pycom08g03230

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012880
Physical Location & Seq
Forward (+)
2528384 .. 2529145
762 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03230.1

Sequence Viewer

Length: 762 bp
ATGCCGTTTGACTCCGCCGACGCGCAAATGCGACCGTTGACGACGATGAGAGTCCAGCACAGTCTGGGTCACCCACCGCCGCAGCAGACAGAGCCACTTCACTGCCCCCGTTGCGATTCCGACAACACCAAGTTCTGCTACTACAACAATTACAACCTCGCCCAGCCACGCCACTTCTGCAAGTCCTGCCGCCGCTACTGGACCCACGGTGGCACCCTCCGAAATGTCCCCGTCGGCGGGGGCTCCCGCAAGAACACCAAGCGTTCTCGCCCGTCCTGCTCTTCCTCCTCGGGCTCGCCCTCCTCGTCTTCACTGACACAGGAGGTCAGCTCCGTCGCGCCCACAAACGCCATTTCCGTGCCGGTGGAACCGAAGCCGGAGACGGCGTTGGATCACGAAAAACTGATGGACTTGAACGAGAGCGCTGTCGGGAACAGCGGCTTCACTTCTCTGTTGAGTGCTCAGGGCCCACCCGCACCTGGGTTCTTGGCGCTTGGCGGGTTTGGGTATGGATTCGGGTACGGGTTGGGACTCGGGCAAGGGTTAGACGAAGTTGGTACTTTTGGCTACGGCGGCGGCAGAGGCGTGCTCGCTTTTCCCGAGGAAGGTGACTTTGCAAATATTAACCACAACAACAATGGAGCTGTTTCTGGGGGTTCTGTCGGGTGCAACACGTGGCAGATTATGACTGGTGGTGATGGAAGGATTGCTGAGGACAACGGGGATTGCTTTACCTGGCCGGAGCTTGAGATTTCGAAGTAA

Protein Analysis

254

Amino Acids

26.85

Weight (kDa)

5.96

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 32 - 87 7.1e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47655
fragaria_vesca FvH4_2g40660
malus_domestica MD08G1040100.v1.1 MD15G1034500.v1.1
prunus_persica Prupe.1G388600_v2.0.a1 Prupe.1G388600_v2.0.a1
pyrus_communis pycom08g03230 pycom15g03180
rosa_chinensis RchiOBHm_Chr6g0300001
rosa_laevigata RLG00000011373
rosa_multiflora Rmu_sc0001370.1_g000002 Rmu_sc0011230.1_g000001
rosa_roxburghii Rroxscaffold_7G00168210
rosa_rugosa Rorug06G0293400
rosa_samantha Rh6AG405100 Rh6BG413400 Rh6CG420500 Rh6DG406100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AccII CGCG 2 cut(s) 23, 338
AclWI GGATC 1 cut(s) 399
AcoI YGGCCR 1 cut(s) 737
AcvI CACGTG 1 cut(s) 675
AfaI GTAC 2 cut(s) 521, 559
AfeI AGCGCT 1 cut(s) 424
AfiI CCNNNNNNNGG 5 cut(s) 236, 237, 479, 480, 605
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 1 cut(s) 415
AjnI CCWGG 2 cut(s) 478, 734
AluBI AGCT 3 cut(s) 330, 644, 745
AluI AGCT 3 cut(s) 330, 644, 745
Alw21I GWGCWC 2 cut(s) 463, 591
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 1 cut(s) 399
Ama87I CYCGRG 3 cut(s) 289, 533, 599
Aor51HI AGCGCT 1 cut(s) 424
AoxI GGCC 2 cut(s) 466, 737
ApaI GGGCCC 1 cut(s) 470
ApeKI GCWGC 1 cut(s) 82
AspLEI GCGC 4 cut(s) 25, 340, 425, 493
AspS9I GGNCC 3 cut(s) 201, 466, 467
AsuHPI GGTGA 3 cut(s) 62, 620, 707
AsuII TTCGAA 1 cut(s) 755
AvaI CYCGRG 3 cut(s) 289, 533, 599
AvaII GGWCC 1 cut(s) 201
BaeGI GKGCMC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 3 cut(s) 245, 296, 470
BbrPI CACGTG 1 cut(s) 675
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 2 cut(s) 463, 591
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 1 cut(s) 94
BccI CCATC 2 cut(s) 400, 692
BceAI ACGGC 2 cut(s) 399, 586
BciT130I CCWGG 2 cut(s) 480, 736
BcoDI GTCTC 1 cut(s) 374
BfoI RGCGCY 2 cut(s) 426, 494
BisI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
BlsI GCNGC 7 cut(s) 81, 84, 191, 194, 440, 575, 578
Bme1390I CCNGG 2 cut(s) 480, 736
Bme18I GGWCC 1 cut(s) 201
BmeT110I CYCGRG 3 cut(s) 289, 533, 599
BmgT120I GGNCC 3 cut(s) 201, 466, 467
BmiI GGNNCC 5 cut(s) 203, 214, 244, 369, 468
BmrFI CCNGG 2 cut(s) 480, 736
BpiI GAAGAC 1 cut(s) 300
BplI GAGNNNNNCTC 4 cut(s) 314, 346, 573, 605
Bpu10I CCTNAGC 2 cut(s) 462, 711
Bpu14I TTCGAA 1 cut(s) 755
BsaAI YACGTR 1 cut(s) 675
BsaJI CCNNGG 4 cut(s) 205, 288, 479, 600
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 237, 479, 480, 605
Bse118I RCCGGY 1 cut(s) 361
Bse1I ACTGG 2 cut(s) 203, 694
BseBI CCWGG 2 cut(s) 480, 736
BseDI CCNNGG 4 cut(s) 205, 288, 479, 600
BseLI CCNNNNNNNGG 5 cut(s) 236, 237, 479, 480, 605
BseMII CTCAG 2 cut(s) 476, 702
BseNI ACTGG 2 cut(s) 203, 694
BseRI GAGGAG 2 cut(s) 277, 292
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 1 cut(s) 94
BseYI CCCAGC 1 cut(s) 162
Bsh1236I CGCG 2 cut(s) 23, 338
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 468, 739
BshNI GGYRCC 1 cut(s) 212
BsiEI CGRYCG 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 463, 591
BsiHKCI CYCGRG 3 cut(s) 289, 533, 599
BsiSI CCGG 3 cut(s) 362, 377, 740
BslFI GGGAC 2 cut(s) 212, 543
BslI CCNNNNNNNGG 5 cut(s) 236, 237, 479, 480, 605
BsmAI GTCTC 1 cut(s) 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 212, 543
BsnI GGCC 2 cut(s) 468, 739
BsoBI CYCGRG 3 cut(s) 289, 533, 599
Bsp119I TTCGAA 1 cut(s) 755
Bsp120I GGGCCC 1 cut(s) 466
Bsp1286I GDGCHC 5 cut(s) 245, 296, 463, 470, 591
Bsp143I GATC 1 cut(s) 391
BspANI GGCC 2 cut(s) 468, 739
BspCNI CTCAG 2 cut(s) 475, 703
BspFNI CGCG 2 cut(s) 23, 338
BspLI GGNNCC 5 cut(s) 203, 214, 244, 369, 468
BspPI GGATC 1 cut(s) 399
BspQI GCTCTTC 1 cut(s) 286
BspT104I TTCGAA 1 cut(s) 755
BspT107I GGYRCC 1 cut(s) 212
BsrFI RCCGGY 1 cut(s) 361
BsrI ACTGG 2 cut(s) 203, 694
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 4 cut(s) 205, 288, 479, 600
BssMI GATC 1 cut(s) 391
Bst2UI CCWGG 2 cut(s) 480, 736
Bst4CI ACNGT 3 cut(s) 36, 62, 209
Bst6I CTCTTC 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 675
BstBI TTCGAA 1 cut(s) 755
BstC8I GCNNGC 3 cut(s) 296, 587, 591
BstDEI CTNAG 2 cut(s) 462, 711
BstDSI CCRYGG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 68
BstFNI CGCG 2 cut(s) 23, 338
BstH2I RGCGCY 2 cut(s) 426, 494
BstHHI GCGC 4 cut(s) 25, 340, 425, 493
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 1 cut(s) 391
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 276, 573, 582
BstNI CCWGG 2 cut(s) 480, 736
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 478, 734
BstSLI GKGCMC 1 cut(s) 470
BstUI CGCG 2 cut(s) 23, 338
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 300
BsuRI GGCC 2 cut(s) 468, 739
BtgI CCRYGG 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 100, 311
Cac8I GCNNGC 3 cut(s) 296, 587, 591
CfoI GCGC 4 cut(s) 25, 340, 425, 493
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 3 cut(s) 201, 466, 467
CseI GACGC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 520, 558
CviQI GTAC 2 cut(s) 520, 558
DdeI CTNAG 2 cut(s) 462, 711
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 737
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
EciI GGCGGA 1 cut(s) 4
Eco24I GRGCYC 3 cut(s) 245, 296, 470
Eco47I GGWCC 1 cut(s) 201
Eco47III AGCGCT 1 cut(s) 424
Eco72I CACGTG 1 cut(s) 675
Eco88I CYCGRG 3 cut(s) 289, 533, 599
Eco91I GGTNACC 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 466
EcoO65I GGTNACC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 478, 734
EcoT38I GRGCYC 3 cut(s) 245, 296, 470
Esp3I CGTCTC 1 cut(s) 374
FaiI YATR 2 cut(s) 510, 686
FaqI GGGAC 2 cut(s) 212, 543
FauI CCCGC 4 cut(s) 230, 254, 481, 491
Fnu4HI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
FriOI GRGCYC 3 cut(s) 245, 296, 470
Fsp4HI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
GlaI GCGC 4 cut(s) 24, 339, 424, 492
GluI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
GsaI CCCAGC 1 cut(s) 166
HaeII RGCGCY 2 cut(s) 426, 494
HaeIII GGCC 2 cut(s) 468, 739
HapII CCGG 3 cut(s) 362, 377, 740
HgaI GACGC 1 cut(s) 29
HhaI GCGC 4 cut(s) 25, 340, 425, 493
Hin6I GCGC 4 cut(s) 23, 338, 423, 491
HinP1I GCGC 4 cut(s) 23, 338, 423, 491
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HinfI GANTC 5 cut(s) 11, 51, 116, 513, 531
HpaII CCGG 3 cut(s) 362, 377, 740
HphI GGTGA 3 cut(s) 62, 620, 707
Hpy166II GTNNAC 1 cut(s) 39
Hpy188I TCNGA 2 cut(s) 121, 221
Hpy188III TCNNGA 3 cut(s) 395, 430, 599
Hpy8I GTNNAC 1 cut(s) 39
Hpy99I CGWCG 4 cut(s) 23, 46, 236, 338
HpyAV CCTTC 2 cut(s) 599, 696
HpyCH4III ACNGT 3 cut(s) 36, 62, 209
HpyCH4IV ACGT 1 cut(s) 674
HpyCH4V TGCA 3 cut(s) 180, 617, 669
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 276, 573, 582
HpyF3I CTNAG 2 cut(s) 462, 711
HpySE526I ACGT 1 cut(s) 674
HspAI GCGC 4 cut(s) 23, 338, 423, 491
Kzo9I GATC 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 4 cut(s) 248, 335, 641, 742
Lsp1109I GCAGC 1 cut(s) 94
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 2 cut(s) 68, 608
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 2 cut(s) 273, 300
MhlI GDGCHC 5 cut(s) 245, 296, 463, 470, 591
MluCI AATT 1 cut(s) 148
MlyI GAGTC 3 cut(s) 5, 60, 525
MmeI TCCRAC 2 cut(s) 144, 369
MseI TTAA 1 cut(s) 624
MslI CAYNNNNRTG 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 438
MspI CCGG 3 cut(s) 362, 377, 740
MspR9I CCNGG 2 cut(s) 480, 736
MvaI CCWGG 2 cut(s) 480, 736
MvnI CGCG 2 cut(s) 23, 338
MwoI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 276, 573, 582
NdeII GATC 1 cut(s) 391
NlaIV GGNNCC 5 cut(s) 203, 214, 244, 369, 468
NmuCI GTSAC 2 cut(s) 68, 608
NspV TTCGAA 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 3 cut(s) 302, 354, 597
PfeI GAWTC 2 cut(s) 116, 513
PkrI GCNGC 7 cut(s) 81, 84, 191, 194, 440, 575, 578
PleI GAGTC 3 cut(s) 5, 59, 525
PmaCI CACGTG 1 cut(s) 675
PmlI CACGTG 1 cut(s) 675
PpsI GAGTC 3 cut(s) 5, 59, 525
Ppu21I YACGTR 1 cut(s) 675
Psp6I CCWGG 2 cut(s) 478, 734
PspCI CACGTG 1 cut(s) 675
PspEI GGTNACC 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 2 cut(s) 478, 734
PspN4I GGNNCC 5 cut(s) 203, 214, 244, 369, 468
PspOMI GGGCCC 1 cut(s) 466
PspPI GGNCC 3 cut(s) 201, 466, 467
RsaI GTAC 2 cut(s) 521, 559
RsaNI GTAC 2 cut(s) 520, 558
RseI CAYNNNNRTG 1 cut(s) 356
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 7 cut(s) 80, 83, 190, 193, 439, 574, 577
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 3 cut(s) 201, 466, 467
SchI GAGTC 3 cut(s) 5, 60, 525
ScrFI CCNGG 2 cut(s) 480, 736
SduI GDGCHC 5 cut(s) 245, 296, 463, 470, 591
SetI ASST 9 cut(s) 159, 327, 332, 481, 610, 646, 677, 737, 747
SfuI TTCGAA 1 cut(s) 755
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
Sse9I AATT 1 cut(s) 148
SspI AATATT 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 478, 734
TaaI ACNGT 3 cut(s) 36, 62, 209
TaiI ACGT 1 cut(s) 677
TaqI TCGA 1 cut(s) 755
TasI AATT 1 cut(s) 148
TauI GCSGC 6 cut(s) 82, 192, 195, 441, 576, 579
TfiI GAWTC 2 cut(s) 116, 513
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 2 cut(s) 107, 318
TseFI GTSAC 2 cut(s) 68, 608
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 2 cut(s) 68, 608
TspGWI ACGGA 2 cut(s) 322, 346
TspRI CASTG 2 cut(s) 107, 318
VpaK11BI GGWCC 1 cut(s) 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.