Rh2AG091400

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004438
Physical Location & Seq
Reverse (-)
7673079 .. 7674863
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG091400.1

Sequence Viewer

Length: 405 bp
ATGTGGTCGTTTCTCTGTGCTGCTGTCATGGCGTTCTATACTCCTCTGCCTCGGCTTCCACTTGACGAACATCCTTCGAACTCGTTTGGTGTTTTAGGTTCTGTAATTGGTAATTTCAGCCCCAAAACCATATTCATAATGGGAGGTGGTGAAGGGTCAACAGCAAGGGAGGCACTGAAGCACAAAGATGTGGAGAAAGTTATCATGTGTGATATAGATGAGACTGGTCCAGCAGGAATTCTTTCTCATAAGGCTATCTTCACTTCAATATACAACACTGTAAAACAAGTGTTCAAATGCATGCTTTTGAAAGGAACTAATGTCTTCACTGAAGAAACTACAAAGTTCTGCCAGGGACATGGAGTCACTGATGATGCACAAGGCAATGAGGTGATTGAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

134

Amino Acids

14.57

Weight (kDa)

5.48

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 40 - 74 1.4e-08 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 237
AcuI CTGAAG 2 cut(s) 197, 351
AgsI TTSAA 3 cut(s) 267, 295, 310
AhdI GACNNNNNGTC 1 cut(s) 362
AjnI CCWGG 1 cut(s) 351
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
Alw26I GTCTC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 237
Asp700I GAANNNNTTC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 161, 403
AsuII TTCGAA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 316
BbvI GCAGC 1 cut(s) 7
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 1 cut(s) 215
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 227
BmeRI GACNNNNNGTC 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 227
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 316
Bpu14I TTCGAA 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 50, 352
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 50, 352
BseGI GGATG 1 cut(s) 70
BseMI GCAATG 1 cut(s) 391
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 1 cut(s) 7
BslFI GGGAC 1 cut(s) 369
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 77
BspT104I TTCGAA 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 391
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 50, 352
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 302
BstF5I GGATG 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 215
BstMWI GCNNNNNNNGC 2 cut(s) 29, 170
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 316
BstXI CCANNNNNNTGG 1 cut(s) 359
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 4 cut(s) 173, 276, 327, 366
Cac8I GCNNGC 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 227
CviAII CATG 4 cut(s) 28, 205, 301, 359
CviJI RGCY 3 cut(s) 55, 120, 254
CviKI_1 RGCY 3 cut(s) 55, 120, 254
DriI GACNNNNNGTC 1 cut(s) 362
Eam1105I GACNNNNNGTC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 197, 351
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 302
FaeI CATG 4 cut(s) 31, 208, 304, 362
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 1 cut(s) 369
FatI CATG 4 cut(s) 27, 204, 300, 358
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 21
Hin1II CATG 4 cut(s) 31, 208, 304, 362
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HinfI GANTC 1 cut(s) 363
HphI GGTGA 2 cut(s) 161, 403
Hpy166II GTNNAC 1 cut(s) 159
Hpy8I GTNNAC 1 cut(s) 159
HpyAV CCTTC 2 cut(s) 84, 146
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 2 cut(s) 300, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 170
Hsp92II CATG 4 cut(s) 31, 208, 304, 362
LpnPI CCDG 5 cut(s) 210, 219, 243, 338, 365
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 364
MaeIII GTNAC 1 cut(s) 364
MboII GAAGA 3 cut(s) 250, 316, 344
MluCI AATT 3 cut(s) 105, 112, 237
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 6 cut(s) 54, 60, 137, 163, 382, 391
Mph1103I ATGCAT 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 241
MslI CAYNNNNRTG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 29, 170
NlaIII CATG 4 cut(s) 31, 208, 304, 362
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 364
NsiI ATGCAT 1 cut(s) 302
NspI RCATGY 1 cut(s) 304
NspV TTCGAA 1 cut(s) 77
PaeI GCATGC 1 cut(s) 304
PdmI GAANNNNTTC 1 cut(s) 241
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspPI GGNCC 1 cut(s) 227
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 1 cut(s) 353
SetI ASST 4 cut(s) 100, 148, 393, 402
SfaNI GCATC 1 cut(s) 364
SfuI TTCGAA 1 cut(s) 77
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 186
SphI GCATGC 1 cut(s) 304
Sse9I AATT 3 cut(s) 105, 112, 237
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 1 cut(s) 77
TasI AATT 3 cut(s) 105, 112, 237
TscAI CASTG 4 cut(s) 180, 283, 334, 373
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 124
TspRI CASTG 4 cut(s) 180, 283, 334, 373
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 237
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 241
Zsp2I ATGCAT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.