MD08G1035400.v1.1

Brassinazole-resistant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005623
Physical Location & Seq
Reverse (-)
2467157 .. 2468701
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1035400.v1.1.491

Sequence Viewer

Length: 888 bp
ATGACGTCGGATGGGGCGACTTCAGCGGCGACGATGGCGCGGAGGAAGCCGTCGTGGCGGGAGAGGGAGAACAACCGGAGGAGAGAGCGGAGGAGGAGAGCCATCGCGGCGAAGATATTCGCCGGCCTCCGAGCTCAGGGAGGCTTCAATTTGCCCAAGCACTGCGACAACAACGAGGTCCTTAAAGCTCTCTGCCTCCAGGCCGGCTGGACCGTCGAGGACGACGGCACCACCTACCGGAAGGGATTAAAGCCGAGTCAAACTGATACGCCGGCCGCATCAACCAAGATCAGCCCGTACTCTTCGCTAAATCCGAGTCCAATCCCGTCCTACCAAGTTAGTCCGTCGTCTTCGTCCTATCCCAGTCCCACCCGTTTCGATCCCGTCACTAATTCATCCAATCCAATTCACTATCTCCGCTCTGCAATCCCACCATCTCTCCCTCCCCTGCGAATTTCCAACAGTGCCCCTGTAACCCCGCCGCTCTCCTCCCCGACCTCCAGACGCCCCAACCCAATTCCCAACTGGGACACCATTGCCAAACAGTCCATGGCCTCTTTCGATTACCCATTTTACGCCGTCTCCGCTCCAGCGAGCCCGACCCGCCAACACCATCCTCATCCTACAGCTGCCACTATCCCCGAATGCGACGAGTCCGATGCTTCCACCGTCGATTCCGGACAATGGGTTTGCTTCCAGAGATTTGCTCCTTCTCTGTCAGCAATGCCAGCCTCTCCGACCTTCAGTCTTGTGAAGCCTGCTTTCCCTCAGCAGAATATTCCCGCGGATCAAATCCCGGACGTAAAGCCGTGGATCGGAGAGAAGATTCACGAGGTGGGATTGGATGACTTGGAGCTCACGCTTGGGAATGGAAAGGCTCGGATTTAA

Protein Analysis

296

Amino Acids

32.21

Weight (kDa)

9.43

Isoelectric Point (pI)

70.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BES1_N PF05687 13 - 133 1.3e-55 BES1/BZR1 plant transcription factor, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 4 cut(s) 88, 420, 484, 587
AccII CGCG 3 cut(s) 40, 107, 785
AccIII TCCGGA 1 cut(s) 677
AclWI GGATC 3 cut(s) 374, 795, 821
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 1 cut(s) 453
AcuI CTGAAG 2 cut(s) 6, 727
AcyI GRCGYC 2 cut(s) 5, 505
AdeI CACNNNGTG 1 cut(s) 835
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 4 cut(s) 136, 237, 684, 815
AgsI TTSAA 1 cut(s) 148
AhdI GACNNNNNGTC 1 cut(s) 744
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 4 cut(s) 134, 188, 629, 856
AluI AGCT 4 cut(s) 134, 188, 629, 856
Alw21I GWGCWC 2 cut(s) 136, 858
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 3 cut(s) 374, 795, 821
Aor13HI TCCGGA 1 cut(s) 677
AoxI GGCC 4 cut(s) 124, 201, 273, 552
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 1 cut(s) 453
ArsI GACNNNNNNTTYG 2 cut(s) 672, 704
Asp700I GAANNNNTTC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 178, 210
AsuC2I CCSGG 1 cut(s) 797
AvaII GGWCC 2 cut(s) 178, 210
BaeGI GKGCMC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 3 cut(s) 136, 599, 858
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 136, 858
BbvCI CCTCAGC 1 cut(s) 768
BbvI GCAGC 1 cut(s) 616
BccI CCATC 5 cut(s) 5, 28, 110, 442, 621
BceAI ACGGC 4 cut(s) 34, 241, 563, 793
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 1 cut(s) 200
BcnI CCSGG 1 cut(s) 797
BcoDI GTCTC 1 cut(s) 586
BfmI CTRYAG 1 cut(s) 624
BglI GCCNNNNNGGC 2 cut(s) 55, 107
BisI GCNGC 5 cut(s) 27, 108, 276, 482, 630
BlsI GCNGC 5 cut(s) 28, 109, 277, 483, 631
Bme1390I CCNGG 2 cut(s) 200, 797
Bme18I GGWCC 2 cut(s) 178, 210
BmeRI GACNNNNNGTC 1 cut(s) 744
BmgT120I GGNCC 2 cut(s) 178, 210
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 2 cut(s) 200, 797
BmrI ACTGGG 2 cut(s) 357, 535
BmsI GCATC 2 cut(s) 287, 649
BmuI ACTGGG 2 cut(s) 357, 535
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 691, 723
BpmI CTGGAG 3 cut(s) 182, 484, 573
Bpu10I CCTNAGC 2 cut(s) 135, 768
BpuMI CCSGG 1 cut(s) 797
BsaHI GRCGYC 2 cut(s) 5, 505
BsaJI CCNNGG 3 cut(s) 549, 783, 809
BsaWI WCCGGW 3 cut(s) 75, 237, 677
BsaXI ACNNNNNCTCC 4 cut(s) 423, 453, 566, 596
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 237, 684, 815
Bse118I RCCGGY 3 cut(s) 122, 203, 271
Bse1I ACTGG 2 cut(s) 363, 530
Bse3DI GCAATG 2 cut(s) 534, 729
BseAI TCCGGA 1 cut(s) 677
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 3 cut(s) 549, 783, 809
BseGI GGATG 5 cut(s) 16, 395, 613, 619, 850
BseLI CCNNNNNNNGG 4 cut(s) 136, 237, 684, 815
BseMI GCAATG 2 cut(s) 534, 729
BseMII CTCAG 2 cut(s) 149, 782
BseNI ACTGG 2 cut(s) 363, 530
BseRI GAGGAG 4 cut(s) 94, 106, 109, 478
BseSI GKGCMC 1 cut(s) 469
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 1 cut(s) 616
Bsh1236I CGCG 3 cut(s) 40, 107, 785
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 126, 203, 275, 554
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 136, 858
BsiSI CCGG 7 cut(s) 76, 123, 204, 238, 272, 678, 797
BslFI GGGAC 2 cut(s) 351, 542
BslI CCNNNNNNNGG 4 cut(s) 136, 237, 684, 815
BsmAI GTCTC 1 cut(s) 586
BsmBI CGTCTC 1 cut(s) 586
BsmFI GGGAC 2 cut(s) 351, 542
BsmI GAATGC 1 cut(s) 650
BsnI GGCC 4 cut(s) 126, 203, 275, 554
Bsp1286I GDGCHC 4 cut(s) 136, 469, 599, 858
Bsp13I TCCGGA 1 cut(s) 677
Bsp143I GATC 4 cut(s) 288, 379, 787, 813
Bsp19I CCATGG 1 cut(s) 549
BspANI GGCC 4 cut(s) 126, 203, 275, 554
BspCNI CTCAG 2 cut(s) 148, 781
BspEI TCCGGA 1 cut(s) 677
BspFNI CGCG 3 cut(s) 40, 107, 785
BspLI GGNNCC 1 cut(s) 229
BspPI GGATC 3 cut(s) 374, 795, 821
BspT107I GGYRCC 1 cut(s) 227
BsrBI CCGCTC 4 cut(s) 88, 420, 484, 587
BsrDI GCAATG 2 cut(s) 534, 729
BsrFI RCCGGY 3 cut(s) 122, 203, 271
BsrI ACTGG 2 cut(s) 363, 530
BssAI RCCGGY 3 cut(s) 122, 203, 271
BssECI CCNNGG 3 cut(s) 549, 783, 809
BssMI GATC 4 cut(s) 288, 379, 787, 813
BssNI GRCGYC 2 cut(s) 5, 505
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 4 cut(s) 214, 464, 546, 670
Bst6I CTCTTC 1 cut(s) 307
BstACI GRCGYC 2 cut(s) 5, 505
BstC8I GCNNGC 6 cut(s) 124, 205, 273, 595, 729, 759
BstDEI CTNAG 2 cut(s) 135, 768
BstDSI CCRYGG 3 cut(s) 549, 783, 809
BstF5I GGATG 5 cut(s) 16, 395, 613, 619, 850
BstFNI CGCG 3 cut(s) 40, 107, 785
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 291, 382, 790, 816
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 4 cut(s) 288, 379, 787, 813
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 8 cut(s) 23, 35, 46, 55, 107, 584, 603, 728
BstNI CCWGG 1 cut(s) 200
BstSCI CCNGG 2 cut(s) 198, 795
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 469
BstUI CGCG 3 cut(s) 40, 107, 785
BstV1I GCAGC 1 cut(s) 616
BstV2I GAAGAC 1 cut(s) 342
BstZI CGGCCG 1 cut(s) 273
BsuRI GGCC 4 cut(s) 126, 203, 275, 554
BtgI CCRYGG 3 cut(s) 549, 783, 809
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 5 cut(s) 16, 395, 613, 619, 850
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 160, 469
Cac8I GCNNGC 6 cut(s) 124, 205, 273, 595, 729, 759
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 3 cut(s) 122, 203, 271
Cfr13I GGNCC 2 cut(s) 178, 210
Cfr42I CCGCGG 1 cut(s) 786
CseI GACGC 1 cut(s) 513
Csp6I GTAC 1 cut(s) 298
CviAII CATG 1 cut(s) 550
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 2 cut(s) 135, 768
DpnI GATC 4 cut(s) 290, 381, 789, 815
DpnII GATC 4 cut(s) 288, 379, 787, 813
DraIII CACNNNGTG 1 cut(s) 835
DriI GACNNNNNGTC 1 cut(s) 744
EaeI YGGCCR 1 cut(s) 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 307
Eam1105I GACNNNNNGTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 307
Ecl136II GAGCTC 2 cut(s) 134, 856
EclXI CGGCCG 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 549
Eco24I GRGCYC 3 cut(s) 136, 599, 858
Eco47I GGWCC 2 cut(s) 178, 210
Eco52I CGGCCG 1 cut(s) 273
Eco53kI GAGCTC 2 cut(s) 134, 856
Eco57I CTGAAG 2 cut(s) 6, 727
EcoICRI GAGCTC 2 cut(s) 134, 856
EcoO109I RGGNCCY 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 3 cut(s) 136, 599, 858
ErhI CCWWGG 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 586
FaeI CATG 1 cut(s) 553
FaiI YATR 1 cut(s) 551
FaqI GGGAC 2 cut(s) 351, 542
FatI CATG 1 cut(s) 549
FauI CCCGC 4 cut(s) 51, 486, 611, 790
Fnu4HI GCNGC 5 cut(s) 27, 108, 276, 482, 630
FokI GGATG 5 cut(s) 23, 382, 600, 606, 857
FriOI GRGCYC 3 cut(s) 136, 599, 858
Fsp4HI GCNGC 5 cut(s) 27, 108, 276, 482, 630
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 27, 108, 276, 482, 630
GsuI CTGGAG 3 cut(s) 182, 484, 573
HaeIII GGCC 4 cut(s) 126, 203, 275, 554
HapII CCGG 7 cut(s) 76, 123, 204, 238, 272, 678, 797
HgaI GACGC 1 cut(s) 513
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 5, 505
Hin1II CATG 1 cut(s) 553
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 5 cut(s) 256, 316, 653, 674, 826
HpaII CCGG 7 cut(s) 76, 123, 204, 238, 272, 678, 797
Hpy188I TCNGA 7 cut(s) 10, 131, 315, 658, 738, 818, 882
Hpy188III TCNNGA 4 cut(s) 501, 678, 697, 830
Hpy99I CGWCG 8 cut(s) 10, 34, 55, 218, 227, 349, 653, 674
HpyAV CCTTC 3 cut(s) 235, 720, 751
HpyCH4III ACNGT 4 cut(s) 214, 464, 546, 670
HpyCH4IV ACGT 2 cut(s) 5, 801
HpyCH4V TGCA 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 35, 46, 55, 107, 584, 603, 728
HpyF3I CTNAG 2 cut(s) 135, 768
HpySE526I ACGT 2 cut(s) 5, 801
Hsp92I GRCGYC 2 cut(s) 5, 505
Hsp92II CATG 1 cut(s) 553
HspAI GCGC 1 cut(s) 38
Kpn2I TCCGGA 1 cut(s) 677
KroI GCCGGC 3 cut(s) 122, 203, 271
KroNI GCCGGC 3 cut(s) 124, 205, 273
KspI CCGCGG 1 cut(s) 786
Kzo9I GATC 4 cut(s) 288, 379, 787, 813
LmnI GCTCC 3 cut(s) 592, 712, 853
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 2 cut(s) 287, 649
MaeII ACGT 2 cut(s) 5, 801
MaeIII GTNAC 2 cut(s) 385, 472
MalI GATC 4 cut(s) 290, 381, 789, 815
MbiI CCGCTC 4 cut(s) 88, 420, 484, 587
MboI GATC 4 cut(s) 288, 379, 787, 813
MboII GAAGA 4 cut(s) 124, 294, 342, 835
MhlI GDGCHC 4 cut(s) 136, 469, 599, 858
MluCI AATT 5 cut(s) 148, 391, 405, 453, 516
MlyI GAGTC 3 cut(s) 265, 325, 662
MmeI TCCRAC 2 cut(s) 483, 761
MroI TCCGGA 1 cut(s) 677
MroNI GCCGGC 3 cut(s) 122, 203, 271
MroXI GAANNNNTTC 1 cut(s) 116
MseI TTAA 3 cut(s) 183, 248, 886
MspA1I CMGCKG 3 cut(s) 26, 629, 785
MspI CCGG 7 cut(s) 76, 123, 204, 238, 272, 678, 797
MspR9I CCNGG 2 cut(s) 200, 797
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 3 cut(s) 40, 107, 785
MwoI GCNNNNNNNGC 8 cut(s) 23, 35, 46, 55, 107, 584, 603, 728
NaeI GCCGGC 3 cut(s) 124, 205, 273
NciI CCSGG 1 cut(s) 797
NcoI CCATGG 1 cut(s) 549
NdeII GATC 4 cut(s) 288, 379, 787, 813
NgoMIV GCCGGC 3 cut(s) 122, 203, 271
NlaIII CATG 1 cut(s) 553
NlaIV GGNNCC 1 cut(s) 229
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 385
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 311
PctI GAATGC 1 cut(s) 650
PdiI GCCGGC 3 cut(s) 124, 205, 273
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 674, 826
PfoI TCCNGGA 1 cut(s) 795
PkrI GCNGC 5 cut(s) 28, 109, 277, 483, 631
PleI GAGTC 3 cut(s) 264, 324, 661
PpsI GAGTC 3 cut(s) 264, 324, 661
PpuMI RGGWCCY 1 cut(s) 178
Psp124BI GAGCTC 2 cut(s) 136, 858
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 229
PspPI GGNCC 2 cut(s) 178, 210
PspPPI RGGWCCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 629
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SacI GAGCTC 2 cut(s) 136, 858
SacII CCGCGG 1 cut(s) 786
SaqAI TTAA 3 cut(s) 183, 248, 886
SatI GCNGC 5 cut(s) 27, 108, 276, 482, 630
Sau3AI GATC 4 cut(s) 288, 379, 787, 813
Sau96I GGNCC 2 cut(s) 178, 210
SchI GAGTC 3 cut(s) 265, 325, 662
ScrFI CCNGG 2 cut(s) 200, 797
SduI GDGCHC 4 cut(s) 136, 469, 599, 858
SfaNI GCATC 2 cut(s) 287, 649
SfcI CTRYAG 1 cut(s) 624
Sfr303I CCGCGG 1 cut(s) 786
SgrBI CCGCGG 1 cut(s) 786
SinI GGWCC 2 cut(s) 178, 210
Sse9I AATT 5 cut(s) 148, 391, 405, 453, 516
SspI AATATT 1 cut(s) 778
SstI GAGCTC 2 cut(s) 136, 858
StyD4I CCNGG 2 cut(s) 198, 795
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 214, 464, 546, 670
TaiI ACGT 2 cut(s) 8, 804
TaqI TCGA 4 cut(s) 216, 378, 561, 672
TasI AATT 5 cut(s) 148, 391, 405, 453, 516
TauI GCSGC 4 cut(s) 29, 110, 278, 484
TfiI GAWTC 2 cut(s) 674, 826
Tru1I TTAA 3 cut(s) 183, 248, 886
Tru9I TTAA 3 cut(s) 183, 248, 886
TscAI CASTG 2 cut(s) 167, 469
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 2 cut(s) 167, 469
VpaK11BI GGWCC 2 cut(s) 178, 210
XapI RAATTY 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 2 cut(s) 522, 547
XmnI GAANNNNTTC 1 cut(s) 116
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.