Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004438

Physical Location & Seq

Genomic Coordinates Reverse (-)

5549132 .. 5552962

Gene Structure 3831 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1070200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1053 bp

ATGGGTGAGGCTGTTGAGTTTTATCATGCAAACGGAAATGGGTTCTCAAAATTCAGTGAGAAGTTTGATCCTAAAGCAGTGGTACTACTAAACGGTAGCAACCACATAGAGAGAGATATAGATAGTTCCTGGTATGAAGAAACCATTGATGACGATCTGAAATGGTCATTTGCTCTCAATGTCAACGGTGTGCTGCATAAAGGAACTAGCGAGTACCAGGAGATAGCTTTATTGGACACAAAGCGTTTCGGGAAGGTACTGATAATTGATGGGAAGATGCAAAGTGCAGAAGTGGATGAATTTATATATCATGAATGCTTGATACATCCTGCACTCTTATGTCACCCAAAGCCTGAACGTATATTCATAATGGGAGGTGGGGAAGGGTCAGCTGCAAGGGAAGCACTCAAGCACAAATTACTGGACAAAGTTACCATGTGTGACATCGATCAGGAGGTGGTTGATTTCTGCCGCAGGCATTTGACAGTGAATCAGGAGGCATTTTCTCATCATAAGCTTAACCTTATTATAAATGATGCCAAGGCGGAATTGGAGAAGAGTTGTGAAAAATTTGATATCATAGTCGGGGATTTAGCAGACCCGGTTGAAGGAGGACCTTGCTATCAACTCTACACAAAATCCTTCTATGACAAAATTCTTAAACCTAAGCTTAATCATGGTGGCATTTTTGTGACCCAGGCTGGTCCAGCAGGCATTTTCACCCACAAAGAGGTCTTCACCTCTATATATAACACAATTAAACAGGTCTTCAAGTATGTGGTGCCATATGCAGCTCACATACCATCTTTTGCAGATACATGGGGATGGGTTATGGCCTCGGACGAGCCTTTTTTTATCAATGCTGAGAAAATGGACAGGAGGATAGAGGAAAGAATTGATGGGGAGCTAAACTACTTGAATGGTGCTCAGTTCATGTCCTCTGCAACCATGAACAAAACTGTTTCTTTATCGTTGCTGAATGAAACTCATGTCTACACTGAGGAAGATGCAAGGTTTGTCCATGGGCATGGGGTGGCTTACCGAATTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0008150 GO:0009888 GO:0009914 GO:0009926 GO:0009987 GO:0010087 GO:0010089 GO:0010487 GO:0010817 GO:0016740 GO:0016765 GO:0016768 GO:0030154 GO:0032502 GO:0044424 GO:0044464 GO:0048759 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:1905177

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

351

Amino Acids

39.71

Weight (kDa)

5.44

Isoelectric Point (pI)

35.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	44 - 97	6.1e-13	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	100 - 281	1.8e-42	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19530 AT5G19530
fragaria_vesca	FvH4_1g08290 FvH4_1g08290 FvH4_1g08290 FvH4_2g31200 FvH4_2g31200
malus_domestica	MD08G1070200.v1.1 MD15G1052000.v1.1
prunus_persica	Prupe.1G405900_v2.0.a1 Prupe.1G405900_v2.0.a1 Prupe.7G202400_v2.0.a1 Prupe.7G202400_v2.0.a1
pyrus_communis	pycom08g05810 pycom15g04970
rosa_chinensis	RchiOBHm_Chr2g0094361 RchiOBHm_Chr6g0311611
rosa_laevigata	RLG00000010387
rosa_multiflora	Rmu_sc0002888.1_g000005 Rmu_sc0015881.1_g000007
rosa_roxburghii	Rroxscaffold_2G00147190 Rroxscaffold_7G00157440
rosa_rugosa	Rorug02G0043600 Rorug06G0390100
rosa_samantha	Rh2AG091400 Rh2BG091400 Rh2DG090200 Rh6AG505300 Rh6BG516000 Rh6CG522000 Rh6DG507700
rosa_wichuraiana	Rw6G043980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	530
AccB1I	GGYRCC	1 cut(s)	781
AccI	GTMKAC	1 cut(s)	993
AciI	CCGC	2 cut(s)	472, 545
AclWI	GGATC	1 cut(s)	62
AcsI	RAATTY	5 cut(s)	50, 299, 569, 654, 1044
AfaI	GTAC	3 cut(s)	84, 215, 258
AfiI	CCNNNNNNNGG	2 cut(s)	608, 730
AgsI	TTSAA	3 cut(s)	608, 772, 919
AjnI	CCWGG	3 cut(s)	128, 216, 696
AluBI	AGCT	6 cut(s)	227, 392, 517, 670, 794, 907
AluI	AGCT	6 cut(s)	227, 392, 517, 670, 794, 907
Alw21I	GWGCWC	1 cut(s)	928
AlwI	GGATC	1 cut(s)	62
AoxI	GGCC	1 cut(s)	834
ApeKI	GCWGC	3 cut(s)	193, 392, 791
ApoI	RAATTY	5 cut(s)	50, 299, 569, 654, 1044
AspS9I	GGNCC	2 cut(s)	614, 704
AsuC2I	CCSGG	1 cut(s)	602
AsuHPI	GGTGA	4 cut(s)	17, 335, 712, 730
AvaII	GGWCC	2 cut(s)	614, 704
BanI	GGYRCC	1 cut(s)	781
BbsI	GAAGAC	2 cut(s)	727, 760
Bbv12I	GWGCWC	1 cut(s)	928
BbvI	GCAGC	3 cut(s)	180, 379, 803
BccI	CCATC	4 cut(s)	263, 811, 819, 893
BciT130I	CCWGG	3 cut(s)	130, 218, 698
BcnI	CCSGG	1 cut(s)	602
BfaI	CTAG	1 cut(s)	207
BisI	GCNGC	4 cut(s)	194, 393, 472, 792
BlsI	GCNGC	4 cut(s)	195, 394, 473, 793
Bme1390I	CCNGG	4 cut(s)	130, 218, 602, 698
Bme18I	GGWCC	2 cut(s)	614, 704
BmgT120I	GGNCC	2 cut(s)	614, 704
BmiI	GGNNCC	1 cut(s)	783
BmrFI	CCNGG	4 cut(s)	130, 218, 602, 698
BmsI	GCATC	3 cut(s)	267, 526, 997
BpiI	GAAGAC	2 cut(s)	727, 760
Bpu10I	CCTNAGC	1 cut(s)	666
BpuEI	CTTGAG	1 cut(s)	392
BpuMI	CCSGG	1 cut(s)	602
Bsa29I	ATCGAT	1 cut(s)	447
BsaBI	GATNNNNATC	1 cut(s)	153
BsaJI	CCNNGG	4 cut(s)	540, 696, 837, 1021
Bsc4I	CCNNNNNNNGG	2 cut(s)	608, 730
Bse1I	ACTGG	1 cut(s)	426
Bse8I	GATNNNNATC	1 cut(s)	153
BseBI	CCWGG	3 cut(s)	130, 218, 698
BseCI	ATCGAT	1 cut(s)	447
BseDI	CCNNGG	4 cut(s)	540, 696, 837, 1021
BseGI	GGATG	3 cut(s)	301, 325, 830
BseJI	GATNNNNATC	1 cut(s)	153
BseLI	CCNNNNNNNGG	2 cut(s)	608, 730
BseMII	CTCAG	3 cut(s)	855, 941, 990
BseNI	ACTGG	1 cut(s)	426
BseXI	GCAGC	3 cut(s)	180, 379, 803
BsgI	GTGCAG	2 cut(s)	306, 315
BshFI	GGCC	1 cut(s)	836
BshNI	GGYRCC	1 cut(s)	781
BshVI	ATCGAT	1 cut(s)	447
BsiHKAI	GWGCWC	1 cut(s)	928
BsiSI	CCGG	1 cut(s)	602
BslI	CCNNNNNNNGG	2 cut(s)	608, 730
BsmI	GAATGC	1 cut(s)	320
BsnI	GGCC	1 cut(s)	836
Bsp1286I	GDGCHC	1 cut(s)	928
Bsp143I	GATC	3 cut(s)	67, 154, 448
Bsp19I	CCATGG	1 cut(s)	1021
BspACI	CCGC	2 cut(s)	472, 545
BspANI	GGCC	1 cut(s)	836
BspCNI	CTCAG	3 cut(s)	856, 940, 991
BspDI	ATCGAT	1 cut(s)	447
BspHI	TCATGA	1 cut(s)	310
BspLI	GGNNCC	1 cut(s)	783
BspPI	GGATC	1 cut(s)	62
BspT107I	GGYRCC	1 cut(s)	781
BsrI	ACTGG	1 cut(s)	426
BssECI	CCNNGG	4 cut(s)	540, 696, 837, 1021
BssMI	GATC	3 cut(s)	67, 154, 448
BssT1I	CCWWGG	2 cut(s)	540, 1021
Bst2UI	CCWGG	3 cut(s)	130, 218, 698
Bst4CI	ACNGT	4 cut(s)	95, 188, 487, 961
Bst6I	CTCTTC	1 cut(s)	551
BstC8I	GCNNGC	2 cut(s)	476, 712
BstDEI	CTNAG	4 cut(s)	666, 864, 927, 999
BstDSI	CCRYGG	1 cut(s)	1021
BstF5I	GGATG	3 cut(s)	301, 325, 830
BstKTI	GATC	3 cut(s)	70, 157, 451
BstMBI	GATC	3 cut(s)	67, 154, 448
BstMWI	GCNNNNNNNGC	2 cut(s)	401, 707
BstNI	CCWGG	3 cut(s)	130, 218, 698
BstSCI	CCNGG	4 cut(s)	128, 216, 600, 696
BstV1I	GCAGC	3 cut(s)	180, 379, 803
BstV2I	GAAGAC	2 cut(s)	727, 760
BstXI	CCANNNNNNTGG	1 cut(s)	1028
Bsu15I	ATCGAT	1 cut(s)	447
BsuRI	GGCC	1 cut(s)	836
BsuTUI	ATCGAT	1 cut(s)	447
BtgI	CCRYGG	1 cut(s)	1021
BtsCI	GGATG	3 cut(s)	301, 325, 830
BtsI	GCAGTG	1 cut(s)	84
BtsIMutI	CAGTG	4 cut(s)	61, 84, 492, 996
Cac8I	GCNNGC	2 cut(s)	476, 712
CciI	TCATGA	1 cut(s)	310
Cfr13I	GGNCC	2 cut(s)	614, 704
ClaI	ATCGAT	1 cut(s)	447
Csp6I	GTAC	3 cut(s)	83, 214, 257
CviQI	GTAC	3 cut(s)	83, 214, 257
DdeI	CTNAG	4 cut(s)	666, 864, 927, 999
DpnI	GATC	3 cut(s)	69, 156, 450
DpnII	GATC	3 cut(s)	67, 154, 448
Eam1104I	CTCTTC	1 cut(s)	551
EarI	CTCTTC	1 cut(s)	551
EciI	GGCGGA	1 cut(s)	560
Eco130I	CCWWGG	2 cut(s)	540, 1021
Eco32I	GATATC	1 cut(s)	577
Eco47I	GGWCC	2 cut(s)	614, 704
EcoO109I	RGGNCCY	1 cut(s)	614
EcoRII	CCWGG	3 cut(s)	128, 216, 696
EcoRV	GATATC	1 cut(s)	577
EcoT14I	CCWWGG	2 cut(s)	540, 1021
ErhI	CCWWGG	2 cut(s)	540, 1021
FauNDI	CATATG	1 cut(s)	787
FblI	GTMKAC	1 cut(s)	993
Fnu4HI	GCNGC	4 cut(s)	194, 393, 472, 792
FokI	GGATG	3 cut(s)	308, 312, 837
Fsp4HI	GCNGC	4 cut(s)	194, 393, 472, 792
FspBI	CTAG	1 cut(s)	207
GluI	GCNGC	4 cut(s)	194, 393, 472, 792
HaeIII	GGCC	1 cut(s)	836
HapII	CCGG	1 cut(s)	602
HincII	GTYRAC	1 cut(s)	184
HindII	GTYRAC	1 cut(s)	184
HindIII	AAGCTT	2 cut(s)	515, 668
HinfI	GANTC	1 cut(s)	490
HpaII	CCGG	1 cut(s)	602
HphI	GGTGA	4 cut(s)	17, 335, 712, 730
Hpy166II	GTNNAC	2 cut(s)	184, 994
Hpy188I	TCNGA	2 cut(s)	159, 841
Hpy188III	TCNNGA	4 cut(s)	250, 311, 452, 494
Hpy8I	GTNNAC	2 cut(s)	184, 994
HpyAV	CCTTC	4 cut(s)	247, 377, 602, 652
HpyCH4III	ACNGT	4 cut(s)	95, 188, 487, 961
HpyCH4IV	ACGT	1 cut(s)	358
HpyF10VI	GCNNNNNNNGC	2 cut(s)	401, 707
HpyF3I	CTNAG	4 cut(s)	666, 864, 927, 999
HpySE526I	ACGT	1 cut(s)	358
Kzo9I	GATC	3 cut(s)	67, 154, 448
LmnI	GCTCC	1 cut(s)	904
Lsp1109I	GCAGC	3 cut(s)	180, 379, 803
LweI	GCATC	3 cut(s)	267, 526, 997
MaeI	CTAG	1 cut(s)	207
MaeII	ACGT	1 cut(s)	358
MaeIII	GTNAC	4 cut(s)	341, 430, 440, 691
MalI	GATC	3 cut(s)	69, 156, 450
MboI	GATC	3 cut(s)	67, 154, 448
MboII	GAAGA	6 cut(s)	149, 286, 568, 727, 760, 1016
MhlI	GDGCHC	1 cut(s)	928
MseI	TTAA	4 cut(s)	519, 660, 672, 759
MslI	CAYNNNNRTG	4 cut(s)	337, 689, 823, 1026
MspA1I	CMGCKG	1 cut(s)	392
MspI	CCGG	1 cut(s)	602
MspR9I	CCNGG	4 cut(s)	130, 218, 602, 698
Mva1269I	GAATGC	1 cut(s)	320
MvaI	CCWGG	3 cut(s)	130, 218, 698
MwoI	GCNNNNNNNGC	2 cut(s)	401, 707
NciI	CCSGG	1 cut(s)	602
NcoI	CCATGG	1 cut(s)	1021
NdeI	CATATG	1 cut(s)	787
NdeII	GATC	3 cut(s)	67, 154, 448
NlaIV	GGNNCC	1 cut(s)	783
NmuCI	GTSAC	3 cut(s)	341, 440, 691
PagI	TCATGA	1 cut(s)	310
PctI	GAATGC	1 cut(s)	320
PfeI	GAWTC	1 cut(s)	490
PkrI	GCNGC	4 cut(s)	195, 394, 473, 793
PpuMI	RGGWCCY	1 cut(s)	614
PsiI	TTATAA	1 cut(s)	530
Psp5II	RGGWCCY	1 cut(s)	614
Psp6I	CCWGG	3 cut(s)	128, 216, 696
PspGI	CCWGG	3 cut(s)	128, 216, 696
PspN4I	GGNNCC	1 cut(s)	783
PspPI	GGNCC	2 cut(s)	614, 704
PspPPI	RGGWCCY	1 cut(s)	614
PvuII	CAGCTG	1 cut(s)	392
RsaI	GTAC	3 cut(s)	84, 215, 258
RsaNI	GTAC	3 cut(s)	83, 214, 257
RseI	CAYNNNNRTG	4 cut(s)	337, 689, 823, 1026
SaqAI	TTAA	4 cut(s)	519, 660, 672, 759
SatI	GCNGC	4 cut(s)	194, 393, 472, 792
Sau3AI	GATC	3 cut(s)	67, 154, 448
Sau96I	GGNCC	2 cut(s)	614, 704
ScrFI	CCNGG	4 cut(s)	130, 218, 602, 698
SduI	GDGCHC	1 cut(s)	928
SfaNI	GCATC	3 cut(s)	267, 526, 997
SinI	GGWCC	2 cut(s)	614, 704
SmiMI	CAYNNNNRTG	4 cut(s)	337, 689, 823, 1026
SmlI	CTYRAG	1 cut(s)	407
SmoI	CTYRAG	1 cut(s)	407
SsiI	CCGC	2 cut(s)	472, 545
SspMI	CTAG	1 cut(s)	207
StyD4I	CCNGG	4 cut(s)	128, 216, 600, 696
StyI	CCWWGG	2 cut(s)	540, 1021
TaaI	ACNGT	4 cut(s)	95, 188, 487, 961
TaiI	ACGT	1 cut(s)	361
TaqI	TCGA	1 cut(s)	447
TauI	GCSGC	1 cut(s)	474
TfiI	GAWTC	1 cut(s)	490
Tru1I	TTAA	4 cut(s)	519, 660, 672, 759
Tru9I	TTAA	4 cut(s)	519, 660, 672, 759
TscAI	CASTG	4 cut(s)	61, 84, 492, 1003
TseFI	GTSAC	3 cut(s)	341, 440, 691
TseI	GCWGC	3 cut(s)	193, 392, 791
Tsp45I	GTSAC	3 cut(s)	341, 440, 691
TspDTI	ATGAA	7 cut(s)	150, 312, 327, 355, 922, 965, 996
TspGWI	ACGGA	1 cut(s)	48
TspRI	CASTG	4 cut(s)	61, 84, 492, 1003
VpaK11BI	GGWCC	2 cut(s)	614, 704
XapI	RAATTY	5 cut(s)	50, 299, 569, 654, 1044
XcmI	CCANNNNNNNNNTGG	1 cut(s)	547
XmiI	GTMKAC	1 cut(s)	993
XspI	CTAG	1 cut(s)	207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.