RchiOBHm_Chr6g0300001

dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012880
Physical Location & Seq
Reverse (-)
60991035 .. 60992532
1498 bp
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UTR
Exon/CDS
Intron
PRQ26939

Sequence Viewer

Length: 840 bp
ATGCCCGCTAGTACTACGCCTCCTGAAGTCTCCGGGATCAAACGGCCGAGACTGATCCAGAGCATGCCTGCTGCTTACCCACCGCCATCCTCTGCCGACCCGCTTCCCTGCCCGCGCTGCAACTCCACCCAGACGAAGTTCTGCTACTACAACAACTACAACCTCGCTCAGCCCCGCCACTTCTGCAAGGGCTGCCGCCGCTACTGGACTCGTGGTGGGGCCCTCCGCAACGTCCCCGTCGGCGGCGGAACTCGCAACAAGAAACCCAGACTCCCTCACCGCGCCTCCTCCTCCAACTCCTCCGGCTCGCCTTCCTCTTCGTCCCTCACCCACGAGACCACCGCACCGAACAAACCCACTTCCGACTCCGCTGTGGAAAAGCCCGATTTGTCCTCCGACCAACAACAACAACAACTTCTCAACCTGAACGACAACGTTACGAGTGAAGGAGGGTTTTCGTTTCTGACGATGATGAACGCTCAGGAACAACCTGGGTTTTTGTCCCTTGGTGGGTATGGGTACGGGTCGGGGTTTGCCCATGGGTTCCAGCAAGGTTACGGGTACGGCGCCAGAGCCGCAGGGGTTGATTTTCCGACGGAGATGGTCGGTGCTTTTGCTGGTGTCTATGGTGTTCCTGGGGCTGCTGCTTCTTCCGGGTTTGACGTCAACACGTGGCAGATGGCGGTTGCTGATCATGAAGGTCAAGGTGGTGGATTGGTTGATTGGCCGGAGGTTCAGATTCTCGATTCCGTGCCAGCTGTCAGTGGTGGTGGTGTCAAGGAAGAAAAGGTGGCAGCTGATACCCCAACTGACCCTTGTCTTAATTTCCCTGCGTTCTGA

Protein Analysis

279

Amino Acids

29.46

Weight (kDa)

6.51

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 34 - 88 1e-31 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47655
fragaria_vesca FvH4_2g40660
malus_domestica MD08G1040100.v1.1 MD15G1034500.v1.1
prunus_persica Prupe.1G388600_v2.0.a1 Prupe.1G388600_v2.0.a1
pyrus_communis pycom08g03230 pycom15g03180
rosa_chinensis RchiOBHm_Chr6g0300001
rosa_laevigata RLG00000011373
rosa_multiflora Rmu_sc0001370.1_g000002 Rmu_sc0011230.1_g000001
rosa_roxburghii Rroxscaffold_7G00168210
rosa_rugosa Rorug06G0293400
rosa_samantha Rh6AG405100 Rh6BG413400 Rh6CG420500 Rh6DG406100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 566
AccII CGCG 2 cut(s) 115, 282
AclI AACGTT 1 cut(s) 435
AclWI GGATC 2 cut(s) 44, 49
AcoI YGGCCR 2 cut(s) 44, 725
AcuI CTGAAG 1 cut(s) 45
AcvI CACGTG 1 cut(s) 672
AcyI GRCGYC 2 cut(s) 567, 663
AfaI GTAC 3 cut(s) 13, 521, 563
AfiI CCNNNNNNNGG 2 cut(s) 242, 510
AflIII ACRYGT 1 cut(s) 669
AjnI CCWGG 2 cut(s) 490, 634
AluBI AGCT 2 cut(s) 758, 797
AluI AGCT 2 cut(s) 758, 797
Alw26I GTCTC 3 cut(s) 34, 43, 329
AlwI GGATC 2 cut(s) 44, 49
AoxI GGCC 3 cut(s) 44, 219, 725
ApaI GGGCCC 1 cut(s) 223
ApeKI GCWGC 6 cut(s) 71, 117, 192, 641, 644, 794
AspLEI GCGC 3 cut(s) 117, 284, 569
AspS9I GGNCC 2 cut(s) 219, 220
AsuC2I CCSGG 2 cut(s) 34, 655
AsuHPI GGTGA 2 cut(s) 269, 319
BaeGI GKGCMC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 1 cut(s) 223
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BauI CACGAG 2 cut(s) 210, 332
BbrPI CACGTG 1 cut(s) 672
BbvI GCAGC 6 cut(s) 58, 104, 179, 628, 631, 806
BccI CCATC 3 cut(s) 94, 595, 673
BceAI ACGGC 2 cut(s) 59, 580
BciT130I CCWGG 2 cut(s) 492, 636
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 34, 655
BcoDI GTCTC 3 cut(s) 34, 43, 329
BfaI CTAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 570
BlpI GCTNAGC 1 cut(s) 168
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 4 cut(s) 34, 492, 636, 655
BmgT120I GGNCC 2 cut(s) 219, 220
BmiI GGNNCC 4 cut(s) 220, 221, 545, 568
BmrFI CCNGG 4 cut(s) 34, 492, 636, 655
BoxI GACNNNNGTC 1 cut(s) 816
Bpu10I CCTNAGC 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 168
BpuMI CCSGG 2 cut(s) 34, 655
BsaAI YACGTR 1 cut(s) 672
BsaHI GRCGYC 2 cut(s) 567, 663
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 4 cut(s) 491, 505, 538, 635
BsaXI ACNNNNNCTCC 5 cut(s) 34, 255, 269, 285, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 510
Bse1I ACTGG 1 cut(s) 209
BseBI CCWGG 2 cut(s) 492, 636
BseDI CCNNGG 4 cut(s) 491, 505, 538, 635
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 2 cut(s) 242, 510
BseMII CTCAG 2 cut(s) 182, 494
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 3 cut(s) 277, 280, 289
BseSI GKGCMC 1 cut(s) 223
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 6 cut(s) 58, 104, 179, 628, 631, 806
Bsh1236I CGCG 2 cut(s) 115, 282
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 3 cut(s) 46, 221, 727
BshNI GGYRCC 1 cut(s) 566
BsiEI CGRYCG 1 cut(s) 47
BsiSI CCGG 4 cut(s) 33, 303, 654, 728
BslFI GGGAC 3 cut(s) 218, 307, 487
BslI CCNNNNNNNGG 2 cut(s) 242, 510
BsmAI GTCTC 3 cut(s) 34, 43, 329
BsmFI GGGAC 3 cut(s) 218, 307, 487
BsnI GGCC 3 cut(s) 46, 221, 727
Bso31I GGTCTC 1 cut(s) 329
Bsp120I GGGCCC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 3 cut(s) 36, 54, 691
Bsp1720I GCTNAGC 1 cut(s) 168
Bsp19I CCATGG 1 cut(s) 538
BspANI GGCC 3 cut(s) 46, 221, 727
BspCNI CTCAG 2 cut(s) 181, 493
BspFNI CGCG 2 cut(s) 115, 282
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 4 cut(s) 220, 221, 545, 568
BspPI GGATC 2 cut(s) 44, 49
BspT107I GGYRCC 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 329
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 4 cut(s) 491, 505, 538, 635
BssMI GATC 3 cut(s) 36, 54, 691
BssNI GRCGYC 2 cut(s) 567, 663
BssSI CACGAG 2 cut(s) 210, 332
BssT1I CCWWGG 2 cut(s) 505, 538
Bst2BI CACGAG 2 cut(s) 210, 332
Bst2UI CCWGG 2 cut(s) 492, 636
Bst6I CTCTTC 1 cut(s) 322
BstACI GRCGYC 2 cut(s) 567, 663
BstAPI GCANNNNNTGC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 672
BstC8I GCNNGC 6 cut(s) 6, 65, 69, 113, 308, 756
BstDEI CTNAG 2 cut(s) 168, 480
BstDSI CCRYGG 1 cut(s) 538
BstF5I GGATG 1 cut(s) 86
BstFNI CGCG 2 cut(s) 115, 282
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 3 cut(s) 117, 284, 569
BstKTI GATC 3 cut(s) 39, 57, 694
BstMAI GTCTC 3 cut(s) 34, 43, 329
BstMBI GATC 3 cut(s) 36, 54, 691
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 6 cut(s) 117, 183, 192, 198, 252, 575
BstNI CCWGG 2 cut(s) 492, 636
BstNSI RCATGY 1 cut(s) 67
BstPAI GACNNNNGTC 1 cut(s) 816
BstSCI CCNGG 4 cut(s) 32, 490, 634, 653
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 2 cut(s) 115, 282
BstV1I GCAGC 6 cut(s) 58, 104, 179, 628, 631, 806
BstZI CGGCCG 1 cut(s) 44
BsuRI GGCC 3 cut(s) 46, 221, 727
BtgI CCRYGG 1 cut(s) 538
BtsCI GGATG 1 cut(s) 86
BtsIMutI CAGTG 1 cut(s) 769
Cac8I GCNNGC 6 cut(s) 6, 65, 69, 113, 308, 756
CciI TCATGA 1 cut(s) 694
CfoI GCGC 3 cut(s) 117, 284, 569
Cfr13I GGNCC 2 cut(s) 219, 220
Csp6I GTAC 3 cut(s) 12, 520, 562
CviAII CATG 3 cut(s) 64, 539, 695
CviQI GTAC 3 cut(s) 12, 520, 562
DdeI CTNAG 2 cut(s) 168, 480
DinI GGCGCC 1 cut(s) 568
DpnI GATC 3 cut(s) 38, 56, 693
DpnII GATC 3 cut(s) 36, 54, 691
EaeI YGGCCR 2 cut(s) 44, 725
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
EciI GGCGGA 1 cut(s) 261
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 505, 538
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 329
Eco52I CGGCCG 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 45
Eco72I CACGTG 1 cut(s) 672
EcoO109I RGGNCCY 2 cut(s) 219, 220
EcoRII CCWGG 2 cut(s) 490, 634
EcoT14I CCWWGG 2 cut(s) 505, 538
EcoT38I GRGCYC 1 cut(s) 223
EgeI GGCGCC 1 cut(s) 568
EheI GGCGCC 1 cut(s) 568
ErhI CCWWGG 2 cut(s) 505, 538
FaeI CATG 3 cut(s) 67, 542, 698
FaiI YATR 5 cut(s) 65, 516, 540, 627, 696
FaqI GGGAC 3 cut(s) 218, 307, 487
FatI CATG 3 cut(s) 63, 538, 694
FauI CCCGC 4 cut(s) 13, 108, 120, 182
FbaI TGATCA 1 cut(s) 691
FokI GGATG 1 cut(s) 73
FriOI GRGCYC 1 cut(s) 223
FspBI CTAG 1 cut(s) 9
GlaI GCGC 3 cut(s) 116, 283, 568
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 3 cut(s) 46, 221, 727
HapII CCGG 4 cut(s) 33, 303, 654, 728
HhaI GCGC 3 cut(s) 117, 284, 569
Hin1I GRCGYC 2 cut(s) 567, 663
Hin1II CATG 3 cut(s) 67, 542, 698
Hin6I GCGC 3 cut(s) 115, 282, 567
HinP1I GCGC 3 cut(s) 115, 282, 567
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 5 cut(s) 208, 270, 365, 739, 746
HpaII CCGG 4 cut(s) 33, 303, 654, 728
HphI GGTGA 2 cut(s) 269, 319
Hpy166II GTNNAC 1 cut(s) 667
Hpy188I TCNGA 6 cut(s) 364, 397, 465, 594, 738, 839
Hpy188III TCNNGA 5 cut(s) 23, 58, 482, 695, 743
Hpy8I GTNNAC 1 cut(s) 667
Hpy99I CGWCG 2 cut(s) 242, 598
HpyAV CCTTC 3 cut(s) 321, 440, 692
HpyCH4IV ACGT 4 cut(s) 231, 435, 663, 671
HpyCH4V TGCA 2 cut(s) 120, 186
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 183, 192, 198, 252, 575
HpyF3I CTNAG 2 cut(s) 168, 480
HpySE526I ACGT 4 cut(s) 231, 435, 663, 671
Hsp92I GRCGYC 2 cut(s) 567, 663
Hsp92II CATG 3 cut(s) 67, 542, 698
HspAI GCGC 3 cut(s) 115, 282, 567
KasI GGCGCC 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 3 cut(s) 36, 54, 691
Lsp1109I GCAGC 6 cut(s) 58, 104, 179, 628, 631, 806
MaeI CTAG 1 cut(s) 9
MaeII ACGT 4 cut(s) 231, 435, 663, 671
MaeIII GTNAC 2 cut(s) 436, 554
MalI GATC 3 cut(s) 38, 56, 693
MboI GATC 3 cut(s) 36, 54, 691
MboII GAAGA 3 cut(s) 309, 642, 794
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 823
Mly113I GGCGCC 1 cut(s) 567
MlyI GAGTC 3 cut(s) 202, 264, 359
MmeI TCCRAC 4 cut(s) 318, 387, 420, 617
MseI TTAA 1 cut(s) 822
MspA1I CMGCKG 3 cut(s) 371, 758, 797
MspI CCGG 4 cut(s) 33, 303, 654, 728
MspR9I CCNGG 4 cut(s) 34, 492, 636, 655
MvaI CCWGG 2 cut(s) 492, 636
MvnI CGCG 2 cut(s) 115, 282
MwoI GCNNNNNNNGC 6 cut(s) 117, 183, 192, 198, 252, 575
NarI GGCGCC 1 cut(s) 567
NciI CCSGG 2 cut(s) 34, 655
NcoI CCATGG 1 cut(s) 538
NdeII GATC 3 cut(s) 36, 54, 691
NlaIII CATG 3 cut(s) 67, 542, 698
NlaIV GGNNCC 4 cut(s) 220, 221, 545, 568
NmeAIII GCCGAG 1 cut(s) 72
NspI RCATGY 1 cut(s) 67
PaeI GCATGC 1 cut(s) 67
PagI TCATGA 1 cut(s) 694
PcsI WCGNNNNNNNCGW 1 cut(s) 464
PfeI GAWTC 2 cut(s) 739, 746
PfoI TCCNGGA 1 cut(s) 32
PleI GAGTC 3 cut(s) 202, 264, 359
PluTI GGCGCC 1 cut(s) 570
PmaCI CACGTG 1 cut(s) 672
PmlI CACGTG 1 cut(s) 672
PpsI GAGTC 3 cut(s) 202, 264, 359
Ppu21I YACGTR 1 cut(s) 672
PshAI GACNNNNGTC 1 cut(s) 816
Psp1406I AACGTT 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 490, 634
PspCI CACGTG 1 cut(s) 672
PspGI CCWGG 2 cut(s) 490, 634
PspN4I GGNNCC 4 cut(s) 220, 221, 545, 568
PspOMI GGGCCC 1 cut(s) 219
PspPI GGNCC 2 cut(s) 219, 220
PvuII CAGCTG 2 cut(s) 758, 797
RsaI GTAC 3 cut(s) 13, 521, 563
RsaNI GTAC 3 cut(s) 12, 520, 562
SaqAI TTAA 1 cut(s) 822
Sau3AI GATC 3 cut(s) 36, 54, 691
Sau96I GGNCC 2 cut(s) 219, 220
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 3 cut(s) 202, 264, 359
ScrFI CCNGG 4 cut(s) 34, 492, 636, 655
SduI GDGCHC 1 cut(s) 223
SfoI GGCGCC 1 cut(s) 568
SphI GCATGC 1 cut(s) 67
Sse9I AATT 1 cut(s) 823
SspDI GGCGCC 1 cut(s) 566
SspMI CTAG 1 cut(s) 9
StyD4I CCNGG 4 cut(s) 32, 490, 634, 653
StyI CCWWGG 2 cut(s) 505, 538
TaiI ACGT 4 cut(s) 234, 438, 666, 674
TaqI TCGA 1 cut(s) 744
TasI AATT 1 cut(s) 823
TatI WGTACW 1 cut(s) 11
TauI GCSGC 4 cut(s) 198, 201, 246, 578
TfiI GAWTC 2 cut(s) 739, 746
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TscAI CASTG 1 cut(s) 769
TseI GCWGC 6 cut(s) 71, 117, 192, 641, 644, 794
TspDTI ATGAA 2 cut(s) 488, 711
TspGWI ACGGA 2 cut(s) 611, 739
TspRI CASTG 1 cut(s) 769
XceI RCATGY 1 cut(s) 67
XspI CTAG 1 cut(s) 9
ZraI GACGTC 1 cut(s) 664
ZrmI AGTACT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.