pycom15g03180

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012880
Physical Location & Seq
Forward (+)
1990222 .. 1991358
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03180.1

Sequence Viewer

Length: 831 bp
ATGACAAGAACAAGGTTGAGTAGATCGATCACGCTAACCCTACCTGATACGCCAGCTTCCCCACAAGACACACCACAAAAATATCATTATCCCCCGCACCATCAATCTCAAATGCGACCGTCGATGACGACGAGAGTCCAGCACAGCCTGGGCCACCCTCCGCCGCAGCAGACCGAGCCGCTTCACTGCCCCCGCTGCGATTCTGACAACACCAAGTTCTGCTACTACAACAATTACAACCTCGCCCAGCCACGCCACTTCTGCAAGTCCTGCCGCCGGTACTGGACCCACGGCGGAACCCTCCGCAATGTCCCAGTCGGCGGCGGGTCTCGCAAGAACACCAAGCGTTCTCGCCCATCCTGCTCTTCCTCCTCGGGCTCGCCCTCCTCCTCTTCACTGACCCAGGAGGTCAGCTCCGTCGCGTCCGCTAAACCCAATTCCTTTCCGGTGGAACCGAAGCCTGAGATGGCGACGGATCACGAAAAACTGATGGACCTGAACGAGAGCGCTGTCGGAAACGGGGGCTTCACTTCTCTGTTGAGTGCTCAGGGCCCACCTGCACCTGGGTTCTTGGCGTTTAGCGGGTTCGGGTACGGGTTGGGGCTCGGGCAAGGGTTTGACGAAGTGGGTACTTACGGCTACGACGGCGGCAGAGGTGTGCTCGCTTTTCCCGACGTGGGTGATTTTGGAAATGTTATCCACAACAATGGAGCTGTTTCTGGGGGTTCTGTCGGGTGCAACACGTGGCAGATTATGACTGGTGGTGATGGTGGGATAGCCGAGAACAATGGTGATTGCTTTGCCTGGCCGGAGCTGGAGACTTCCAAGTAA

Protein Analysis

277

Amino Acids

29.59

Weight (kDa)

7.64

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 60 - 115 8.3e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47655
fragaria_vesca FvH4_2g40660
malus_domestica MD08G1040100.v1.1 MD15G1034500.v1.1
prunus_persica Prupe.1G388600_v2.0.a1 Prupe.1G388600_v2.0.a1
pyrus_communis pycom08g03230 pycom15g03180
rosa_chinensis RchiOBHm_Chr6g0300001
rosa_laevigata RLG00000011373
rosa_multiflora Rmu_sc0001370.1_g000002 Rmu_sc0011230.1_g000001
rosa_roxburghii Rroxscaffold_7G00168210
rosa_rugosa Rorug06G0293400
rosa_samantha Rh6AG405100 Rh6BG413400 Rh6CG420500 Rh6DG406100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 565
Acc36I ACCTGC 1 cut(s) 565
AccII CGCG 1 cut(s) 422
AclWI GGATC 1 cut(s) 483
AcoI YGGCCR 1 cut(s) 806
AcvI CACGTG 1 cut(s) 744
AfaI GTAC 3 cut(s) 281, 593, 631
AfeI AGCGCT 1 cut(s) 508
AfiI CCNNNNNNNGG 4 cut(s) 276, 320, 563, 677
AflIII ACRYGT 1 cut(s) 741
AjiI CACGTC 1 cut(s) 676
AjnI CCWGG 4 cut(s) 147, 402, 562, 803
AluBI AGCT 4 cut(s) 56, 414, 713, 814
AluI AGCT 4 cut(s) 56, 414, 713, 814
Alw21I GWGCWC 2 cut(s) 547, 663
Alw26I GTCTC 2 cut(s) 333, 812
AlwI GGATC 1 cut(s) 483
Ama87I CYCGRG 2 cut(s) 373, 605
Aor51HI AGCGCT 1 cut(s) 508
AoxI GGCC 3 cut(s) 151, 550, 806
ApaI GGGCCC 1 cut(s) 554
ApeKI GCWGC 2 cut(s) 166, 195
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 5 cut(s) 151, 285, 493, 550, 551
AsuHPI GGTGA 3 cut(s) 692, 776, 803
AvaI CYCGRG 2 cut(s) 373, 605
AvaII GGWCC 2 cut(s) 285, 493
BaeGI GKGCMC 1 cut(s) 554
BanII GRGCYC 3 cut(s) 380, 554, 606
BbrPI CACGTG 1 cut(s) 744
Bbv12I GWGCWC 2 cut(s) 547, 663
BbvI GCAGC 2 cut(s) 178, 182
BccI CCATC 5 cut(s) 108, 364, 460, 484, 761
BceAI ACGGC 3 cut(s) 307, 652, 661
BciT130I CCWGG 4 cut(s) 149, 404, 564, 805
BcoDI GTCTC 2 cut(s) 333, 812
BfoI RGCGCY 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 7 cut(s) 164, 167, 179, 196, 274, 322, 649
BlsI GCNGC 7 cut(s) 165, 168, 180, 197, 275, 323, 650
Bme1390I CCNGG 4 cut(s) 149, 404, 564, 805
Bme18I GGWCC 2 cut(s) 285, 493
BmeT110I CYCGRG 2 cut(s) 373, 605
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 5 cut(s) 151, 285, 493, 550, 551
BmiI GGNNCC 4 cut(s) 287, 298, 453, 552
BmrFI CCNGG 4 cut(s) 149, 404, 564, 805
BmrI ACTGGG 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 308
BoxI GACNNNNGTC 1 cut(s) 134
BplI GAGNNNNNCTC 4 cut(s) 398, 430, 645, 677
Bpu10I CCTNAGC 1 cut(s) 546
Bsa29I ATCGAT 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 5 cut(s) 148, 289, 372, 402, 563
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 320, 563, 677
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 3 cut(s) 287, 314, 763
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 4 cut(s) 149, 404, 564, 805
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 5 cut(s) 148, 289, 372, 402, 563
BseGI GGATG 1 cut(s) 356
BseLI CCNNNNNNNGG 4 cut(s) 276, 320, 563, 677
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 2 cut(s) 453, 560
BseNI ACTGG 3 cut(s) 287, 314, 763
BseRI GAGGAG 3 cut(s) 361, 376, 379
BseSI GKGCMC 1 cut(s) 554
BseXI GCAGC 2 cut(s) 178, 182
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 1 cut(s) 422
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 153, 552, 808
BshVI ATCGAT 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 547, 663
BsiHKCI CYCGRG 2 cut(s) 373, 605
BsiSI CCGG 3 cut(s) 277, 446, 809
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 4 cut(s) 276, 320, 563, 677
BsmAI GTCTC 2 cut(s) 333, 812
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 3 cut(s) 153, 552, 808
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 2 cut(s) 373, 605
Bsp120I GGGCCC 1 cut(s) 550
Bsp1286I GDGCHC 5 cut(s) 380, 547, 554, 606, 663
Bsp143I GATC 3 cut(s) 23, 27, 475
BspANI GGCC 3 cut(s) 153, 552, 808
BspCNI CTCAG 2 cut(s) 454, 559
BspDI ATCGAT 1 cut(s) 26
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 4 cut(s) 287, 298, 453, 552
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 1 cut(s) 483
BspQI GCTCTTC 1 cut(s) 370
BspTNI GGTCTC 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 276
BsrI ACTGG 3 cut(s) 287, 314, 763
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 5 cut(s) 148, 289, 372, 402, 563
BssMI GATC 3 cut(s) 23, 27, 475
Bst2UI CCWGG 4 cut(s) 149, 404, 564, 805
Bst4CI ACNGT 1 cut(s) 120
Bst6I CTCTTC 2 cut(s) 370, 397
BstAPI GCANNNNNTGC 1 cut(s) 270
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 3 cut(s) 54, 380, 663
BstDEI CTNAG 2 cut(s) 462, 546
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 1 cut(s) 356
BstFNI CGCG 1 cut(s) 422
BstH2I RGCGCY 1 cut(s) 510
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 3 cut(s) 26, 30, 478
BstMAI GTCTC 2 cut(s) 333, 812
BstMBI GATC 3 cut(s) 23, 27, 475
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 7 cut(s) 175, 195, 261, 270, 330, 360, 645
BstNI CCWGG 4 cut(s) 149, 404, 564, 805
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 147, 402, 562, 803
BstSLI GKGCMC 1 cut(s) 554
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 2 cut(s) 178, 182
BstXI CCANNNNNNTGG 1 cut(s) 707
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 3 cut(s) 153, 552, 808
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 289
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 1 cut(s) 356
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 184, 395
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 3 cut(s) 54, 380, 663
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 5 cut(s) 151, 285, 493, 550, 551
ClaI ATCGAT 1 cut(s) 26
CseI GACGC 1 cut(s) 411
Csp6I GTAC 3 cut(s) 280, 592, 630
CviQI GTAC 3 cut(s) 280, 592, 630
DdeI CTNAG 2 cut(s) 462, 546
DpnI GATC 3 cut(s) 25, 29, 477
DpnII GATC 3 cut(s) 23, 27, 475
EaeI YGGCCR 1 cut(s) 806
Eam1104I CTCTTC 2 cut(s) 370, 397
EarI CTCTTC 2 cut(s) 370, 397
EciI GGCGGA 2 cut(s) 150, 309
Eco24I GRGCYC 3 cut(s) 380, 554, 606
Eco31I GGTCTC 1 cut(s) 333
Eco47I GGWCC 2 cut(s) 285, 493
Eco47III AGCGCT 1 cut(s) 508
Eco72I CACGTG 1 cut(s) 744
Eco88I CYCGRG 2 cut(s) 373, 605
EcoO109I RGGNCCY 1 cut(s) 550
EcoRII CCWGG 4 cut(s) 147, 402, 562, 803
EcoT38I GRGCYC 3 cut(s) 380, 554, 606
FaiI YATR 1 cut(s) 755
FaqI GGGAC 1 cut(s) 296
FauI CCCGC 4 cut(s) 102, 200, 317, 575
Fnu4HI GCNGC 7 cut(s) 164, 167, 179, 196, 274, 322, 649
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 3 cut(s) 380, 554, 606
Fsp4HI GCNGC 7 cut(s) 164, 167, 179, 196, 274, 322, 649
GlaI GCGC 1 cut(s) 508
GluI GCNGC 7 cut(s) 164, 167, 179, 196, 274, 322, 649
GsaI CCCAGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 510
HaeIII GGCC 3 cut(s) 153, 552, 808
HapII CCGG 3 cut(s) 277, 446, 809
HgaI GACGC 1 cut(s) 411
HhaI GCGC 1 cut(s) 509
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HinfI GANTC 2 cut(s) 135, 200
HpaII CCGG 3 cut(s) 277, 446, 809
HphI GGTGA 3 cut(s) 692, 776, 803
Hpy188I TCNGA 2 cut(s) 205, 515
Hpy188III TCNNGA 2 cut(s) 479, 671
Hpy99I CGWCG 6 cut(s) 124, 133, 422, 475, 647, 677
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4IV ACGT 2 cut(s) 675, 743
HpyCH4V TGCA 3 cut(s) 264, 560, 738
HpyF10VI GCNNNNNNNGC 7 cut(s) 175, 195, 261, 270, 330, 360, 645
HpyF3I CTNAG 2 cut(s) 462, 546
HpySE526I ACGT 2 cut(s) 675, 743
HspAI GCGC 1 cut(s) 507
Kzo9I GATC 3 cut(s) 23, 27, 475
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 3 cut(s) 419, 710, 811
Lsp1109I GCAGC 2 cut(s) 178, 182
MaeII ACGT 2 cut(s) 675, 743
MalI GATC 3 cut(s) 25, 29, 477
MboI GATC 3 cut(s) 23, 27, 475
MboII GAAGA 2 cut(s) 357, 384
MhlI GDGCHC 5 cut(s) 380, 547, 554, 606, 663
MluCI AATT 2 cut(s) 232, 436
MlyI GAGTC 1 cut(s) 144
MmeI TCCRAC 1 cut(s) 493
MslI CAYNNNNRTG 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 3 cut(s) 277, 446, 809
MspR9I CCNGG 4 cut(s) 149, 404, 564, 805
MvaI CCWGG 4 cut(s) 149, 404, 564, 805
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 7 cut(s) 175, 195, 261, 270, 330, 360, 645
NdeII GATC 3 cut(s) 23, 27, 475
NlaIV GGNNCC 4 cut(s) 287, 298, 453, 552
NmeAIII GCCGAG 1 cut(s) 805
PaqCI CACCTGC 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 669
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 7 cut(s) 165, 168, 180, 197, 275, 323, 650
PleI GAGTC 1 cut(s) 143
PmaCI CACGTG 1 cut(s) 744
PmlI CACGTG 1 cut(s) 744
PpsI GAGTC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 744
PshAI GACNNNNGTC 1 cut(s) 134
Psp6I CCWGG 4 cut(s) 147, 402, 562, 803
PspCI CACGTG 1 cut(s) 744
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 4 cut(s) 147, 402, 562, 803
PspN4I GGNNCC 4 cut(s) 287, 298, 453, 552
PspOMI GGGCCC 1 cut(s) 550
PspPI GGNCC 5 cut(s) 151, 285, 493, 550, 551
RsaI GTAC 3 cut(s) 281, 593, 631
RsaNI GTAC 3 cut(s) 280, 592, 630
RseI CAYNNNNRTG 1 cut(s) 705
SapI GCTCTTC 1 cut(s) 370
SatI GCNGC 7 cut(s) 164, 167, 179, 196, 274, 322, 649
Sau3AI GATC 3 cut(s) 23, 27, 475
Sau96I GGNCC 5 cut(s) 151, 285, 493, 550, 551
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 4 cut(s) 149, 404, 564, 805
SduI GDGCHC 5 cut(s) 380, 547, 554, 606, 663
SinI GGWCC 2 cut(s) 285, 493
SmiMI CAYNNNNRTG 1 cut(s) 705
Sse9I AATT 2 cut(s) 232, 436
StyD4I CCNGG 4 cut(s) 147, 402, 562, 803
TaaI ACNGT 1 cut(s) 120
TaiI ACGT 2 cut(s) 678, 746
TaqI TCGA 2 cut(s) 26, 122
TaqII GACCGA 1 cut(s) 188
TasI AATT 2 cut(s) 232, 436
TauI GCSGC 5 cut(s) 166, 181, 276, 324, 651
TfiI GAWTC 1 cut(s) 200
TscAI CASTG 2 cut(s) 191, 402
TseI GCWGC 2 cut(s) 166, 195
TspGWI ACGGA 2 cut(s) 406, 488
TspRI CASTG 2 cut(s) 191, 402
VpaK11BI GGWCC 2 cut(s) 285, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.