MD15G1034500.v1.1

Dof domain, zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012880
Physical Location & Seq
Forward (+)
2439144 .. 2439890
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1034500.v1.1.491

Sequence Viewer

Length: 747 bp
ATGCCGTTTGACTCTGCCGACGCGCTAATGCAACCGTCGATGACGACGAGAGTCCAGCACAGCCTGGGCCACCCTCCGCCGCAGCAGACCGAGCCGCTTCACTGCCCCCGCTGCGATTCTGACAACACCAAGTTCTGCTACTACAACAATTACAACCTCGCCCAGCCACGCCACTTCTGCAAGTCCTGCCGCCGATACTGGACCCACGGCGGAACCCTCCGCAATGTCCCCGTCGGCGGCGGGTCTCGCAAGAACACCAAGCGTTCTCGCCCATCCTGCTCTTCCTCCTCGGGCTCGCCCTCCTCCTCTTCACTGACCCAGGAGGTCAGCTCCGTCGCGCCCGATAAACCCAATTCCTTTCCGGTGGAACCGAAGCCTGAGACGGCGACGGATCACGAAAAACTGATGGACCTGAACGAGAGCGCTGTTGGGAACGGTGGCTTCACTTCTCTGTTGAGTGCTCAGGGCCCACCTGCATCTGGCTTCATGGCGTTTAGCGGGTTCGGGTACGGGTTGGGGCTCGGGCAAGGGTTTGACGAAGTGGGTACTTTCGGCTACGACGGCGGCAGAGGAGTGCTCGCTTATCCCGATGTCGGTGATTTTACAAATGTTATCCACAACAATGGAGCTGTTTCTGGGGGTTCTGTCGGGTGCAACACGTGGCAGATTATGACTGGTGGTGATGGTGGGATAGCAGAGAACAATGGTGATTGCTTTGCCTGGCCGGAGCTGGAGACTTCCAAGTAA

Protein Analysis

249

Amino Acids

26.31

Weight (kDa)

5.77

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 32 - 87 6.9e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47655
fragaria_vesca FvH4_2g40660
malus_domestica MD08G1040100.v1.1 MD15G1034500.v1.1
prunus_persica Prupe.1G388600_v2.0.a1 Prupe.1G388600_v2.0.a1
pyrus_communis pycom08g03230 pycom15g03180
rosa_chinensis RchiOBHm_Chr6g0300001
rosa_laevigata RLG00000011373
rosa_multiflora Rmu_sc0001370.1_g000002 Rmu_sc0011230.1_g000001
rosa_roxburghii Rroxscaffold_7G00168210
rosa_rugosa Rorug06G0293400
rosa_samantha Rh6AG405100 Rh6BG413400 Rh6CG420500 Rh6DG406100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 481
Acc36I ACCTGC 1 cut(s) 481
AccII CGCG 2 cut(s) 23, 338
AclWI GGATC 1 cut(s) 399
AcoI YGGCCR 1 cut(s) 722
AcvI CACGTG 1 cut(s) 660
AfaI GTAC 2 cut(s) 509, 547
AfeI AGCGCT 1 cut(s) 424
AfiI CCNNNNNNNGG 3 cut(s) 236, 479, 593
AflIII ACRYGT 1 cut(s) 657
AjnI CCWGG 3 cut(s) 63, 318, 719
AluBI AGCT 3 cut(s) 330, 629, 730
AluI AGCT 3 cut(s) 330, 629, 730
Alw21I GWGCWC 2 cut(s) 463, 579
Alw26I GTCTC 3 cut(s) 249, 374, 728
AlwI GGATC 1 cut(s) 399
Ama87I CYCGRG 2 cut(s) 289, 521
Aor51HI AGCGCT 1 cut(s) 424
AoxI GGCC 3 cut(s) 67, 466, 722
ApaI GGGCCC 1 cut(s) 470
ApeKI GCWGC 2 cut(s) 82, 111
AspLEI GCGC 3 cut(s) 25, 340, 425
AspS9I GGNCC 5 cut(s) 67, 201, 409, 466, 467
AsuHPI GGTGA 3 cut(s) 608, 692, 719
AvaI CYCGRG 2 cut(s) 289, 521
AvaII GGWCC 2 cut(s) 201, 409
BaeGI GKGCMC 1 cut(s) 470
BanII GRGCYC 3 cut(s) 296, 470, 522
BbrPI CACGTG 1 cut(s) 660
Bbv12I GWGCWC 2 cut(s) 463, 579
BbvI GCAGC 2 cut(s) 94, 98
BccI CCATC 3 cut(s) 280, 400, 677
BceAI ACGGC 3 cut(s) 223, 399, 577
BciT130I CCWGG 3 cut(s) 65, 320, 721
BcoDI GTCTC 3 cut(s) 249, 374, 728
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 481
BisI GCNGC 7 cut(s) 80, 83, 95, 112, 190, 238, 565
BlsI GCNGC 7 cut(s) 81, 84, 96, 113, 191, 239, 566
Bme1390I CCNGG 3 cut(s) 65, 320, 721
Bme18I GGWCC 2 cut(s) 201, 409
BmeT110I CYCGRG 2 cut(s) 289, 521
BmgT120I GGNCC 5 cut(s) 67, 201, 409, 466, 467
BmiI GGNNCC 4 cut(s) 203, 214, 369, 468
BmrFI CCNGG 3 cut(s) 65, 320, 721
BmsI GCATC 1 cut(s) 485
BoxI GACNNNNGTC 1 cut(s) 50
BplI GAGNNNNNCTC 4 cut(s) 314, 346, 561, 593
Bpu10I CCTNAGC 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 660
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 4 cut(s) 64, 205, 288, 318
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 479, 593
Bse1I ACTGG 2 cut(s) 203, 679
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 3 cut(s) 65, 320, 721
BseDI CCNNGG 4 cut(s) 64, 205, 288, 318
BseGI GGATG 1 cut(s) 272
BseLI CCNNNNNNNGG 3 cut(s) 236, 479, 593
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 2 cut(s) 369, 476
BseNI ACTGG 2 cut(s) 203, 679
BseRI GAGGAG 4 cut(s) 277, 292, 295, 585
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 2 cut(s) 94, 98
BseYI CCCAGC 1 cut(s) 162
Bsh1236I CGCG 2 cut(s) 23, 338
BshFI GGCC 3 cut(s) 69, 468, 724
BsiHKAI GWGCWC 2 cut(s) 463, 579
BsiHKCI CYCGRG 2 cut(s) 289, 521
BsiSI CCGG 2 cut(s) 362, 725
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 236, 479, 593
BsmAI GTCTC 3 cut(s) 249, 374, 728
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 3 cut(s) 69, 468, 724
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 2 cut(s) 289, 521
Bsp120I GGGCCC 1 cut(s) 466
Bsp1286I GDGCHC 5 cut(s) 296, 463, 470, 522, 579
Bsp143I GATC 1 cut(s) 391
BspANI GGCC 3 cut(s) 69, 468, 724
BspCNI CTCAG 2 cut(s) 370, 475
BspFNI CGCG 2 cut(s) 23, 338
BspLI GGNNCC 4 cut(s) 203, 214, 369, 468
BspMI ACCTGC 1 cut(s) 481
BspPI GGATC 1 cut(s) 399
BspQI GCTCTTC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 229
BsrI ACTGG 2 cut(s) 203, 679
BssECI CCNNGG 4 cut(s) 64, 205, 288, 318
BssMI GATC 1 cut(s) 391
Bst2UI CCWGG 3 cut(s) 65, 320, 721
Bst4CI ACNGT 2 cut(s) 36, 437
Bst6I CTCTTC 2 cut(s) 286, 313
BstAPI GCANNNNNTGC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 660
BstC8I GCNNGC 2 cut(s) 296, 579
BstDEI CTNAG 2 cut(s) 378, 462
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 1 cut(s) 272
BstFNI CGCG 2 cut(s) 23, 338
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 3 cut(s) 25, 340, 425
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 3 cut(s) 249, 374, 728
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 246, 276, 561
BstNI CCWGG 3 cut(s) 65, 320, 721
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 3 cut(s) 63, 318, 719
BstSLI GKGCMC 1 cut(s) 470
BstUI CGCG 2 cut(s) 23, 338
BstV1I GCAGC 2 cut(s) 94, 98
BstXI CCANNNNNNTGG 1 cut(s) 623
BsuRI GGCC 3 cut(s) 69, 468, 724
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 272
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 100, 311
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 296, 579
CfoI GCGC 3 cut(s) 25, 340, 425
Cfr13I GGNCC 5 cut(s) 67, 201, 409, 466, 467
CseI GACGC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 508, 546
CviAII CATG 1 cut(s) 487
CviQI GTAC 2 cut(s) 508, 546
DdeI CTNAG 2 cut(s) 378, 462
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 722
Eam1104I CTCTTC 2 cut(s) 286, 313
EarI CTCTTC 2 cut(s) 286, 313
EciI GGCGGA 2 cut(s) 66, 225
Eco24I GRGCYC 3 cut(s) 296, 470, 522
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 201, 409
Eco47III AGCGCT 1 cut(s) 424
Eco72I CACGTG 1 cut(s) 660
Eco88I CYCGRG 2 cut(s) 289, 521
EcoO109I RGGNCCY 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 63, 318, 719
EcoT38I GRGCYC 3 cut(s) 296, 470, 522
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 1 cut(s) 490
FaiI YATR 2 cut(s) 488, 671
FaqI GGGAC 1 cut(s) 212
FatI CATG 1 cut(s) 486
FauI CCCGC 3 cut(s) 116, 233, 491
Fnu4HI GCNGC 7 cut(s) 80, 83, 95, 112, 190, 238, 565
FokI GGATG 1 cut(s) 259
FriOI GRGCYC 3 cut(s) 296, 470, 522
Fsp4HI GCNGC 7 cut(s) 80, 83, 95, 112, 190, 238, 565
GlaI GCGC 3 cut(s) 24, 339, 424
GluI GCNGC 7 cut(s) 80, 83, 95, 112, 190, 238, 565
GsaI CCCAGC 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 3 cut(s) 69, 468, 724
HapII CCGG 2 cut(s) 362, 725
HgaI GACGC 1 cut(s) 29
HhaI GCGC 3 cut(s) 25, 340, 425
Hin1II CATG 1 cut(s) 490
Hin6I GCGC 3 cut(s) 23, 338, 423
HinP1I GCGC 3 cut(s) 23, 338, 423
HinfI GANTC 3 cut(s) 11, 51, 116
HpaII CCGG 2 cut(s) 362, 725
HphI GGTGA 3 cut(s) 608, 692, 719
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 2 cut(s) 395, 587
Hpy99I CGWCG 7 cut(s) 23, 40, 49, 236, 338, 391, 563
HpyCH4III ACNGT 2 cut(s) 36, 437
HpyCH4IV ACGT 1 cut(s) 659
HpyCH4V TGCA 4 cut(s) 31, 180, 476, 654
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 246, 276, 561
HpyF3I CTNAG 2 cut(s) 378, 462
HpySE526I ACGT 1 cut(s) 659
Hsp92II CATG 1 cut(s) 490
HspAI GCGC 3 cut(s) 23, 338, 423
Kzo9I GATC 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 3 cut(s) 335, 626, 727
Lsp1109I GCAGC 2 cut(s) 94, 98
LweI GCATC 1 cut(s) 485
MaeII ACGT 1 cut(s) 659
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 2 cut(s) 273, 300
MhlI GDGCHC 5 cut(s) 296, 463, 470, 522, 579
MluCI AATT 2 cut(s) 148, 352
MlyI GAGTC 2 cut(s) 5, 60
MslI CAYNNNNRTG 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 2 cut(s) 362, 725
MspR9I CCNGG 3 cut(s) 65, 320, 721
MvaI CCWGG 3 cut(s) 65, 320, 721
MvnI CGCG 2 cut(s) 23, 338
MwoI GCNNNNNNNGC 7 cut(s) 91, 111, 177, 186, 246, 276, 561
NdeII GATC 1 cut(s) 391
NlaIII CATG 1 cut(s) 490
NlaIV GGNNCC 4 cut(s) 203, 214, 369, 468
PaqCI CACCTGC 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 585
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 7 cut(s) 81, 84, 96, 113, 191, 239, 566
PleI GAGTC 2 cut(s) 5, 59
PmaCI CACGTG 1 cut(s) 660
PmlI CACGTG 1 cut(s) 660
PpsI GAGTC 2 cut(s) 5, 59
Ppu21I YACGTR 1 cut(s) 660
PshAI GACNNNNGTC 1 cut(s) 50
Psp6I CCWGG 3 cut(s) 63, 318, 719
PspCI CACGTG 1 cut(s) 660
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 3 cut(s) 63, 318, 719
PspN4I GGNNCC 4 cut(s) 203, 214, 369, 468
PspOMI GGGCCC 1 cut(s) 466
PspPI GGNCC 5 cut(s) 67, 201, 409, 466, 467
RsaI GTAC 2 cut(s) 509, 547
RsaNI GTAC 2 cut(s) 508, 546
RseI CAYNNNNRTG 1 cut(s) 621
SapI GCTCTTC 1 cut(s) 286
SatI GCNGC 7 cut(s) 80, 83, 95, 112, 190, 238, 565
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 5 cut(s) 67, 201, 409, 466, 467
SchI GAGTC 2 cut(s) 5, 60
ScrFI CCNGG 3 cut(s) 65, 320, 721
SduI GDGCHC 5 cut(s) 296, 463, 470, 522, 579
SetI ASST 8 cut(s) 159, 327, 332, 414, 475, 631, 662, 732
SfaNI GCATC 1 cut(s) 485
SinI GGWCC 2 cut(s) 201, 409
SmiMI CAYNNNNRTG 1 cut(s) 621
Sse9I AATT 2 cut(s) 148, 352
StyD4I CCNGG 3 cut(s) 63, 318, 719
TaaI ACNGT 2 cut(s) 36, 437
TaiI ACGT 1 cut(s) 662
TaqI TCGA 1 cut(s) 38
TaqII GACCGA 1 cut(s) 104
TasI AATT 2 cut(s) 148, 352
TauI GCSGC 5 cut(s) 82, 97, 192, 240, 567
TfiI GAWTC 1 cut(s) 116
TscAI CASTG 2 cut(s) 107, 318
TseI GCWGC 2 cut(s) 82, 111
TspDTI ATGAA 1 cut(s) 475
TspGWI ACGGA 2 cut(s) 322, 404
TspRI CASTG 2 cut(s) 107, 318
VpaK11BI GGWCC 2 cut(s) 201, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.