCG	1 cut(s)	469
Eco57I	CTGAAG	2 cut(s)	1556, 1857
Eco81I	CCTNAGG	1 cut(s)	477
Eco88I	CYCGRG	2 cut(s)	449, 1158
EcoO109I	RGGNCCY	1 cut(s)	454
EcoRII	CCWGG	2 cut(s)	381, 642
EcoRV	GATATC	2 cut(s)	627, 1612
EcoT14I	CCWWGG	2 cut(s)	608, 1545
EcoT22I	ATGCAT	3 cut(s)	967, 1641, 1645
EgeI	GGCGCC	1 cut(s)	165
EheI	GGCGCC	1 cut(s)	165
ErhI	CCWWGG	2 cut(s)	608, 1545
Esp3I	CGTCTC	1 cut(s)	118
FaeI	CATG	8 cut(s)	48, 430, 463, 851, 887, 1177, 1549, 1643
FalI	AAGNNNNNCTT	6 cut(s)	119, 151, 1082, 1114, 1554, 1586
FaqI	GGGAC	5 cut(s)	358, 415, 810, 1482, 1681
FatI	CATG	8 cut(s)	44, 426, 459, 847, 883, 1173, 1545, 1639
FauI	CCCGC	5 cut(s)	138, 335, 863, 1315, 1447
FbaI	TGATCA	1 cut(s)	1143
FblI	GTMKAC	1 cut(s)	444
Fnu4HI	GCNGC	8 cut(s)	100, 167, 324, 340, 472, 495, 615, 665
FokI	GGATG	7 cut(s)	626, 995, 1271, 1316, 1565, 1601, 1660
Fsp4HI	GCNGC	8 cut(s)	100, 167, 324, 340, 472, 495, 615, 665
FspBI	CTAG	1 cut(s)	62
GlaI	GCGC	3 cut(s)	165, 270, 725
GluI	GCNGC	8 cut(s)	100, 167, 324, 340, 472, 495, 615, 665
GsaI	CCCAGC	3 cut(s)	791, 864, 909
GsuI	CTGGAG	1 cut(s)	283
HaeII	RGCGCY	1 cut(s)	167
HapII	CCGG	8 cut(s)	221, 396, 408, 468, 509, 581, 1062, 1686
HhaI	GCGC	3 cut(s)	166, 271, 726
Hin1I	GRCGYC	1 cut(s)	164
Hin1II	CATG	8 cut(s)	48, 430, 463, 851, 887, 1177, 1549, 1643
Hin6I	GCGC	3 cut(s)	164, 269, 724
HinP1I	GCGC	3 cut(s)	164, 269, 724
HincII	GTYRAC	5 cut(s)	141, 445, 1409, 1705, 1782
HindII	GTYRAC	5 cut(s)	141, 445, 1409, 1705, 1782
HinfI	GANTC	6 cut(s)	411, 520, 949, 1093, 1769, 1808
HpaI	GTTAAC	1 cut(s)	141
HpaII	CCGG	8 cut(s)	221, 396, 408, 468, 509, 581, 1062, 1686
HphI	GGTGA	2 cut(s)	526, 1409
Hpy166II	GTNNAC	7 cut(s)	141, 242, 445, 1409, 1469, 1705, 1782
Hpy188I	TCNGA	8 cut(s)	261, 554, 1148, 1168, 1206, 1380, 1774, 1837
Hpy8I	GTNNAC	7 cut(s)	141, 242, 445, 1409, 1469, 1705, 1782
Hpy99I	CGWCG	3 cut(s)	316, 446, 1610
HpyAV	CCTTC	5 cut(s)	1109, 1201, 1668, 1692, 1832
HpyCH4III	ACNGT	4 cut(s)	23, 697, 1217, 1496
HpyCH4IV	ACGT	4 cut(s)	363, 1485, 1605, 1847
HpyCH4V	TGCA	9 cut(s)	540, 678, 965, 986, 1013, 1262, 1476, 1639, 1643
HpyF3I	CTNAG	4 cut(s)	477, 1379, 1416, 1647
HpySE526I	ACGT	4 cut(s)	363, 1485, 1605, 1847
Hsp92I	GRCGYC	1 cut(s)	164
Hsp92II	CATG	8 cut(s)	48, 430, 463, 851, 887, 1177, 1549, 1643
HspAI	GCGC	3 cut(s)	164, 269, 724
KasI	GGCGCC	1 cut(s)	163
Kpn2I	TCCGGA	1 cut(s)	1685
Ksp22I	TGATCA	1 cut(s)	1143
KspAI	GTTAAC	1 cut(s)	141
Kzo9I	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
LguI	GCTCTTC	2 cut(s)	582, 811
LmnI	GCTCC	1 cut(s)	160
Lsp1109I	GCAGC	2 cut(s)	310, 326
LweI	GCATC	4 cut(s)	931, 973, 1249, 1652
MaeI	CTAG	1 cut(s)	62
MaeII	ACGT	4 cut(s)	363, 1485, 1605, 1847
MaeIII	GTNAC	5 cut(s)	531, 631, 1123, 1211, 1217
MalI	GATC	6 cut(s)	235, 483, 978, 1110, 1145, 1857
MboI	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
MflI	RGATCY	1 cut(s)	233
MhlI	GDGCHC	2 cut(s)	151, 913
MluI	ACGCGT	1 cut(s)	558
Mly113I	GGCGCC	1 cut(s)	164
MlyI	GAGTC	4 cut(s)	405, 943, 1778, 1802
Mph1103I	ATGCAT	3 cut(s)	967, 1641, 1645
MroI	TCCGGA	1 cut(s)	1685
MroXI	GAANNNNTTC	2 cut(s)	1566, 1569
MslI	CAYNNNNRTG	2 cut(s)	644, 852
MspI	CCGG	8 cut(s)	221, 396, 408, 468, 509, 581, 1062, 1686
MspR9I	CCNGG	3 cut(s)	383, 644, 1062
Mva1269I	GAATGC	2 cut(s)	1015, 1476
MvaI	CCWGG	2 cut(s)	383, 644
MvnI	CGCG	4 cut(s)	102, 145, 269, 560
NarI	GGCGCC	1 cut(s)	164
NciI	CCSGG	1 cut(s)	1062
NcoI	CCATGG	1 cut(s)	1545
NdeII	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
NlaIII	CATG	8 cut(s)	48, 430, 463, 851, 887, 1177, 1549, 1643
NlaIV	GGNNCC	5 cut(s)	165, 214, 714, 1498, 1697
NmeAIII	GCCGAG	2 cut(s)	399, 414
NmuCI	GTSAC	3 cut(s)	631, 1211, 1217
NsiI	ATGCAT	3 cut(s)	967, 1641, 1645
NspI	RCATGY	3 cut(s)	48, 1177, 1643
PaeI	GCATGC	1 cut(s)	1643
PaeR7I	CTCGAG	1 cut(s)	449
PagI	TCATGA	1 cut(s)	459
PceI	AGGCCT	1 cut(s)	381
PciI	ACATGT	1 cut(s)	44
PciSI	GCTCTTC	2 cut(s)	582, 811
PcsI	WCGNNNNNNNCGW	3 cut(s)	213, 441, 447
PctI	GAATGC	2 cut(s)	1015, 1476
PdmI	GAANNNNTTC	2 cut(s)	1566, 1569
PfeI	GAWTC	2 cut(s)	520, 1093
PflMI	CCANNNNNTGG	2 cut(s)	1118, 1333
PkrI	GCNGC	8 cut(s)	101, 168, 325, 341, 473, 496, 616, 666
PleI	GAGTC	4 cut(s)	405, 943, 1777, 1802
PluTI	GGCGCC	1 cut(s)	167
PpsI	GAGTC	4 cut(s)	405, 943, 1777, 1802
Ppu21I	YACGTR	1 cut(s)	364
PpuMI	RGGWCCY	1 cut(s)	454
PscI	ACATGT	1 cut(s)	44
PshAI	GACNNNNGTC	1 cut(s)	1693
PsiI	TTATAA	1 cut(s)	72
Psp5II	RGGWCCY	1 cut(s)	454
Psp6I	CCWGG	2 cut(s)	381, 642
PspFI	CCCAGC	3 cut(s)	787, 860, 905
PspGI	CCWGG	2 cut(s)	381, 642
PspN4I	GGNNCC	5 cut(s)	165, 214, 714, 1498, 1697
PspPI	GGNCC	7 cut(s)	257, 454, 712, 1324, 1496, 1695, 1793
PspPPI	RGGWCCY	1 cut(s)	454
PspXI	VCTCGAGB	1 cut(s)	449
PstNI	CAGNNNCTG	2 cut(s)	529, 1464
PsuI	RGATCY	1 cut(s)	233
RsaI	GTAC	3 cut(s)	241, 362, 1604
RsaNI	GTAC	3 cut(s)	240, 361, 1603
RseI	CAYNNNNRTG	2 cut(s)	644, 852
SalI	GTCGAC	1 cut(s)	443
SapI	GCTCTTC	2 cut(s)	582, 811
SatI	GCNGC	8 cut(s)	100, 167, 324, 340, 472, 495, 615, 665
Sau3AI	GATC	6 cut(s)	233, 481, 976, 1108, 1143, 1855
Sau96I	GGNCC	7 cut(s)	257, 454, 712, 1324, 1496, 1695, 1793
SchI	GAGTC	4 cut(s)	405, 943, 1778, 1802
ScrFI	CCNGG	3 cut(s)	383, 644, 1062
SduI	GDGCHC	2 cut(s)	151, 913
SfaNI	GCATC	4 cut(s)	931, 973, 1249, 1652
SfcI	CTRYAG	1 cut(s)	1788
SfoI	GGCGCC	1 cut(s)	165
Sfr274I	CTCGAG	1 cut(s)	449
SinI	GGWCC	5 cut(s)	257, 454, 1496, 1695, 1793
SlaI	CTCGAG	1 cut(s)	449
SmiI	ATTTAAAT	1 cut(s)	12
SmiMI	CAYNNNNRTG	2 cut(s)	644, 852
SmlI	CTYRAG	1 cut(s)	449
SmoI	CTYRAG	1 cut(s)	449
SpeI	ACTAGT	1 cut(s)	61
SphI	GCATGC	1 cut(s)	1643
SseBI	AGGCCT	1 cut(s)	381
SspDI	GGCGCC	1 cut(s)	163
SspI	AATATT	1 cut(s)	33
SspMI	CTAG	1 cut(s)	62
StuI	AGGCCT	1 cut(s)	381
StyD4I	CCNGG	3 cut(s)	381, 642, 1060
StyI	CCWWGG	2 cut(s)	608, 1545
SwaI	ATTTAAAT	1 cut(s)	12
TaaI	ACNGT	4 cut(s)	23, 697, 1217, 1496
TaiI	ACGT	4 cut(s)	366, 1488, 1608, 1850
TaqI	TCGA	4 cut(s)	207, 444, 450, 1608
TatI	WGTACW	1 cut(s)	239
TauI	GCSGC	6 cut(s)	102, 169, 474, 497, 617, 667
TfiI	GAWTC	2 cut(s)	520, 1093
TscAI	CASTG	2 cut(s)	165, 1740
TseFI	GTSAC	3 cut(s)	631, 1211, 1217
TseI	GCWGC	2 cut(s)	323, 339
Tsp45I	GTSAC	3 cut(s)	631, 1211, 1217
TspDTI	ATGAA	8 cut(s)	20, 25, 476, 836, 1212, 1368, 1536, 1605
TspGWI	ACGGA	2 cut(s)	329, 1820
TspRI	CASTG	2 cut(s)	165, 1740
Van91I	CCANNNNNTGG	2 cut(s)	1118, 1333
VpaK11BI	GGWCC	5 cut(s)	257, 454, 1496, 1695, 1793
XapI	RAATTY	6 cut(s)	7, 281, 501, 841, 1152, 1199
XceI	RCATGY	3 cut(s)	48, 1177, 1643
XhoI	CTCGAG	1 cut(s)	449
XmiI	GTMKAC	1 cut(s)	444
XmnI	GAANNNNTTC	2 cut(s)	1566, 1569
XspI	CTAG	1 cut(s)	62
Zsp2I	ATGCAT	3 cut(s)	967, 1641, 1645

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.