MD15G1027600.v1.1

methyltransferase PMT28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009069
Physical Location & Seq
Forward (+)
1624594 .. 1628727
4134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1027600.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGCTGCGGTGGTGGCCTGCTGCGGGACGAAGTTCTTTCTGTACTTGCTGATAATCGTAGGCCTGGTATGCTTCGCCGGAATGATGGCCGGACTCACTCTCGGCCTCATGTCCCTCGGCCTCGTCGACCTCGAGGTCCTCATGAAGTCCGGCCGCCCTCAGGATCGCAAACACGCCGCTAAAATTTATCCGGTGGTGAAGAATCAGCACCTGTTACTTTGCACTCTTTTAATCGGAAACGCGTTAGCAATGGAGGCTCTTCCGGTGTTTTTGGATAGTCTTGTGCCTCCTTGGGCGGCCATTGTGTTATCGGTCACTCTCATCCTGGTGTTTGGGGAGATATTGCCGCAAGCAGTCTGCACTCGCTATGGATTGACTGTTGGGGCGACAATGGCCCCATTTGTGCGTTTTCTTCTTGTTCTTTTCCTCCCCATTTCGTATCCCATTAGCAAGGTTCTGGATTATATGCTGGGTAAGGGACAATCTGCTCTTTTACGAAGAGCAGAGCTAAAGACTTTTGTGAATTTTCATGGAAATGAGGCTGGGAAAGGTGGGGATTTAACACATGATGAGACTACCATCATTGCTGGGGCACTTGAATTGACTGAAAAGACAGCAAAAGATGCTATGACTCCCATAACAAATGCATTTGCCCTTGATCTGGATGCACCTGTTACTTTGGACACATTGAATGCAATAATGGTAATGGGTCATAGTAGAGTTCCAGTTTATTGTGGGAAGCCCGGAAATATAATTGGTCTTGTTCTGGTTAAGAATCTCTTGATGGTTGATCCAGAAGGTGGAGTTACTTTGAGAAAACTGATGATCAGAAAAATTCCTCGGGTTTCAGAAGACATGCCTCTGTATGATATTCTAAATGAATTTCAGAAAGGTCACAGTCACATTGCTGTTGTCTTTAAGTATCCAAACGGGAAAAAGGATGCACTAAAGAATGGCAAAGATGGCGAACCACCTGTGTTTAAGGATGATTGTAAGAAGCAAGCGGGCCAACCTGTGGCATCATTCAAGAAAGATGAAAAAGTGGGTTCAAGCAATGCTCTGAGTGCTTTGGACAATGGTGATGGAGGTCAACAATCTAAGAAAAGCCCATCAGCTACTCCTGCATTTAAGAAACGACACAGAGGCTGTTCACATTGCATTCTGGACGTTGAGAACGGTCCCATTCCAGTTTTCCCAGACAATGAAGAGTTTGTAGGAGTAATTACCATGGAGGATGTCATCGAAGAACTTCTTCAGGAAGAGATATGGGATGAAACAGATGAGTATGTCGATATCCACAACAGGATTAAGGTCAACATGCATGCATCTCAGGAAAAGCCTTCTTACTCGAACCCACCCAATCCTTCCGGACAACGGTCACCACCTGTCAACCGCGCCCCACTGTTAACATCATCATCAGTGCCATTATATTCAGGTTCTACTCAACATCAAGACTCTGATAAGGTGACTGCTGTAGATCCAATGCCAACGGACTCACATACCACAACTCATCAAGGTTCTGAAGGCGACACGTCAAGAGATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF21 Methyltransf_29

Protein Analysis

516

Amino Acids

55.92

Weight (kDa)

6.12

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 17 - 187 8.8e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AbsI CCTCGAGG 1 cut(s) 131
AccB7I CCANNNNNTGG 2 cut(s) 800, 1015
AccI GTMKAC 1 cut(s) 126
AccII CGCG 2 cut(s) 242, 1395
AccIII TCCGGA 1 cut(s) 1367
AciI CCGC 8 cut(s) 8, 24, 154, 177, 296, 347, 1004, 1393
AclWI GGATC 3 cut(s) 171, 785, 1472
AcoI YGGCCR 3 cut(s) 87, 151, 297
AcsI RAATTY 4 cut(s) 183, 523, 834, 881
AcuI CTGAAG 2 cut(s) 1238, 1542
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 6 cut(s) 24, 160, 661, 800, 1015, 1374
AflIII ACRYGT 2 cut(s) 240, 1530
AgsI TTSAA 4 cut(s) 599, 691, 1027, 1050
AjiI CACGTC 1 cut(s) 1533
AjnI CCWGG 2 cut(s) 63, 324
AluBI AGCT 2 cut(s) 508, 1115
AluI AGCT 2 cut(s) 508, 1115
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 3 cut(s) 171, 785, 1472
AlwNI CAGNNNCTG 2 cut(s) 211, 1146
Ama87I CYCGRG 2 cut(s) 131, 840
Aor13HI TCCGGA 1 cut(s) 1367
AoxI GGCC 9 cut(s) 15, 61, 87, 103, 118, 151, 297, 393, 1006
ApeKI GCWGC 2 cut(s) 5, 21
ApoI RAATTY 4 cut(s) 183, 523, 834, 881
Asp700I GAANNNNTTC 2 cut(s) 1248, 1251
AspLEI GCGC 1 cut(s) 1397
AspS9I GGNCC 4 cut(s) 136, 394, 1006, 1178
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 4 cut(s) 208, 1091, 1371, 1477
AvaI CYCGRG 2 cut(s) 131, 840
AvaII GGWCC 2 cut(s) 136, 1178
AxyI CCTNAGG 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 858
BbvI GCAGC 1 cut(s) 8
BccI CCATC 6 cut(s) 79, 587, 778, 956, 1076, 1117
BciT130I CCWGG 2 cut(s) 65, 326
BciVI GTATCC 2 cut(s) 450, 933
BclI TGATCA 1 cut(s) 825
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 1473
BfuI GTATCC 2 cut(s) 450, 933
BisI GCNGC 6 cut(s) 6, 22, 154, 177, 297, 347
BlsI GCNGC 6 cut(s) 7, 23, 155, 178, 298, 348
Bme1390I CCNGG 3 cut(s) 65, 326, 744
Bme18I GGWCC 2 cut(s) 136, 1178
BmeT110I CYCGRG 2 cut(s) 131, 840
BmgBI CACGTC 1 cut(s) 1533
BmgT120I GGNCC 4 cut(s) 136, 394, 1006, 1178
BmiI GGNNCC 2 cut(s) 396, 1180
BmrFI CCNGG 3 cut(s) 65, 326, 744
BmsI GCATC 5 cut(s) 613, 655, 931, 1028, 1334
BoxI GACNNNNGTC 1 cut(s) 1375
BpiI GAAGAC 1 cut(s) 858
BpuMI CCSGG 1 cut(s) 744
BsaJI CCNNGG 4 cut(s) 115, 290, 839, 1227
BsaWI WCCGGW 3 cut(s) 190, 262, 1367
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 160, 661, 800, 1015, 1374
Bse1I ACTGG 2 cut(s) 725, 1187
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 5 cut(s) 255, 582, 903, 1060, 1153
BseAI TCCGGA 1 cut(s) 1367
BseBI CCWGG 2 cut(s) 65, 326
BseDI CCNNGG 4 cut(s) 115, 290, 839, 1227
BseGI GGATG 6 cut(s) 321, 670, 946, 991, 1240, 1276
BseLI CCNNNNNNNGG 6 cut(s) 24, 160, 661, 800, 1015, 1374
BseMI GCAATG 5 cut(s) 255, 582, 903, 1060, 1153
BseMII CTCAG 3 cut(s) 173, 1052, 1343
BseNI ACTGG 2 cut(s) 725, 1187
BseSI GKGCMC 1 cut(s) 595
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 8
BseYI CCCAGC 3 cut(s) 469, 542, 587
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 242, 1395
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 9 cut(s) 17, 63, 89, 105, 120, 153, 299, 395, 1008
BsiEI CGRYCG 1 cut(s) 154
BsiHKCI CYCGRG 2 cut(s) 131, 840
BsiSI CCGG 7 cut(s) 78, 90, 150, 191, 263, 744, 1368
BslFI GGGAC 4 cut(s) 40, 97, 492, 1164
BslI CCNNNNNNNGG 6 cut(s) 24, 160, 661, 800, 1015, 1374
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 4 cut(s) 40, 97, 492, 1164
BsmI GAATGC 2 cut(s) 697, 1158
BsnI GGCC 9 cut(s) 17, 63, 89, 105, 120, 153, 299, 395, 1008
BsoBI CYCGRG 2 cut(s) 131, 840
Bsp1286I GDGCHC 1 cut(s) 595
Bsp13I TCCGGA 1 cut(s) 1367
Bsp143I GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
Bsp19I CCATGG 1 cut(s) 1227
BspACI CCGC 8 cut(s) 8, 24, 154, 177, 296, 347, 1004, 1393
BspANI GGCC 9 cut(s) 17, 63, 89, 105, 120, 153, 299, 395, 1008
BspCNI CTCAG 3 cut(s) 172, 1053, 1342
BspEI TCCGGA 1 cut(s) 1367
BspFNI CGCG 2 cut(s) 242, 1395
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 396, 1180
BspPI GGATC 3 cut(s) 171, 785, 1472
BspQI GCTCTTC 2 cut(s) 264, 493
BsrDI GCAATG 5 cut(s) 255, 582, 903, 1060, 1153
BsrI ACTGG 2 cut(s) 725, 1187
BssECI CCNNGG 4 cut(s) 115, 290, 839, 1227
BssMI GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
BssT1I CCWWGG 2 cut(s) 290, 1227
Bst2UI CCWGG 2 cut(s) 65, 326
Bst4CI ACNGT 5 cut(s) 379, 899, 1178, 1377, 1404
Bst6I CTCTTC 4 cut(s) 264, 493, 1200, 1254
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 5 cut(s) 19, 351, 1002, 1006, 1323
BstDEI CTNAG 4 cut(s) 159, 1061, 1098, 1329
BstDSI CCRYGG 1 cut(s) 1227
BstEII GGTNACC 1 cut(s) 1377
BstF5I GGATG 6 cut(s) 321, 670, 946, 991, 1240, 1276
BstFNI CGCG 2 cut(s) 242, 1395
BstHHI GCGC 1 cut(s) 1397
BstKTI GATC 6 cut(s) 166, 661, 793, 828, 1480, 1543
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 8 cut(s) 14, 69, 254, 392, 623, 963, 1064, 1121
BstNI CCWGG 2 cut(s) 65, 326
BstNSI RCATGY 3 cut(s) 859, 1321, 1325
BstPAI GACNNNNGTC 1 cut(s) 1375
BstPI GGTNACC 1 cut(s) 1377
BstSCI CCNGG 3 cut(s) 63, 324, 742
BstSFI CTRYAG 1 cut(s) 1473
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 2 cut(s) 242, 1395
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 858
BstX2I RGATCY 1 cut(s) 1477
BstYI RGATCY 1 cut(s) 1477
BstZI CGGCCG 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 159
BsuI GTATCC 2 cut(s) 450, 933
BsuRI GGCC 9 cut(s) 17, 63, 89, 105, 120, 153, 299, 395, 1008
BtgI CCRYGG 1 cut(s) 1227
BtrI CACGTC 1 cut(s) 1533
BtsCI GGATG 6 cut(s) 321, 670, 946, 991, 1240, 1276
BtsIMutI CAGTG 3 cut(s) 1400, 1425, 1543
Cac8I GCNNGC 5 cut(s) 19, 351, 1002, 1006, 1323
CaiI CAGNNNCTG 2 cut(s) 211, 1146
CciI TCATGA 1 cut(s) 141
CfoI GCGC 1 cut(s) 1397
Cfr13I GGNCC 4 cut(s) 136, 394, 1006, 1178
Csp6I GTAC 1 cut(s) 43
CviAII CATG 8 cut(s) 109, 142, 530, 566, 856, 1228, 1318, 1322
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 4 cut(s) 159, 1061, 1098, 1329
DpnI GATC 6 cut(s) 165, 660, 792, 827, 1479, 1542
DpnII GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 3 cut(s) 87, 151, 297
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 4 cut(s) 264, 493, 1200, 1254
EarI CTCTTC 4 cut(s) 264, 493, 1200, 1254
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 290, 1227
Eco147I AGGCCT 1 cut(s) 63
Eco32I GATATC 1 cut(s) 1294
Eco47I GGWCC 2 cut(s) 136, 1178
Eco52I CGGCCG 1 cut(s) 151
Eco57I CTGAAG 2 cut(s) 1238, 1542
Eco81I CCTNAGG 1 cut(s) 159
Eco88I CYCGRG 2 cut(s) 131, 840
Eco91I GGTNACC 1 cut(s) 1377
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 1377
EcoRII CCWGG 2 cut(s) 63, 324
EcoRV GATATC 1 cut(s) 1294
EcoT14I CCWWGG 2 cut(s) 290, 1227
EcoT22I ATGCAT 3 cut(s) 649, 1323, 1327
ErhI CCWWGG 2 cut(s) 290, 1227
FaeI CATG 8 cut(s) 112, 145, 533, 569, 859, 1231, 1321, 1325
FalI AAGNNNNNCTT 4 cut(s) 764, 796, 1236, 1268
FaqI GGGAC 4 cut(s) 40, 97, 492, 1164
FatI CATG 8 cut(s) 108, 141, 529, 565, 855, 1227, 1317, 1321
FauI CCCGC 2 cut(s) 17, 997
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 6 cut(s) 6, 22, 154, 177, 297, 347
FokI GGATG 6 cut(s) 308, 677, 953, 998, 1247, 1283
Fsp4HI GCNGC 6 cut(s) 6, 22, 154, 177, 297, 347
GlaI GCGC 1 cut(s) 1396
GluI GCNGC 6 cut(s) 6, 22, 154, 177, 297, 347
GsaI CCCAGC 3 cut(s) 473, 546, 591
HaeIII GGCC 9 cut(s) 17, 63, 89, 105, 120, 153, 299, 395, 1008
HapII CCGG 7 cut(s) 78, 90, 150, 191, 263, 744, 1368
HhaI GCGC 1 cut(s) 1397
Hin1II CATG 8 cut(s) 112, 145, 533, 569, 859, 1231, 1321, 1325
Hin6I GCGC 1 cut(s) 1395
HinP1I GCGC 1 cut(s) 1395
HincII GTYRAC 5 cut(s) 127, 1091, 1315, 1390, 1407
HindII GTYRAC 5 cut(s) 127, 1091, 1315, 1390, 1407
HinfI GANTC 6 cut(s) 93, 202, 631, 775, 1454, 1493
HpaI GTTAAC 1 cut(s) 1407
HpaII CCGG 7 cut(s) 78, 90, 150, 191, 263, 744, 1368
HphI GGTGA 4 cut(s) 208, 1091, 1371, 1477
Hpy166II GTNNAC 6 cut(s) 127, 1091, 1151, 1315, 1390, 1407
Hpy188I TCNGA 7 cut(s) 236, 830, 850, 888, 1062, 1459, 1522
Hpy8I GTNNAC 6 cut(s) 127, 1091, 1151, 1315, 1390, 1407
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 4 cut(s) 791, 1350, 1374, 1517
HpyCH4III ACNGT 5 cut(s) 379, 899, 1178, 1377, 1404
HpyCH4IV ACGT 2 cut(s) 1167, 1532
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 69, 254, 392, 623, 963, 1064, 1121
HpyF3I CTNAG 4 cut(s) 159, 1061, 1098, 1329
HpySE526I ACGT 2 cut(s) 1167, 1532
Hsp92II CATG 8 cut(s) 112, 145, 533, 569, 859, 1231, 1321, 1325
HspAI GCGC 1 cut(s) 1395
Kpn2I TCCGGA 1 cut(s) 1367
Ksp22I TGATCA 1 cut(s) 825
KspAI GTTAAC 1 cut(s) 1407
Kzo9I GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
LguI GCTCTTC 2 cut(s) 264, 493
Lsp1109I GCAGC 1 cut(s) 8
LweI GCATC 5 cut(s) 613, 655, 931, 1028, 1334
MaeII ACGT 2 cut(s) 1167, 1532
MaeIII GTNAC 8 cut(s) 213, 313, 673, 805, 893, 899, 1377, 1465
MalI GATC 6 cut(s) 165, 660, 792, 827, 1479, 1542
MboI GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
MboII GAAGA 9 cut(s) 211, 251, 404, 510, 863, 1217, 1244, 1256, 1271
MflI RGATCY 1 cut(s) 1477
MhlI GDGCHC 1 cut(s) 595
MluCI AATT 7 cut(s) 183, 523, 599, 753, 834, 881, 1221
MluI ACGCGT 1 cut(s) 240
MlyI GAGTC 4 cut(s) 87, 625, 1448, 1487
Mph1103I ATGCAT 3 cut(s) 649, 1323, 1327
MroI TCCGGA 1 cut(s) 1367
MroXI GAANNNNTTC 2 cut(s) 1248, 1251
MseI TTAA 8 cut(s) 230, 560, 771, 918, 981, 1128, 1308, 1406
MslI CAYNNNNRTG 2 cut(s) 326, 534
MspI CCGG 7 cut(s) 78, 90, 150, 191, 263, 744, 1368
MspR9I CCNGG 3 cut(s) 65, 326, 744
Mva1269I GAATGC 2 cut(s) 697, 1158
MvaI CCWGG 2 cut(s) 65, 326
MvnI CGCG 2 cut(s) 242, 1395
MwoI GCNNNNNNNGC 8 cut(s) 14, 69, 254, 392, 623, 963, 1064, 1121
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 1227
NdeII GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
NlaIII CATG 8 cut(s) 112, 145, 533, 569, 859, 1231, 1321, 1325
NlaIV GGNNCC 2 cut(s) 396, 1180
NmeAIII GCCGAG 2 cut(s) 81, 96
NmuCI GTSAC 5 cut(s) 313, 893, 899, 1377, 1465
NsiI ATGCAT 3 cut(s) 649, 1323, 1327
NspI RCATGY 3 cut(s) 859, 1321, 1325
PaeI GCATGC 1 cut(s) 1325
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 63
PciSI GCTCTTC 2 cut(s) 264, 493
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 129
PctI GAATGC 2 cut(s) 697, 1158
PdmI GAANNNNTTC 2 cut(s) 1248, 1251
PfeI GAWTC 2 cut(s) 202, 775
PflMI CCANNNNNTGG 2 cut(s) 800, 1015
PkrI GCNGC 6 cut(s) 7, 23, 155, 178, 298, 348
PleI GAGTC 4 cut(s) 87, 625, 1448, 1487
PpsI GAGTC 4 cut(s) 87, 625, 1448, 1487
PpuMI RGGWCCY 1 cut(s) 136
PshAI GACNNNNGTC 1 cut(s) 1375
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 63, 324
PspEI GGTNACC 1 cut(s) 1377
PspFI CCCAGC 3 cut(s) 469, 542, 587
PspGI CCWGG 2 cut(s) 63, 324
PspN4I GGNNCC 2 cut(s) 396, 1180
PspPI GGNCC 4 cut(s) 136, 394, 1006, 1178
PspPPI RGGWCCY 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 131
PstNI CAGNNNCTG 2 cut(s) 211, 1146
PsuI RGATCY 1 cut(s) 1477
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 2 cut(s) 326, 534
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 2 cut(s) 264, 493
SaqAI TTAA 8 cut(s) 230, 560, 771, 918, 981, 1128, 1308, 1406
SatI GCNGC 6 cut(s) 6, 22, 154, 177, 297, 347
Sau3AI GATC 6 cut(s) 163, 658, 790, 825, 1477, 1540
Sau96I GGNCC 4 cut(s) 136, 394, 1006, 1178
SchI GAGTC 4 cut(s) 87, 625, 1448, 1487
ScrFI CCNGG 3 cut(s) 65, 326, 744
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 5 cut(s) 613, 655, 931, 1028, 1334
SfcI CTRYAG 1 cut(s) 1473
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 2 cut(s) 136, 1178
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 2 cut(s) 326, 534
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 1325
Sse9I AATT 7 cut(s) 183, 523, 599, 753, 834, 881, 1221
SseBI AGGCCT 1 cut(s) 63
SsiI CCGC 8 cut(s) 8, 24, 154, 177, 296, 347, 1004, 1393
StuI AGGCCT 1 cut(s) 63
StyD4I CCNGG 3 cut(s) 63, 324, 742
StyI CCWWGG 2 cut(s) 290, 1227
TaaI ACNGT 5 cut(s) 379, 899, 1178, 1377, 1404
TaiI ACGT 2 cut(s) 1170, 1535
TaqI TCGA 5 cut(s) 126, 132, 1242, 1290, 1349
TaqII GACCGA 1 cut(s) 301
TasI AATT 7 cut(s) 183, 523, 599, 753, 834, 881, 1221
TatI WGTACW 1 cut(s) 42
TauI GCSGC 4 cut(s) 156, 179, 299, 349
TfiI GAWTC 2 cut(s) 202, 775
Tru1I TTAA 8 cut(s) 230, 560, 771, 918, 981, 1128, 1308, 1406
Tru9I TTAA 8 cut(s) 230, 560, 771, 918, 981, 1128, 1308, 1406
TscAI CASTG 2 cut(s) 1407, 1425
TseFI GTSAC 5 cut(s) 313, 893, 899, 1377, 1465
TseI GCWGC 2 cut(s) 5, 21
Tsp45I GTSAC 5 cut(s) 313, 893, 899, 1377, 1465
TspDTI ATGAA 6 cut(s) 158, 518, 894, 1050, 1218, 1287
TspGWI ACGGA 1 cut(s) 1505
TspRI CASTG 2 cut(s) 1407, 1425
Van91I CCANNNNNTGG 2 cut(s) 800, 1015
VpaK11BI GGWCC 2 cut(s) 136, 1178
XapI RAATTY 4 cut(s) 183, 523, 834, 881
XceI RCATGY 3 cut(s) 859, 1321, 1325
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 2 cut(s) 1248, 1251
Zsp2I ATGCAT 3 cut(s) 649, 1323, 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.