Rh2DG090200

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004438
Physical Location & Seq
Reverse (-)
6959882 .. 6961558
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG090200.1

Sequence Viewer

Length: 651 bp
ATGGAAAGCTGCAGAGTGCTGAAAATGATGCCCCAAAACCATATTCATAATGGTGAAGCGTCAACAGCAAGGGAGGCACTGAAGCACAAAGATGTGGAGAAAGTTATCATGTGTGATATAGATGAGGTGATTGTCAATTTTTGCCGCGCACATCTGGAAGAAAATCAACTAGGCTTCAACTTGTTTTCAATGATCCTAAGTGAGGCTGAGTTGGAGAAAACTGAAGAGAAGTTTGATGTGATTGTGGGAGACCTACCAGACCCAATTGAGGGAGGCCCTTGCAATAATCTTTACACCAAAAACTTTTATGAGCAAGTCATAAAACCTCACCTCAAAGACAATGGCATCTTTGTCACTCAAGCTGGTCCAGCAGGAATTCTTTCTCATAAGGCTATCTTCACTTCAATAGACAAACACTGTAAAACAAGTGTTCAAATATGTGTTGGCATACACAGCTCATGTGCCATCTTATGCAGATTCATGTGGATGGGTGATGATGGTGCTTTCATTGTCTCATCAACTGTGGTCAACAAGACGATCTACACATCGCTTTTGAAAGGAACTAATGTCTTCACTGAAGAAACTACAATGTTCTGCCAGGGACATGGAGTCACTGATGATGCACAGGGCAATGAGGTGATTGAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

216

Amino Acids

24.0

Weight (kDa)

5.12

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 20 - 146 1.3e-20 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 147
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 187
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 3 cut(s) 101, 243, 597
AfiI CCNNNNNNNGG 3 cut(s) 202, 268, 269
AgsI TTSAA 5 cut(s) 178, 189, 405, 434, 556
AhdI GACNNNNNGTC 1 cut(s) 608
AjnI CCWGG 1 cut(s) 597
AjuI GAANNNNNNNTTGG 2 cut(s) 27, 59
AluBI AGCT 3 cut(s) 9, 362, 456
AluI AGCT 3 cut(s) 9, 362, 456
Alw26I GTCTC 2 cut(s) 243, 517
AlwI GGATC 1 cut(s) 187
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 9
ApoI RAATTY 1 cut(s) 375
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 275, 365
AsuHPI GGTGA 5 cut(s) 65, 139, 320, 503, 649
AvaII GGWCC 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 562
BccI CCATC 3 cut(s) 473, 481, 491
BciT130I CCWGG 1 cut(s) 599
BcoDI GTCTC 2 cut(s) 243, 517
BfaI CTAG 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 10
BisI GCNGC 2 cut(s) 10, 145
BlsI GCNGC 2 cut(s) 11, 146
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 365
BmeRI GACNNNNNGTC 1 cut(s) 608
BmgT120I GGNCC 2 cut(s) 275, 365
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 3 cut(s) 18, 354, 610
BpiI GAAGAC 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 598
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 268, 269
Bse3DI GCAATG 1 cut(s) 637
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 3 cut(s) 202, 268, 269
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 276
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 3 cut(s) 202, 268, 269
BsmAI GTCTC 2 cut(s) 243, 517
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 192, 537
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 637
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 2 cut(s) 192, 537
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 2 cut(s) 419, 523
Bst6I CTCTTC 1 cut(s) 219
BstDEI CTNAG 2 cut(s) 197, 207
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 492
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 195, 540
BstMAI GTCTC 2 cut(s) 243, 517
BstMBI GATC 2 cut(s) 192, 537
BstMWI GCNNNNNNNGC 4 cut(s) 65, 74, 368, 453
BstNI CCWGG 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 597
BstSFI CTRYAG 1 cut(s) 10
BstUI CGCG 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 562
BstXI CCANNNNNNTGG 1 cut(s) 605
BsuRI GGCC 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 1 cut(s) 492
BtsIMutI CAGTG 4 cut(s) 77, 415, 573, 612
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 275, 365
CseI GACGC 1 cut(s) 48
CviAII CATG 4 cut(s) 109, 459, 481, 605
CviJI RGCY 7 cut(s) 9, 174, 206, 276, 362, 392, 456
CviKI_1 RGCY 7 cut(s) 9, 174, 206, 276, 362, 392, 456
DdeI CTNAG 2 cut(s) 197, 207
DpnI GATC 2 cut(s) 194, 539
DpnII GATC 2 cut(s) 192, 537
DriI GACNNNNNGTC 1 cut(s) 608
Eam1104I CTCTTC 1 cut(s) 219
Eam1105I GACNNNNNGTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 3 cut(s) 101, 243, 597
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 597
FaeI CATG 4 cut(s) 112, 462, 484, 608
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 615
FatI CATG 4 cut(s) 108, 458, 480, 604
Fnu4HI GCNGC 2 cut(s) 10, 145
FokI GGATG 1 cut(s) 499
Fsp4HI GCNGC 2 cut(s) 10, 145
FspBI CTAG 1 cut(s) 170
GlaI GCGC 1 cut(s) 148
GluI GCNGC 2 cut(s) 10, 145
HaeIII GGCC 1 cut(s) 276
HgaI GACGC 1 cut(s) 48
HhaI GCGC 1 cut(s) 149
Hin1II CATG 4 cut(s) 112, 462, 484, 608
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 2 cut(s) 63, 529
HindII GTYRAC 2 cut(s) 63, 529
HinfI GANTC 2 cut(s) 477, 609
HphI GGTGA 5 cut(s) 65, 139, 320, 503, 649
Hpy166II GTNNAC 2 cut(s) 63, 529
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 2 cut(s) 63, 529
HpyCH4III ACNGT 2 cut(s) 419, 523
HpyCH4V TGCA 4 cut(s) 12, 282, 474, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 74, 368, 453
HpyF3I CTNAG 2 cut(s) 197, 207
Hsp92II CATG 4 cut(s) 112, 462, 484, 608
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 2 cut(s) 192, 537
LpnPI CCDG 8 cut(s) 140, 270, 348, 357, 381, 584, 611, 611
LweI GCATC 3 cut(s) 18, 354, 610
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 2 cut(s) 352, 610
MalI GATC 2 cut(s) 194, 539
MboI GATC 2 cut(s) 192, 537
MboII GAAGA 5 cut(s) 170, 236, 388, 562, 590
MfeI CAATTG 1 cut(s) 264
MluCI AATT 3 cut(s) 136, 264, 375
MlyI GAGTC 1 cut(s) 618
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 9 cut(s) 67, 118, 196, 262, 266, 336, 341, 628, 637
MroXI GAANNNNTTC 1 cut(s) 379
MslI CAYNNNNRTG 3 cut(s) 51, 90, 485
MspR9I CCNGG 1 cut(s) 599
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 65, 74, 368, 453
NdeII GATC 2 cut(s) 192, 537
NlaIII CATG 4 cut(s) 112, 462, 484, 608
NmuCI GTSAC 2 cut(s) 352, 610
PdmI GAANNNNTTC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 477
PkrI GCNGC 2 cut(s) 11, 146
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspPI GGNCC 2 cut(s) 275, 365
PstI CTGCAG 1 cut(s) 14
RseI CAYNNNNRTG 3 cut(s) 51, 90, 485
SatI GCNGC 2 cut(s) 10, 145
Sau3AI GATC 2 cut(s) 192, 537
Sau96I GGNCC 2 cut(s) 275, 365
SchI GAGTC 1 cut(s) 618
ScrFI CCNGG 1 cut(s) 599
SetI ASST 9 cut(s) 11, 129, 255, 328, 333, 364, 458, 639, 648
SfaNI GCATC 3 cut(s) 18, 354, 610
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 3 cut(s) 51, 90, 485
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 3 cut(s) 136, 264, 375
SsiI CCGC 1 cut(s) 145
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 597
TaaI ACNGT 2 cut(s) 419, 523
TasI AATT 3 cut(s) 136, 264, 375
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 1 cut(s) 477
TscAI CASTG 4 cut(s) 84, 422, 580, 619
TseFI GTSAC 2 cut(s) 352, 610
TseI GCWGC 1 cut(s) 9
Tsp45I GTSAC 2 cut(s) 352, 610
TspDTI ATGAA 3 cut(s) 35, 469, 496
TspRI CASTG 4 cut(s) 84, 422, 580, 619
VpaK11BI GGWCC 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 379
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.