MD08G1040100.v1.1

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012880
Physical Location & Seq
Forward (+)
2966148 .. 2966909
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1040100.v1.1.491

Sequence Viewer

Length: 762 bp
ATGCCGTTTGACTCTGCCGACGCGCAAATGCGACAGTCGGCGACGACGAGAGTCCAGCACAGTCTGGGTCACCCACCGCCGCAGCAGACCGAGCCACTTCACTGCCCCCGTTGCGATTCCGACAACACCAAGTTCTGCTACTACAACAATTATAACCTCGCCCAGCCACGCCACTTCTGCAAGTCCTGCCGCCGCTACTGGACCCACGGTGGCACCCTCCGAAATGTCCCCGTCGGCGGTGGCTCCCGCAAGAACACCAAGCGTTCTCGCCCGTCCTGCTCTTCCTCCTCAGGCTCGCCCTCCTCCTCTTCACTGACCCAGGAGGTCAGCTCCGTCGCTCCCACAAAACCCATTTCCTTTCCAGTGGAACCGAAGCTGGAGACGGCGTTGGATCACGACAAACTAATGGACCTGAACGAAAGCGCTGTCGGGAACGGCGGCTTCACTTCTATGTTGAGTTCTCAGGGCCCACCTGCACCTGGGTTCTTGGCCCTTGGCGGGTTTGGGTATGGATTCGGGTACGGGTTGGGACTCGGGCAAGGGTTGGACGAAGTGGGTACTTTCGGCTACGGCGGCGGCAGAGGCGTCCTCGCTTTTCCCGAGGAAGGTGACTTTGCAAATATTAACCACAACAACAGTGGAGCTGTTTCTGGGGGTTCTGTCGGGTGCAACACGTGGCAGATTATGACTGGTGGTGATGGATGGATGGCCGAGAACAACGGGGATTGCTTTACCTGGCCGGAGCTTGAGATTTCCAAGTAA

Protein Analysis

254

Amino Acids

26.91

Weight (kDa)

6.15

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 32 - 87 7.1e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47655
fragaria_vesca FvH4_2g40660
malus_domestica MD08G1040100.v1.1 MD15G1034500.v1.1
prunus_persica Prupe.1G388600_v2.0.a1 Prupe.1G388600_v2.0.a1
pyrus_communis pycom08g03230 pycom15g03180
rosa_chinensis RchiOBHm_Chr6g0300001
rosa_laevigata RLG00000011373
rosa_multiflora Rmu_sc0001370.1_g000002 Rmu_sc0011230.1_g000001
rosa_roxburghii Rroxscaffold_7G00168210
rosa_rugosa Rorug06G0293400
rosa_samantha Rh6AG405100 Rh6BG413400 Rh6CG420500 Rh6DG406100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 153
AarI CACCTGC 1 cut(s) 481
Acc36I ACCTGC 1 cut(s) 481
AccB1I GGYRCC 1 cut(s) 212
AccII CGCG 1 cut(s) 23
AclWI GGATC 1 cut(s) 399
AcoI YGGCCR 2 cut(s) 708, 737
AcvI CACGTG 1 cut(s) 675
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 2 cut(s) 521, 559
AfeI AGCGCT 1 cut(s) 424
AfiI CCNNNNNNNGG 4 cut(s) 236, 479, 498, 605
AflIII ACRYGT 1 cut(s) 672
AjnI CCWGG 3 cut(s) 318, 478, 734
AluBI AGCT 4 cut(s) 330, 376, 644, 745
AluI AGCT 4 cut(s) 330, 376, 644, 745
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 1 cut(s) 399
Ama87I CYCGRG 2 cut(s) 533, 599
Aor51HI AGCGCT 1 cut(s) 424
AoxI GGCC 4 cut(s) 466, 489, 708, 737
ApaI GGGCCC 1 cut(s) 470
ApeKI GCWGC 1 cut(s) 82
ArsI GACNNNNNNTTYG 2 cut(s) 411, 443
AspLEI GCGC 2 cut(s) 25, 425
AspS9I GGNCC 5 cut(s) 201, 409, 466, 467, 490
AsuHPI GGTGA 3 cut(s) 62, 620, 707
AvaI CYCGRG 2 cut(s) 533, 599
AvaII GGWCC 2 cut(s) 201, 409
AxyI CCTNAGG 1 cut(s) 289
BaeGI GKGCMC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 470
BbrPI CACGTG 1 cut(s) 675
BbvI GCAGC 1 cut(s) 94
BccI CCATC 3 cut(s) 692, 696, 700
BceAI ACGGC 3 cut(s) 399, 451, 586
BciT130I CCWGG 3 cut(s) 320, 480, 736
BcoDI GTCTC 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 481
BisI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
BlsI GCNGC 7 cut(s) 81, 84, 191, 194, 440, 575, 578
Bme1390I CCNGG 3 cut(s) 320, 480, 736
Bme18I GGWCC 2 cut(s) 201, 409
BmeT110I CYCGRG 2 cut(s) 533, 599
BmgT120I GGNCC 5 cut(s) 201, 409, 466, 467, 490
BmiI GGNNCC 5 cut(s) 203, 214, 244, 369, 468
BmrFI CCNGG 3 cut(s) 320, 480, 736
BoxI GACNNNNGTC 1 cut(s) 50
BplI GAGNNNNNCTC 4 cut(s) 314, 346, 573, 605
BpmI CTGGAG 1 cut(s) 398
BsaAI YACGTR 1 cut(s) 675
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 5 cut(s) 205, 318, 479, 493, 600
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 479, 498, 605
Bse1I ACTGG 3 cut(s) 203, 362, 694
Bse21I CCTNAGG 1 cut(s) 289
BseBI CCWGG 3 cut(s) 320, 480, 736
BseDI CCNNGG 5 cut(s) 205, 318, 479, 493, 600
BseGI GGATG 2 cut(s) 707, 711
BseLI CCNNNNNNNGG 4 cut(s) 236, 479, 498, 605
BseMII CTCAG 2 cut(s) 303, 476
BseNI ACTGG 3 cut(s) 203, 362, 694
BseRI GAGGAG 3 cut(s) 277, 292, 295
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 1 cut(s) 94
BseYI CCCAGC 1 cut(s) 162
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 4 cut(s) 468, 491, 710, 739
BshNI GGYRCC 1 cut(s) 212
BsiHKCI CYCGRG 2 cut(s) 533, 599
BsiSI CCGG 1 cut(s) 740
BslFI GGGAC 2 cut(s) 212, 543
BslI CCNNNNNNNGG 4 cut(s) 236, 479, 498, 605
BsmAI GTCTC 1 cut(s) 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 212, 543
BsnI GGCC 4 cut(s) 468, 491, 710, 739
BsoBI CYCGRG 2 cut(s) 533, 599
Bsp120I GGGCCC 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 1 cut(s) 391
BspANI GGCC 4 cut(s) 468, 491, 710, 739
BspCNI CTCAG 2 cut(s) 302, 475
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 5 cut(s) 203, 214, 244, 369, 468
BspMI ACCTGC 1 cut(s) 481
BspPI GGATC 1 cut(s) 399
BspQI GCTCTTC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 212
BsrI ACTGG 3 cut(s) 203, 362, 694
BssECI CCNNGG 5 cut(s) 205, 318, 479, 493, 600
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 3 cut(s) 320, 480, 736
Bst4CI ACNGT 4 cut(s) 36, 62, 209, 638
Bst6I CTCTTC 2 cut(s) 286, 313
BstACI GRCGYC 1 cut(s) 585
BstAPI GCANNNNNTGC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 675
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 2 cut(s) 289, 462
BstDSI CCRYGG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 2 cut(s) 707, 711
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 25, 425
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 276, 573, 582
BstNI CCWGG 3 cut(s) 320, 480, 736
BstPAI GACNNNNGTC 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 3 cut(s) 318, 478, 734
BstSLI GKGCMC 1 cut(s) 470
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 94
Bsu36I CCTNAGG 1 cut(s) 289
BsuRI GGCC 4 cut(s) 468, 491, 710, 739
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 707, 711
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 4 cut(s) 100, 311, 369, 643
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 296
CfoI GCGC 2 cut(s) 25, 425
Cfr13I GGNCC 5 cut(s) 201, 409, 466, 467, 490
CseI GACGC 2 cut(s) 29, 574
Csp6I GTAC 2 cut(s) 520, 558
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
CviQI GTAC 2 cut(s) 520, 558
DdeI CTNAG 2 cut(s) 289, 462
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 708, 737
Eam1104I CTCTTC 2 cut(s) 286, 313
EarI CTCTTC 2 cut(s) 286, 313
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 201, 409
Eco47III AGCGCT 1 cut(s) 424
Eco72I CACGTG 1 cut(s) 675
Eco81I CCTNAGG 1 cut(s) 289
Eco88I CYCGRG 2 cut(s) 533, 599
Eco91I GGTNACC 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 466
EcoO65I GGTNACC 1 cut(s) 68
EcoRII CCWGG 3 cut(s) 318, 478, 734
EcoT14I CCWWGG 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 374
FaiI YATR 4 cut(s) 153, 452, 510, 686
FaqI GGGAC 2 cut(s) 212, 543
FauI CCCGC 2 cut(s) 254, 491
Fnu4HI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
FokI GGATG 2 cut(s) 714, 718
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
GlaI GCGC 2 cut(s) 24, 424
GluI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 4 cut(s) 468, 491, 710, 739
HapII CCGG 1 cut(s) 740
HgaI GACGC 2 cut(s) 29, 574
HhaI GCGC 2 cut(s) 25, 425
Hin1I GRCGYC 1 cut(s) 585
Hin6I GCGC 2 cut(s) 23, 423
HinP1I GCGC 2 cut(s) 23, 423
HinfI GANTC 5 cut(s) 11, 51, 116, 513, 531
HpaII CCGG 1 cut(s) 740
HphI GGTGA 3 cut(s) 62, 620, 707
Hpy188I TCNGA 2 cut(s) 121, 221
Hpy188III TCNNGA 3 cut(s) 395, 430, 599
Hpy99I CGWCG 5 cut(s) 23, 46, 49, 236, 338
HpyAV CCTTC 1 cut(s) 599
HpyCH4III ACNGT 4 cut(s) 36, 62, 209, 638
HpyCH4IV ACGT 1 cut(s) 674
HpyCH4V TGCA 4 cut(s) 180, 476, 617, 669
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 276, 573, 582
HpyF3I CTNAG 2 cut(s) 289, 462
HpySE526I ACGT 1 cut(s) 674
Hsp92I GRCGYC 1 cut(s) 585
HspAI GCGC 2 cut(s) 23, 423
Kzo9I GATC 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 5 cut(s) 248, 335, 343, 641, 742
Lsp1109I GCAGC 1 cut(s) 94
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 2 cut(s) 68, 608
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 2 cut(s) 273, 300
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 1 cut(s) 148
MlyI GAGTC 3 cut(s) 5, 60, 525
MmeI TCCRAC 3 cut(s) 144, 369, 525
MseI TTAA 1 cut(s) 624
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 1 cut(s) 740
MspR9I CCNGG 3 cut(s) 320, 480, 736
MvaI CCWGG 3 cut(s) 320, 480, 736
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 276, 573, 582
NdeII GATC 1 cut(s) 391
NlaIV GGNNCC 5 cut(s) 203, 214, 244, 369, 468
NmeAIII GCCGAG 1 cut(s) 736
NmuCI GTSAC 2 cut(s) 68, 608
PaqCI CACCTGC 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 597
PfeI GAWTC 2 cut(s) 116, 513
PkrI GCNGC 7 cut(s) 81, 84, 191, 194, 440, 575, 578
PleI GAGTC 3 cut(s) 5, 59, 525
PmaCI CACGTG 1 cut(s) 675
PmlI CACGTG 1 cut(s) 675
PpsI GAGTC 3 cut(s) 5, 59, 525
Ppu21I YACGTR 1 cut(s) 675
PshAI GACNNNNGTC 1 cut(s) 50
PsiI TTATAA 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 318, 478, 734
PspCI CACGTG 1 cut(s) 675
PspEI GGTNACC 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 3 cut(s) 318, 478, 734
PspN4I GGNNCC 5 cut(s) 203, 214, 244, 369, 468
PspOMI GGGCCC 1 cut(s) 466
PspPI GGNCC 5 cut(s) 201, 409, 466, 467, 490
RsaI GTAC 2 cut(s) 521, 559
RsaNI GTAC 2 cut(s) 520, 558
RseI CAYNNNNRTG 1 cut(s) 449
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 5 cut(s) 201, 409, 466, 467, 490
SchI GAGTC 3 cut(s) 5, 60, 525
ScrFI CCNGG 3 cut(s) 320, 480, 736
SduI GDGCHC 1 cut(s) 470
SinI GGWCC 2 cut(s) 201, 409
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
Sse9I AATT 1 cut(s) 148
SspI AATATT 1 cut(s) 622
StyD4I CCNGG 3 cut(s) 318, 478, 734
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 4 cut(s) 36, 62, 209, 638
TaiI ACGT 1 cut(s) 677
TaqII GACCGA 1 cut(s) 104
TasI AATT 1 cut(s) 148
TauI GCSGC 6 cut(s) 82, 192, 195, 441, 576, 579
TfiI GAWTC 2 cut(s) 116, 513
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 4 cut(s) 107, 318, 369, 643
TseFI GTSAC 2 cut(s) 68, 608
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 2 cut(s) 68, 608
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 4 cut(s) 107, 318, 369, 643
VpaK11BI GGWCC 2 cut(s) 201, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.